Standard name | |
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Human Ortholog | |
Description | Seipin involved in lipid droplet (LD) assembly; controls lipid particle morphology, number, and size; promotes initiation of LD formation on the ER; ensures that LDs bud from the ER towards the cytosolic side of the membrane; forms a complex with Ldb16p at ER-LD contact sites, stabilizing these sites; null mutants have localized accumulation of phosphatidic acid (PA) marker proteins; BSCL2, human homolog implicated in congenital lipodystrophy, complements yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 5 | 5 | 4 | 7 | 1 | 1 | 1 | 1 | 6 | 1 | 0 | 3 | 4 | 0 | 0 | 0 | 1 | 3 | 11 | 22 | 10 | 6 | 9 |
Bud | 0 | 11 | 8 | 17 | 38 | 14 | 14 | 23 | 25 | 25 | 14 | 17 | 1 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 8 |
Bud Neck | 0 | 3 | 12 | 6 | 11 | 6 | 4 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 2 | 6 | 3 | 1 | 5 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 16 | 42 | 86 | 55 | 74 | 80 | 98 | 57 | 44 | 43 | 31 | 39 | 9 | 37 | 41 | 39 | 28 | 34 | 9 | 9 | 13 | 18 | 12 | 15 |
Endoplasmic Reticulum | 9 | 16 | 3 | 8 | 7 | 6 | 2 | 2 | 15 | 27 | 5 | 6 | 17 | 14 | 1 | 13 | 6 | 9 | 0 | 3 | 7 | 3 | 3 | 6 |
Endosome | 20 | 14 | 125 | 86 | 43 | 121 | 0 | 76 | 8 | 36 | 25 | 62 | 29 | 59 | 97 | 114 | 53 | 81 | 74 | 101 | 263 | 39 | 35 | 68 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 7 | 8 | 23 | 3 | 9 | 6 |
Mitochondria | 33 | 88 | 16 | 62 | 111 | 158 | 295 | 178 | 397 | 366 | 324 | 365 | 13 | 10 | 13 | 22 | 9 | 8 | 19 | 68 | 109 | 44 | 53 | 39 |
Nucleus | 0 | 1 | 1 | 1 | 1 | 4 | 7 | 7 | 4 | 7 | 18 | 15 | 2 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 9 | 3 | 8 |
Nuclear Periphery | 1 | 3 | 0 | 0 | 0 | 1 | 2 | 1 | 8 | 12 | 2 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 7 | 14 | 4 | 2 | 5 |
Nucleolus | 0 | 1 | 1 | 0 | 1 | 2 | 3 | 2 | 6 | 7 | 6 | 4 | 0 | 1 | 2 | 5 | 3 | 4 | 0 | 0 | 1 | 3 | 0 | 4 |
Peroxisomes | 2 | 3 | 41 | 40 | 13 | 25 | 1 | 29 | 6 | 14 | 10 | 13 | 4 | 13 | 51 | 40 | 7 | 21 | 10 | 21 | 71 | 13 | 22 | 19 |
SpindlePole | 12 | 28 | 169 | 67 | 65 | 73 | 53 | 77 | 28 | 77 | 39 | 56 | 15 | 42 | 32 | 65 | 37 | 58 | 10 | 19 | 29 | 3 | 8 | 11 |
Vac/Vac Membrane | 9 | 6 | 29 | 5 | 13 | 24 | 8 | 26 | 16 | 16 | 25 | 38 | 2 | 5 | 5 | 21 | 14 | 12 | 1 | 7 | 3 | 10 | 7 | 11 |
Unique Cell Count | 82 | 189 | 400 | 278 | 295 | 422 | 412 | 386 | 470 | 514 | 415 | 508 | 76 | 155 | 180 | 205 | 111 | 143 | 143 | 270 | 577 | 172 | 170 | 227 |
Labelled Cell Count | 103 | 224 | 496 | 351 | 386 | 517 | 495 | 483 | 559 | 641 | 504 | 631 | 96 | 190 | 244 | 323 | 161 | 230 | 143 | 270 | 577 | 172 | 170 | 227 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 3.7 | 5.7 | 5.1 | 4.7 | 4.7 | 3.6 | 4.9 | 3.9 | 4.3 | 4.3 | 4.5 | 5.4 | 5.4 | 5.4 | 5.3 | 6.7 | 6.9 | 7.5 | 6.7 | 7.7 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.4 | 1.3 | 1.4 | 1.4 | 1.2 | 1.2 | 1.0 | 0.8 | 1.0 | 1.0 | 2.0 | 1.4 | 2.2 | 2.3 | 3.0 | 2.9 | 3.9 | 2.8 | 4.1 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.28 | -0.29 | -0.67 | -0.21 | -0.56 | -0.39 | -0.42 | -0.35 | -0.08 | -0.09 | -0.07 | -0.12 | 0.24 | 0.27 | 0.39 | 0.24 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 1.3 | 1.8 | 0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7961 | 3.1249 | 3.0728 | 2.775 | 3.1181 | 2.8104 | 4.6424 | 4.5131 | 4.2958 | 3.9921 | 4.6385 | 4.3006 | 1.7858 | 2.1124 | 2.0079 | 1.8852 | 2.0548 | 1.9484 |
Actin | 0.0482 | 0.0117 | 0.0172 | 0.0074 | 0.007 | 0.006 | 0.0297 | 0.0002 | 0.0235 | 0.0107 | 0.0054 | 0.0036 | 0.0045 | 0.0031 | 0.0086 | 0.0053 | 0.0053 | 0.0047 |
Bud | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0011 | 0.0003 | 0.0014 | 0.0013 | 0.0004 | 0.0009 | 0.0008 | 0.0002 | 0.0003 | 0.0012 | 0.0002 | 0.0003 | 0.0011 | 0.0003 | 0.0005 | 0.0034 | 0.0036 | 0.0013 |
Bud Periphery | 0.0006 | 0.0002 | 0.0007 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0001 |
Bud Site | 0.0013 | 0.0007 | 0.0014 | 0.0007 | 0.0003 | 0.0002 | 0.0009 | 0.0003 | 0.0007 | 0.0005 | 0.0004 | 0.0001 | 0.0007 | 0.0009 | 0.0018 | 0.0004 | 0.0004 | 0.0002 |
Cell Periphery | 0.0006 | 0.0005 | 0.0005 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0002 |
Cytoplasm | 0.0161 | 0.022 | 0.026 | 0.0371 | 0.016 | 0.0126 | 0.0159 | 0.0238 | 0.0039 | 0.005 | 0.0172 | 0.0067 | 0.0114 | 0.0161 | 0.0175 | 0.017 | 0.024 | 0.0113 |
Cytoplasmic Foci | 0.0772 | 0.0647 | 0.0784 | 0.0963 | 0.0666 | 0.063 | 0.1029 | 0.1306 | 0.1096 | 0.1102 | 0.119 | 0.0741 | 0.0983 | 0.0955 | 0.099 | 0.1292 | 0.0795 | 0.0782 |
Eisosomes | 0.0011 | 0.0002 | 0.0005 | 0.0017 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0006 | 0.0003 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0223 | 0.0276 | 0.0341 | 0.0037 | 0.0355 | 0.0112 | 0.0369 | 0.0251 | 0.0064 | 0.0164 | 0.0191 | 0.0161 | 0.0127 | 0.034 | 0.0298 | 0.0169 | 0.0282 | 0.0159 |
Endosome | 0.0583 | 0.0992 | 0.1059 | 0.0889 | 0.1081 | 0.05 | 0.0983 | 0.0786 | 0.083 | 0.0605 | 0.0619 | 0.0497 | 0.0761 | 0.1188 | 0.1193 | 0.1023 | 0.0852 | 0.0655 |
Golgi | 0.0216 | 0.0213 | 0.0214 | 0.0249 | 0.0258 | 0.0188 | 0.0463 | 0.0222 | 0.0443 | 0.0477 | 0.0263 | 0.022 | 0.0251 | 0.0336 | 0.0344 | 0.0206 | 0.0267 | 0.0199 |
Lipid Particles | 0.4915 | 0.5856 | 0.5199 | 0.5415 | 0.4861 | 0.6927 | 0.5338 | 0.5939 | 0.612 | 0.6129 | 0.5123 | 0.6212 | 0.6298 | 0.5318 | 0.5133 | 0.5403 | 0.5587 | 0.6135 |
Mitochondria | 0.0182 | 0.0054 | 0.0214 | 0.0084 | 0.015 | 0.0162 | 0.012 | 0.0009 | 0.0081 | 0.0074 | 0.0071 | 0.0196 | 0.0035 | 0.0043 | 0.024 | 0.0233 | 0.0208 | 0.0086 |
None | 0.0856 | 0.0734 | 0.0624 | 0.068 | 0.1002 | 0.038 | 0.041 | 0.0702 | 0.0184 | 0.0113 | 0.0933 | 0.0762 | 0.0293 | 0.0317 | 0.0487 | 0.0589 | 0.0357 | 0.0464 |
Nuclear Periphery | 0.0496 | 0.0359 | 0.0516 | 0.01 | 0.0441 | 0.0236 | 0.0222 | 0.0243 | 0.0104 | 0.0189 | 0.0207 | 0.0471 | 0.0533 | 0.051 | 0.0345 | 0.0147 | 0.0495 | 0.0625 |
Nucleolus | 0.0026 | 0.0009 | 0.0021 | 0.0017 | 0.0012 | 0.0005 | 0.0013 | 0.0006 | 0.0004 | 0.0014 | 0.0004 | 0.0008 | 0.0015 | 0.0017 | 0.0015 | 0.0005 | 0.0018 | 0.0012 |
Nucleus | 0.0056 | 0.0019 | 0.0039 | 0.0015 | 0.0044 | 0.0012 | 0.0028 | 0.0004 | 0.0008 | 0.004 | 0.0012 | 0.0012 | 0.0014 | 0.0019 | 0.0014 | 0.0009 | 0.0034 | 0.002 |
Peroxisomes | 0.044 | 0.0192 | 0.0201 | 0.0697 | 0.0634 | 0.0525 | 0.034 | 0.0112 | 0.0608 | 0.0581 | 0.094 | 0.039 | 0.0245 | 0.0311 | 0.0445 | 0.0381 | 0.0528 | 0.0433 |
Punctate Nuclear | 0.0441 | 0.0133 | 0.0176 | 0.0321 | 0.0163 | 0.0054 | 0.0119 | 0.0126 | 0.0139 | 0.0274 | 0.018 | 0.013 | 0.0192 | 0.0257 | 0.0102 | 0.0204 | 0.0116 | 0.0167 |
Vacuole | 0.0057 | 0.0118 | 0.0087 | 0.0029 | 0.0046 | 0.0033 | 0.005 | 0.0025 | 0.0016 | 0.003 | 0.0013 | 0.003 | 0.0038 | 0.014 | 0.0051 | 0.0037 | 0.0072 | 0.0039 |
Vacuole Periphery | 0.0044 | 0.0044 | 0.0048 | 0.0015 | 0.0039 | 0.003 | 0.0029 | 0.0021 | 0.0013 | 0.0023 | 0.0018 | 0.0054 | 0.0031 | 0.0036 | 0.0046 | 0.0029 | 0.0049 | 0.0044 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.9312 | 4.5985 | 4.2653 | 7.1056 | 6.6695 | 12.8531 | 9.0085 | 9.0221 | 7.5761 | 12.0333 |
Translational Efficiency | 0.7199 | 1.1085 | 1.3499 | 0.6718 | 1.0312 | 0.8219 | 0.6447 | 0.4448 | 0.7903 | 0.5774 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1956 | 1569 | 1609 | 1589 | 1675 | 2110 | 2792 | 1818 | 3631 | 3679 | 4401 | 3407 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 704.56 | 757.84 | 855.17 | 807.15 | 686.36 | 826.96 | 808.70 | 826.73 | 696.16 | 797.48 | 825.69 | 817.60 |
Standard Deviation | 79.79 | 90.62 | 102.03 | 122.22 | 82.07 | 94.13 | 100.18 | 106.93 | 81.36 | 98.75 | 103.31 | 114.73 |
Intensity Change Log 2 | — | 0.105171 | 0.279489 | 0.196114 | — | 0.268852 | 0.236639 | 0.268451 | — | 0.188261 | 0.258503 | 0.232263 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001136 | 0.001918 | 0.001156 | 0.001632 | 0.000675 | 0.000959 | 0.003647 | 0.000735 | 0.000923 | 0.001368 | 0.002736 | 0.001154 |
Bud Neck | 0.001789 | 0.002817 | 0.006361 | 0.016560 | 0.000737 | 0.002024 | 0.019278 | 0.018388 | 0.001304 | 0.002362 | 0.014556 | 0.017536 |
Bud Site | 0.000353 | 0.001658 | 0.005184 | 0.031727 | 0.000390 | 0.001707 | 0.013544 | 0.033115 | 0.000370 | 0.001687 | 0.010487 | 0.032467 |
Cell Periphery | 0.000132 | 0.000080 | 0.000166 | 0.000611 | 0.000063 | 0.000146 | 0.000178 | 0.000210 | 0.000100 | 0.000118 | 0.000174 | 0.000397 |
Cytoplasm | 0.026984 | 0.028335 | 0.074243 | 0.330763* | 0.017912 | 0.029525 | 0.198852* | 0.387356* | 0.022799 | 0.029017 | 0.153295* | 0.360961* |
Cytoplasmic Foci | 0.132603 | 0.198336* | 0.071471 | 0.088875 | 0.080047 | 0.123071 | 0.149453 | 0.085974 | 0.108359 | 0.155170 | 0.120943 | 0.087327 |
Eisosomes | 0.000355 | 0.000167 | 0.000091 | 0.000113 | 0.000151 | 0.000230 | 0.000190 | 0.000101 | 0.000261 | 0.000204 | 0.000154 | 0.000107 |
Endoplasmic Reticulum | 0.004371 | 0.006804 | 0.013134 | 0.004178 | 0.004708 | 0.005176 | 0.008056 | 0.003677 | 0.004527 | 0.005870 | 0.009912 | 0.003911 |
Endosome | 0.031065 | 0.074570 | 0.071308 | 0.032546 | 0.019080 | 0.040922 | 0.065690 | 0.038685 | 0.025536 | 0.055272 | 0.067744 | 0.035822 |
Golgi | 0.082245 | 0.103305 | 0.003477 | 0.006094 | 0.048777 | 0.047705 | 0.005414 | 0.002007 | 0.066806 | 0.071417 | 0.004706 | 0.003913 |
Lipid Particles | 0.648087* | 0.460517* | 0.189816* | 0.025296 | 0.752951* | 0.666493* | 0.172615* | 0.026837 | 0.696461* | 0.578649* | 0.178904* | 0.026118 |
Mitochondria | 0.013930 | 0.020445 | 0.002759 | 0.003784 | 0.011935 | 0.014849 | 0.006147 | 0.003925 | 0.013010 | 0.017235 | 0.004909 | 0.003859 |
Mitotic Spindle | 0.004365 | 0.039573 | 0.093332 | 0.106955* | 0.007305 | 0.009529 | 0.086362 | 0.053809 | 0.005721 | 0.022342 | 0.088910 | 0.078596 |
None | 0.001156 | 0.001746 | 0.004939 | 0.007422 | 0.001099 | 0.001560 | 0.002971 | 0.005866 | 0.001130 | 0.001639 | 0.003690 | 0.006592 |
Nuclear Periphery | 0.001009 | 0.001547 | 0.032474 | 0.006067 | 0.001720 | 0.001561 | 0.009571 | 0.003543 | 0.001337 | 0.001555 | 0.017944 | 0.004720 |
Nuclear Periphery Foci | 0.006451 | 0.016619 | 0.283834* | 0.017562 | 0.018699 | 0.014136 | 0.035025 | 0.014795 | 0.012101 | 0.015195 | 0.125989 | 0.016085 |
Nucleolus | 0.000992 | 0.001632 | 0.020426 | 0.002114 | 0.001056 | 0.001932 | 0.006761 | 0.003941 | 0.001022 | 0.001804 | 0.011756 | 0.003089 |
Nucleus | 0.005498 | 0.004065 | 0.080726 | 0.153997* | 0.005449 | 0.006301 | 0.105420 | 0.180320* | 0.005476 | 0.005347 | 0.096392 | 0.168043* |
Peroxisomes | 0.032970 | 0.025901 | 0.012493 | 0.020915 | 0.024646 | 0.026272 | 0.043167 | 0.011465 | 0.029130 | 0.026114 | 0.031952 | 0.015872 |
Vacuole | 0.003674 | 0.007936 | 0.031893 | 0.139623 | 0.002180 | 0.004720 | 0.066264 | 0.122620 | 0.002985 | 0.006092 | 0.053698 | 0.130550 |
Vacuole Periphery | 0.000834 | 0.002029 | 0.000718 | 0.003166 | 0.000421 | 0.001182 | 0.001396 | 0.002630 | 0.000644 | 0.001543 | 0.001148 | 0.002880 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.47 | 0.04 | 0.37 | 2.91 | 0.30 | -1.22 | -4.67 | 0.79 | 1.64 | 4.90 | -2.26 | -4.09 | 0.93 | 3.08 | 4.58 |
Bud Neck | -2.42 | -10.79 | -12.83 | -11.76 | -7.63 | -3.73 | -21.73 | -13.01 | -11.93 | 1.67 | -3.78 | -23.23 | -18.00 | -16.66 | -1.44 |
Bud Site | -5.87 | -7.36 | -10.61 | -9.85 | -7.33 | -2.51 | -10.24 | -10.82 | -10.07 | -5.07 | -4.36 | -12.25 | -15.04 | -13.93 | -7.86 |
Cell Periphery | 1.02 | -0.75 | -2.28 | -3.21 | -2.18 | -2.32 | -9.30 | -7.24 | -0.47 | -0.01 | -0.54 | -2.37 | -3.15 | -3.00 | -2.16 |
Cytoplasm | -0.87 | -12.07 | -36.81 | -35.79 | -26.71 | -3.94 | -34.99 | -43.85 | -41.62 | -15.75 | -3.16 | -35.39 | -56.96 | -54.78 | -26.34 |
Cytoplasmic Foci | -9.61 | 11.62 | 11.18 | 20.72 | -0.16 | -7.94 | -13.71 | -1.32 | 7.06 | 13.10 | -10.66 | -3.05 | 7.59 | 18.80 | 11.93 |
Eisosomes | 5.61 | 7.74 | 7.76 | 6.33 | 0.30 | -1.87 | -1.08 | 2.83 | 3.29 | 7.20 | 1.98 | 5.73 | 8.21 | 4.48 | 6.72 |
Endoplasmic Reticulum | -2.24 | -9.02 | -0.29 | 2.30 | 10.17 | -0.43 | -3.23 | 1.51 | 1.81 | 6.02 | -1.83 | -8.05 | 0.93 | 2.83 | 10.86 |
Endosome | -12.74 | -11.67 | 2.93 | 14.95 | 14.02 | -9.22 | -17.38 | -6.26 | 2.43 | 9.67 | -14.58 | -19.57 | -2.50 | 11.83 | 16.47 |
Golgi | -3.96 | 25.16 | 25.05 | 24.91 | 0.49 | 0.44 | 18.54 | 19.81 | 20.97 | 7.02 | -1.34 | 30.82 | 31.62 | 31.74 | 4.69 |
Lipid Particles | 16.13 | 50.98* | 80.71* | 48.58* | 23.76* | 8.42 | 71.62* | 97.40* | 82.06* | 24.55* | 14.85 | 85.15 | 122.50 | 90.43* | 34.10* |
Mitochondria | -2.89 | 8.07 | 8.44 | 9.57 | 1.98 | -1.37 | 3.68 | 5.43 | 8.86 | 4.99 | -2.82 | 7.67 | 9.66 | 13.07 | 5.76 |
Mitotic Spindle | -11.18 | -17.87 | -15.03 | -7.37 | 1.25 | -1.61 | -19.30 | -11.38 | -10.62 | 5.39 | -10.31 | -26.52 | -18.86 | -12.68 | 4.18 |
None | -1.00 | -4.56 | -5.16 | -3.67 | -0.15 | -0.75 | -4.57 | -5.05 | -3.91 | -2.34 | -1.23 | -6.46 | -7.22 | -5.38 | -1.91 |
Nuclear Periphery | -1.69 | -16.74 | -11.80 | -10.25 | 12.29 | 0.41 | -12.94 | -7.88 | -9.80 | 8.22 | -1.01 | -21.13 | -14.46 | -13.97 | 14.05 |
Nuclear Periphery Foci | -6.15 | -35.01* | -6.11 | -0.46 | 32.80* | 1.99 | -7.04 | 0.86 | -1.11 | 8.11 | -2.60 | -30.92 | -3.53 | -1.17 | 28.85* |
Nucleolus | -2.06 | -13.47 | -6.93 | -4.74 | 11.87 | -1.98 | -12.31 | -8.02 | -4.84 | 4.04 | -2.69 | -17.93 | -10.44 | -6.19 | 12.19 |
Nucleus | 1.43 | -18.48 | -30.01 | -30.23 | -9.80 | -0.85 | -29.88 | -33.62 | -33.52 | -9.98 | -0.10 | -35.07 | -45.09 | -45.08 | -13.73 |
Peroxisomes | 2.76 | 10.16 | 8.67 | 5.77 | -0.20 | -0.67 | -5.20 | 7.57 | 9.02 | 13.60 | 1.78 | 0.54 | 11.80 | 10.36 | 11.60 |
Vacuole | -4.57 | -15.82 | -34.64 | -33.61 | -27.73 | -3.88 | -30.06 | -34.45 | -33.91 | -16.54 | -5.68 | -33.24 | -48.68 | -47.65 | -29.56 |
Vacuole Periphery | -4.09 | 0.91 | -1.22 | 1.34 | -1.70 | -3.22 | -4.20 | -2.68 | -0.76 | -1.00 | -4.70 | -2.13 | -2.66 | 0.23 | -1.70 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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