Standard name
Human Ortholog
Description Zinc-finger protein of unknown function; possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids; STP3 has a paralog, STP4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.08 0.05 0.05 0 0.05 0.05 0 0 0 0 0.09 0.15 0.06 0.06 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.45 0.27 0.07 0 0.23 0.34 0.22 0.43 0.4 0.6 0.4 0 0 0 0 0.05 0 0 0 0 0 0 0.05
Nucleus 0.89 0.85 0.93 0.87 0.92 0.89 0.87 0.89 0.85 0.85 0.81 0.89 0.96 0.9 0.82 0.8 0.81 0.82 0.85 0.84 0.79 0.74 0.64 0.51
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.16 0.1 0.06 0.09 0.1 0.11 0.18 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 1 10 3 3 4 2 0 0 0 0 0 0 3 0 0 1 2 4
Bud Neck 0 1 0 0 1 0 0 1 0 3 0 0 0 0 1 0 0 0 0 0 0 1 2 3
Bud Site 0 0 0 0 1 0 8 3 11 9 7 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 13 0 2 14 19 14 17 23 15 9 9 4 9 29 48 15 9 14 2 0 1 3 7 9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 16 3 10 0 0 0 1 0 0
Endosome 1 0 0 1 0 0 1 0 0 0 0 0 0 0 2 2 1 4 4 0 0 0 3 5
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 1 1 0 0
Mitochondria 50 9 8 12 11 65 180 104 142 159 174 143 4 6 1 10 7 5 3 0 0 1 6 19
Nucleus 266 17 28 151 331 251 457 414 281 337 232 321 228 282 253 191 116 151 252 33 43 108 219 193
Nuclear Periphery 0 0 0 0 0 1 1 7 3 3 2 1 0 0 0 0 1 0 1 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 5 4 13 11 14 9 0 0 0 0 0 1 3 0 0 6 20 34
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 4 2 2 14 3 3 1 5 4 0 4 0 0 1 1 1 1 2 7 14
Vac/Vac Membrane 3 0 0 1 3 3 6 2 1 2 3 4 0 4 10 39 23 19 17 3 5 16 61 82
Unique Cell Count 298 20 30 174 360 283 525 467 329 396 288 362 237 314 310 239 144 184 297 40 55 148 341 382
Labelled Cell Count 333 27 38 182 371 337 679 584 472 539 446 496 246 322 320 273 160 205 297 40 55 148 341 382


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.1 4.7 5.3 5.5 4.5 4.2 4.8 4.2 4.4 4.0 4.4 5.9 5.7 5.6 6.7 7.0 6.9 5.2 5.2 5.6
Std Deviation (1e-4) 0.7 0.9 0.7 1.4 1.4 1.3 1.1 1.1 1.2 1.0 1.2 1.0 0.8 0.9 1.1 1.1 1.0 1.5 1.4 1.1 1.3
Intensity Change (Log2) – – – 0.17 0.24 -0.04 -0.15 0.05 -0.15 -0.1 -0.23 -0.07 0.34 0.3 0.26 0.52 0.58 0.57 0.17 0.17 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7867 1.4915 1.2367 1.4645 1.2228 1.3445 0.5931 1.2064 0.8864 0.4767 0.5806 0.9015 -0.0284 0.1274 -0.1263 0.7142 0.0082 0.2411
Actin 0.0335 0 0.0068 0.0282 0.0076 0.0076 0.0122 0 0 0 0.0298 0 0.017 0.0001 0.0053 0.0255 0.0016 0.0345
Bud 0.0007 0 0.0002 0.0078 0.0001 0.0001 0.0001 0.0001 0 0 0.0003 0.0001 0.0007 0.0001 0.0001 0.0014 0.0002 0.0003
Bud Neck 0.0051 0.0001 0.0004 0.0056 0.0018 0.0014 0.0009 0.0001 0.0001 0.0001 0.0005 0.0008 0.0075 0.0001 0.0004 0.0015 0.0003 0.0009
Bud Periphery 0.0016 0 0.0007 0.0202 0.0002 0.0002 0.0001 0.0004 0 0 0.0004 0.0001 0.0012 0.0002 0.0001 0.0053 0.0002 0.0006
Bud Site 0.0095 0 0.001 0.0073 0.0008 0.0004 0.0004 0.0001 0 0 0.0009 0.0001 0.0053 0.0004 0.0002 0.0021 0.0007 0.0005
Cell Periphery 0.0004 0 0.0001 0.0009 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0 0.002 0.0002 0 0.0013 0.0001 0.0001
Cytoplasm 0.0093 0.0002 0.0015 0.0012 0.0044 0.001 0.0022 0.0005 0.0007 0.0001 0.0055 0.0002 0.0197 0.0149 0.0028 0.0003 0.0003 0.0005
Cytoplasmic Foci 0.0232 0 0.0072 0.0015 0.0139 0.0023 0.0004 0 0 0 0.0106 0 0.0075 0.0011 0.0074 0.0033 0.0155 0.0064
Eisosomes 0.0004 0 0.0001 0.0009 0.0001 0.0001 0.0005 0 0 0 0.0005 0 0.0005 0 0 0.0002 0 0.0001
Endoplasmic Reticulum 0.0046 0 0.0006 0.0014 0.0002 0.0014 0.001 0 0 0 0.0012 0 0.0078 0.0017 0.0006 0.0003 0.0003 0.0009
Endosome 0.0206 0 0.0048 0.0076 0.042 0.0049 0.0021 0.0013 0 0 0.025 0 0.0224 0.0024 0.0049 0.0107 0.0628 0.0351
Golgi 0.0085 0 0.0025 0.0042 0.0148 0.0046 0.0009 0 0 0 0.0048 0 0.0053 0.0001 0.0081 0.0171 0.0391 0.0261
Lipid Particles 0.0206 0 0.0041 0.0056 0.0179 0.0046 0.001 0 0 0 0.0186 0 0.0117 0.0002 0.0018 0.0067 0.0193 0.0077
Mitochondria 0.0247 0.0001 0.0172 0.0164 0.046 0.0014 0.0017 0.0016 0.0001 0.0001 0.0048 0 0.0295 0.0005 0.0135 0.0512 0.0167 0.0197
None 0.004 0.0002 0.0009 0.0009 0.0142 0.0025 0.0067 0.0001 0.0007 0 0.0663 0 0.032 0.0011 0.0154 0.0001 0.0001 0.0003
Nuclear Periphery 0.0162 0.0034 0.007 0.0028 0.0081 0.0019 0.0033 0.002 0.0027 0.0004 0.0148 0.0003 0.0218 0.0037 0.003 0.0014 0.0005 0.0027
Nucleolus 0.0244 0.0145 0.0101 0.0076 0.034 0.0205 0.0058 0.006 0.0071 0.008 0.0129 0.0144 0.0083 0.0048 0.0053 0.0125 0.0086 0.0082
Nucleus 0.7556 0.9736 0.9217 0.8671 0.7142 0.9311 0.9487 0.9741 0.9668 0.9909 0.6996 0.9801 0.7667 0.9487 0.9262 0.7922 0.8242 0.8481
Peroxisomes 0.0205 0 0.0042 0.0048 0.0172 0.0031 0.0004 0 0 0 0.0133 0 0.013 0 0.0013 0.0033 0.0044 0.0042
Punctate Nuclear 0.0114 0.0076 0.0055 0.001 0.0556 0.0092 0.011 0.0013 0.0217 0.0003 0.0879 0.0037 0.0137 0.0031 0.0032 0.0003 0.0003 0.0007
Vacuole 0.0032 0 0.0017 0.0046 0.005 0.0014 0.0002 0.0078 0 0 0.0012 0.0001 0.0046 0.013 0.0002 0.0559 0.0025 0.0009
Vacuole Periphery 0.0021 0 0.0017 0.0024 0.0016 0.0003 0.0002 0.0045 0 0 0.0009 0 0.0019 0.0037 0.0001 0.0072 0.0022 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.2644 24.8506 28.3695 31.843 33.9632 33.1266 43.9712 39.1353 35.7495 37.9146
Translational Efficiency 0.8595 0.9997 0.7496 0.5461 0.7089 0.7906 0.4639 0.7084 0.5638 0.4906

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1538 912 2368 1247 1777 1296 2053 890 3315 2208 4421 2137

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 702.91 738.99 850.41 875.76 719.61 776.36 901.99 927.53 711.86 760.92 874.36 897.32
Standard Deviation 94.03 109.41 114.97 117.88 95.00 105.53 116.48 114.99 94.92 108.72 118.50 119.44
Intensity Change Log 2 — 0.072215 0.274819 0.317196 — 0.109511 0.325896 0.366179 — 0.091202 0.300883 0.342182

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000187 0.000565 0.000585 0.001143 0.000103 0.000310 0.000360 0.001302 0.000142 0.000415 0.000480 0.001209
Bud Neck 0.002737 0.004584 0.031594 0.013401 0.002521 0.004283 0.013021 0.016044 0.002621 0.004407 0.022969 0.014502
Bud Site 0.001055 0.002018 0.004540 0.004628 0.000406 0.002437 0.001506 0.012387 0.000707 0.002264 0.003131 0.007859
Cell Periphery 0.000128 0.000178 0.000160 0.000133 0.000081 0.000120 0.000066 0.000114 0.000102 0.000144 0.000116 0.000125
Cytoplasm 0.030062 0.027457 0.005201 0.014264 0.016745 0.015697 0.002853 0.023864 0.022923 0.020555 0.004111 0.018262
Cytoplasmic Foci 0.005392 0.003824 0.000150 0.002031 0.002531 0.002608 0.000199 0.003420 0.003858 0.003110 0.000173 0.002609
Eisosomes 0.000022 0.000026 0.000032 0.000023 0.000013 0.000016 0.000020 0.000028 0.000017 0.000020 0.000026 0.000025
Endoplasmic Reticulum 0.002388 0.004497 0.005284 0.010112 0.002102 0.003011 0.003552 0.007731 0.002235 0.003625 0.004480 0.009121
Endosome 0.000445 0.001188 0.000518 0.002410 0.000504 0.001497 0.000140 0.003840 0.000477 0.001369 0.000342 0.003005
Golgi 0.000223 0.000619 0.000113 0.001258 0.000373 0.001241 0.000040 0.001998 0.000304 0.000984 0.000079 0.001566
Lipid Particles 0.001073 0.001129 0.000108 0.001508 0.000713 0.001065 0.000054 0.000332 0.000880 0.001091 0.000083 0.001018
Mitochondria 0.002639 0.002570 0.003164 0.002025 0.002511 0.003461 0.000748 0.005777 0.002570 0.003093 0.002042 0.003588
Mitotic Spindle 0.001516 0.001800 0.004974 0.003909 0.001708 0.007005 0.000258 0.014788 0.001619 0.004855 0.002784 0.008440
None 0.012543 0.012340 0.002877 0.006035 0.005449 0.004205 0.003021 0.006447 0.008740 0.007565 0.002944 0.006207
Nuclear Periphery 0.000733 0.000775 0.001737 0.002038 0.000971 0.000987 0.000293 0.001399 0.000860 0.000899 0.001066 0.001772
Nuclear Periphery Foci 0.000458 0.000609 0.000733 0.001352 0.000269 0.000615 0.000071 0.000351 0.000357 0.000612 0.000426 0.000935
Nucleolus 0.007431 0.010395 0.005405 0.002108 0.006139 0.006074 0.004199 0.002248 0.006739 0.007859 0.004845 0.002166
Nucleus 0.928486* 0.921016* 0.928919* 0.920996* 0.954989* 0.941885* 0.968575* 0.885010* 0.942693* 0.933265* 0.947335* 0.906009*
Peroxisomes 0.000909 0.001957 0.000154 0.000135 0.000455 0.001232 0.000075 0.000560 0.000666 0.001531 0.000118 0.000312
Vacuole 0.001353 0.002102 0.003037 0.008185 0.001033 0.001773 0.000768 0.010445 0.001182 0.001909 0.001983 0.009126
Vacuole Periphery 0.000221 0.000351 0.000716 0.002306 0.000386 0.000477 0.000180 0.001916 0.000309 0.000425 0.000467 0.002144

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.44 -11.34 -5.70 -2.98 -2.82 -2.46 -15.18 -6.71 -5.13 -5.06 -4.54 -17.42 -8.76 -5.88 -5.48
Bud Neck -2.49 -18.56 -11.91 -10.35 8.78 -2.52 -11.25 -10.62 -9.36 -2.81 -3.47 -21.64 -16.11 -13.95 4.78
Bud Site -1.66 -5.30 -3.36 -1.74 0.47 -2.71 -2.53 -4.50 -3.10 -3.73 -3.09 -6.05 -5.72 -3.44 -2.80
Cell Periphery -1.65 -0.73 0.68 2.24 1.45 -0.85 1.91 -2.78 0.24 -5.16 -1.40 -0.41 -0.70 1.12 -0.23
Cytoplasm 1.04 10.08 6.70 4.57 -4.33 0.51 8.48 -1.89 -2.21 -8.28 1.45 12.92 3.80 1.99 -9.09
Cytoplasmic Foci 1.53 6.29 4.59 2.75 -1.59 0.10 3.32 1.33 1.23 -1.54 1.44 7.11 4.46 2.97 -2.14
Eisosomes -1.39 -4.28 -2.61 -0.69 2.35 -1.58 -6.16 -12.49 -9.81 -9.73 -1.76 -7.29 -11.03 -7.96 -5.45
Endoplasmic Reticulum -5.32 -13.11 -15.20 -9.56 -9.01 -3.36 -9.27 -17.02 -14.48 -14.09 -6.09 -16.14 -22.39 -17.04 -15.69
Endosome -2.03 0.21 -2.14 -0.37 -2.20 -2.33 3.40 -2.29 -0.80 -2.83 -3.07 2.32 -3.13 -0.76 -3.61
Golgi -1.58 1.81 -1.37 -0.19 -1.75 -1.53 1.56 -0.95 0.63 -1.75 -1.96 1.97 -1.54 0.57 -2.49
Lipid Particles -0.16 6.62 0.43 0.48 -1.36 -0.91 3.55 1.16 1.69 -7.36 -0.81 6.63 0.54 1.00 -1.93
Mitochondria 0.02 -0.48 0.61 0.77 2.29 -0.96 2.15 -1.01 -0.22 -3.02 -0.76 0.87 -0.32 0.54 -1.61
Mitotic Spindle -0.44 -2.81 -2.68 -2.40 -0.33 -2.61 2.04 -3.85 -1.49 -4.83 -2.61 -1.42 -4.61 -1.90 -3.80
None 0.11 6.83 4.90 4.07 -2.36 1.40 3.03 0.33 -1.03 -2.63 1.10 7.40 4.22 2.80 -3.42
Nuclear Periphery -0.32 -2.92 -4.96 -4.81 -1.60 -0.08 7.55 -10.53 -9.78 -16.03 -0.37 -1.15 -7.98 -7.67 -5.54
Nuclear Periphery Foci -0.85 -0.32 -0.87 -0.34 -0.61 -1.58 3.21 -3.13 -1.11 -4.27 -1.65 0.20 -1.94 -0.84 -1.87
Nucleolus -2.71 1.82 5.62 7.65 5.38 0.05 2.14 4.37 3.14 2.59 -1.61 2.62 7.14 7.27 5.78
Nucleus 0.91 0.04 1.23 0.15 1.38 2.27 -3.09 10.62 7.94 12.86 1.95 -0.92 8.70 5.94 10.15
Peroxisomes -1.30 3.66 3.88 2.49 1.50 -1.80 3.70 1.19 2.14 -2.74 -2.10 4.94 4.19 3.38 -1.85
Vacuole -1.33 -3.82 -6.62 -6.02 -5.02 -1.68 0.88 -9.30 -8.93 -9.40 -2.06 -3.43 -11.38 -10.72 -10.46
Vacuole Periphery -1.30 -2.11 -1.88 -1.65 -1.18 -0.47 1.24 -0.72 -0.56 -1.09 -0.98 -0.73 -1.88 -1.59 -1.65
Standard name
Human Ortholog
Description Zinc-finger protein of unknown function; possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids; STP3 has a paralog, STP4, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Stp3

Stp3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stp3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available