Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 1 | 3 | 3 | 10 | 2 | 4 | 2 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 0 | 10 | 4 | 4 | 8 | 3 | 10 | 6 | 15 | 8 | 22 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 12 | 19 | 2 | 10 | 15 | 13 | 11 | 18 | 24 | 25 | 24 | 4 | 12 | 9 | 17 | 24 | 18 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 187 | 458 | 419 | 230 | 324 | 324 | 402 | 361 | 316 | 323 | 246 | 341 | 156 | 178 | 172 | 295 | 261 | 246 | 193 | 452 | 433 | 108 | 178 | 184 |
Endoplasmic Reticulum | 5 | 16 | 14 | 0 | 5 | 4 | 8 | 15 | 13 | 15 | 10 | 5 | 1 | 0 | 0 | 14 | 13 | 17 | 6 | 12 | 14 | 7 | 14 | 21 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 0 | 0 | 2 | 1 | 0 | 0 | 0 |
Mitochondria | 0 | 2 | 8 | 25 | 33 | 76 | 124 | 141 | 146 | 159 | 128 | 288 | 0 | 1 | 9 | 4 | 2 | 3 | 3 | 4 | 7 | 0 | 1 | 1 |
Nucleus | 0 | 1 | 0 | 0 | 2 | 4 | 6 | 6 | 0 | 2 | 4 | 3 | 1 | 0 | 0 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 2 | 7 | 1 | 3 | 1 | 23 | 48 | 7 | 8 | 9 | 41 | 0 | 0 | 0 | 18 | 17 | 7 | 2 | 9 | 15 | 3 | 1 | 2 |
Unique Cell Count | 196 | 475 | 447 | 239 | 352 | 370 | 510 | 512 | 425 | 459 | 350 | 547 | 157 | 178 | 176 | 321 | 287 | 259 | 212 | 493 | 479 | 120 | 198 | 215 |
Labelled Cell Count | 203 | 492 | 478 | 262 | 383 | 435 | 582 | 604 | 508 | 551 | 433 | 731 | 164 | 191 | 192 | 358 | 324 | 291 | 212 | 493 | 479 | 120 | 198 | 215 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.1 | 15.5 | 8.1 | 7.5 | 7.3 | 6.3 | 5.9 | 5.8 | 5.3 | 5.4 | 5.1 | 4.8 | 12.7 | 11.6 | 9.7 | 19.4 | 21.0 | 20.7 | 16.1 | 16.7 | 17.1 |
Std Deviation (1e-4) | 3.3 | 2.8 | 1.2 | 1.2 | 1.3 | 1.2 | 1.1 | 1.1 | 1.1 | 1.2 | 1.2 | 1.1 | 1.8 | 1.6 | 1.4 | 4.8 | 5.2 | 5.1 | 3.1 | 3.2 | 3.0 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.15 | -0.37 | -0.47 | -0.49 | -0.63 | -0.58 | -0.68 | -0.76 | 0.64 | 0.51 | 0.26 | 1.25 | 1.37 | 1.35 | 0.99 | 1.04 | 1.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -1.2 | -0.1 | -2.2 | -0.3 | -0.9 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.5 | -1.1 | -0.1 | -1.5 | -1.9 | 0 | 0.7 | 1.8 | 0 | -1.0 | 1.3 | 0.5 | 0.7 | 2.3 | 1.6 |
Cytoplasm | 1.4 | -0.9 | -3.1 | -6.6 | -9.2 | -7.9 | -9.1 | -8.8 | -11.6 | 2.8 | 3.4 | 2.0 | -1.0 | -1.4 | 0.7 |
Endoplasmic Reticulum | 0 | -1.6 | -2.0 | -1.6 | -0.2 | -0.1 | 0.1 | -0.2 | 0 | 0 | 0 | 0 | 0.9 | 1.0 | 2.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.1 | 4.8 | 8.8 | 10.1 | 11.0 | 12.6 | 12.7 | 13.0 | 0 | 0 | 0 | 0 | -0.6 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.6 | 5.2 | 0.1 | 0.2 | 1.0 | 4.3 | 0 | 0 | 0 | 3.1 | 3.2 | 1.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.775 | 17.0759 | 14.7399 | 13.8871 | 13.4868 | 15.0085 | 12.6242 | 17.0921 | 15.4705 | 13.4668 | 14.859 | 16.1053 | 15.1875 | 18.4086 | 17.2862 | 16.2635 | 16.3383 | 16.9735 |
Actin | 0.0014 | 0.0012 | 0.0007 | 0.0008 | 0.0005 | 0.0013 | 0.0244 | 0.0003 | 0.015 | 0.0056 | 0.0021 | 0.0036 | 0.0102 | 0.0012 | 0.0007 | 0.0007 | 0.0036 | 0.0008 |
Bud | 0.0007 | 0.0007 | 0.0013 | 0.0012 | 0.0006 | 0.001 | 0.001 | 0.0002 | 0.0015 | 0.0011 | 0.0004 | 0.0004 | 0.0057 | 0.0057 | 0.001 | 0.0005 | 0.0123 | 0.0008 |
Bud Neck | 0.001 | 0.0008 | 0.001 | 0.0014 | 0.0016 | 0.0048 | 0.0013 | 0.0003 | 0.0018 | 0.0014 | 0.0005 | 0.0015 | 0.0007 | 0.0003 | 0.0003 | 0.0005 | 0.0006 | 0.0008 |
Bud Periphery | 0.0008 | 0.0005 | 0.0011 | 0.0015 | 0.0003 | 0.0016 | 0.0011 | 0.0001 | 0.0021 | 0.0008 | 0.0003 | 0.0007 | 0.0031 | 0.0013 | 0.0002 | 0.0002 | 0.0075 | 0.0003 |
Bud Site | 0.0009 | 0.003 | 0.0013 | 0.0009 | 0.0002 | 0.0003 | 0.0075 | 0.0006 | 0.0049 | 0.0023 | 0.0003 | 0.0004 | 0.0072 | 0.0096 | 0.0005 | 0.0003 | 0.0009 | 0.0001 |
Cell Periphery | 0.002 | 0.002 | 0.0013 | 0.0013 | 0.0003 | 0.0012 | 0.0012 | 0.0004 | 0.001 | 0.0003 | 0.0001 | 0.0014 | 0.0008 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.7731 | 0.9464 | 0.8775 | 0.7831 | 0.8579 | 0.8229 | 0.7264 | 0.9624 | 0.864 | 0.7538 | 0.8681 | 0.8706 | 0.8392 | 0.9617 | 0.9506 | 0.8826 | 0.9076 | 0.9334 |
Cytoplasmic Foci | 0.0032 | 0.0032 | 0.005 | 0.0054 | 0.0049 | 0.0066 | 0.0231 | 0.0061 | 0.009 | 0.0326 | 0.0089 | 0.0124 | 0.0211 | 0.0049 | 0.0092 | 0.0184 | 0.0285 | 0.0113 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.1169 | 0.0187 | 0.0412 | 0.0326 | 0.0557 | 0.1004 | 0.0593 | 0.0099 | 0.0254 | 0.0188 | 0.0043 | 0.047 | 0.0355 | 0.0025 | 0.0101 | 0.0046 | 0.0048 | 0.0189 |
Endosome | 0.0217 | 0.0063 | 0.0166 | 0.0333 | 0.0317 | 0.0218 | 0.0511 | 0.0093 | 0.0279 | 0.0781 | 0.0783 | 0.0314 | 0.0333 | 0.0056 | 0.0167 | 0.0551 | 0.0185 | 0.0203 |
Golgi | 0.0018 | 0.0009 | 0.0013 | 0.0014 | 0.0011 | 0.0026 | 0.01 | 0.001 | 0.0059 | 0.0201 | 0.0159 | 0.008 | 0.0107 | 0.0014 | 0.003 | 0.0128 | 0.0034 | 0.0039 |
Lipid Particles | 0.0004 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0085 | 0.0001 | 0.0018 | 0.0113 | 0.0017 | 0.0013 | 0.0062 | 0.0001 | 0.0001 | 0.0025 | 0.0012 | 0.0001 |
Mitochondria | 0.0096 | 0.0013 | 0.0056 | 0.0197 | 0.0041 | 0.0084 | 0.0096 | 0.0005 | 0.0173 | 0.0053 | 0.0067 | 0.0056 | 0.0051 | 0.0003 | 0.0009 | 0.0024 | 0.0004 | 0.0029 |
None | 0.001 | 0.0003 | 0.0004 | 0.0004 | 0.0004 | 0.0005 | 0.0088 | 0.0004 | 0.0015 | 0.0017 | 0.0007 | 0.0008 | 0.0023 | 0.0005 | 0.0004 | 0.0006 | 0.0017 | 0.0016 |
Nuclear Periphery | 0.0133 | 0.002 | 0.006 | 0.0083 | 0.005 | 0.0051 | 0.0117 | 0.0008 | 0.0041 | 0.004 | 0.0039 | 0.0041 | 0.0039 | 0.0004 | 0.0006 | 0.0009 | 0.001 | 0.0006 |
Nucleolus | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.001 | 0 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.0038 | 0.0017 | 0.0028 | 0.0035 | 0.003 | 0.0026 | 0.01 | 0.0009 | 0.0018 | 0.0026 | 0.0011 | 0.0019 | 0.0016 | 0.001 | 0.0007 | 0.0009 | 0.0011 | 0.0008 |
Peroxisomes | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0061 | 0.0001 | 0.0005 | 0.0103 | 0.0003 | 0.0008 | 0.0042 | 0 | 0.0002 | 0.0006 | 0.0004 | 0.0001 |
Punctate Nuclear | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0087 | 0.0001 | 0.0004 | 0.0028 | 0.0003 | 0.0007 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0013 | 0.0001 |
Vacuole | 0.0408 | 0.0096 | 0.0323 | 0.0858 | 0.0256 | 0.0143 | 0.0253 | 0.006 | 0.0109 | 0.0406 | 0.0035 | 0.0061 | 0.0065 | 0.0029 | 0.0037 | 0.0131 | 0.0036 | 0.0022 |
Vacuole Periphery | 0.0073 | 0.001 | 0.0043 | 0.0187 | 0.0069 | 0.004 | 0.0035 | 0.0005 | 0.0028 | 0.0063 | 0.0026 | 0.0012 | 0.0015 | 0.0004 | 0.0008 | 0.0031 | 0.001 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 93.8575 | 84.9988 | 102.6514 | 105.8623 | 101.909 | 72.975 | 68.8277 | 77.6173 | 82.0289 | 87.8054 |
Translational Efficiency | 0.8376 | 0.9594 | 0.9053 | 0.9324 | 0.7339 | 0.772 | 0.8375 | 0.6117 | 0.632 | 0.677 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2023 | 1599 | 1627 | 452 | 1536 | 1578 | 92 | 129 | 3559 | 3177 | 1719 | 581 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1036.05 | 1096.90 | 1663.82 | 1561.49 | 921.04 | 1205.83 | 1777.76 | 1651.04 | 986.41 | 1151.00 | 1669.92 | 1581.37 |
Standard Deviation | 162.67 | 229.98 | 250.35 | 266.30 | 187.36 | 195.24 | 230.62 | 273.47 | 182.86 | 220.27 | 250.65 | 270.48 |
Intensity Change Log 2 | — | 0.082338 | 0.683406 | 0.591830 | — | 0.388691 | 0.948725 | 0.842039 | — | 0.234635 | 0.814361 | 0.715000 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000163 | 0.000171 | 0.000246 | 0.000666 | 0.000224 | 0.000278 | 0.001008 | 0.001156 | 0.000189 | 0.000224 | 0.000287 | 0.000775 |
Bud Neck | 0.001007 | 0.000444 | 0.001066 | 0.001573 | 0.001808 | 0.000247 | 0.006669 | 0.003190 | 0.001353 | 0.000346 | 0.001366 | 0.001932 |
Bud Site | 0.024498 | 0.017712 | 0.008934 | 0.033974 | 0.026572 | 0.020567 | 0.026731 | 0.042732 | 0.025393 | 0.019130 | 0.009887 | 0.035919 |
Cell Periphery | 0.000156 | 0.000212 | 0.000519 | 0.001518 | 0.000223 | 0.000206 | 0.000493 | 0.002810 | 0.000185 | 0.000209 | 0.000517 | 0.001804 |
Cytoplasm | 0.804788* | 0.850408* | 0.905763* | 0.807684* | 0.724425* | 0.853809* | 0.524324* | 0.705674* | 0.770105* | 0.852097* | 0.885348* | 0.785034* |
Cytoplasmic Foci | 0.118175 | 0.072284 | 0.017245 | 0.038535 | 0.143284 | 0.087789 | 0.074237 | 0.083388 | 0.129012 | 0.079986 | 0.020295 | 0.048494 |
Eisosomes | 0.000008 | 0.000003 | 0.000002 | 0.000005 | 0.000017 | 0.000004 | 0.000012 | 0.000008 | 0.000012 | 0.000004 | 0.000002 | 0.000005 |
Endoplasmic Reticulum | 0.001341 | 0.001798 | 0.001419 | 0.000402 | 0.004673 | 0.000870 | 0.002156 | 0.000562 | 0.002779 | 0.001337 | 0.001458 | 0.000438 |
Endosome | 0.004693 | 0.006056 | 0.002839 | 0.006059 | 0.012307 | 0.003365 | 0.028352 | 0.011137 | 0.007979 | 0.004719 | 0.004205 | 0.007187 |
Golgi | 0.010280 | 0.008702 | 0.007323 | 0.012529 | 0.012925 | 0.014923 | 0.005992 | 0.012280 | 0.011421 | 0.011792 | 0.007251 | 0.012473 |
Lipid Particles | 0.000433 | 0.000627 | 0.003095 | 0.009656 | 0.001369 | 0.000388 | 0.013723 | 0.019694 | 0.000837 | 0.000508 | 0.003664 | 0.011884 |
Mitochondria | 0.000908 | 0.000288 | 0.000048 | 0.003331 | 0.001839 | 0.000969 | 0.000022 | 0.000011 | 0.001310 | 0.000626 | 0.000047 | 0.002594 |
Mitotic Spindle | 0.000461 | 0.000734 | 0.003554 | 0.020628 | 0.000628 | 0.001321 | 0.018450 | 0.017788 | 0.000533 | 0.001026 | 0.004351 | 0.019998 |
None | 0.000769 | 0.000553 | 0.000546 | 0.000233 | 0.002927 | 0.000303 | 0.000084 | 0.000066 | 0.001700 | 0.000429 | 0.000522 | 0.000196 |
Nuclear Periphery | 0.000009 | 0.000022 | 0.000112 | 0.000040 | 0.000438 | 0.000004 | 0.000494 | 0.000040 | 0.000194 | 0.000013 | 0.000132 | 0.000040 |
Nuclear Periphery Foci | 0.000160 | 0.000689 | 0.004344 | 0.001972 | 0.000547 | 0.000126 | 0.012881 | 0.003886 | 0.000327 | 0.000410 | 0.004800 | 0.002397 |
Nucleolus | 0.000052 | 0.000175 | 0.000045 | 0.000092 | 0.000401 | 0.000019 | 0.000085 | 0.000050 | 0.000203 | 0.000097 | 0.000047 | 0.000082 |
Nucleus | 0.003342 | 0.004133 | 0.008041 | 0.002583 | 0.007894 | 0.000978 | 0.011480 | 0.002257 | 0.005306 | 0.002566 | 0.008225 | 0.002511 |
Peroxisomes | 0.000443 | 0.000314 | 0.000089 | 0.001065 | 0.000561 | 0.000291 | 0.000545 | 0.001173 | 0.000494 | 0.000303 | 0.000114 | 0.001089 |
Vacuole | 0.019476 | 0.026332 | 0.030704 | 0.052413 | 0.041272 | 0.006828 | 0.266325* | 0.088829 | 0.028882 | 0.016645 | 0.043314 | 0.060499 |
Vacuole Periphery | 0.008839 | 0.008340 | 0.004067 | 0.005044 | 0.015666 | 0.006714 | 0.005940 | 0.003270 | 0.011785 | 0.007533 | 0.004167 | 0.004650 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.49 | -3.42 | -3.19 | -3.13 | -2.53 | -1.01 | -4.38 | -3.40 | -3.15 | -0.40 | -1.27 | -3.78 | -4.21 | -3.92 | -3.33 |
Bud Neck | 4.75 | -1.51 | -5.43 | -8.87 | -4.44 | 6.49 | -5.85 | -3.35 | -6.85 | 3.60 | 8.14 | -1.33 | -5.34 | -11.46 | -4.36 |
Bud Site | 4.47 | 10.55 | -2.89 | -4.75 | -7.05 | 3.45 | 0.16 | -3.06 | -4.25 | -2.53 | 5.40 | 13.08 | -3.69 | -5.71 | -8.55 |
Cell Periphery | -2.53 | -7.32 | -5.58 | -5.28 | -3.66 | 0.89 | -4.72 | -2.97 | -2.99 | -2.60 | -1.60 | -7.11 | -5.88 | -5.77 | -4.35 |
Cytoplasm | -6.48 | -15.14 | 3.54 | 6.53 | 10.02 | -15.28 | 6.71 | 1.96 | 6.93 | -4.03 | -15.08 | -17.65 | 2.73 | 9.07 | 10.84 |
Cytoplasmic Foci | 9.99 | 27.99 | 9.88 | 2.78 | -6.84 | 10.13 | 8.22 | 5.08 | -0.02 | -1.13 | 13.82 | 37.89 | 12.20 | 3.31 | -8.54 |
Eisosomes | 9.96 | 13.20 | 3.94 | -2.64 | -4.73 | 8.08 | 1.92 | 4.28 | -3.32 | 2.43 | 11.03 | 13.59 | 6.80 | -3.16 | -5.67 |
Endoplasmic Reticulum | -1.17 | -0.19 | 3.89 | 4.48 | 3.34 | 5.67 | 3.68 | 6.30 | 2.09 | 3.57 | 4.03 | 3.14 | 7.41 | 5.08 | 3.53 |
Endosome | -2.05 | 4.00 | -2.20 | -0.62 | -4.14 | 10.48 | -3.71 | 0.78 | -3.87 | 3.76 | 6.31 | 7.20 | 0.43 | -3.38 | -3.94 |
Golgi | 2.35 | 4.87 | -0.10 | -0.81 | -1.47 | -2.00 | 7.72 | 0.73 | 1.42 | -2.11 | -0.63 | 7.60 | 0.45 | 0.64 | -1.88 |
Lipid Particles | -2.92 | -10.85 | -6.99 | -6.87 | -5.19 | 7.08 | -5.00 | -4.33 | -4.55 | -1.08 | 4.70 | -10.27 | -8.03 | -8.23 | -6.03 |
Mitochondria | 6.08 | 9.11 | -0.84 | -1.14 | -1.26 | 1.44 | 3.64 | 3.67 | 2.80 | 3.04 | 2.44 | 5.69 | -0.47 | -0.89 | -1.26 |
Mitotic Spindle | -1.42 | -6.82 | -6.17 | -6.13 | -5.62 | -1.16 | -2.26 | -2.39 | -2.34 | -0.80 | -1.59 | -6.20 | -6.48 | -6.39 | -5.72 |
None | 1.45 | 1.64 | 3.55 | 3.25 | 3.82 | 3.20 | 3.43 | 3.46 | 5.74 | 1.04 | 3.49 | 3.24 | 4.02 | 3.60 | 4.70 |
Nuclear Periphery | -4.65 | -6.36 | -7.15 | -5.92 | 1.47 | 1.22 | -0.74 | 1.07 | -6.65 | 2.85 | 1.18 | 0.23 | 0.71 | -7.70 | 3.07 |
Nuclear Periphery Foci | -5.96 | -12.32 | -6.16 | -4.95 | 2.41 | 5.02 | -6.34 | -5.70 | -6.35 | 4.51 | -1.47 | -13.13 | -7.64 | -7.42 | 3.18 |
Nucleolus | -1.38 | 0.57 | -4.42 | 0.82 | -4.78 | 2.92 | 2.32 | 2.63 | -4.84 | 2.72 | 1.37 | 2.67 | 1.89 | 0.15 | -4.51 |
Nucleus | -1.62 | -9.33 | -0.66 | 0.76 | 7.26 | 6.11 | -2.65 | 3.94 | -4.96 | 4.89 | 4.83 | -5.37 | 2.74 | -2.18 | 8.67 |
Peroxisomes | 3.61 | 10.88 | -3.49 | -4.10 | -5.10 | 5.68 | 0.11 | -3.53 | -5.20 | -3.28 | 6.46 | 14.76 | -4.01 | -5.12 | -6.18 |
Vacuole | -3.28 | -6.04 | -7.97 | -6.64 | -5.36 | 13.88 | -9.01 | -4.44 | -7.22 | 6.11 | 7.79 | -5.94 | -8.19 | -10.50 | -4.55 |
Vacuole Periphery | 0.54 | 6.58 | 4.35 | 3.78 | -0.25 | 7.37 | 6.41 | 9.38 | 3.88 | 1.04 | 5.79 | 11.12 | 8.65 | 4.43 | 0.12 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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