Standard name
Human Ortholog
Description Mitochondrial leucyl-tRNA synthetase; also has direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance; human homolog LARS2 can complement yeast null mutant, and is implicated in Perrault syndrome
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.08 0 0 0 0 0 0 – 0 0 0.05 0 0 0.12 0 0.1 0 0 0 0 0 0
Bud 0.05 0 0.11 0 0.06 0 0.06 0 0.06 – 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.12 0.09 0.09 0 0 0 0 0 0
Cytoplasm 0.06 0 0.15 0.06 0.08 0.1 0 0.05 0.05 – 0 0 0.08 0.15 0.23 0.14 0.11 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Endosome 0 0 0.07 0 0.1 0 0.06 0.05 0 – 0 0 0 0.19 0.18 0.08 0.11 0.09 0.1 0.09 0.1 0.07 0.09 0.08
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.15 0.21 0.14 0 0 0 0 0 0
Mitochondria 0.86 0.87 0.49 0.85 0.71 0.81 0.75 0.85 0.93 – 0.94 0.89 0.84 0.56 0.55 0.16 0.14 0.15 0.71 0.72 0.74 0.69 0.61 0.64
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.05 0.05 0.06 0.09 0 0 0 0 – 0 0 0 0.1 0.08 0.07 0.07 0 0 0.05 0 0.07 0.09 0.06
SpindlePole 0.05 0 0.18 0.06 0.1 0.06 0.12 0.12 0 – 0.06 0.1 0 0 0 0.18 0.21 0.27 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.13 0.06 0 0.12 0.08 0.1 0.11 0.06 – 0.05 0.07 0.08 0.08 0.06 0.1 0.1 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 6 12 4 1 0 2 2 0 – 0 1 14 2 1 38 9 20 13 7 1 5 11 19
Bud 23 5 16 3 15 6 16 12 15 – 7 17 7 7 4 10 5 6 2 2 0 5 6 6
Bud Neck 4 0 5 0 1 1 1 0 0 – 0 0 0 0 0 2 0 0 4 1 0 5 14 11
Bud Site 5 1 0 0 0 0 4 4 4 – 0 3 0 0 0 0 4 1 – – – – – –
Cell Periphery 8 11 4 2 5 0 6 1 3 – 2 3 7 12 3 39 19 19 0 0 0 1 1 2
Cytoplasm 27 8 22 8 20 21 11 15 12 – 5 6 22 42 74 43 23 21 2 1 1 0 1 1
Endoplasmic Reticulum 7 10 5 3 3 0 0 1 2 – 2 1 8 4 5 19 9 13 3 0 2 4 4 7
Endosome 15 6 11 6 24 7 15 15 0 – 4 3 9 53 57 24 23 19 53 32 20 19 39 38
Golgi 2 1 1 1 1 1 0 2 0 – 0 0 5 0 3 46 43 28 15 8 3 5 8 6
Mitochondria 413 267 73 118 172 179 187 257 232 – 144 177 245 155 178 51 28 30 381 251 156 182 260 297
Nucleus 7 1 1 1 1 8 11 10 5 – 3 2 0 1 0 11 11 4 3 0 0 0 0 2
Nuclear Periphery 3 3 0 0 2 2 4 4 6 – 3 6 2 1 1 0 1 1 3 3 2 0 3 5
Nucleolus 5 1 0 0 0 2 4 5 11 – 4 2 0 0 0 1 1 1 1 2 0 1 2 2
Peroxisomes 9 14 7 9 21 7 10 7 0 – 3 4 12 28 26 22 14 6 18 16 8 17 39 27
SpindlePole 23 7 27 9 25 13 31 38 8 – 9 19 1 8 4 58 43 56 8 2 1 1 5 5
Vac/Vac Membrane 38 39 9 4 29 17 26 32 15 – 7 14 22 23 18 33 21 22 17 11 4 9 17 19
Unique Cell Count 480 307 149 139 242 221 249 304 250 154 200 293 279 324 315 204 204 535 348 211 266 424 464
Labelled Cell Count 594 380 193 168 320 264 328 405 313 193 258 354 336 374 397 254 247 535 348 211 266 424 464


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.8 5.7 5.1 5.8 4.8 5.6 5.1 4.4 – 4.3 4.7 5.0 5.3 5.2 8.0 8.0 7.6 5.0 5.1 5.2
Std Deviation (1e-4) 0.8 0.7 2.2 2.2 1.4 1.4 1.9 1.0 1.3 – 1.2 1.5 0.9 1.2 1.5 1.5 1.6 1.5 1.7 1.7 1.4
Intensity Change (Log2) – – – -0.17 0.0 -0.24 -0.05 -0.16 -0.38 – -0.43 -0.29 -0.21 -0.12 -0.15 0.49 0.49 0.41 -0.2 -0.18 -0.14

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.6 1.4 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9714 3.6308 3.3582 3.182 3.2203 3.4155 1.0834 1.7884 1.3328 1.0945 1.2555 1.43 1.4104 1.9635 1.7584 1.6213 1.7975 1.7179
Actin 0.0248 0.0092 0.0094 0.0013 0.0352 0.0018 0.0312 0.0467 0.0247 0.0186 0.0145 0.0259 0.0193 0.0293 0.029 0.006 0.0508 0.0291
Bud 0.0004 0.0035 0.0012 0.0002 0.0004 0.0005 0.0011 0.0031 0.001 0.0011 0.0043 0.0006 0.0012 0.003 0.0046 0.0007 0.007 0.0034
Bud Neck 0.0009 0.0008 0.0076 0.0005 0.0003 0.0011 0.0012 0.0014 0.0012 0.0002 0.001 0.0017 0.0034 0.0019 0.0031 0.0002 0.0037 0.0042
Bud Periphery 0.0014 0.0078 0.0044 0.0009 0.0009 0.003 0.0015 0.0053 0.0019 0.0074 0.0106 0.0012 0.0019 0.0032 0.0062 0.0019 0.0178 0.0092
Bud Site 0.005 0.0133 0.0086 0.0005 0.0015 0.0005 0.0035 0.0224 0.0056 0.0007 0.0087 0.0009 0.01 0.0376 0.0313 0.0006 0.0096 0.003
Cell Periphery 0.0015 0.0006 0.004 0.0018 0.0002 0.0046 0.0007 0.0012 0.0009 0.0009 0.0005 0.0003 0.0009 0.0009 0.0014 0.0004 0.0008 0.0008
Cytoplasm 0.0063 0.0047 0.0021 0.0063 0.0007 0.003 0.0077 0.0092 0.0044 0.0005 0.0009 0.0048 0.0056 0.0063 0.011 0.0018 0.0037 0.0074
Cytoplasmic Foci 0.0276 0.0619 0.0102 0.0153 0.0098 0.0342 0.0596 0.0563 0.0396 0.0081 0.0278 0.0336 0.0832 0.0714 0.0885 0.0131 0.0866 0.0809
Eisosomes 0.0021 0.0022 0.0008 0.001 0.0004 0.0003 0.0006 0.0008 0.0004 0.0005 0.0003 0.0004 0.0007 0.0006 0.0009 0.0003 0.0006 0.0004
Endoplasmic Reticulum 0.0074 0.0005 0.0003 0.0121 0.0011 0.001 0.0052 0.0007 0.0026 0.0001 0.0009 0.0008 0.0032 0.0013 0.0017 0.0025 0.0026 0.0013
Endosome 0.0473 0.0523 0.0262 0.0601 0.0143 0.0152 0.1023 0.0604 0.0543 0.0162 0.0709 0.0431 0.0677 0.0576 0.0467 0.0472 0.0582 0.0427
Golgi 0.0374 0.0787 0.0187 0.0458 0.0122 0.0184 0.0846 0.0673 0.0537 0.0236 0.1141 0.08 0.0444 0.0626 0.0516 0.0198 0.033 0.0517
Lipid Particles 0.0527 0.0336 0.0162 0.0296 0.0114 0.0291 0.0312 0.0332 0.0104 0.0023 0.0527 0.0196 0.1133 0.0213 0.0331 0.0337 0.0508 0.0606
Mitochondria 0.6717 0.6255 0.8365 0.7251 0.8475 0.8238 0.5821 0.6059 0.7238 0.8887 0.6504 0.7331 0.5158 0.5698 0.5601 0.8303 0.5309 0.6009
None 0.0164 0.0058 0.0102 0.0365 0.0025 0.0035 0.0068 0.007 0.0012 0.0003 0.0004 0.0055 0.0232 0.0076 0.0206 0.0019 0.0201 0.0139
Nuclear Periphery 0.0062 0.0003 0.0002 0.0023 0.0016 0.0002 0.0032 0.0004 0.0029 0 0.0006 0.0004 0.0025 0.0004 0.0034 0.0104 0.0147 0.0011
Nucleolus 0.0038 0.0007 0.0011 0.0004 0.0002 0.0004 0.0049 0.0005 0.0007 0 0.0038 0.0005 0.0022 0.0018 0.0033 0.0007 0.0027 0.001
Nucleus 0.0022 0.0003 0.0005 0.0005 0.0003 0.0003 0.0011 0.0007 0.0006 0.0001 0.0006 0.0002 0.0013 0.0007 0.0019 0.0012 0.018 0.001
Peroxisomes 0.0712 0.0921 0.0311 0.0496 0.0553 0.0536 0.0583 0.0704 0.061 0.0291 0.0301 0.0436 0.0854 0.1126 0.0904 0.0161 0.0725 0.0779
Punctate Nuclear 0.001 0.0006 0.0005 0.0002 0.0024 0.0009 0.0019 0.0008 0.0004 0 0.0005 0.0004 0.0028 0.0011 0.004 0.0022 0.0071 0.0035
Vacuole 0.0081 0.0034 0.0054 0.0052 0.0007 0.0023 0.0077 0.0053 0.0057 0.0006 0.0036 0.0015 0.0074 0.0058 0.0045 0.0042 0.0041 0.0034
Vacuole Periphery 0.0045 0.0021 0.0047 0.0048 0.0011 0.0022 0.0039 0.0015 0.0028 0.0009 0.0029 0.002 0.0044 0.0033 0.0026 0.0049 0.0046 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.7942 7.7147 12.267 13.4759 11.9884 13.1432 12.9076 13.0363 15.3109 19.4012
Translational Efficiency 0.4262 0.5593 0.4014 0.4917 0.4367 0.6199 0.5218 0.4263 0.4984 0.3998

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2133 898 211 750 2054 2612 1490 167 4187 3510 1701 917

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 691.49 797.55 1120.09 909.35 712.95 764.25 911.20 948.07 702.02 772.77 937.11 916.40
Standard Deviation 75.62 109.34 147.87 128.48 107.72 142.06 116.03 141.51 93.39 135.23 138.73 131.80
Intensity Change Log 2 — 0.205867 0.695834 0.395127 — 0.100244 0.353967 0.411193 — 0.153215 0.532401 0.403305

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000612 0.002471 0.006562 0.026308 0.000601 0.001072 0.004628 0.030681 0.000607 0.001430 0.004868 0.027104
Bud Neck 0.012347 0.005909 0.010090 0.017963 0.007119 0.004494 0.006636 0.005862 0.009783 0.004856 0.007065 0.015759
Bud Site 0.012005 0.013834 0.037897 0.159234 0.008376 0.008678 0.027862 0.115761 0.010225 0.009997 0.029107 0.151317
Cell Periphery 0.000320 0.000382 0.000312 0.000480 0.000406 0.000206 0.000229 0.000593 0.000362 0.000251 0.000240 0.000501
Cytoplasm 0.013288 0.007543 0.002223 0.046463 0.005275 0.006775 0.007712 0.026137 0.009358 0.006972 0.007031 0.042761
Cytoplasmic Foci 0.067291 0.066188 0.108164 0.105913 0.043661 0.057045 0.086512 0.133212 0.055699 0.059384 0.089198 0.110884
Eisosomes 0.000092 0.000108 0.000092 0.000174 0.000095 0.000085 0.000098 0.000209 0.000093 0.000091 0.000097 0.000180
Endoplasmic Reticulum 0.001241 0.000448 0.001969 0.002681 0.000350 0.000209 0.002369 0.001419 0.000804 0.000270 0.002319 0.002451
Endosome 0.087422 0.105047 0.116415 0.071271 0.072419 0.086140 0.117776 0.091636 0.080062 0.090977 0.117607 0.074980
Golgi 0.085418 0.152883 0.089620 0.099401 0.122835 0.162223 0.102772 0.200180 0.103774 0.159834 0.101140 0.117754
Lipid Particles 0.005320 0.004402 0.007680 0.001660 0.006986 0.002777 0.003523 0.001701 0.006138 0.003193 0.004039 0.001668
Mitochondria 0.620263 0.540041 0.258310 0.170608 0.668062 0.599505 0.370886 0.187471 0.643712 0.584291 0.356921 0.173679
Mitotic Spindle 0.006111 0.015338 0.139147 0.087698 0.006386 0.010840 0.107858 0.045839 0.006246 0.011991 0.111739 0.080075
None 0.001022 0.000260 0.000234 0.000258 0.001055 0.000941 0.000282 0.000163 0.001038 0.000766 0.000276 0.000241
Nuclear Periphery 0.000228 0.000082 0.003164 0.000156 0.000082 0.000083 0.001042 0.000059 0.000157 0.000082 0.001305 0.000138
Nuclear Periphery Foci 0.000170 0.000381 0.006512 0.000436 0.000164 0.000172 0.001720 0.000227 0.000167 0.000225 0.002314 0.000398
Nucleolus 0.001038 0.000725 0.001281 0.000604 0.001703 0.000423 0.001407 0.000111 0.001364 0.000500 0.001392 0.000514
Nucleus 0.001969 0.000773 0.001951 0.004578 0.001824 0.001752 0.004186 0.000436 0.001898 0.001502 0.003909 0.003824
Peroxisomes 0.016749 0.058172 0.061804 0.123992 0.031290 0.032582 0.080186 0.102586 0.023882 0.039129 0.077906 0.120093
Vacuole 0.036735 0.012190 0.082081 0.066759 0.009060 0.012686 0.050553 0.044229 0.023159 0.012559 0.054464 0.062656
Vacuole Periphery 0.030357 0.012822 0.064492 0.013365 0.012248 0.011312 0.021764 0.011487 0.021473 0.011698 0.027064 0.013023

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.11 -2.69 -7.39 -6.64 -4.70 -2.61 -6.01 -5.27 -5.19 -4.52 -2.98 -6.52 -8.79 -8.48 -7.15
Bud Neck 4.52 1.19 -3.00 -6.41 -3.62 2.35 0.20 0.71 -1.04 0.64 5.65 2.77 -3.98 -7.47 -5.80
Bud Site -0.94 -3.55 -19.28 -18.72 -11.54 -0.26 -10.21 -6.92 -6.90 -5.61 0.24 -10.29 -20.57 -20.59 -17.24
Cell Periphery -0.60 0.10 -1.47 -1.09 -2.46 2.78 2.51 -1.22 -2.84 -2.69 1.89 2.18 -1.73 -4.76 -5.29
Cytoplasm 3.15 7.38 -10.17 -11.33 -13.10 -1.44 -2.69 -3.81 -3.52 -3.22 2.37 1.45 -11.75 -12.46 -12.12
Cytoplasmic Foci 0.24 -3.65 -7.05 -6.72 0.24 -5.19 -12.36 -7.91 -6.73 -4.07 -1.69 -10.18 -11.68 -10.86 -4.00
Eisosomes -1.30 -0.09 -5.63 -4.98 -6.27 0.45 -0.19 -3.49 -4.86 -4.35 0.16 -0.42 -5.78 -8.65 -8.23
Endoplasmic Reticulum 2.34 -2.09 -1.75 -2.84 -0.82 1.95 -5.83 -3.35 -3.85 2.17 3.15 -4.55 -2.51 -3.40 -0.12
Endosome -3.08 -3.11 3.89 5.87 5.00 -3.62 -9.30 -2.07 -0.60 2.64 -3.55 -8.93 1.78 4.32 8.77
Golgi -9.34 -0.35 -1.28 6.87 -0.45 -6.92 3.77 -4.41 -2.13 -5.67 -13.26 0.86 -1.44 7.14 -1.91
Lipid Particles 0.60 -1.12 4.91 1.67 2.79 3.55 2.97 3.80 1.07 2.02 3.72 2.82 5.95 2.06 3.98
Mitochondria 5.79 15.72 38.37 24.50 3.94 6.77 25.41 21.70 18.88 7.93 7.62 29.32 46.82 39.63 15.36
Mitotic Spindle -4.59 -8.55 -13.27 -11.33 2.97 -3.91 -19.54 -4.37 -3.87 5.98 -6.01 -21.40 -13.89 -12.72 4.22
None 2.85 3.04 2.91 0.04 -0.85 0.29 3.23 3.76 2.28 3.08 0.86 4.26 4.50 2.06 1.15
Nuclear Periphery 2.07 -3.47 0.47 -2.89 3.52 -0.03 -4.19 1.31 0.64 4.29 1.70 -5.02 -0.31 -2.39 4.99
Nuclear Periphery Foci -0.92 -1.99 -3.42 -0.56 1.88 -0.12 -4.81 -1.17 -0.64 4.53 -0.71 -4.42 -3.61 -2.09 3.76
Nucleolus 0.77 -0.57 0.59 -0.18 1.01 2.35 0.33 3.26 1.90 4.14 2.63 -0.30 1.90 -0.50 2.10
Nucleus 2.93 -0.54 -4.14 -5.20 -3.43 0.06 -2.98 2.73 2.29 5.11 0.70 -3.20 -3.85 -4.01 -1.01
Peroxisomes -8.51 -4.68 -13.71 -7.05 -4.85 -0.43 -9.97 -4.78 -4.71 -1.43 -6.59 -12.83 -13.78 -11.28 -5.02
Vacuole 11.88 -5.19 -7.76 -12.32 0.37 -3.51 -15.17 -4.76 -4.27 0.89 9.12 -11.71 -10.75 -13.08 -3.22
Vacuole Periphery 9.66 -4.14 8.15 0.03 6.25 0.95 -6.03 0.26 -0.05 3.13 10.21 -3.05 5.29 -0.51 6.59
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Mitochondrial leucyl-tRNA synthetase; also has direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance; human homolog LARS2 can complement yeast null mutant, and is implicated in Perrault syndrome
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Nam2

Nam2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nam2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available