Standard name | |
---|---|
Human Ortholog | |
Description | Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome; plays a role in the formation of mutant huntingtin (Htt) aggregates in yeast |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 3 | 0 | 12 | 2 | 6 | 1 | 5 | 0 | 1 | 5 | 2 | 0 | 2 | 1 | 3 | 2 | 3 | 2 | 1 | 1 | 0 | 0 | 2 |
Bud | 2 | 0 | 0 | 3 | 1 | 4 | 5 | 13 | 7 | 10 | 7 | 5 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 2 |
Bud Neck | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 1 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 3 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 2 | 2 | 2 | 0 | 1 | 1 | 2 | 8 | 10 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 293 | 200 | 71 | 76 | 131 | 178 | 249 | 326 | 169 | 176 | 123 | 156 | 152 | 144 | 303 | 277 | 248 | 324 | 264 | 181 | 70 | 44 | 81 | 114 |
Endoplasmic Reticulum | 8 | 14 | 4 | 10 | 0 | 13 | 0 | 0 | 1 | 1 | 7 | 2 | 0 | 2 | 2 | 19 | 14 | 21 | 4 | 0 | 3 | 0 | 1 | 1 |
Endosome | 14 | 6 | 1 | 48 | 45 | 40 | 44 | 46 | 37 | 20 | 18 | 22 | 10 | 21 | 10 | 27 | 13 | 36 | 52 | 27 | 11 | 8 | 15 | 43 |
Golgi | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 15 | 0 | 0 | 1 | 1 | 11 | 20 | 55 | 59 | 51 | 118 | 62 | 1 | 0 | 1 | 3 | 2 | 2 | 15 | 5 | 5 | 2 | 5 | 8 |
Nucleus | 0 | 1 | 0 | 1 | 2 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 3 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 2 | 7 | 6 | 6 | 4 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 0 | 29 | 23 | 17 | 23 | 46 | 25 | 20 | 9 | 21 | 1 | 3 | 2 | 7 | 1 | 2 | 7 | 1 | 0 | 0 | 3 | 1 |
Vac/Vac Membrane | 7 | 1 | 7 | 2 | 18 | 8 | 9 | 14 | 15 | 11 | 1 | 7 | 5 | 4 | 5 | 21 | 19 | 19 | 12 | 22 | 17 | 3 | 24 | 41 |
Unique Cell Count | 331 | 217 | 78 | 164 | 203 | 254 | 332 | 464 | 265 | 267 | 234 | 254 | 166 | 167 | 314 | 327 | 281 | 382 | 371 | 245 | 120 | 63 | 140 | 231 |
Labelled Cell Count | 347 | 225 | 84 | 186 | 224 | 280 | 363 | 522 | 323 | 301 | 294 | 282 | 171 | 181 | 326 | 361 | 312 | 423 | 371 | 245 | 120 | 63 | 140 | 231 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.7 | 7.1 | 6.3 | 7.2 | 5.4 | 5.7 | 5.9 | 5.1 | 5.2 | 4.6 | 5.1 | 6.9 | 6.6 | 6.8 | 8.1 | 8.2 | 7.6 | 6.1 | 7.3 | 6.9 |
Std Deviation (1e-4) | 0.6 | 0.8 | 1.5 | 1.1 | 6.1 | 1.0 | 1.4 | 1.2 | 1.5 | 1.3 | 1.5 | 1.6 | 1.8 | 2.2 | 1.6 | 1.1 | 1.6 | 1.3 | 1.8 | 2.4 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.17 | 0.01 | -0.4 | -0.33 | -0.28 | -0.48 | -0.45 | -0.64 | -0.49 | -0.05 | -0.11 | -0.07 | 0.18 | 0.2 | 0.1 | -0.23 | 0.03 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -6.7 | -4.4 | -3.7 | -3.1 | -3.8 | -4.6 | -4.3 | -6.1 | -4.9 | 0.1 | -1.1 | 2.1 | -1.4 | -0.7 | -1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 5.1 | 4.2 | 3.4 | 3.0 | 2.5 | 3.1 | 0 | 0 | 2.2 | 0 | 2.9 | 0 | 2.2 | 0 | 2.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 3.2 | 4.6 | 4.2 | 0 | 4.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4.0 | 3.1 | 0 | 0 | 2.9 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | -1.1 | 0 | 0 | 0 | 0 | 0 | 0 | -0.8 | -0.7 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.219 | 1.3997 | 1.3301 | 1.3826 | 1.0745 | 1.0563 | 0.885 | 0.8086 | 0.8854 | 1.2633 | 1.6746 | 0.9006 | 0.4206 | 0.785 | 0.7867 | 0.8532 | 1.0433 | 0.875 |
Actin | 0.014 | 0.0055 | 0.0306 | 0.0152 | 0.0002 | 0.0032 | 0.0172 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0075 | 0.0261 | 0.0012 | 0.0047 | 0.0112 | 0.0019 | 0.0118 |
Bud | 0.0054 | 0.0033 | 0.005 | 0.0028 | 0.0003 | 0.0021 | 0.0005 | 0.0001 | 0.0004 | 0.0006 | 0 | 0.0007 | 0.0008 | 0.0012 | 0.0008 | 0.0042 | 0.001 | 0.003 |
Bud Neck | 0.0047 | 0.0008 | 0.0018 | 0.0115 | 0.0002 | 0.0018 | 0.0015 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0008 | 0.0016 | 0.0001 | 0.0003 | 0.0005 | 0.0003 | 0.0064 |
Bud Periphery | 0.0074 | 0.0059 | 0.006 | 0.0041 | 0.0002 | 0.0035 | 0.0008 | 0.0002 | 0.0009 | 0.001 | 0 | 0.0008 | 0.0011 | 0.0017 | 0.0011 | 0.003 | 0.0015 | 0.003 |
Bud Site | 0.043 | 0.0158 | 0.0231 | 0.0429 | 0.0003 | 0.0004 | 0.0146 | 0.0004 | 0.0016 | 0.0005 | 0 | 0.0002 | 0.0076 | 0.0015 | 0.0013 | 0.0016 | 0.0041 | 0.0008 |
Cell Periphery | 0.0007 | 0.0009 | 0.0004 | 0.0017 | 0.0001 | 0.0001 | 0.0009 | 0.0003 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0001 |
Cytoplasm | 0.318 | 0.4738 | 0.3425 | 0.5296 | 0.4626 | 0.5837 | 0.2241 | 0.2969 | 0.4838 | 0.6164 | 0.1028 | 0.4661 | 0.406 | 0.6193 | 0.548 | 0.5284 | 0.5138 | 0.6352 |
Cytoplasmic Foci | 0.0638 | 0.0262 | 0.0428 | 0.0325 | 0.02 | 0.0594 | 0.0479 | 0.0197 | 0.0429 | 0.0399 | 0.0038 | 0.0239 | 0.0658 | 0.0325 | 0.0471 | 0.0559 | 0.0813 | 0.0694 |
Eisosomes | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0047 | 0.0063 | 0.0042 | 0.0055 | 0.0013 | 0.0023 | 0.0117 | 0.0051 | 0.0037 | 0.0248 | 0.0025 | 0.0065 | 0.0081 | 0.0054 | 0.0074 | 0.0073 | 0.0086 | 0.0042 |
Endosome | 0.0509 | 0.0189 | 0.0462 | 0.0755 | 0.0461 | 0.0402 | 0.0543 | 0.0215 | 0.063 | 0.0982 | 0.0029 | 0.0263 | 0.1422 | 0.0359 | 0.0858 | 0.1182 | 0.1346 | 0.0793 |
Golgi | 0.0053 | 0.0014 | 0.004 | 0.0016 | 0.0037 | 0.001 | 0.0165 | 0.0009 | 0.005 | 0.0017 | 0.0001 | 0.0063 | 0.0125 | 0.0011 | 0.0171 | 0.0061 | 0.0126 | 0.0061 |
Lipid Particles | 0.0157 | 0.0018 | 0.0023 | 0.0005 | 0.0134 | 0.0011 | 0.0147 | 0.0018 | 0.0035 | 0.0021 | 0.0004 | 0.005 | 0.0205 | 0.0007 | 0.0051 | 0.0027 | 0.0249 | 0.0012 |
Mitochondria | 0.0036 | 0.0032 | 0.0107 | 0.0013 | 0.0012 | 0.0023 | 0.0057 | 0.0008 | 0.0124 | 0.0011 | 0.0001 | 0.0123 | 0.0133 | 0.0006 | 0.01 | 0.0044 | 0.0252 | 0.0042 |
None | 0.4258 | 0.4126 | 0.4595 | 0.2397 | 0.4395 | 0.289 | 0.5206 | 0.6429 | 0.364 | 0.1678 | 0.8847 | 0.426 | 0.2509 | 0.2882 | 0.2371 | 0.2209 | 0.1358 | 0.1615 |
Nuclear Periphery | 0.0096 | 0.0066 | 0.002 | 0.0037 | 0.0013 | 0.0007 | 0.0111 | 0.0012 | 0.0009 | 0.0033 | 0.0006 | 0.0011 | 0.0031 | 0.001 | 0.008 | 0.0056 | 0.0025 | 0.0013 |
Nucleolus | 0.0019 | 0.0006 | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0086 | 0.0003 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0003 | 0.0005 | 0.0012 |
Nucleus | 0.0026 | 0.0027 | 0.0022 | 0.0043 | 0.0008 | 0.0015 | 0.0213 | 0.0011 | 0.0008 | 0.0027 | 0.0002 | 0.0005 | 0.0013 | 0.001 | 0.0065 | 0.0022 | 0.0012 | 0.0016 |
Peroxisomes | 0.0075 | 0.002 | 0.0029 | 0.0004 | 0.0007 | 0.0009 | 0.0054 | 0.0003 | 0.0011 | 0.0002 | 0 | 0.0102 | 0.0178 | 0.0004 | 0.0047 | 0.0012 | 0.0367 | 0.0009 |
Punctate Nuclear | 0.0059 | 0.0016 | 0.0033 | 0.0014 | 0.0009 | 0.0012 | 0.0057 | 0.0006 | 0.0003 | 0.0024 | 0.0002 | 0.0004 | 0.0014 | 0.0006 | 0.0014 | 0.0028 | 0.0021 | 0.0016 |
Vacuole | 0.0071 | 0.0077 | 0.0085 | 0.0216 | 0.0054 | 0.0041 | 0.0142 | 0.0048 | 0.0121 | 0.0305 | 0.0012 | 0.0038 | 0.0165 | 0.0067 | 0.0103 | 0.0194 | 0.0093 | 0.0057 |
Vacuole Periphery | 0.0021 | 0.002 | 0.0014 | 0.0037 | 0.0015 | 0.0009 | 0.002 | 0.0005 | 0.0023 | 0.0054 | 0.0001 | 0.0012 | 0.0021 | 0.0006 | 0.0027 | 0.0038 | 0.002 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.4328 | 5.7988 | 8.6059 | 11.0729 | 12.6156 | 5.8349 | 9.0879 | 10.091 | 11.7742 | 12.4843 |
Translational Efficiency | 0.5102 | 0.6045 | 0.5224 | 0.6086 | 0.5896 | 0.8007 | 0.5839 | 0.4966 | 0.5942 | 0.6784 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1675 | 1062 | 127 | 454 | 2339 | 1996 | 207 | 501 | 4014 | 3058 | 334 | 955 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 697.15 | 795.80 | 1050.06 | 902.01 | 761.68 | 760.59 | 894.30 | 788.31 | 734.75 | 772.82 | 953.53 | 842.36 |
Standard Deviation | 74.53 | 106.94 | 110.27 | 111.75 | 86.21 | 98.97 | 119.50 | 83.02 | 87.53 | 103.18 | 138.54 | 113.03 |
Intensity Change Log 2 | — | 0.190937 | 0.590931 | 0.371674 | — | -0.002066 | 0.231574 | 0.049578 | — | 0.093392 | 0.414484 | 0.212485 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000153 | 0.000440 | 0.000877 | 0.023145 | 0.000218 | 0.000276 | 0.000053 | 0.001093 | 0.000191 | 0.000333 | 0.000367 | 0.011576 |
Bud Neck | 0.028564 | 0.018555 | 0.004779 | 0.011319 | 0.022990 | 0.024735 | 0.007970 | 0.020730 | 0.025316 | 0.022589 | 0.006757 | 0.016256 |
Bud Site | 0.007991 | 0.016853 | 0.015294 | 0.081468 | 0.008934 | 0.018021 | 0.003034 | 0.027991 | 0.008540 | 0.017615 | 0.007696 | 0.053414 |
Cell Periphery | 0.000112 | 0.000088 | 0.000232 | 0.000203 | 0.000093 | 0.000073 | 0.000305 | 0.000339 | 0.000101 | 0.000078 | 0.000278 | 0.000274 |
Cytoplasm | 0.283916* | 0.319639* | 0.237118* | 0.233318* | 0.249919* | 0.262925* | 0.286563* | 0.294341* | 0.264105* | 0.282621* | 0.267762* | 0.265331* |
Cytoplasmic Foci | 0.370433* | 0.302084* | 0.131212 | 0.115960 | 0.314191* | 0.275790* | 0.100190 | 0.092340 | 0.337660* | 0.284921* | 0.111986 | 0.103569 |
Eisosomes | 0.000220 | 0.000083 | 0.000106 | 0.000142 | 0.000165 | 0.000089 | 0.000281 | 0.000102 | 0.000188 | 0.000087 | 0.000214 | 0.000121 |
Endoplasmic Reticulum | 0.001719 | 0.001036 | 0.004437 | 0.001300 | 0.003183 | 0.001159 | 0.000803 | 0.001109 | 0.002572 | 0.001116 | 0.002184 | 0.001200 |
Endosome | 0.114900 | 0.117558 | 0.101025 | 0.074101 | 0.154237 | 0.147196 | 0.047096 | 0.087440 | 0.137822 | 0.136903 | 0.067602 | 0.081099 |
Golgi | 0.016844 | 0.007452 | 0.001032 | 0.001699 | 0.017689 | 0.013493 | 0.000165 | 0.004729 | 0.017337 | 0.011395 | 0.000494 | 0.003289 |
Lipid Particles | 0.022688 | 0.007097 | 0.010848 | 0.002750 | 0.022400 | 0.010300 | 0.004282 | 0.008439 | 0.022520 | 0.009188 | 0.006779 | 0.005735 |
Mitochondria | 0.007582 | 0.005886 | 0.000162 | 0.013895 | 0.006357 | 0.008889 | 0.000183 | 0.009487 | 0.006868 | 0.007846 | 0.000175 | 0.011583 |
Mitotic Spindle | 0.004485 | 0.003626 | 0.008433 | 0.038143 | 0.004216 | 0.014566 | 0.000867 | 0.018763 | 0.004328 | 0.010767 | 0.003744 | 0.027976 |
None | 0.002263 | 0.000570 | 0.000266 | 0.001194 | 0.000681 | 0.001467 | 0.004261 | 0.003285 | 0.001341 | 0.001156 | 0.002742 | 0.002291 |
Nuclear Periphery | 0.000304 | 0.000214 | 0.001410 | 0.000538 | 0.000351 | 0.000348 | 0.000832 | 0.000515 | 0.000331 | 0.000301 | 0.001052 | 0.000526 |
Nuclear Periphery Foci | 0.000780 | 0.000605 | 0.021092 | 0.001893 | 0.000559 | 0.000546 | 0.001491 | 0.001338 | 0.000651 | 0.000567 | 0.008944 | 0.001602 |
Nucleolus | 0.001703 | 0.000533 | 0.000665 | 0.000632 | 0.000732 | 0.000977 | 0.001631 | 0.002654 | 0.001137 | 0.000823 | 0.001264 | 0.001693 |
Nucleus | 0.025871 | 0.013846 | 0.032928 | 0.024932 | 0.019621 | 0.013511 | 0.269900* | 0.074524 | 0.022229 | 0.013627 | 0.179794* | 0.050948 |
Peroxisomes | 0.010694 | 0.009264 | 0.005061 | 0.060517 | 0.006912 | 0.011014 | 0.003632 | 0.031941 | 0.008490 | 0.010406 | 0.004175 | 0.045526 |
Vacuole | 0.094574 | 0.168538 | 0.421341* | 0.308002* | 0.159758 | 0.187527 | 0.265945* | 0.315040* | 0.132557 | 0.180932 | 0.325033* | 0.311694* |
Vacuole Periphery | 0.004203 | 0.006034 | 0.001682 | 0.004850 | 0.006795 | 0.007098 | 0.000516 | 0.003799 | 0.005713 | 0.006729 | 0.000960 | 0.004299 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.25 | -4.32 | -3.65 | -3.54 | -3.34 | -0.99 | 2.73 | -2.22 | -2.05 | -2.59 | -1.62 | -3.08 | -3.87 | -3.77 | -3.68 |
Bud Neck | 4.65 | 12.77 | 8.22 | 4.21 | -2.06 | -0.92 | 7.29 | 2.26 | 2.76 | -2.74 | 2.01 | 12.46 | 6.54 | 5.01 | -3.13 |
Bud Site | -5.79 | -3.24 | -7.05 | -5.49 | -4.97 | -6.42 | 3.62 | -3.95 | -1.38 | -5.03 | -8.58 | -0.77 | -7.98 | -5.23 | -7.27 |
Cell Periphery | 2.72 | -5.97 | -1.21 | -2.58 | 4.20 | 3.81 | -2.53 | -1.16 | -1.33 | 0.12 | 4.90 | -3.78 | -1.32 | -1.70 | 0.85 |
Cytoplasm | -3.76 | 2.22 | 2.98 | 5.41 | -0.33 | -1.80 | -1.17 | -2.59 | -1.53 | -0.70 | -3.27 | 0.51 | -0.13 | 1.89 | -0.52 |
Cytoplasmic Foci | 8.29 | 18.96 | 36.39 | 24.88 | 2.92 | 6.14 | 21.91 | 32.50 | 25.62 | 0.62 | 10.54 | 29.11 | 48.32 | 35.74 | 2.38 |
Eisosomes | 5.79 | 4.39 | 4.20 | -2.54 | -0.10 | 8.14 | -1.16 | 6.08 | -1.40 | 2.40 | 8.96 | 0.15 | 6.58 | -2.76 | 2.30 |
Endoplasmic Reticulum | 2.49 | -5.08 | 1.64 | -1.33 | 6.08 | 4.43 | 5.18 | 4.45 | 0.38 | -0.11 | 4.94 | 0.08 | 4.79 | -0.16 | 4.59 |
Endosome | -0.04 | 2.29 | 12.01 | 11.18 | 4.53 | 1.47 | 12.16 | 11.01 | 10.02 | -2.26 | 0.59 | 10.74 | 16.32 | 15.54 | 1.06 |
Golgi | 7.29 | 15.18 | 15.23 | 7.83 | 0.66 | 3.36 | 18.74 | 9.86 | 6.99 | -2.40 | 6.40 | 24.16 | 16.56 | 10.26 | -2.38 |
Lipid Particles | 10.69 | 5.80 | 14.88 | 6.15 | 4.87 | 9.45 | 15.23 | 7.34 | 1.99 | -1.14 | 13.70 | 14.52 | 13.67 | 4.01 | 1.54 |
Mitochondria | 1.15 | 8.43 | 0.93 | 0.20 | -2.02 | -2.34 | 12.10 | 0.25 | 1.18 | -2.32 | -0.96 | 14.17 | 0.79 | 1.20 | -3.08 |
Mitotic Spindle | 0.49 | -0.69 | -4.98 | -5.09 | -3.75 | -4.78 | -0.75 | -3.52 | -0.90 | -2.07 | -4.26 | -1.01 | -6.08 | -4.23 | -4.26 |
None | 3.68 | 4.61 | 2.15 | -1.85 | -3.20 | -1.44 | -1.41 | -2.18 | -1.27 | 0.13 | 0.39 | -0.69 | -1.30 | -1.41 | 0.00 |
Nuclear Periphery | 1.93 | -5.90 | -7.30 | -9.09 | 3.06 | 0.00 | -5.35 | -4.34 | -4.80 | 1.59 | 0.53 | -7.95 | -7.65 | -8.70 | 3.24 |
Nuclear Periphery Foci | 1.28 | -4.32 | -5.47 | -7.21 | 4.01 | -0.04 | -4.59 | -2.51 | -2.46 | -0.27 | 0.60 | -4.64 | -4.23 | -4.38 | 3.71 |
Nucleolus | 3.14 | 2.85 | 2.91 | -0.68 | 0.30 | -2.33 | -2.19 | -3.51 | -2.94 | -1.61 | 1.88 | -0.01 | -1.33 | -2.77 | -1.21 |
Nucleus | 6.93 | -2.22 | -3.19 | -6.48 | -0.15 | 5.51 | -10.51 | -9.75 | -10.87 | 7.37 | 8.92 | -9.93 | -9.94 | -12.52 | 7.02 |
Peroxisomes | 1.55 | 5.28 | -4.73 | -5.07 | -6.03 | -4.76 | 1.64 | -4.45 | -3.51 | -4.78 | -2.70 | 3.88 | -6.55 | -5.93 | -7.53 |
Vacuole | -12.91 | -15.70 | -22.61 | -17.01 | -0.23 | -4.94 | -7.84 | -15.06 | -12.76 | -1.37 | -11.69 | -14.76 | -26.04 | -20.72 | -1.64 |
Vacuole Periphery | -2.73 | 5.98 | 2.50 | 4.22 | -0.41 | -0.45 | 18.52 | 5.64 | 5.90 | -2.67 | -2.43 | 17.63 | 5.92 | 7.38 | -2.29 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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