Standard name | |
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Human Ortholog | |
Description | ATP-binding protein that is a subunit of the HOPS and CORVET complexes; essential for protein sorting, vesicle docking, and fusion at the vacuole; binds to SNARE domains |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 3 | 4 | 14 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 2 | 2 | 3 | 3 | 8 | 29 | 21 | 18 | 31 | 26 | 33 | 0 | 1 | 0 | 2 | 3 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 1 | 3 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 3 | 5 | 11 | 9 | 11 | 14 | 18 | 7 | 18 | 7 | 10 | 1 | 13 | 16 | 25 | 21 | 19 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 2 | 1 | 1 | 2 | 10 | 9 | 11 | 1 | 0 | 0 | 0 | 0 | 1 |
Endosome | 128 | 37 | 198 | 138 | 221 | 89 | 207 | 94 | 56 | 33 | 40 | 71 | 23 | 40 | 47 | 143 | 142 | 119 | 170 | 183 | 280 | 220 | 323 | 324 |
Golgi | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 5 | 1 | 4 | 4 | 3 | 1 | 3 | 6 |
Mitochondria | 51 | 123 | 71 | 68 | 98 | 166 | 252 | 169 | 276 | 295 | 235 | 294 | 0 | 1 | 0 | 42 | 23 | 17 | 5 | 7 | 1 | 6 | 7 | 9 |
Nucleus | 1 | 5 | 2 | 0 | 0 | 0 | 3 | 2 | 3 | 3 | 1 | 0 | 2 | 4 | 5 | 3 | 0 | 0 | 1 | 3 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 6 | 8 | 5 | 4 | 4 | 2 | 2 | 1 | 2 | 5 | 2 | 3 | 3 | 4 | 0 | 5 | 8 | 4 | 2 | 2 | 1 | 2 | 7 | 8 |
Nucleolus | 65 | 44 | 103 | 50 | 64 | 41 | 59 | 28 | 49 | 43 | 34 | 39 | 14 | 21 | 24 | 17 | 17 | 15 | 1 | 2 | 1 | 5 | 5 | 2 |
Peroxisomes | 1 | 0 | 16 | 15 | 16 | 21 | 82 | 52 | 16 | 54 | 28 | 41 | 0 | 0 | 0 | 2 | 3 | 0 | 2 | 1 | 5 | 1 | 1 | 4 |
SpindlePole | 36 | 11 | 70 | 39 | 52 | 52 | 121 | 93 | 43 | 92 | 54 | 90 | 48 | 85 | 81 | 61 | 37 | 24 | 8 | 8 | 12 | 13 | 10 | 8 |
Vac/Vac Membrane | 50 | 78 | 37 | 19 | 26 | 19 | 24 | 21 | 16 | 6 | 8 | 20 | 35 | 56 | 62 | 43 | 54 | 45 | 9 | 30 | 9 | 10 | 19 | 31 |
Unique Cell Count | 199 | 216 | 295 | 216 | 322 | 300 | 528 | 353 | 365 | 441 | 326 | 444 | 102 | 191 | 188 | 229 | 210 | 168 | 208 | 247 | 321 | 267 | 386 | 405 |
Labelled Cell Count | 345 | 313 | 510 | 348 | 494 | 410 | 800 | 506 | 490 | 586 | 442 | 612 | 132 | 242 | 242 | 353 | 323 | 255 | 208 | 247 | 321 | 267 | 386 | 405 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.9 | 6.6 | 6.0 | 5.9 | 5.4 | 4.8 | 4.8 | 5.0 | 4.7 | 4.3 | 4.6 | 4.7 | 9.5 | 9.7 | 10.2 | 5.0 | 6.0 | 7.3 | 8.6 | 9.0 | 9.4 |
Std Deviation (1e-4) | 0.9 | 1.0 | 1.0 | 1.3 | 1.1 | 1.1 | 1.1 | 1.3 | 0.8 | 1.0 | 0.8 | 0.9 | 0.8 | 1.1 | 1.1 | 0.8 | 1.4 | 1.2 | 1.2 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.16 | -0.32 | -0.32 | -0.27 | -0.37 | -0.51 | -0.39 | -0.37 | 0.66 | 0.68 | 0.75 | -0.28 | -0.0 | 0.27 | 0.52 | 0.58 | 0.63 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.7 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.2 | 0.9 | 1.5 | 0.9 | 2.3 | 0.2 | 1.8 | 0.4 | 0.5 | 0 | 2.9 | 3.6 | 4.5 | 4.2 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.8 | 0.4 | 0 | 0 | -10.3 | 0 | 0 | 0 | 0 | -7.8 | -9.9 | -9.0 | -1.1 | 0.1 | 0.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.9 | 1.8 | 0 | 0 | 6.2 | 0 | 0 | 0 | 0 | -5.5 | -7.1 | -7.3 | -1.6 | -3.7 | -3.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 |
Nucleolus | -2.9 | -4.2 | 0 | 0 | -8.5 | 0 | 0 | 0 | 0 | -4.0 | -5.9 | -5.4 | -7.4 | -7.0 | -6.2 |
Peroxisomes | 0.7 | -0.3 | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | -3.3 | -3.2 | -2.8 | -2.3 | -3.1 |
SpindlePole | -1.5 | -2.4 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | 4.4 | 4.8 | 4.5 | 0.8 | -1.7 | -2.4 |
Vacuole | -1.3 | -1.8 | -2.6 | -4.2 | -2.9 | -3.8 | -6.3 | -4.8 | -4.0 | 4.9 | 4.6 | 5.4 | 2.0 | 3.8 | 3.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8644 | 3.4072 | 3.0526 | 3.0097 | 2.7398 | 2.9 | 3.5191 | 3.7211 | 3.275 | 3.0928 | 2.886 | 3.2342 | 3.1842 | 4.1122 | 3.7498 | 3.8933 | 2.7724 | 3.6426 |
Actin | 0.0067 | 0.0001 | 0.0102 | 0 | 0.0002 | 0 | 0.002 | 0.0001 | 0.0027 | 0 | 0 | 0.0001 | 0.0071 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 |
Bud | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Bud Neck | 0.0005 | 0.0007 | 0.0006 | 0.0001 | 0.0003 | 0.0006 | 0.0023 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0007 | 0.0003 | 0.0001 | 0.0005 | 0.0003 |
Bud Periphery | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0004 | 0.0009 | 0.0008 | 0.0001 | 0.0001 | 0.0003 | 0.0045 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0 | 0.0004 | 0.0002 |
Cell Periphery | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 |
Cytoplasm | 0.0033 | 0.0023 | 0.0012 | 0.0009 | 0.0007 | 0.0007 | 0.0024 | 0.0045 | 0.0009 | 0.0005 | 0.0004 | 0.0003 | 0.0009 | 0.0014 | 0.0006 | 0.0004 | 0.0006 | 0.0004 |
Cytoplasmic Foci | 0.1015 | 0.1333 | 0.1166 | 0.1593 | 0.0837 | 0.1073 | 0.1435 | 0.1599 | 0.1683 | 0.1545 | 0.1747 | 0.1167 | 0.1251 | 0.18 | 0.1487 | 0.1956 | 0.1303 | 0.1071 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0067 | 0.0005 | 0.0004 | 0.0001 | 0.0007 | 0.0007 | 0.0004 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0005 | 0.0002 | 0 | 0.0003 | 0.0002 |
Endosome | 0.524 | 0.4996 | 0.514 | 0.5573 | 0.6406 | 0.4981 | 0.4309 | 0.3548 | 0.3719 | 0.5763 | 0.5215 | 0.3248 | 0.4465 | 0.3675 | 0.4072 | 0.58 | 0.4998 | 0.3542 |
Golgi | 0.0562 | 0.0545 | 0.0799 | 0.1134 | 0.0699 | 0.0926 | 0.0582 | 0.0544 | 0.0855 | 0.0777 | 0.0568 | 0.087 | 0.0621 | 0.0486 | 0.0876 | 0.085 | 0.0779 | 0.0774 |
Lipid Particles | 0.1707 | 0.1883 | 0.1613 | 0.0961 | 0.0895 | 0.2209 | 0.2525 | 0.3335 | 0.2862 | 0.1469 | 0.1589 | 0.3919 | 0.2805 | 0.2994 | 0.2711 | 0.113 | 0.2018 | 0.3849 |
Mitochondria | 0.0037 | 0.0026 | 0.0097 | 0.0013 | 0.0083 | 0.0024 | 0.0102 | 0.0017 | 0.0037 | 0.0007 | 0.0007 | 0.0021 | 0.0024 | 0.0061 | 0.0072 | 0.0005 | 0.0018 | 0.0028 |
None | 0.002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0023 | 0.0015 | 0.0009 | 0 | 0 | 0.0001 | 0.0013 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0141 | 0.0024 | 0.0005 | 0.0001 | 0.033 | 0.0022 | 0.0007 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0017 | 0.0012 | 0.0003 | 0 | 0.0021 | 0.0005 |
Nucleolus | 0.001 | 0.0053 | 0.0086 | 0.0001 | 0.0012 | 0.0025 | 0.0026 | 0.0017 | 0.0005 | 0.0001 | 0.0003 | 0.0008 | 0.001 | 0.0019 | 0.0009 | 0.0002 | 0.0081 | 0.0016 |
Nucleus | 0.0027 | 0.0008 | 0.0016 | 0 | 0.0027 | 0.0006 | 0.0012 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0032 | 0.0003 |
Peroxisomes | 0.0269 | 0.0222 | 0.0424 | 0.0426 | 0.0187 | 0.0258 | 0.0255 | 0.0429 | 0.0594 | 0.0319 | 0.0517 | 0.0577 | 0.0362 | 0.0493 | 0.0551 | 0.0196 | 0.0503 | 0.0444 |
Punctate Nuclear | 0.0027 | 0.0024 | 0.0075 | 0.0005 | 0.0014 | 0.002 | 0.0067 | 0.0192 | 0.0043 | 0.0004 | 0.0004 | 0.012 | 0.0022 | 0.0117 | 0.0022 | 0.0004 | 0.0038 | 0.0016 |
Vacuole | 0.0562 | 0.0639 | 0.036 | 0.0188 | 0.0366 | 0.0312 | 0.0409 | 0.0193 | 0.0081 | 0.0067 | 0.0265 | 0.0035 | 0.0235 | 0.0237 | 0.0132 | 0.0032 | 0.0129 | 0.0172 |
Vacuole Periphery | 0.0201 | 0.0196 | 0.0081 | 0.009 | 0.0119 | 0.0119 | 0.012 | 0.0039 | 0.0058 | 0.0039 | 0.0075 | 0.0024 | 0.0076 | 0.0069 | 0.0044 | 0.0017 | 0.0057 | 0.0066 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.8959 | 10.9281 | 9.5194 | 13.7284 | 15.8497 | 12.7491 | 13.2112 | 12.1591 | 12.5246 | 15.5416 |
Translational Efficiency | 0.5946 | 0.6434 | 0.853 | 0.5371 | 0.457 | 0.4687 | 0.695 | 0.5343 | 0.6449 | 0.5464 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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