Standard name | |
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Human Ortholog | |
Description | Dihydroorotase; catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 |
Bud | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 4 | 15 | 3 | 12 | 8 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 2 | 1 | 6 | 3 | 12 | 13 | 11 | 8 | 6 | 7 | 2 | 2 | 2 | 33 | 9 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 215 | 200 | 195 | 305 | 375 | 267 | 335 | 423 | 366 | 384 | 309 | 293 | 343 | 432 | 422 | 369 | 208 | 380 | 202 | 198 | 183 | 211 | 449 | 399 |
Endoplasmic Reticulum | 1 | 2 | 0 | 1 | 6 | 4 | 3 | 10 | 7 | 3 | 1 | 8 | 3 | 0 | 1 | 7 | 4 | 10 | 1 | 1 | 0 | 1 | 2 | 6 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 |
Golgi | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 1 | 0 | 0 | 0 | 2 | 7 | 39 | 28 | 64 | 63 | 93 | 51 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 0 | 3 | 0 | 0 | 0 |
Nucleus | 3 | 1 | 2 | 4 | 4 | 4 | 5 | 11 | 10 | 12 | 6 | 11 | 5 | 2 | 7 | 5 | 1 | 0 | 0 | 0 | 2 | 0 | 5 | 1 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 4 | 12 | 43 | 26 | 95 | 101 | 88 | 104 | 43 | 67 | 3 | 7 | 6 | 26 | 11 | 34 | 1 | 3 | 11 | 4 | 7 | 5 |
Unique Cell Count | 218 | 204 | 201 | 321 | 424 | 303 | 448 | 556 | 497 | 528 | 412 | 404 | 351 | 437 | 430 | 397 | 220 | 407 | 215 | 208 | 206 | 220 | 477 | 427 |
Labelled Cell Count | 223 | 206 | 206 | 325 | 439 | 312 | 493 | 591 | 562 | 579 | 474 | 448 | 357 | 444 | 440 | 444 | 234 | 439 | 215 | 208 | 206 | 220 | 477 | 427 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.6 | 16.8 | 13.6 | 13.4 | 12.0 | 9.6 | 9.0 | 8.9 | 7.8 | 7.7 | 7.1 | 7.1 | 18.5 | 18.3 | 17.5 | 16.1 | 17.2 | 16.9 | 14.8 | 15.2 | 15.4 |
Std Deviation (1e-4) | 2.9 | 3.6 | 2.5 | 2.7 | 2.3 | 2.3 | 2.3 | 2.1 | 2.2 | 2.1 | 2.1 | 2.0 | 3.9 | 3.8 | 4.0 | 3.7 | 3.3 | 3.7 | 3.1 | 3.3 | 3.4 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.17 | -0.49 | -0.6 | -0.61 | -0.81 | -0.82 | -0.93 | -0.92 | 0.45 | 0.43 | 0.37 | 0.25 | 0.34 | 0.32 | 0.13 | 0.16 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 2.0 | 0 |
Cytoplasm | -1.1 | -3.5 | -3.5 | -6.8 | -6.6 | -7.0 | -7.2 | -6.7 | -7.2 | 0.5 | 1.7 | 0.9 | -2.0 | -1.3 | -1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.3 | 3.2 | 5.3 | 5.1 | 7.3 | 5.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.1 | 3.6 | 3.1 | 6.3 | 5.7 | 5.6 | 6.0 | 3.7 | 5.3 | 0 | 0 | 0 | 2.4 | 1.7 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.5488 | 12.8539 | 11.7539 | 11.4504 | 11.1327 | 11.1059 | 9.0689 | 11.4092 | 10.1314 | 9.2908 | 9.0848 | 10.6281 | 8.2312 | 12.1156 | 10.4939 | 9.2341 | 10.0117 | 10.7112 |
Actin | 0.0046 | 0.0002 | 0.0015 | 0.0002 | 0.0003 | 0.0008 | 0.0101 | 0.0002 | 0.0043 | 0.0009 | 0.0076 | 0.0009 | 0.0522 | 0.0003 | 0.0211 | 0.0567 | 0.0001 | 0.0001 |
Bud | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0002 | 0.0007 | 0.0012 | 0.0016 | 0.0011 | 0 | 0.0008 | 0.0054 | 0.0001 | 0.0002 |
Bud Neck | 0.0008 | 0.0002 | 0.0004 | 0.0005 | 0.0006 | 0.0012 | 0.0011 | 0.0001 | 0.0005 | 0.0009 | 0.0013 | 0.001 | 0.0014 | 0.0001 | 0.0006 | 0.0049 | 0.0002 | 0.0005 |
Bud Periphery | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0003 | 0 | 0.0001 | 0.0006 | 0.0011 | 0.0002 | 0.0009 | 0 | 0.0004 | 0.011 | 0 | 0.0002 |
Bud Site | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0021 | 0.0001 | 0.0004 | 0.0006 | 0.0012 | 0 | 0.011 | 0.0001 | 0.0014 | 0.0075 | 0 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0004 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 |
Cytoplasm | 0.9151 | 0.9798 | 0.9385 | 0.9524 | 0.9454 | 0.9414 | 0.8954 | 0.9924 | 0.924 | 0.9061 | 0.8648 | 0.9738 | 0.7846 | 0.9931 | 0.8951 | 0.637 | 0.9929 | 0.9364 |
Cytoplasmic Foci | 0.006 | 0.0018 | 0.0039 | 0.0013 | 0.0041 | 0.0042 | 0.0183 | 0.0009 | 0.0136 | 0.0111 | 0.0104 | 0.0023 | 0.0374 | 0.0008 | 0.0081 | 0.0231 | 0.0007 | 0.0013 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0019 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0058 | 0.0009 | 0.003 | 0.0014 | 0.0028 | 0.0025 | 0.0026 | 0.0004 | 0.0027 | 0.0024 | 0.0032 | 0.0015 | 0.0041 | 0.0009 | 0.0069 | 0.0025 | 0.0006 | 0.0014 |
Endosome | 0.0168 | 0.0013 | 0.0036 | 0.0008 | 0.0032 | 0.0038 | 0.0155 | 0.0003 | 0.0142 | 0.0441 | 0.0185 | 0.0013 | 0.0364 | 0.0005 | 0.0218 | 0.1375 | 0.0006 | 0.0177 |
Golgi | 0.0013 | 0.0001 | 0.0007 | 0 | 0.0003 | 0.0002 | 0.0042 | 0 | 0.0045 | 0.003 | 0.0032 | 0.0001 | 0.01 | 0.0001 | 0.0049 | 0.0353 | 0 | 0.0005 |
Lipid Particles | 0.0007 | 0 | 0.0031 | 0 | 0 | 0 | 0.0077 | 0 | 0.007 | 0.0004 | 0.0006 | 0 | 0.0218 | 0 | 0.001 | 0.0103 | 0 | 0.0001 |
Mitochondria | 0.0013 | 0.0001 | 0.0009 | 0.0001 | 0.0016 | 0.0006 | 0.005 | 0 | 0.0013 | 0.0022 | 0.0334 | 0.0002 | 0.0021 | 0 | 0.0019 | 0.0172 | 0 | 0.0015 |
None | 0.0065 | 0.0012 | 0.0025 | 0.0014 | 0.0043 | 0.0019 | 0.0077 | 0.0007 | 0.0026 | 0.0014 | 0.0015 | 0.0028 | 0.0059 | 0.0011 | 0.0243 | 0.0006 | 0.0004 | 0.0005 |
Nuclear Periphery | 0.008 | 0.0014 | 0.0041 | 0.0043 | 0.0035 | 0.0037 | 0.0035 | 0.0005 | 0.0052 | 0.0027 | 0.0038 | 0.0016 | 0.0029 | 0.0004 | 0.0028 | 0.0026 | 0.0011 | 0.0034 |
Nucleolus | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0085 | 0 | 0.0003 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 |
Nucleus | 0.0254 | 0.0114 | 0.0329 | 0.0356 | 0.0304 | 0.0338 | 0.0137 | 0.004 | 0.0127 | 0.0131 | 0.0155 | 0.0112 | 0.0064 | 0.0023 | 0.0051 | 0.0036 | 0.0023 | 0.0165 |
Peroxisomes | 0.0004 | 0 | 0.0009 | 0 | 0.0001 | 0.0001 | 0.0042 | 0 | 0.0041 | 0.0002 | 0.0021 | 0.0002 | 0.0101 | 0 | 0.0004 | 0.0118 | 0 | 0 |
Punctate Nuclear | 0.0018 | 0.0004 | 0.0021 | 0.0009 | 0.0014 | 0.0013 | 0.003 | 0.0001 | 0.0013 | 0.0012 | 0.0007 | 0.0003 | 0.0052 | 0.0001 | 0.0011 | 0.0007 | 0.0001 | 0.0002 |
Vacuole | 0.0034 | 0.0006 | 0.0009 | 0.0006 | 0.0012 | 0.0023 | 0.0019 | 0.0001 | 0.0008 | 0.0062 | 0.0164 | 0.0007 | 0.005 | 0.0001 | 0.0014 | 0.0261 | 0.0006 | 0.0122 |
Vacuole Periphery | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0006 | 0 | 0.0003 | 0.0019 | 0.0045 | 0.0002 | 0.0009 | 0 | 0.0006 | 0.0052 | 0.0002 | 0.007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 77.591 | 61.2544 | 52.8062 | 56.7628 | 46.3806 | 49.3488 | 50.0939 | 50.1003 | 52.3039 | 59.1446 |
Translational Efficiency | 1.3146 | 1.3191 | 1.5169 | 1.2647 | 1.521 | 1.6804 | 1.5943 | 1.3327 | 1.3328 | 1.348 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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