Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function that contains 2 PY motifs; ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 6 | 21 | 8 | 11 | 16 |
Bud | 2 | 3 | 5 | 1 | 0 | 4 | 8 | 6 | 9 | 16 | 16 | 19 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 1 | 9 | 19 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 3 | 4 | 10 | 2 | 3 | 10 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 8 | 11 | 1 | 6 | 6 | 8 | 3 | 8 | 12 | 9 | 11 | 18 | 41 | 46 | 7 | 5 | 9 | 2 | 4 | 9 | 4 | 10 | 14 |
Cytoplasm | 205 | 328 | 414 | 215 | 323 | 373 | 503 | 360 | 268 | 441 | 309 | 348 | 401 | 553 | 482 | 581 | 332 | 299 | 195 | 295 | 319 | 166 | 318 | 335 |
Endoplasmic Reticulum | 3 | 5 | 2 | 1 | 0 | 2 | 2 | 0 | 3 | 0 | 7 | 5 | 0 | 2 | 0 | 43 | 28 | 18 | 4 | 6 | 12 | 0 | 6 | 8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 3 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 3 | 3 | 1 | 1 | 3 | 2 | 0 | 0 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 0 | 0 | 0 |
Mitochondria | 13 | 3 | 5 | 7 | 28 | 100 | 140 | 130 | 192 | 272 | 269 | 303 | 2 | 0 | 0 | 9 | 1 | 1 | 2 | 7 | 21 | 3 | 6 | 10 |
Nucleus | 0 | 1 | 2 | 1 | 0 | 3 | 5 | 4 | 0 | 5 | 2 | 2 | 0 | 0 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 1 | 1 | 4 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 1 | 0 | 2 | 4 |
Vac/Vac Membrane | 1 | 2 | 0 | 1 | 5 | 2 | 13 | 4 | 9 | 11 | 6 | 11 | 0 | 2 | 0 | 8 | 12 | 5 | 2 | 6 | 2 | 1 | 0 | 12 |
Unique Cell Count | 216 | 338 | 422 | 221 | 340 | 426 | 592 | 446 | 387 | 596 | 474 | 550 | 403 | 557 | 483 | 597 | 343 | 309 | 219 | 343 | 420 | 192 | 376 | 451 |
Labelled Cell Count | 226 | 350 | 439 | 227 | 364 | 497 | 688 | 514 | 490 | 761 | 623 | 709 | 423 | 602 | 529 | 658 | 385 | 340 | 219 | 343 | 420 | 192 | 376 | 451 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 5.3 | 4.7 | 4.7 | 4.6 | 4.0 | 3.7 | 4.1 | 3.8 | 3.3 | 3.7 | 3.4 | 5.8 | 5.7 | 5.8 | 5.7 | 6.7 | 6.5 | 5.0 | 5.3 | 6.3 |
Std Deviation (1e-4) | 0.5 | 0.8 | 1.1 | 1.4 | 1.4 | 1.3 | 0.9 | 1.3 | 1.0 | 1.2 | 1.3 | 1.1 | 0.8 | 1.1 | 1.2 | 1.4 | 1.6 | 1.4 | 0.8 | 1.1 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.0 | -0.03 | -0.2 | -0.34 | -0.2 | -0.3 | -0.51 | -0.34 | -0.47 | 0.31 | 0.3 | 0.31 | 0.3 | 0.52 | 0.48 | 0.1 | 0.19 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 3.3 | 4.3 | -1.7 | -1.1 | 0.3 |
Cytoplasm | -0.7 | -2.4 | -5.9 | -7.0 | -8.2 | -11.3 | -10.3 | -12.5 | -13.1 | 1.8 | 1.7 | 2.6 | -0.8 | -1.2 | -1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.1 | 5.4 | 4.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.2018 | 0.4758 | 0.2371 | 0.0134 | -0.1335 | 0.6337 | 2.4484 | 2.9962 | 2.9959 | 2.9248 | 2.9031 | 3.1697 | 4.7859 | 3.9376 | 4.2748 | 4.2167 | 3.7734 | 4.517 |
Actin | 0.0236 | 0.0004 | 0.0118 | 0.0041 | 0.0126 | 0.0039 | 0.0102 | 0.0016 | 0.0061 | 0.0016 | 0.014 | 0.002 | 0.0553 | 0.002 | 0.0054 | 0.0004 | 0.0049 | 0.0029 |
Bud | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.001 | 0.0004 | 0.001 | 0.0017 | 0.0002 | 0.0178 | 0.0007 | 0.001 | 0.0002 | 0.0031 | 0.0001 | 0.0286 | 0.0008 |
Bud Neck | 0.0031 | 0.0007 | 0.0006 | 0.0005 | 0.0028 | 0.0032 | 0.0088 | 0.0007 | 0.0009 | 0.0003 | 0.0006 | 0.0056 | 0.0193 | 0.0002 | 0.0053 | 0.0002 | 0.0003 | 0.0007 |
Bud Periphery | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0088 | 0.0007 | 0.0011 | 0.0047 | 0.0004 | 0.0146 | 0.0007 | 0.0021 | 0.0003 | 0.006 | 0.0003 | 0.0104 | 0.0006 |
Bud Site | 0.0012 | 0.0047 | 0.0055 | 0.0003 | 0.0007 | 0.0002 | 0.0035 | 0.0241 | 0.003 | 0.0002 | 0.0032 | 0.0003 | 0.0043 | 0.0026 | 0.0122 | 0.0001 | 0.0009 | 0.0002 |
Cell Periphery | 0.0024 | 0.0028 | 0.0015 | 0.0004 | 0.0008 | 0.0009 | 0.0017 | 0.0016 | 0.0026 | 0.0006 | 0.0005 | 0.0004 | 0.001 | 0.0013 | 0.0022 | 0.0008 | 0.0002 | 0.0003 |
Cytoplasm | 0.2704 | 0.3958 | 0.3343 | 0.2823 | 0.3209 | 0.3937 | 0.3216 | 0.3447 | 0.3626 | 0.2648 | 0.2692 | 0.3845 | 0.2793 | 0.5276 | 0.3732 | 0.1855 | 0.3417 | 0.3851 |
Cytoplasmic Foci | 0.0262 | 0.0034 | 0.0069 | 0.0255 | 0.0139 | 0.0038 | 0.0056 | 0.0045 | 0.0087 | 0.0054 | 0.0179 | 0.0078 | 0.0284 | 0.0053 | 0.0096 | 0.0032 | 0.0037 | 0.0046 |
Eisosomes | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0006 | 0.0002 | 0.0052 | 0.0002 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0011 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0055 | 0.0048 | 0.0056 | 0.0051 | 0.0025 | 0.0037 | 0.0047 | 0.0021 | 0.0052 | 0.0022 | 0.0041 | 0.0021 | 0.0092 | 0.0047 | 0.0047 | 0.0032 | 0.0021 | 0.0029 |
Endosome | 0.0069 | 0.0008 | 0.003 | 0.0029 | 0.0048 | 0.0007 | 0.0046 | 0.0006 | 0.0017 | 0.0014 | 0.0062 | 0.0031 | 0.0333 | 0.0011 | 0.0015 | 0.0007 | 0.0022 | 0.0023 |
Golgi | 0.0026 | 0.0002 | 0.0009 | 0.0008 | 0.0022 | 0.0002 | 0.002 | 0.0002 | 0.0005 | 0.0003 | 0.0028 | 0.0007 | 0.0077 | 0.0003 | 0.0005 | 0.0001 | 0.0009 | 0.0003 |
Lipid Particles | 0.0045 | 0.0008 | 0.0024 | 0.0018 | 0.0055 | 0.0007 | 0.0042 | 0.0004 | 0.0008 | 0.0007 | 0.009 | 0.0006 | 0.0118 | 0.0005 | 0.0009 | 0.0002 | 0.004 | 0.0006 |
Mitochondria | 0.0006 | 0.0001 | 0.0003 | 0.0005 | 0.0005 | 0.0002 | 0.0018 | 0.0002 | 0.0005 | 0.0003 | 0.0009 | 0.0009 | 0.0035 | 0.0002 | 0.0004 | 0.0001 | 0.0013 | 0.0003 |
None | 0.6332 | 0.5821 | 0.6195 | 0.6657 | 0.6215 | 0.5769 | 0.6177 | 0.612 | 0.595 | 0.7188 | 0.5892 | 0.5866 | 0.4889 | 0.45 | 0.5618 | 0.8022 | 0.5416 | 0.5875 |
Nuclear Periphery | 0.0022 | 0.0004 | 0.0009 | 0.0008 | 0.0012 | 0.0003 | 0.002 | 0.0004 | 0.0013 | 0.0004 | 0.0033 | 0.0004 | 0.0124 | 0.0005 | 0.0019 | 0.0005 | 0.0228 | 0.0024 |
Nucleolus | 0.0007 | 0.0002 | 0.0005 | 0.0002 | 0.0007 | 0.0001 | 0.0007 | 0.0004 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0033 | 0.0002 | 0.0006 | 0.0001 | 0.0022 | 0.0003 |
Nucleus | 0.0017 | 0.0009 | 0.0017 | 0.0008 | 0.0007 | 0.0005 | 0.0009 | 0.0013 | 0.0013 | 0.0004 | 0.0018 | 0.0009 | 0.0071 | 0.0011 | 0.0013 | 0.0007 | 0.0047 | 0.0028 |
Peroxisomes | 0.0036 | 0.0001 | 0.0002 | 0.0034 | 0.0027 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.028 | 0.0003 | 0.0074 | 0.0003 | 0.0006 | 0.0001 | 0.001 | 0.0005 |
Punctate Nuclear | 0.0095 | 0.0004 | 0.002 | 0.0033 | 0.0042 | 0.0004 | 0.0014 | 0.0016 | 0.0013 | 0.0005 | 0.0152 | 0.0012 | 0.0165 | 0.0006 | 0.0072 | 0.0005 | 0.025 | 0.004 |
Vacuole | 0.001 | 0.001 | 0.0013 | 0.0007 | 0.0005 | 0.0006 | 0.0013 | 0.0008 | 0.0011 | 0.0006 | 0.0008 | 0.0009 | 0.0053 | 0.0008 | 0.0009 | 0.0007 | 0.0006 | 0.0005 |
Vacuole Periphery | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0016 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.8977 | 3.1676 | 3.7607 | 8.245 | 4.5941 | 6.3745 | 8.4872 | 7.998 | 9.9294 | 8.5903 |
Translational Efficiency | 1.2458 | 1.211 | 0.9685 | 0.5873 | 0.611 | 0.7923 | 0.5914 | 0.7836 | 0.6378 | 0.6403 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1104 | 1629 | 336 | 1702 | 1339 | 1483 | 342 | 2360 | 2443 | 3112 | 678 | 4062 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 633.64 | 671.85 | 948.48 | 832.97 | 706.07 | 715.18 | 970.32 | 877.34 | 673.34 | 692.50 | 959.50 | 858.75 |
Standard Deviation | 66.98 | 88.31 | 112.46 | 111.03 | 75.62 | 88.99 | 104.62 | 116.06 | 80.38 | 91.24 | 109.12 | 116.06 |
Intensity Change Log 2 | — | 0.084476 | 0.581954 | 0.394601 | — | 0.018495 | 0.458649 | 0.313325 | — | 0.050078 | 0.518284 | 0.352337 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000075 | 0.000435 | 0.002357 | 0.001059 | 0.000216 | 0.000519 | 0.000758 | 0.001258 | 0.000152 | 0.000475 | 0.001551 | 0.001175 |
Bud Neck | 0.005732 | 0.044209 | 0.005896 | 0.016031 | 0.008629 | 0.044802 | 0.003980 | 0.018020 | 0.007320 | 0.044492 | 0.004929 | 0.017187 |
Bud Site | 0.002068 | 0.014367 | 0.013738 | 0.041179 | 0.002540 | 0.024406 | 0.018470 | 0.044983 | 0.002326 | 0.019151 | 0.016125 | 0.043389 |
Cell Periphery | 0.000198 | 0.000152 | 0.000700 | 0.000518 | 0.000381 | 0.000185 | 0.000606 | 0.000301 | 0.000298 | 0.000168 | 0.000653 | 0.000392 |
Cytoplasm | 0.546572* | 0.402916* | 0.201774* | 0.482987* | 0.513917* | 0.374692* | 0.270782* | 0.538838* | 0.528674* | 0.389466* | 0.236583* | 0.515436* |
Cytoplasmic Foci | 0.125837 | 0.244636* | 0.021411 | 0.013740 | 0.138349 | 0.261981* | 0.026204 | 0.011564 | 0.132695 | 0.252902* | 0.023828 | 0.012476 |
Eisosomes | 0.000126 | 0.000190 | 0.000183 | 0.000075 | 0.000341 | 0.000206 | 0.000208 | 0.000052 | 0.000244 | 0.000197 | 0.000196 | 0.000062 |
Endoplasmic Reticulum | 0.000404 | 0.001022 | 0.019708 | 0.002795 | 0.000794 | 0.001099 | 0.010086 | 0.001490 | 0.000618 | 0.001059 | 0.014854 | 0.002037 |
Endosome | 0.004015 | 0.019158 | 0.005399 | 0.001704 | 0.004732 | 0.031529 | 0.007005 | 0.003734 | 0.004408 | 0.025053 | 0.006209 | 0.002883 |
Golgi | 0.000926 | 0.005526 | 0.000144 | 0.000284 | 0.001082 | 0.008137 | 0.000152 | 0.001450 | 0.001012 | 0.006770 | 0.000148 | 0.000962 |
Lipid Particles | 0.004809 | 0.008576 | 0.004750 | 0.001159 | 0.006903 | 0.009011 | 0.004903 | 0.000893 | 0.005957 | 0.008784 | 0.004827 | 0.001004 |
Mitochondria | 0.002306 | 0.013651 | 0.000574 | 0.001172 | 0.000896 | 0.018580 | 0.000393 | 0.004559 | 0.001533 | 0.016000 | 0.000483 | 0.003140 |
Mitotic Spindle | 0.001701 | 0.003242 | 0.000554 | 0.021731 | 0.001743 | 0.011689 | 0.003940 | 0.041635 | 0.001724 | 0.007267 | 0.002262 | 0.033295 |
None | 0.005755 | 0.003654 | 0.001728 | 0.007584 | 0.003289 | 0.002702 | 0.002643 | 0.005123 | 0.004404 | 0.003200 | 0.002189 | 0.006154 |
Nuclear Periphery | 0.000384 | 0.000384 | 0.002090 | 0.001173 | 0.000347 | 0.000521 | 0.001586 | 0.001220 | 0.000364 | 0.000449 | 0.001836 | 0.001201 |
Nuclear Periphery Foci | 0.000388 | 0.000430 | 0.004671 | 0.000948 | 0.000541 | 0.000306 | 0.005948 | 0.000816 | 0.000472 | 0.000371 | 0.005315 | 0.000871 |
Nucleolus | 0.001548 | 0.002191 | 0.000583 | 0.000290 | 0.000813 | 0.001549 | 0.000522 | 0.000371 | 0.001145 | 0.001885 | 0.000552 | 0.000337 |
Nucleus | 0.266910* | 0.144048* | 0.532890* | 0.302206* | 0.274713* | 0.101357 | 0.349954* | 0.231494* | 0.271187* | 0.123704 | 0.440612* | 0.261123* |
Peroxisomes | 0.001817 | 0.014034 | 0.000756 | 0.000284 | 0.002669 | 0.022816 | 0.000768 | 0.001705 | 0.002284 | 0.018219 | 0.000762 | 0.001110 |
Vacuole | 0.028141 | 0.075119 | 0.179894 | 0.101754 | 0.036858 | 0.080866 | 0.290875* | 0.088979 | 0.032919 | 0.077858 | 0.235876* | 0.094332 |
Vacuole Periphery | 0.000288 | 0.002060 | 0.000201 | 0.001328 | 0.000246 | 0.003047 | 0.000216 | 0.001515 | 0.000265 | 0.002531 | 0.000208 | 0.001436 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.23 | -3.15 | -3.83 | -1.72 | 1.94 | -2.31 | -4.19 | -3.79 | -2.23 | -1.22 | -4.00 | -3.84 | -5.41 | -2.89 | 1.55 |
Bud Neck | -13.13 | -0.75 | -5.32 | 10.60 | -6.22 | -12.91 | 5.16 | -5.10 | 10.48 | -10.93 | -18.38 | 2.56 | -7.39 | 14.82 | -11.96 |
Bud Site | -9.87 | -3.98 | -9.73 | -4.03 | -3.76 | -9.67 | -2.86 | -12.44 | -0.72 | -4.23 | -13.38 | -4.72 | -15.77 | -3.45 | -5.75 |
Cell Periphery | 1.80 | -7.70 | -4.26 | -6.55 | 4.55 | 8.56 | -1.60 | 5.69 | -3.12 | 4.48 | 7.64 | -6.62 | 0.14 | -7.41 | 6.69 |
Cytoplasm | 14.32 | 29.39 | 16.63 | 2.36 | -17.18 | 15.04 | 23.18 | 9.23 | -6.89 | -17.32 | 20.55 | 36.91 | 17.90 | -3.71 | -24.75 |
Cytoplasmic Foci | -16.93 | 22.79 | 26.27 | 42.34 | 2.44 | -17.51 | 27.03 | 31.51 | 43.58 | 4.89 | -24.18 | 35.29 | 41.00 | 60.75 | 5.10 |
Eisosomes | -3.59 | -5.26 | 4.05 | 6.05 | 8.70 | 6.64 | 6.91 | 16.89 | 10.11 | 9.47 | 3.47 | 3.45 | 16.80 | 11.23 | 13.10 |
Endoplasmic Reticulum | -5.26 | -13.32 | -18.58 | -13.98 | 10.87 | -1.29 | -12.70 | -12.55 | -6.50 | 10.14 | -3.47 | -16.83 | -22.21 | -14.26 | 13.82 |
Endosome | -13.85 | -3.50 | 5.85 | 17.55 | 10.92 | -15.26 | -2.13 | 4.71 | 17.02 | 7.13 | -20.24 | -3.86 | 7.02 | 23.50 | 11.83 |
Golgi | -8.25 | 2.63 | 2.59 | 11.28 | 0.05 | -8.05 | 3.82 | 1.53 | 8.77 | -2.54 | -11.32 | 4.57 | 2.70 | 13.14 | -2.45 |
Lipid Particles | -4.13 | -0.39 | 4.62 | 12.16 | 9.41 | -2.31 | 3.54 | 9.26 | 11.47 | 10.13 | -4.42 | 2.21 | 9.88 | 16.75 | 13.80 |
Mitochondria | -6.91 | 1.93 | 1.53 | 9.23 | -2.73 | -9.25 | 3.44 | -3.22 | 8.14 | -4.49 | -11.75 | 2.60 | -0.67 | 11.82 | -4.85 |
Mitotic Spindle | -1.46 | 0.97 | -5.87 | -4.96 | -6.78 | -4.75 | -0.17 | -9.51 | -4.52 | -8.52 | -4.72 | 0.33 | -11.17 | -7.05 | -11.08 |
None | 2.02 | 5.71 | 0.66 | -1.41 | -5.11 | 0.57 | 2.19 | -2.13 | -2.64 | -5.50 | 1.70 | 5.55 | -0.85 | -2.71 | -7.74 |
Nuclear Periphery | -0.06 | -19.23 | -19.37 | -19.46 | 2.62 | -1.93 | -18.31 | -21.36 | -14.25 | -2.57 | -1.83 | -26.25 | -28.65 | -23.30 | 0.11 |
Nuclear Periphery Foci | -0.75 | -11.91 | -8.02 | -7.19 | 9.15 | 2.82 | -9.53 | -4.01 | -6.93 | 8.47 | 1.65 | -14.83 | -8.04 | -9.63 | 12.42 |
Nucleolus | -1.29 | 1.91 | 2.36 | 7.28 | 3.88 | -2.99 | 1.77 | 1.48 | 7.50 | -0.99 | -2.68 | 2.49 | 2.75 | 9.74 | 1.31 |
Nucleus | 16.01 | -14.37 | -8.70 | -26.88 | 9.92 | 25.78 | -5.19 | -1.50 | -30.27 | 4.54 | 28.90 | -13.66 | -6.88 | -39.03 | 10.48 |
Peroxisomes | -12.55 | 2.86 | 4.12 | 15.54 | 4.13 | -11.46 | 4.06 | 3.22 | 13.01 | -1.56 | -16.16 | 4.94 | 4.77 | 18.91 | 0.03 |
Vacuole | -14.99 | -15.41 | -27.73 | -17.93 | 3.05 | -12.97 | -21.14 | -28.71 | -17.23 | 11.24 | -19.45 | -25.36 | -39.93 | -25.00 | 10.25 |
Vacuole Periphery | -9.87 | 1.84 | -0.72 | 3.60 | -0.92 | -10.05 | 2.04 | -1.83 | 6.81 | -2.18 | -13.91 | 2.69 | -1.71 | 7.42 | -2.07 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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