Standard name
Human Ortholog
Description Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0.07 0.08 0.68 0.84 0.78 – 0.58 0.64 0.39 0.59 – 0.31 0.68 0.08 0.06 0.09 0 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – 0.06 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0.11 0.12 0.14 0 0 0 0.05 0 0.06
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.14 – 0.44 0.31 0.71 0.37 – 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.88 0.84 0.81 0.86 0.17 0 0.1 – 0.09 0.13 0.09 0.09 – 0.5 0.12 0.58 0.63 0.51 0.86 0.8 0.75 0.69 0.77 0.59
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – 0.06 0 0.14 0.14 0.15 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – 0.06 0 0.09 0.12 0.1 0 0 0 0 0 0.07
Vac/Vac Membrane 0.08 0.15 0.11 0.05 0.16 0.08 0.05 – 0 0 0 0 – 0.06 0.19 0.19 0.09 0.2 0.07 0.08 0.13 0.08 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 0 1 0 – 0 0 0 0 0 0 1 0 0 0 0
Bud 1 1 0 1 0 1 0 – 2 2 3 1 – 0 0 0 1 0 3 5 2 0 1 3
Bud Neck 0 0 1 0 1 1 5 – 2 7 0 4 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 1 3 1 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 1 – 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 16 10 12 109 186 218 – 102 135 94 134 – 5 50 13 13 16 2 6 2 2 1 2
Endoplasmic Reticulum 1 0 0 0 1 0 0 – 0 0 0 0 – 1 0 5 5 3 1 0 2 0 0 0
Endosome 1 1 0 2 1 0 2 – 0 1 1 0 – 0 3 18 25 25 2 2 2 2 2 3
Golgi 0 1 1 0 0 0 0 – 0 0 0 0 – 0 0 2 4 4 2 2 1 0 0 0
Mitochondria 12 6 0 6 3 25 38 – 78 65 170 84 – 0 1 9 4 6 3 3 0 0 0 0
Nucleus 241 244 114 133 28 5 27 – 16 28 22 20 – 8 9 98 129 93 262 248 115 29 48 30
Nuclear Periphery 0 0 0 1 0 0 0 – 0 0 0 1 – 0 1 1 1 1 0 0 0 0 0 0
Nucleolus 0 1 1 2 1 0 0 – 0 0 1 1 – 1 0 24 29 28 0 3 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 2 2 1 0 0 0 0 0 0
SpindlePole 1 0 2 2 3 2 4 – 3 2 4 2 – 1 1 15 24 19 3 8 3 1 2 3
Vac/Vac Membrane 22 44 15 7 26 17 13 – 3 7 6 7 – 1 14 32 19 36 22 26 20 3 4 3
Unique Cell Count 273 289 140 155 160 222 278 177 212 238 228 16 73 169 206 181 306 311 155 43 64 52
Labelled Cell Count 285 314 144 166 174 238 308 207 250 303 255 17 79 219 256 232 306 311 155 43 64 52


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 6.4 7.0 5.9 5.5 4.3 4.2 – 3.6 3.9 3.4 3.7 – 6.1 5.6 9.9 9.9 9.2 – 7.2 –
Std Deviation (1e-4) 0.8 1.1 1.5 1.1 1.4 1.5 1.2 – 0.8 1.0 0.9 1.1 – 1.8 1.4 2.7 2.4 2.4 – 1.6 –
Intensity Change (Log2) – – – -0.24 -0.34 -0.69 -0.72 – -0.97 -0.83 -1.03 -0.9 – -0.2 -0.32 0.51 0.5 0.41 – 0.05 –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0.2 10.8 14.3 13.8 – 9.3 10.5 6.8 9.9 – 0 9.4 0.2 -0.3 0.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – 0 0 4.0 4.3 4.6
Golgi 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Nucleus 1.0 -11.1 -15.6 -14.6 – -13.0 -12.8 -14.1 -14.1 – -2.9 -9.7 -4.4 -3.8 -5.6
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – 0 0 4.3 4.3 4.6
Peroxisomes 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – 0 0 2.9 3.5 3.3
Vacuole -2.0 1.4 -1.0 -2.3 – -3.4 -2.8 -3.4 -3.0 – 0 1.7 2.0 -0.5 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7726 1.5538 0.9519 1.0915 0.6866 1.0784 1.0945 1.1467 1.0974 1.0102 1.0553 1.1514 0.5108 0.9399 0.6913 1.0124 1.0045 0.6855
Actin 0.0096 0.0001 0.0045 0.0001 0.0239 0.0032 0.0118 0.0001 0.0019 0.0033 0.0055 0.0004 0.0089 0.0001 0.0113 0.0027 0.0345 0.0008
Bud 0.0003 0.0026 0 0 0.0015 0.0008 0.0005 0 0.0005 0.0002 0.0025 0 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001
Bud Neck 0.0011 0.0001 0.0001 0.0005 0.0078 0.0031 0.0088 0.0002 0.0034 0.003 0.0015 0.004 0.0005 0.0003 0.0008 0.0006 0.0011 0.0011
Bud Periphery 0.0004 0.0003 0 0.0001 0.0028 0.0003 0.0016 0 0.001 0.0004 0.0087 0 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001
Bud Site 0.0013 0.0047 0.0001 0.0002 0.0153 0.0002 0.0035 0.0001 0.0024 0.0009 0.0044 0 0.001 0.0004 0.0004 0.0002 0.0004 0.0001
Cell Periphery 0.0001 0.0001 0 0 0.0007 0 0.0005 0 0.0001 0.0002 0.0007 0 0.0001 0 0 0 0.0001 0
Cytoplasm 0.0053 0.0058 0.0025 0.0101 0.0207 0.0037 0.0129 0.0094 0.0063 0.006 0.0195 0.0043 0.0025 0.019 0.0141 0.056 0.0569 0.0079
Cytoplasmic Foci 0.0086 0.0003 0.0005 0.0057 0.0099 0.0027 0.0093 0.0003 0.0017 0.0047 0.0128 0.0009 0.0038 0.0001 0.0003 0.0004 0.0028 0.0002
Eisosomes 0.0002 0 0 0 0.0008 0 0.0003 0 0 0.0008 0.0001 0 0.0003 0 0.0004 0 0.0003 0
Endoplasmic Reticulum 0.0016 0.0002 0.0001 0.001 0.0038 0.0008 0.0022 0.0005 0.002 0.0007 0.0035 0.0001 0.0007 0.0003 0.0004 0.0022 0.0038 0.0007
Endosome 0.0132 0.0005 0.001 0.0094 0.0264 0.0033 0.0092 0.0008 0.0031 0.0042 0.0269 0.0006 0.0108 0.0002 0.0008 0.0083 0.0154 0.0038
Golgi 0.0025 0 0.0017 0.0002 0.0116 0.0015 0.0036 0 0.0005 0.0016 0.0051 0.0001 0.0034 0 0.0005 0.0005 0.0041 0.0003
Lipid Particles 0.0092 0 0.0003 0.003 0.0182 0.0039 0.0145 0.0001 0.0027 0.0146 0.0107 0.0005 0.0027 0 0.0002 0.0004 0.0061 0.0003
Mitochondria 0.0067 0.0003 0.0003 0.0006 0.0506 0.0045 0.0052 0.0003 0.0007 0.0086 0.0174 0.0001 0.0013 0.0002 0.0005 0.0015 0.0018 0.0011
None 0.0212 0.0028 0.0194 0.0061 0.0805 0.0114 0.0196 0.0226 0.0209 0.0213 0.0374 0.0036 0.0156 0.0093 0.027 0.0186 0.0059 0.0014
Nuclear Periphery 0.0137 0.0148 0.0064 0.0095 0.0306 0.0058 0.02 0.0165 0.0098 0.0184 0.0339 0.0038 0.0115 0.005 0.014 0.0359 0.0342 0.0133
Nucleolus 0.0131 0.005 0.0065 0.0076 0.0123 0.0187 0.0131 0.007 0.0095 0.016 0.0108 0.0116 0.005 0.0033 0.0043 0.0035 0.0059 0.0064
Nucleus 0.8318 0.9515 0.9332 0.9017 0.5774 0.9101 0.8168 0.9276 0.916 0.8518 0.7528 0.9425 0.9061 0.9503 0.9074 0.8608 0.808 0.9555
Peroxisomes 0.0037 0 0.0002 0.0002 0.0048 0.0007 0.0155 0 0.0031 0.023 0.0116 0.0016 0.0035 0 0.0002 0.0001 0.0011 0.0001
Punctate Nuclear 0.0541 0.0098 0.0226 0.0408 0.092 0.0246 0.0256 0.0139 0.0136 0.0178 0.0234 0.0257 0.0201 0.0107 0.0164 0.0059 0.0158 0.0056
Vacuole 0.0013 0.0007 0.0002 0.0026 0.0048 0.0005 0.0038 0.0004 0.0005 0.0015 0.0077 0.0001 0.0011 0.0004 0.0004 0.0011 0.0007 0.0006
Vacuole Periphery 0.0013 0.0002 0.0001 0.0005 0.0035 0.0004 0.0018 0.0002 0.0002 0.0011 0.0031 0 0.0005 0.0001 0.0002 0.0011 0.0009 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.9629 31.9454 43.3982 34.8466 41.6995 25.8253 34.1634 30.6881 26.6107 33.0872
Translational Efficiency 1.5703 1.6585 1.1307 1.074 0.9611 1.26 1.0836 0.9092 1.0028 0.978

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
127 1228 950 41 1656 2021 1330 55 1783 3249 2280 96

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 718.86 791.14 985.70 330.88 725.44 808.05 1073.05 1015.21 724.97 801.66 1036.65 722.94
Standard Deviation 88.16 120.70 160.96 5.25 88.78 118.99 159.62 116.47 88.75 119.92 165.87 349.81
Intensity Change Log 2 — 0.138222 0.455438 -1.119403 — 0.155588 0.564789 0.484850 — 0.146971 0.511398 -0.101596

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000149 0.000733 0.000586 0.000159 0.000110 0.000439 0.000776 0.000158 0.000113 0.000550 0.000697 0.000159
Bud Neck 0.003516 0.008206 0.013966 0.000011 0.002013 0.008504 0.015525 0.019104 0.002120 0.008391 0.014875 0.010950
Bud Site 0.001571 0.002187 0.002978 0.000028 0.000472 0.002985 0.001726 0.002029 0.000550 0.002683 0.002248 0.001174
Cell Periphery 0.000197 0.000334 0.000128 0.000430 0.000108 0.000181 0.000094 0.000289 0.000114 0.000239 0.000108 0.000349
Cytoplasm 0.080436 0.025283 0.018684 0.000089 0.048850 0.013100 0.004885 0.102098* 0.051100 0.017705 0.010634 0.058532
Cytoplasmic Foci 0.022921 0.003742 0.000348 0.000031 0.004883 0.002747 0.000084 0.000148 0.006168 0.003123 0.000194 0.000098
Eisosomes 0.000037 0.000029 0.000021 0.001114 0.000014 0.000020 0.000029 0.000018 0.000016 0.000024 0.000025 0.000486
Endoplasmic Reticulum 0.001742 0.009847 0.004610 0.000002 0.001986 0.007418 0.004670 0.000778 0.001968 0.008336 0.004645 0.000446
Endosome 0.001590 0.001501 0.000464 0.000000 0.000511 0.001921 0.000245 0.000302 0.000587 0.001762 0.000337 0.000173
Golgi 0.000405 0.000500 0.000067 0.000005 0.000228 0.000890 0.000072 0.000068 0.000241 0.000743 0.000070 0.000041
Lipid Particles 0.001608 0.001447 0.000246 0.000126 0.001096 0.000814 0.000091 0.000029 0.001133 0.001053 0.000155 0.000070
Mitochondria 0.004890 0.002613 0.001461 0.000018 0.001249 0.005424 0.001644 0.000796 0.001508 0.004362 0.001567 0.000464
Mitotic Spindle 0.000347 0.002526 0.004682 0.000226 0.002205 0.004600 0.009989 0.002120 0.002072 0.003816 0.007778 0.001311
None 0.008486 0.012464 0.003199 0.997132* 0.006341 0.005337 0.004151 0.010491 0.006493 0.008031 0.003754 0.431869*
Nuclear Periphery 0.000521 0.001503 0.001151 0.000000 0.001770 0.002267 0.000597 0.001061 0.001681 0.001978 0.000828 0.000608
Nuclear Periphery Foci 0.001219 0.001883 0.000315 0.000002 0.000611 0.000737 0.000186 0.000030 0.000655 0.001170 0.000240 0.000018
Nucleolus 0.006408 0.009014 0.001897 0.000004 0.004140 0.007540 0.005160 0.002087 0.004301 0.008097 0.003800 0.001198
Nucleus 0.850500* 0.910748* 0.942107* 0.000014 0.921392* 0.924754* 0.948015* 0.855175* 0.916343* 0.919460* 0.945553* 0.489950*
Peroxisomes 0.000857 0.001884 0.000138 0.000608 0.000394 0.001159 0.000185 0.000024 0.000427 0.001433 0.000166 0.000274
Vacuole 0.011954 0.002710 0.002815 0.000000 0.001484 0.007194 0.001750 0.003068 0.002230 0.005499 0.002193 0.001758
Vacuole Periphery 0.000646 0.000845 0.000137 0.000000 0.000143 0.001969 0.000125 0.000125 0.000179 0.001544 0.000130 0.000072

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.44 -6.73 0.14 6.03 8.39 -2.45 -21.14 -0.97 2.08 15.97* -5.01 -24.12 -1.05 4.41 17.59*
Bud Neck -3.53 -7.51 2.73 11.88 14.81 -8.29 -14.03 -3.57 -2.28 0.66 -10.88 -17.81 -3.07 -0.99 2.72
Bud Site -1.41 -1.90 3.16 7.83 4.49 -4.67 -8.42 -2.67 1.47 2.94 -6.15 -7.83 -1.63 3.57 5.08
Cell Periphery -2.19 1.45 -1.53 -0.63 -2.07 -3.10 0.34 -2.52 -1.32 -2.61 -5.32 0.03 -3.07 -1.31 -3.09
Cytoplasm 3.80 4.45 5.62 10.58 10.51 11.05 14.61 -2.42 -4.29 -4.75 10.45 13.71 -0.31 -3.07 -3.78
Cytoplasmic Foci 3.11 3.78 3.83 4.46 3.45 2.55 5.77 6.40 5.19 1.13 3.22 7.02 7.46 6.80 1.93
Eisosomes 1.04 2.23 -2.79 -2.81 -2.83 -3.93 -14.20 -0.40 0.70 3.32 -5.45 -11.47 -2.72 -2.67 -2.63
Endoplasmic Reticulum -13.93 -8.61 6.27 19.40 19.28 -11.16 -11.96 7.09 13.50 16.27 -17.40 -15.30 11.23 21.86 24.02
Endosome 0.46 1.74 2.42 4.38 10.24 -4.77 1.63 2.06 5.64 0.96 -4.75 1.89 4.33 7.21 5.04
Golgi -0.27 1.66 2.10 3.23 12.65 -1.78 0.21 1.61 2.35 6.67 -1.99 0.88 2.12 3.13 9.11
Lipid Particles 0.28 2.45 2.66 3.98 1.34 0.88 3.11 3.57 5.77 5.88 0.23 3.32 3.77 6.39 2.93
Mitochondria 0.58 0.88 1.30 3.74 7.24 -4.23 -1.17 0.64 5.60 4.85 -3.50 -0.49 1.65 6.75 7.89
Mitotic Spindle -2.43 -4.15 0.62 2.80 4.34 -1.67 -6.36 -0.47 0.45 5.13 -1.65 -6.83 0.03 1.22 6.19
None -1.43 2.53 -430.91* -494.12* -1013.82* 1.09 3.45 -1.73 -2.18 -3.03 -1.48 4.71 -8.48 -8.45 -8.54
Nuclear Periphery -5.19 -5.81 4.59 10.11 15.05 -1.02 1.88 0.79 2.23 -1.28 -0.74 1.34 2.12 4.67 2.03
Nuclear Periphery Foci -0.23 0.75 1.19 2.49 3.29 -0.53 -1.56 4.07 2.63 2.63 -1.38 -1.19 4.11 3.51 3.25
Nucleolus -0.58 1.06 1.70 7.92 7.30 -3.30 -6.49 2.03 4.74 7.29 -4.01 -5.24 3.89 8.18 7.97
Nucleus -2.58 -3.74 34.26 171.58* 239.95* -0.42 2.74 2.79 2.89 1.90 -0.38 -0.06 9.31 9.37 9.33
Peroxisomes -1.85 2.09 0.73 2.58 -1.59 -2.31 -0.24 2.97 3.66 6.44 -3.56 0.97 1.12 4.22 0.52
Vacuole 1.75 1.64 2.18 10.09 7.77 -5.23 -2.88 -0.78 1.68 0.26 -3.51 -1.72 0.57 2.75 1.55
Vacuole Periphery -0.21 1.67 2.10 1.59 12.96 -4.34 0.06 0.71 4.39 0.71 -4.11 1.36 3.39 4.48 3.75
Standard name
Human Ortholog
Description Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Dus3

Dus3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dus3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available