Standard name
Human Ortholog
Description Protein involved in microtubule-related processes; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0
Cytoplasm 0.96 0.98 1.0 0.98 0.97 0.97 0.9 0.91 0.79 0.83 0.69 0.66 0.99 0.99 1.0 0.98 0.94 0.96 0.92 0.95 0.96 0.96 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0.07 0.13 0.14 0.45 0.32 0.54 0.48 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 2 3
Bud 0 0 0 0 2 0 2 2 11 8 5 3 0 0 0 0 0 0 0 0 0 0 6 7
Bud Neck 0 0 0 0 0 1 1 1 0 2 0 1 0 1 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 12 5 1 0 2 8 2 5 1 4 0 4 8 2 13 26 21 0 0 1 0 0 0
Cytoplasm 120 315 144 129 195 295 340 289 251 234 127 131 365 498 574 393 329 377 128 305 135 255 430 456
Endoplasmic Reticulum 0 1 0 0 1 0 1 0 1 0 0 2 0 1 0 25 11 13 0 0 2 0 5 5
Endosome 0 0 0 0 1 1 1 0 0 2 0 0 1 1 0 1 2 0 1 1 0 0 4 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 3 2 0 0 1 1
Mitochondria 4 5 0 2 18 22 49 44 144 91 99 96 1 0 0 20 5 2 0 3 0 2 5 6
Nucleus 2 1 1 0 3 1 1 4 4 0 2 3 0 0 0 1 3 2 2 0 0 0 1 1
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 1 4
Vac/Vac Membrane 1 0 0 0 2 0 11 8 5 3 2 6 2 0 0 3 14 14 0 3 0 2 2 2
Unique Cell Count 125 321 144 131 202 304 379 319 319 283 183 199 368 501 575 401 350 393 140 323 142 267 473 505
Labelled Cell Count 130 334 150 132 223 322 416 350 422 344 239 243 373 509 577 456 394 429 140 323 142 267 473 505


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 6.0 6.7 6.1 5.1 5.3 5.0 5.2 4.1 4.3 4.1 4.0 7.1 6.9 6.8 6.2 7.7 7.6 7.4 8.2 8.3
Std Deviation (1e-4) 1.1 0.9 0.9 1.3 1.3 1.1 1.2 1.5 1.0 0.9 1.0 0.8 1.2 1.1 1.2 0.9 1.2 1.2 1.2 1.6 1.4
Intensity Change (Log2) – – – -0.13 -0.4 -0.34 -0.42 -0.38 -0.73 -0.63 -0.7 -0.74 0.09 0.03 0.02 -0.12 0.2 0.18 0.15 0.29 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1.6 0.9
Cytoplasm -1.5 -2.3 -2.1 -4.0 -3.8 -6.0 -5.3 -7.3 -7.8 -1.1 -0.9 -0.5 -1.7 -3.0 -2.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.1 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.7 3.3 4.5 4.7 0 0 0 0 0 0 0 2.7 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8469 1.5475 1.1238 0.4466 0.9153 1.3047 6.604 7.8947 7.4896 7.5662 7.0184 7.8837 3.4072 4.3771 3.7985 4.0203 3.4496 4.1034
Actin 0.0224 0.0005 0.0249 0.0006 0.026 0.0015 0.001 0.0024 0.0026 0.0005 0.0003 0.0014 0.026 0.0006 0.0024 0.0131 0.0005 0.0016
Bud 0.0005 0.0003 0.0039 0.0009 0.0003 0.0007 0.0013 0.0042 0.0068 0.0027 0.0004 0.001 0.0007 0.0018 0.0063 0.0005 0.0001 0.0001
Bud Neck 0.0016 0.0002 0.001 0.0006 0.0013 0.0011 0.0008 0.0004 0.0019 0.0012 0.0168 0.0015 0.0003 0.0002 0.0004 0.0022 0.0002 0.0004
Bud Periphery 0.0007 0.0001 0.0022 0.0013 0.0004 0.0009 0.0015 0.0037 0.0067 0.0051 0.0003 0.0015 0.0006 0.0008 0.0098 0.0005 0 0.0001
Bud Site 0.0026 0.0012 0.0084 0.0003 0.0015 0.0008 0.0082 0.0138 0.0243 0.0011 0.001 0.0004 0.002 0.0027 0.0051 0.002 0.0001 0.0001
Cell Periphery 0.0003 0.0002 0.0003 0.0002 0.0001 0.0001 0.0006 0.0005 0.0009 0.0003 0.0002 0.0002 0.0004 0.0005 0.0006 0.0005 0.0001 0.0002
Cytoplasm 0.7313 0.8449 0.7507 0.7337 0.6339 0.8722 0.8533 0.851 0.7849 0.7942 0.8369 0.8338 0.7088 0.7892 0.7372 0.7179 0.914 0.799
Cytoplasmic Foci 0.0229 0.0133 0.0229 0.0277 0.0533 0.0225 0.0115 0.0132 0.0197 0.0171 0.0155 0.0136 0.0104 0.0081 0.0137 0.0208 0.0098 0.0073
Eisosomes 0.0003 0.0001 0.0001 0.0001 0.0004 0 0.0001 0 0.0001 0 0 0 0.0002 0.0001 0.0001 0.0002 0 0.0001
Endoplasmic Reticulum 0.0077 0.0032 0.0043 0.0017 0.0042 0.004 0.0052 0.0012 0.0024 0.0017 0.0042 0.0025 0.0087 0.0031 0.0052 0.012 0.0021 0.0032
Endosome 0.0184 0.013 0.0274 0.024 0.0737 0.024 0.008 0.0075 0.0083 0.0172 0.0137 0.0086 0.0063 0.003 0.0074 0.0529 0.0076 0.0034
Golgi 0.003 0.0006 0.0024 0.0006 0.0111 0.0021 0.0007 0.0007 0.0009 0.0006 0.0006 0.0005 0.0017 0.0005 0.0006 0.0021 0.0005 0.0003
Lipid Particles 0.0022 0.0002 0.0025 0.0002 0.0185 0.0003 0.0006 0.0001 0.0005 0.0001 0.0001 0.0001 0.0024 0.0003 0.0007 0.0017 0.0001 0.0001
Mitochondria 0.0026 0.0003 0.0012 0.0008 0.0031 0.0018 0.0005 0.0004 0.0007 0.0005 0.0003 0.0004 0.0006 0.0003 0.0006 0.0008 0.0001 0.0001
None 0.1593 0.1146 0.1321 0.1932 0.0831 0.0617 0.0991 0.0944 0.125 0.1436 0.104 0.1292 0.2195 0.1841 0.194 0.138 0.0611 0.1816
Nuclear Periphery 0.0092 0.0007 0.0023 0.0005 0.017 0.0005 0.0009 0.0003 0.0008 0.0006 0.0005 0.0005 0.0023 0.0006 0.0051 0.0114 0.0003 0.0003
Nucleolus 0.0003 0.0001 0.0003 0.0001 0.001 0.0001 0.0002 0.0002 0.0007 0.0002 0.0001 0.0001 0.0003 0.0001 0.0003 0.0005 0 0
Nucleus 0.0051 0.0015 0.0018 0.0013 0.0022 0.0008 0.0013 0.0016 0.0042 0.0018 0.0015 0.0013 0.0015 0.001 0.0049 0.0061 0.0006 0.0005
Peroxisomes 0.0038 0.0001 0.0011 0.0004 0.0523 0.0002 0.0002 0.0005 0.0005 0.0002 0.0001 0.0001 0.0026 0.0001 0.0003 0.0006 0.0001 0.0001
Punctate Nuclear 0.0015 0.0003 0.0036 0.0003 0.0094 0.0004 0.0008 0.0006 0.0019 0.0004 0.0006 0.0007 0.0015 0.0006 0.002 0.0016 0.0001 0.0003
Vacuole 0.0035 0.0041 0.0055 0.0098 0.0048 0.0035 0.004 0.0031 0.0057 0.0098 0.0025 0.0021 0.0028 0.0021 0.003 0.0131 0.0022 0.0011
Vacuole Periphery 0.0011 0.0005 0.0009 0.0017 0.0024 0.0007 0.0003 0.0003 0.0005 0.001 0.0004 0.0003 0.0003 0.0002 0.0004 0.0016 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.0867 17.9341 23.067 33.6445 25.1441 26.7344 26.5953 26.1091 29.8129 32.4244
Translational Efficiency 0.4327 0.6646 0.4687 0.3368 0.3676 0.4558 0.462 0.4995 0.4312 0.4821

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
93 333 2305 1704 1821 2042 1853 2193 1914 2375 4158 3897

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 854.15 853.15 905.59 880.78 759.07 932.45 907.31 933.44 763.69 921.33 906.36 910.41
Standard Deviation 141.99 116.59 107.73 116.88 93.78 113.86 115.61 118.55 98.82 117.52 111.31 120.68
Intensity Change Log 2 — -0.001690 0.084369 0.044292 — 0.296793 0.257363 0.298324 — 0.146466 0.168356 0.169406

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001003 0.000363 0.000111 0.000372 0.000113 0.000517 0.000101 0.000195 0.000156 0.000496 0.000106 0.000272
Bud Neck 0.017322 0.008637 0.001821 0.005409 0.004760 0.014299 0.001823 0.002789 0.005371 0.013505 0.001822 0.003935
Bud Site 0.087735 0.044367 0.010991 0.030460 0.010097 0.032964 0.007441 0.023944 0.013869 0.034563 0.009409 0.026793
Cell Periphery 0.004314 0.000153 0.000041 0.000073 0.000122 0.000149 0.000043 0.000050 0.000326 0.000149 0.000042 0.000060
Cytoplasm 0.433382* 0.513100* 0.827054* 0.573306* 0.690682* 0.622786* 0.786044* 0.809781* 0.678180* 0.607407* 0.808778* 0.706380*
Cytoplasmic Foci 0.235121* 0.258842* 0.025583 0.032439 0.182005* 0.181607* 0.034959 0.031121 0.184586* 0.192436* 0.029762 0.031697
Eisosomes 0.000360 0.000058 0.000009 0.000023 0.000052 0.000098 0.000014 0.000010 0.000067 0.000093 0.000011 0.000015
Endoplasmic Reticulum 0.019776 0.000662 0.000373 0.000635 0.001451 0.000972 0.000288 0.000169 0.002342 0.000928 0.000336 0.000373
Endosome 0.035586 0.040708 0.001636 0.010087 0.010062 0.019764 0.002559 0.002539 0.011302 0.022701 0.002047 0.005840
Golgi 0.020967 0.009354 0.000118 0.002721 0.005398 0.012870 0.000182 0.000965 0.006155 0.012377 0.000146 0.001733
Lipid Particles 0.009922 0.001510 0.000315 0.000710 0.002364 0.001929 0.000378 0.000260 0.002732 0.001870 0.000343 0.000457
Mitochondria 0.009595 0.002779 0.000153 0.005193 0.001471 0.003344 0.000249 0.000314 0.001866 0.003265 0.000196 0.002447
Mitotic Spindle 0.004268 0.000385 0.002800 0.053779 0.000180 0.005071 0.004913 0.014605 0.000379 0.004414 0.003742 0.031734
None 0.000455 0.000435 0.002882 0.002435 0.003081 0.000883 0.003066 0.002004 0.002953 0.000820 0.002964 0.002193
Nuclear Periphery 0.000661 0.000052 0.000201 0.000379 0.000069 0.000122 0.000174 0.000129 0.000098 0.000112 0.000189 0.000238
Nuclear Periphery Foci 0.003951 0.000712 0.000963 0.000855 0.000428 0.000161 0.001514 0.000885 0.000599 0.000239 0.001208 0.000872
Nucleolus 0.001947 0.000262 0.000089 0.000720 0.000264 0.000287 0.000133 0.000362 0.000346 0.000284 0.000109 0.000519
Nucleus 0.028886 0.010001 0.054625 0.035856 0.025541 0.011664 0.045923 0.023719 0.025704 0.011430 0.050747 0.029026
Peroxisomes 0.005358 0.004403 0.000156 0.001329 0.000991 0.003587 0.000419 0.000502 0.001203 0.003702 0.000273 0.000864
Vacuole 0.065169 0.099850 0.069944 0.239231* 0.058674 0.082135 0.109560 0.084637 0.058989 0.084619 0.087599 0.152235
Vacuole Periphery 0.014224 0.003368 0.000134 0.003989 0.002194 0.004791 0.000216 0.001019 0.002778 0.004591 0.000171 0.002318

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.21 2.99 2.61 2.15 -2.32 -2.74 -0.36 -2.50 2.37 -1.95 -2.62 1.94 -1.18 2.33 -3.00
Bud Neck 1.34 2.42 2.09 4.59 -3.44 -11.15 7.66 5.57 14.08 -1.47 -9.87 7.69 4.71 14.07 -3.50
Bud Site 2.28 4.20 3.73 5.94 -4.05 -13.20 3.92 -3.77 7.88 -5.94 -10.91 4.76 -1.65 9.47 -6.74
Cell Periphery 1.34 1.38 1.38 5.95 -1.71 -2.33 10.63 10.03 11.97 -0.41 1.17 1.89 1.86 13.10 -1.56
Cytoplasm -2.51 -11.34 -3.81 -2.00 25.57 8.90 -5.00 -7.59 -16.32 -2.38 9.43 -11.43 2.68 -7.34 15.78
Cytoplasmic Foci -0.95 10.19 10.18 22.00 -0.07 0.17 36.05 36.88 37.38 1.30 -1.34 39.35 39.36 43.52 0.35
Eisosomes 3.59 4.15 4.04 5.45 -10.40 -7.55 9.74 10.67 17.87 4.54 -3.63 9.56 9.15 17.98 -4.27
Endoplasmic Reticulum 2.47 2.50 2.50 1.79 -0.08 1.60 5.37 6.14 3.65 6.03 3.00 4.51 4.74 3.34 2.09
Endosome -0.76 7.59 6.58 8.68 -7.12 -10.01 14.39 14.17 21.73 0.67 -10.66 16.44 12.08 20.40 -6.27
Golgi 2.62 5.09 4.79 3.90 -3.24 -10.38 23.58 15.74 17.22 -1.82 -8.61 20.12 14.74 16.89 -3.70
Lipid Particles 2.53 2.83 2.76 4.26 -4.02 1.32 7.74 8.16 6.71 3.45 2.55 7.91 7.71 6.77 -1.63
Mitochondria 1.40 2.11 1.58 0.11 -3.42 -3.81 4.37 4.44 7.75 -0.40 -2.42 4.89 1.49 3.81 -3.45
Mitotic Spindle 2.53 0.99 -7.46 -9.52 -8.71 -4.52 -4.48 -6.69 -2.85 -3.28 -4.31 -6.23 -11.46 -7.99 -9.23
None 0.29 -4.91 -3.10 -3.35 1.02 4.97 0.75 2.71 -2.94 2.28 5.10 0.83 2.45 -3.87 2.23
Nuclear Periphery 1.35 0.72 0.22 -14.77 -6.42 -0.97 -16.26 -9.77 -1.95 3.09 -0.29 -9.05 -9.89 -5.31 -3.04
Nuclear Periphery Foci 2.27 2.08 2.14 -0.72 0.53 4.25 -8.43 -2.03 -3.49 3.08 3.71 -5.84 -1.70 -4.63 2.55
Nucleolus 1.49 1.61 1.26 -1.54 -2.68 -0.80 8.03 -0.53 -0.20 -1.68 0.86 3.64 -0.55 -1.26 -3.18
Nucleus 1.56 -3.05 -0.67 -13.71 12.74 14.25 -16.94 -5.66 -18.90 12.76 12.77 -23.73 -7.50 -24.31 18.66
Peroxisomes 0.77 3.26 2.92 5.76 -4.49 -7.74 4.92 4.77 9.14 -0.47 -7.86 8.50 5.11 10.21 -3.26
Vacuole -3.61 -5.39 -23.54 -21.37 -26.40 -7.65 -19.58 -20.23 -14.57 0.70 -8.84 -21.44 -36.69 -29.84 -18.62
Vacuole Periphery 2.77 3.62 3.15 2.25 -4.52 -4.72 15.75 6.56 7.31 -1.85 -3.47 11.58 5.30 6.74 -4.77
Standard name
Human Ortholog
Description Protein involved in microtubule-related processes; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Ber1

Ber1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ber1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available