Standard name
Human Ortholog
Description Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 – 0.07 0.06 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.05 0.05 0.2 0.16 0.22 0.1 0.19 – 0.12 0.08 0.09 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0.05 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.27 0.34 0.39 0.32 0.35 0.32 – 0.24 0.26 0.27 0.3 0.35 0.34 0.41 0.17 0.17 0.24 0.09 0.1 0.11 0.22 0.2 0.15
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.09 0.08 0 0 0 0 0 – 0 0 0 0 0.23 0.49 0.38 0.22 0.32 0.21 0.14 0.11 0.06 0.08 0.07 0.1
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0 0 0 0.08 0.07 – 0.31 0.33 0.35 0.18 0.09 0 0 0.05 0 0 0.06 0.07 0.11 0.09 0.12 0.1
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.06 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0.07 0.19 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0.71 0.54 0.58 0.55 0.57 0.54 0.56 – 0.47 0.47 0.42 0.52 0.38 0.36 0.28 0.65 0.62 0.58 0.64 0.64 0.64 0.49 0.46 0.49
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0.05 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 10 5 2 2 8 – 2 6 0 1 1 0 0 7 1 4 1 0 0 0 5 3
Bud 1 8 5 9 1 11 22 – 24 21 29 15 1 0 1 10 3 3 1 0 0 0 6 3
Bud Neck 8 13 57 44 46 34 69 – 44 27 31 37 1 1 0 6 10 9 0 2 0 0 3 5
Bud Site 2 0 2 4 1 5 11 – 15 18 16 4 1 0 0 5 2 1 – – – – – –
Cell Periphery 0 0 1 0 1 1 0 – 0 0 0 1 0 0 0 1 1 0 0 0 0 0 1 0
Cytoplasm 29 69 97 106 67 115 118 – 89 85 94 87 50 63 62 91 69 72 16 34 33 13 45 24
Endoplasmic Reticulum 0 4 1 1 0 0 0 – 4 0 0 2 1 0 3 16 8 17 0 0 0 0 1 0
Endosome 13 22 0 1 0 9 2 – 8 2 7 5 33 91 58 115 128 62 25 37 19 4 14 15
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 1 1 0 0 0 0 1 0
Mitochondria 3 32 0 2 2 26 24 – 116 109 123 52 13 2 5 28 5 9 11 22 32 5 27 16
Nucleus 3 2 1 3 1 2 2 – 2 3 0 1 1 1 2 9 9 7 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 2 1 0 1 0
Nucleolus 3 1 0 0 0 2 1 – 2 11 4 2 4 2 2 37 26 16 2 8 5 0 1 0
Peroxisomes 2 0 0 0 0 1 0 – 1 0 6 0 10 35 8 8 0 4 0 1 0 0 1 0
SpindlePole 105 139 165 149 119 178 203 – 174 154 149 150 55 67 43 344 251 171 118 218 193 29 103 75
Vac/Vac Membrane 4 4 1 0 0 2 0 – 1 2 3 0 7 4 2 27 28 14 2 5 2 1 4 6
Unique Cell Count 148 259 286 269 210 328 364 369 329 351 286 143 186 151 533 405 295 185 344 303 61 223 156
Labelled Cell Count 175 297 340 324 240 388 460 482 438 462 358 178 266 186 706 542 390 185 344 303 61 223 156


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.8 4.9 4.4 5.1 3.9 4.0 – 3.3 3.3 3.2 3.7 4.0 4.3 4.3 6.2 6.6 6.2 3.7 4.0 4.3
Std Deviation (1e-4) 0.6 0.5 1.0 1.4 1.4 1.4 1.1 – 0.6 0.6 0.6 0.7 0.9 1.6 1.2 1.2 2.6 1.7 1.3 1.2 1.3
Intensity Change (Log2) – – – -0.15 0.05 -0.32 -0.29 – -0.55 -0.56 -0.59 -0.41 -0.28 -0.17 -0.18 0.34 0.43 0.34 -0.41 -0.3 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.5 4.2 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0094 -0.7387 -0.7113 -0.5964 -0.8678 -0.7759 1.0243 0.003 -0.1334 0.0714 0.3976 0.5168 0.0403 -0.7013 -0.7301 -0.8382 -1.0839 -0.7022
Actin 0.0014 0.0007 0.0084 0.0016 0.0114 0.0028 0.0699 0.0008 0.0086 0.0013 0.023 0.0175 0.0795 0.0008 0.0309 0.0004 0.0001 0.0026
Bud 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0013 0.0019 0.0006 0.0001 0.0012 0.0004 0.0025 0.0009 0.0005 0.0002 0 0.0003
Bud Neck 0.0017 0.0008 0.0015 0.0109 0.0008 0.0035 0.0033 0.0019 0.0011 0.0078 0.0109 0.0013 0.0024 0.0011 0.0006 0.0003 0.0002 0.0004
Bud Periphery 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0023 0.0009 0.0007 0.0002 0.0026 0.0008 0.0076 0.0014 0.0005 0.0004 0 0.0004
Bud Site 0.0004 0.0037 0.0009 0.0009 0.0001 0.0005 0.0066 0.0133 0.0029 0.0007 0.0079 0.0002 0.0065 0.0062 0.0036 0.0001 0 0.0001
Cell Periphery 0 0.0001 0.0001 0.0001 0 0.0001 0.0004 0.0002 0.0001 0.0001 0.0015 0.0001 0.0004 0.0001 0.0001 0 0 0
Cytoplasm 0.0311 0.035 0.0277 0.0344 0.0138 0.039 0.0456 0.0613 0.0402 0.0535 0.0211 0.0702 0.028 0.0615 0.0264 0.0178 0.0015 0.0406
Cytoplasmic Foci 0.1671 0.1127 0.1393 0.1693 0.1821 0.1884 0.1559 0.1003 0.1058 0.1121 0.2678 0.1118 0.2617 0.1292 0.189 0.0828 0.41 0.1605
Eisosomes 0.0003 0.0003 0.0003 0.0001 0.0001 0.0001 0.0006 0.0001 0.0001 0.0001 0.0003 0.0002 0.0005 0.0002 0.0002 0.0001 0 0.0001
Endoplasmic Reticulum 0.0013 0.0013 0.0032 0.0009 0.0026 0.0012 0.0031 0.0019 0.0014 0.0005 0.005 0.0026 0.0106 0.002 0.001 0.0006 0.0001 0.0017
Endosome 0.0114 0.0119 0.0096 0.014 0.0216 0.0091 0.0387 0.0135 0.0074 0.0244 0.0507 0.0175 0.0263 0.017 0.0177 0.004 0.0322 0.0202
Golgi 0.0012 0.0011 0.002 0.0014 0.0015 0.0012 0.0152 0.0013 0.002 0.0192 0.0153 0.0039 0.0077 0.0016 0.0064 0.0005 0.0036 0.0048
Lipid Particles 0.0279 0.0219 0.0279 0.0293 0.0773 0.0194 0.0455 0.0137 0.013 0.0222 0.1382 0.0117 0.0327 0.0128 0.0118 0.0042 0.1701 0.011
Mitochondria 0.0004 0.0007 0.0006 0.0013 0.0007 0.0006 0.0108 0.0008 0.0008 0.0036 0.0045 0.002 0.0108 0.0009 0.0027 0.001 0.0001 0.0051
None 0.3384 0.5854 0.3713 0.4253 0.339 0.3206 0.3056 0.5002 0.3465 0.3569 0.1443 0.3761 0.2711 0.5756 0.3727 0.5426 0.1658 0.3115
Nuclear Periphery 0.0091 0.0036 0.0072 0.0054 0.0249 0.0062 0.0263 0.0034 0.0043 0.0014 0.0182 0.0159 0.0197 0.0037 0.0027 0.0148 0.0004 0.0139
Nucleolus 0.0042 0.0052 0.0067 0.0089 0.0022 0.0062 0.0061 0.0103 0.0056 0.0039 0.0051 0.0018 0.0016 0.0042 0.0016 0.0052 0.0009 0.0017
Nucleus 0.0253 0.0143 0.0155 0.033 0.012 0.0135 0.0259 0.0228 0.0208 0.0227 0.0098 0.0222 0.007 0.0111 0.0032 0.0238 0.0003 0.004
Peroxisomes 0.0078 0.0049 0.0149 0.0129 0.0197 0.0158 0.0351 0.0054 0.0089 0.0081 0.0591 0.0064 0.0262 0.005 0.0138 0.007 0.046 0.0044
Punctate Nuclear 0.3685 0.1936 0.3614 0.2476 0.2873 0.3683 0.1935 0.2431 0.4283 0.3585 0.1915 0.3357 0.191 0.162 0.3127 0.2909 0.1684 0.4128
Vacuole 0.0021 0.0019 0.0008 0.0018 0.0017 0.0028 0.0056 0.0029 0.0008 0.0023 0.0196 0.0008 0.0039 0.0023 0.0013 0.0016 0.0002 0.0016
Vacuole Periphery 0.0004 0.0003 0.0003 0.0004 0.0006 0.0003 0.0028 0.0003 0.0002 0.0005 0.0023 0.0006 0.0022 0.0006 0.0005 0.0017 0 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.7778 5.3753 3.6563 5.6724 6.172 6.5106 5.435 5.3496 4.7072 11.3489
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
602 1349 2352 847 2055 2122 2307 173 2657 3471 4659 1020

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 581.33 658.07 898.87 781.65 667.54 699.60 739.46 789.39 648.01 683.46 819.93 782.96
Standard Deviation 58.74 104.78 110.21 101.60 70.39 85.61 86.26 89.24 76.92 95.69 127.15 99.65
Intensity Change Log 2 — 0.178884 0.628755 0.427165 — 0.067676 0.147618 0.241884 — 0.120510 0.391603 0.331097

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000076 0.007992 0.000424 0.003621 0.000149 0.000372 0.000112 0.000645 0.000132 0.003333 0.000270 0.003116
Bud Neck 0.020201 0.022193 0.036719 0.014394 0.012161 0.023473 0.015251 0.007240 0.013983 0.022975 0.026089 0.013181
Bud Site 0.002671 0.020339 0.023006 0.089071 0.001649 0.010633 0.008179 0.075873 0.001880 0.014405 0.015664 0.086833
Cell Periphery 0.000186 0.000465 0.000114 0.000275 0.000106 0.000122 0.000445 0.000197 0.000124 0.000256 0.000278 0.000261
Cytoplasm 0.283304* 0.197631* 0.122233* 0.336114* 0.240324* 0.205974* 0.386732* 0.418002* 0.250062* 0.202731* 0.253205* 0.350003*
Cytoplasmic Foci 0.449207* 0.341614* 0.001799 0.019925 0.402325* 0.358133* 0.036110 0.025885 0.412947* 0.351713* 0.018789 0.020936
Eisosomes 0.000271 0.000337 0.000018 0.000058 0.000136 0.000106 0.000212 0.000083 0.000167 0.000195 0.000114 0.000062
Endoplasmic Reticulum 0.000312 0.001411 0.002101 0.003252 0.000566 0.000465 0.000935 0.001459 0.000508 0.000832 0.001524 0.002948
Endosome 0.016910 0.033612 0.001775 0.012619 0.020064 0.033631 0.004355 0.006175 0.019349 0.033624 0.003053 0.011526
Golgi 0.005550 0.019234 0.000238 0.011113 0.006705 0.014315 0.000350 0.000203 0.006443 0.016227 0.000293 0.009262
Lipid Particles 0.049595 0.039226 0.000467 0.001779 0.078422 0.040379 0.002666 0.002891 0.071890 0.039931 0.001555 0.001968
Mitochondria 0.012793 0.016537 0.003406 0.011137 0.009619 0.024865 0.003279 0.000740 0.010338 0.021628 0.003343 0.009374
Mitotic Spindle 0.016671 0.123927* 0.408497* 0.121433* 0.053881 0.131122* 0.096156 0.061361 0.045451 0.128325* 0.253835* 0.111244*
None 0.013289 0.012785 0.012780 0.007035 0.012025 0.006712 0.015900 0.010844 0.012312 0.009072 0.014325 0.007681
Nuclear Periphery 0.000378 0.001086 0.002855 0.002151 0.001480 0.001089 0.001506 0.001422 0.001230 0.001088 0.002187 0.002027
Nuclear Periphery Foci 0.002592 0.005213 0.002339 0.003162 0.007414 0.003446 0.001718 0.001583 0.006321 0.004132 0.002032 0.002895
Nucleolus 0.012099 0.011289 0.019733 0.001486 0.027809 0.014707 0.007967 0.002047 0.024250 0.013379 0.013907 0.001581
Nucleus 0.082850 0.059836 0.349333* 0.239355* 0.090591 0.063510 0.390296* 0.274589* 0.088837 0.062082 0.369616* 0.245331*
Peroxisomes 0.020541 0.061655 0.000334 0.003435 0.025608 0.043168 0.001920 0.000831 0.024460 0.050353 0.001120 0.002993
Vacuole 0.009835 0.022080 0.011127 0.106114 0.008399 0.021556 0.025471 0.107556 0.008724 0.021760 0.018230 0.106359
Vacuole Periphery 0.000669 0.001540 0.000701 0.012472 0.000568 0.002222 0.000440 0.000374 0.000591 0.001957 0.000572 0.010420

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.49 -17.73 -4.36 2.89 -2.96 -3.91 -7.08 -4.74 -3.71 -3.68 -4.73 -13.60 -4.80 1.07 -3.63
Bud Neck -1.02 -6.26 -0.14 1.16 8.26 -8.22 -8.70 -0.95 2.11 2.11 -7.33 -13.59 -3.25 2.60 7.03
Bud Site -7.56 -8.64 -7.84 -4.40 -5.03 -7.25 -10.24 -3.37 -2.64 -2.48 -10.55 -12.98 -8.65 -6.10 -6.01
Cell Periphery -2.62 6.09 -2.15 2.11 -10.96 -2.12 -5.91 -5.35 -4.06 1.35 -3.40 -3.75 -8.79 0.18 -2.72
Cytoplasm 3.10 13.60 -4.40 -9.11 -23.22 -2.92 -12.90 -6.39 -5.43 -1.95 -1.12 1.70 -11.54 -11.07 -13.33
Cytoplasmic Foci 11.70 42.99 40.92 43.13 -9.20 6.67 58.49 53.02 47.72 3.06 11.94 75.36 73.66 70.19 0.53
Eisosomes -0.91 6.48 3.72 4.55 -14.40 1.25 -1.47 -2.71 -3.36 -0.76 -1.73 3.33 1.44 3.01 -2.60
Endoplasmic Reticulum -2.36 -16.59 -17.98 -10.32 -10.23 -1.10 -11.72 -7.93 -7.84 -5.11 -2.42 -19.85 -19.49 -16.44 -12.23
Endosome -6.86 7.93 5.20 15.15 -5.91 -6.87 13.37 8.04 12.85 -0.33 -9.46 18.33 13.51 22.13 -2.39
Golgi -6.58 10.22 3.90 8.60 -1.93 -6.59 14.21 14.81 12.94 3.31 -9.49 17.32 4.84 10.92 -1.84
Lipid Particles 3.46 11.91 11.27 13.05 -13.20 7.57 22.25 21.24 16.22 -2.10 8.70 25.53 24.78 21.70 -7.13
Mitochondria -1.12 4.07 3.11 4.73 -0.51 -8.20 4.67 7.47 13.35 5.06 -7.60 6.18 3.52 9.70 -0.31
Mitotic Spindle -16.24* -41.06* -8.38 6.15 26.43* -8.75 -16.69 -1.16 1.94 5.64 -14.57 -36.90 -3.57 6.32 22.86*
None 1.92 0.67 3.20 1.90 7.22 5.14 -1.04 3.15 -0.15 3.80 4.14 -1.10 4.65 0.79 7.02
Nuclear Periphery -4.33 -25.05 -19.07 -14.46 -5.12 2.82 -14.26 -5.50 -6.54 1.65 1.28 -21.83 -16.21 -16.61 -4.51
Nuclear Periphery Foci -2.55 1.42 0.76 3.40 -0.53 6.89 6.32 2.32 -0.75 -0.40 5.17 7.59 5.97 1.84 0.24
Nucleolus 3.22 -15.65 7.50 8.25 29.38* 12.38* 3.78 19.97 10.82 16.83 13.48* -6.95 22.13 14.01 32.95*
Nucleus 1.45 -28.00 -22.90 -24.96 -0.44 0.67 -35.49 -10.27 -10.39 0.62 1.32 -45.84 -26.87 -27.53 -0.06
Peroxisomes -8.42 15.82 14.60 19.94 -2.87 -9.08 22.27 22.58 25.16 3.41 -12.62 28.71 27.15 31.78 1.04
Vacuole -5.90 -1.56 -23.94 -22.46 -23.87 -9.40 -15.45 -10.18 -9.57 -8.53 -11.98 -14.96 -26.49 -24.82 -24.16
Vacuole Periphery -2.35 2.77 -1.99 -1.35 -2.19 -5.09 0.01 -0.68 4.26 -0.67 -5.66 1.45 -2.11 -0.92 -2.19
Standard name
Human Ortholog
Description Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6
Localization
Cell Percentages spindle pole (20%), nucleolus (4%), mixed (35%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ctf3

Ctf3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ctf3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available