Standard name | |
---|---|
Human Ortholog | |
Description | Coronin; cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 96 | 157 | 107 | 87 | 81 | 57 | 81 | – | 61 | 73 | 71 | 77 | 72 | 104 | 46 | 62 | 37 | 28 | 261 | 325 | 164 | 112 | 151 | 223 |
Bud | 31 | 32 | 26 | 40 | 21 | 35 | 45 | – | 41 | 39 | 49 | 40 | 18 | 19 | 9 | 6 | 6 | 3 | 11 | 11 | 4 | 3 | 5 | 8 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 12 | 0 | 0 | 2 | 1 | 0 | 6 | – | 3 | 6 | 7 | 10 | 0 | 0 | 0 | 0 | 0 | 4 | – | – | – | – | – | – |
Cell Periphery | 8 | 8 | 8 | 0 | 2 | 3 | 1 | – | 4 | 0 | 4 | 6 | 5 | 3 | 2 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 8 | 8 | 6 | 2 | 9 | 5 | – | 6 | 2 | 4 | 5 | 11 | 12 | 15 | 19 | 12 | 14 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 7 | 5 | 0 | 0 | 4 | 3 | 1 | – | 2 | 0 | 4 | 1 | 1 | 3 | 0 | 1 | 4 | 4 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 6 | 7 | 7 | 5 | 6 | 7 | 12 | – | 7 | 7 | 4 | 5 | 3 | 3 | 1 | 7 | 5 | 6 | 6 | 15 | 20 | 9 | 15 | 20 |
Golgi | 55 | 150 | 150 | 58 | 103 | 63 | 73 | – | 41 | 72 | 25 | 53 | 94 | 198 | 177 | 99 | 67 | 55 | 42 | 64 | 68 | 53 | 101 | 119 |
Mitochondria | 276 | 221 | 120 | 122 | 88 | 184 | 188 | – | 209 | 195 | 276 | 229 | 85 | 117 | 96 | 29 | 22 | 22 | 5 | 8 | 10 | 8 | 9 | 8 |
Nucleus | 1 | 1 | 1 | 0 | 0 | 0 | 0 | – | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 2 | 1 | 1 | 2 | 0 | – | 0 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 5 | 4 | 3 | 4 | 7 | 12 | 4 | – | 7 | 6 | 6 | 6 | 2 | 1 | 1 | 16 | 9 | 14 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 69 | 56 | 66 | 64 | 63 | 97 | 81 | – | 59 | 65 | 35 | 80 | 68 | 60 | 92 | 92 | 26 | 30 | 29 | 58 | 103 | 25 | 68 | 145 |
SpindlePole | 10 | 9 | 9 | 4 | 9 | 19 | 23 | – | 11 | 14 | 4 | 19 | 9 | 5 | 11 | 44 | 24 | 27 | 1 | 4 | 2 | 2 | 1 | 3 |
Vac/Vac Membrane | 2 | 4 | 9 | 0 | 4 | 2 | 0 | – | 1 | 2 | 2 | 4 | 1 | 1 | 1 | 2 | 1 | 6 | 0 | 0 | 1 | 0 | 1 | 2 |
Unique Cell Count | 339 | 393 | 308 | 214 | 226 | 307 | 322 | 295 | 307 | 322 | 340 | 213 | 305 | 285 | 276 | 161 | 170 | 360 | 494 | 379 | 218 | 360 | 539 | |
Labelled Cell Count | 582 | 664 | 516 | 393 | 392 | 494 | 520 | 454 | 485 | 493 | 536 | 369 | 526 | 451 | 381 | 218 | 216 | 360 | 494 | 379 | 218 | 360 | 539 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.3 | 20.6 | 15.1 | 15.2 | 16.3 | 14.7 | 14.6 | – | 13.2 | 14.0 | 12.3 | 13.9 | 17.6 | 18.3 | 17.6 | 24.3 | 25.1 | 23.9 | 15.7 | 16.0 | 16.9 |
Std Deviation (1e-4) | 5.7 | 7.2 | 4.3 | 3.7 | 4.6 | 5.0 | 4.0 | – | 3.9 | 3.9 | 3.6 | 4.0 | 4.6 | 4.8 | 4.5 | 7.1 | 7.8 | 7.7 | 5.1 | 4.9 | 4.3 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.12 | -0.03 | -0.04 | – | -0.19 | -0.1 | -0.29 | -0.12 | 0.23 | 0.28 | 0.22 | 0.69 | 0.73 | 0.67 | 0.06 | 0.08 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.4 | 0.3 | -4.5 | -2.6 | – | -3.9 | -3.0 | -3.5 | -3.4 | -0.2 | -0.2 | -5.2 | -3.3 | -2.6 | -4.2 |
Bud | 3.5 | 0.3 | 1.2 | 2.2 | – | 2.1 | 1.7 | 2.6 | 1.4 | 0 | -1.0 | -2.7 | -3.3 | -1.9 | -2.9 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | -1.5 | 0 | – | -1.1 | 0 | -1.2 | -0.7 | -0.2 | -1.5 | 0 | -1.3 | 0 | 0 |
Cytoplasm | 0.1 | 0 | 0.3 | -0.9 | – | -0.5 | 0 | -1.2 | -1.0 | 1.5 | 0.9 | 1.7 | 2.5 | 2.5 | 2.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.3 | 0 | 1.1 | – | 0.1 | 0 | -1.0 | -0.8 | 0 | 0 | 0 | 0.2 | 0 | 0 |
Golgi | -5.0 | -0.7 | -7.3 | -6.8 | – | -9.2 | -6.5 | -11.5 | -9.1 | -1.0 | 4.1 | 3.3 | -3.1 | -1.5 | -3.5 |
Mitochondria | 4.1 | 0 | 5.2 | 4.9 | – | 7.9 | 6.1 | 12.1 | 7.2 | 0.2 | -0.2 | -1.3 | -7.9 | -5.7 | -6.0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 2.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 4.1 |
Peroxisomes | 2.2 | 1.7 | 2.9 | 1.1 | – | -0.4 | -0.1 | -3.6 | 0.6 | 2.7 | -0.5 | 3.0 | 3.2 | -1.4 | -1.0 |
SpindlePole | -0.8 | 0.7 | 1.9 | 2.4 | – | 0.6 | 1.1 | -1.5 | 1.7 | 0.8 | -1.1 | 0.6 | 5.5 | 4.8 | 5.1 |
Vacuole | 0 | -0.9 | -2.1 | 0 | – | 0 | -2.1 | -2.2 | -1.6 | 0 | 0 | 0 | -2.0 | 0 | 0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.6831 | 11.7015 | 11.7603 | 11.4358 | 10.7868 | 11.3371 | 15.1814 | 11.7761 | 12.9436 | 11.7146 | 11.823 | 13.1561 | 21.1579 | 14.145 | 17.1831 | 16.9252 | 17.0102 | 16.1521 |
Actin | 0.5719 | 0.7841 | 0.8406 | 0.6969 | 0.7873 | 0.8516 | 0.6872 | 0.7486 | 0.7962 | 0.6026 | 0.7483 | 0.8664 | 0.7873 | 0.7181 | 0.8472 | 0.8231 | 0.8025 | 0.7875 |
Bud | 0.0006 | 0.0014 | 0.0022 | 0.0005 | 0.0008 | 0.0002 | 0.0009 | 0.002 | 0.0013 | 0.0078 | 0.0042 | 0.0002 | 0.0002 | 0.002 | 0.0017 | 0.002 | 0.0091 | 0.0002 |
Bud Neck | 0.0088 | 0.001 | 0.0022 | 0.0013 | 0.0009 | 0.0006 | 0.0037 | 0.0008 | 0.0011 | 0.0127 | 0.0019 | 0.0008 | 0.0006 | 0.0006 | 0.0013 | 0.001 | 0.0005 | 0.0009 |
Bud Periphery | 0.0008 | 0.0011 | 0.0026 | 0.0004 | 0.0008 | 0.0003 | 0.0009 | 0.0013 | 0.0013 | 0.0095 | 0.0064 | 0.0003 | 0.0002 | 0.0013 | 0.0028 | 0.0019 | 0.0061 | 0.0003 |
Bud Site | 0.0064 | 0.0073 | 0.008 | 0.0011 | 0.0086 | 0.0006 | 0.0142 | 0.0201 | 0.0046 | 0.0235 | 0.0042 | 0.0004 | 0.0007 | 0.0033 | 0.005 | 0.0018 | 0.0091 | 0.0004 |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 |
Cytoplasm | 0.0042 | 0.0024 | 0.0004 | 0.0003 | 0.006 | 0.0001 | 0.0023 | 0.0086 | 0.0009 | 0.016 | 0.0008 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.001 | 0.0001 |
Cytoplasmic Foci | 0.0481 | 0.0415 | 0.0158 | 0.0444 | 0.0245 | 0.0054 | 0.029 | 0.0296 | 0.0307 | 0.0208 | 0.0149 | 0.018 | 0.0116 | 0.0322 | 0.0125 | 0.01 | 0.0148 | 0.0071 |
Eisosomes | 0.001 | 0.0006 | 0.0004 | 0.0004 | 0.0005 | 0.0011 | 0.0012 | 0.0003 | 0.0004 | 0.0006 | 0.0006 | 0.0007 | 0.0008 | 0.0003 | 0.0011 | 0.0004 | 0.0004 | 0.0006 |
Endoplasmic Reticulum | 0.0025 | 0.0001 | 0.0005 | 0.0002 | 0.0014 | 0.0002 | 0.0015 | 0.0001 | 0.0002 | 0.0043 | 0.0024 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Endosome | 0.0326 | 0.0074 | 0.0087 | 0.0239 | 0.0271 | 0.003 | 0.0158 | 0.0049 | 0.0069 | 0.0194 | 0.0263 | 0.0016 | 0.0032 | 0.0063 | 0.0026 | 0.0042 | 0.0025 | 0.0037 |
Golgi | 0.0514 | 0.0411 | 0.0309 | 0.0577 | 0.0519 | 0.0182 | 0.0652 | 0.0737 | 0.0375 | 0.059 | 0.1179 | 0.0212 | 0.1012 | 0.122 | 0.0435 | 0.0923 | 0.0917 | 0.1189 |
Lipid Particles | 0.0525 | 0.0064 | 0.0113 | 0.015 | 0.0191 | 0.0121 | 0.021 | 0.0079 | 0.0138 | 0.0067 | 0.0183 | 0.0083 | 0.0046 | 0.0118 | 0.0059 | 0.0013 | 0.0056 | 0.0115 |
Mitochondria | 0.0163 | 0.0144 | 0.0103 | 0.0318 | 0.0095 | 0.0311 | 0.0459 | 0.0102 | 0.0139 | 0.0695 | 0.0177 | 0.0283 | 0.0201 | 0.0047 | 0.0075 | 0.0074 | 0.0048 | 0.0142 |
None | 0.0187 | 0.0008 | 0.0001 | 0.0001 | 0.0021 | 0.0014 | 0.003 | 0 | 0.0004 | 0.0043 | 0.0006 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Nuclear Periphery | 0.0111 | 0 | 0.0005 | 0.0002 | 0.0009 | 0.0003 | 0.0063 | 0 | 0.0006 | 0.0194 | 0.0076 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Nucleolus | 0.0022 | 0.0001 | 0.0001 | 0 | 0.002 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0015 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 |
Nucleus | 0.0046 | 0.0001 | 0.0002 | 0 | 0.0086 | 0.0001 | 0.0028 | 0 | 0.0004 | 0.0216 | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 |
Peroxisomes | 0.1505 | 0.0888 | 0.0643 | 0.124 | 0.0438 | 0.0722 | 0.0943 | 0.0907 | 0.0836 | 0.0832 | 0.0202 | 0.0535 | 0.0693 | 0.0964 | 0.0679 | 0.0538 | 0.049 | 0.054 |
Punctate Nuclear | 0.0119 | 0.0007 | 0.0003 | 0.0004 | 0.0015 | 0.0007 | 0.002 | 0.0004 | 0.0054 | 0.0107 | 0.0027 | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 |
Vacuole | 0.002 | 0.0002 | 0.0003 | 0.0005 | 0.002 | 0.0001 | 0.001 | 0.0004 | 0.0001 | 0.0041 | 0.0018 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0001 |
Vacuole Periphery | 0.0016 | 0.0002 | 0.0003 | 0.0007 | 0.0006 | 0.0005 | 0.0009 | 0.0001 | 0.0001 | 0.0021 | 0.0016 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 90.258 | 67.0695 | 84.1948 | 82.5586 | 82.2821 | 71.6027 | 79.7961 | 87.5821 | 93.1409 | 87.6638 |
Translational Efficiency | 1.6957 | 2.0278 | 1.5466 | 1.8815 | 1.8612 | 2.2923 | 1.5987 | 1.717 | 1.4459 | 1.6 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1714 | 1893 | 456 | 1491 | 2226 | 1977 | 274 | 2010 | 3940 | 3870 | 730 | 3501 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 969.79 | 1323.45 | 1835.38 | 1519.81 | 1282.71 | 1343.49 | 1701.96 | 1367.77 | 1146.58 | 1333.69 | 1785.30 | 1432.52 |
Standard Deviation | 156.86 | 383.50 | 398.83 | 313.37 | 282.65 | 369.56 | 299.04 | 288.37 | 282.68 | 376.58 | 370.27 | 308.57 |
Intensity Change Log 2 | — | 0.448559 | 0.920335 | 0.648147 | — | 0.066791 | 0.408002 | 0.092631 | — | 0.243658 | 0.651138 | 0.358334 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.588707* | 0.645349* | 0.713891* | 0.824869* | 0.708079* | 0.754959* | 0.741341* | 0.539619* | 0.656149* | 0.701344* | 0.724194* | 0.661100* |
Bud Neck | 0.001028 | 0.001843 | 0.013383 | 0.003274 | 0.001193 | 0.001460 | 0.012818 | 0.003753 | 0.001121 | 0.001647 | 0.013171 | 0.003549 |
Bud Site | 0.001922 | 0.003244 | 0.018138 | 0.011879 | 0.002221 | 0.002315 | 0.032567 | 0.010758 | 0.002091 | 0.002769 | 0.023554 | 0.011235 |
Cell Periphery | 0.000143 | 0.000159 | 0.000415 | 0.000213 | 0.000097 | 0.000097 | 0.000281 | 0.000404 | 0.000117 | 0.000127 | 0.000365 | 0.000323 |
Cytoplasm | 0.001644 | 0.001516 | 0.000819 | 0.001799 | 0.001118 | 0.000971 | 0.002385 | 0.002748 | 0.001347 | 0.001238 | 0.001407 | 0.002344 |
Cytoplasmic Foci | 0.019351 | 0.017911 | 0.028961 | 0.011381 | 0.012489 | 0.011574 | 0.039812 | 0.033389 | 0.015474 | 0.014674 | 0.033034 | 0.024016 |
Eisosomes | 0.006035 | 0.008410 | 0.004759 | 0.004834 | 0.009461 | 0.008041 | 0.004451 | 0.002282 | 0.007971 | 0.008222 | 0.004644 | 0.003369 |
Endoplasmic Reticulum | 0.000334 | 0.000550 | 0.002454 | 0.000841 | 0.000309 | 0.000398 | 0.000986 | 0.001155 | 0.000320 | 0.000473 | 0.001903 | 0.001022 |
Endosome | 0.012433 | 0.017688 | 0.027075 | 0.010784 | 0.006806 | 0.008728 | 0.024738 | 0.051663 | 0.009254 | 0.013111 | 0.026198 | 0.034254 |
Golgi | 0.119449* | 0.112079* | 0.054198 | 0.041059 | 0.092696 | 0.067920 | 0.039454 | 0.096904 | 0.104334 | 0.089520 | 0.048664 | 0.073121 |
Lipid Particles | 0.053760 | 0.087563 | 0.089625 | 0.019818 | 0.055146 | 0.057342 | 0.025516 | 0.166316* | 0.054543 | 0.072124 | 0.065562 | 0.103925* |
Mitochondria | 0.024847 | 0.017178 | 0.006748 | 0.024735 | 0.019648 | 0.011725 | 0.017579 | 0.020594 | 0.021910 | 0.014392 | 0.010814 | 0.022358 |
Mitotic Spindle | 0.000487 | 0.000361 | 0.004792 | 0.002850 | 0.000837 | 0.000330 | 0.004498 | 0.003462 | 0.000685 | 0.000345 | 0.004682 | 0.003201 |
None | 0.000239 | 0.000197 | 0.000114 | 0.000139 | 0.000201 | 0.000190 | 0.000126 | 0.000276 | 0.000218 | 0.000194 | 0.000119 | 0.000218 |
Nuclear Periphery | 0.000025 | 0.000054 | 0.000428 | 0.000051 | 0.000039 | 0.000035 | 0.000175 | 0.000105 | 0.000033 | 0.000044 | 0.000333 | 0.000082 |
Nuclear Periphery Foci | 0.000029 | 0.000043 | 0.000469 | 0.000059 | 0.000013 | 0.000021 | 0.000424 | 0.000394 | 0.000020 | 0.000032 | 0.000452 | 0.000252 |
Nucleolus | 0.000021 | 0.000030 | 0.000036 | 0.000044 | 0.000017 | 0.000021 | 0.000041 | 0.000078 | 0.000019 | 0.000025 | 0.000038 | 0.000063 |
Nucleus | 0.000055 | 0.000029 | 0.000094 | 0.000109 | 0.000020 | 0.000031 | 0.000047 | 0.000141 | 0.000035 | 0.000030 | 0.000076 | 0.000128 |
Peroxisomes | 0.168903* | 0.085165 | 0.025536 | 0.038844 | 0.089238 | 0.073570 | 0.045041 | 0.063343 | 0.123894* | 0.079242 | 0.032857 | 0.052910 |
Vacuole | 0.000341 | 0.000336 | 0.007081 | 0.001528 | 0.000182 | 0.000137 | 0.007095 | 0.001758 | 0.000251 | 0.000235 | 0.007086 | 0.001660 |
Vacuole Periphery | 0.000247 | 0.000293 | 0.000982 | 0.000891 | 0.000188 | 0.000136 | 0.000627 | 0.000856 | 0.000214 | 0.000213 | 0.000849 | 0.000871 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.04 | -6.81 | -21.40 | -16.82 | -6.56 | -5.05 | -1.45 | 15.47* | 19.84* | 9.15 | -6.18 | -4.79 | -0.48 | 5.05 | 4.34 |
Bud Neck | -3.98 | -8.95 | -6.58 | -4.10 | 7.18 | -1.13 | -5.92 | -6.90 | -5.57 | 4.56 | -3.37 | -10.64 | -9.42 | -6.89 | 8.35 |
Bud Site | -2.09 | -6.09 | -6.43 | -5.33 | 2.08 | -0.20 | -5.74 | -7.16 | -7.10 | 4.09 | -1.63 | -8.25 | -9.63 | -8.66 | 4.56 |
Cell Periphery | -0.44 | -2.80 | -3.12 | -1.52 | 1.96 | -0.00 | -4.79 | -4.20 | -4.21 | -1.46 | -0.56 | -3.97 | -4.84 | -4.31 | 0.57 |
Cytoplasm | 0.61 | 2.82 | -1.04 | -1.29 | -2.47 | 1.28 | -0.94 | -5.03 | -5.54 | -0.05 | 0.93 | -0.38 | -4.33 | -4.66 | -1.29 |
Cytoplasmic Foci | 0.87 | -2.37 | 4.03 | 3.74 | 3.93 | 0.70 | -3.90 | -9.98 | -10.01 | 0.90 | 0.78 | -4.71 | -6.06 | -6.67 | 2.42 |
Eisosomes | -2.76 | 1.75 | 1.77 | 4.27 | -0.13 | 1.69 | 5.25 | 11.51 | 9.79 | 2.88 | -0.41 | 5.57 | 10.01 | 9.76 | 2.57 |
Endoplasmic Reticulum | -4.08 | -6.83 | -4.11 | -2.36 | 4.88 | -2.36 | -5.12 | -7.68 | -6.75 | -1.04 | -4.80 | -7.86 | -8.52 | -6.61 | 4.06 |
Endosome | -3.33 | -4.28 | 1.13 | 4.30 | 4.73 | -1.98 | -4.19 | -16.73 | -15.66 | -5.37 | -4.27 | -6.39 | -14.77 | -11.99 | -2.58 |
Golgi | 1.23 | 9.79 | 14.65 | 14.20 | 2.22 | 5.48 | 7.32 | -0.83 | -6.41 | -7.90 | 4.00 | 11.58 | 8.56 | 4.78 | -5.31 |
Lipid Particles | -6.69 | -4.04 | 9.43 | 14.99 | 8.15 | -0.53 | 5.41 | -16.91 | -16.31 | -18.61 | -5.37 | -1.84 | -11.61 | -7.05 | -5.70 |
Mitochondria | 2.98 | 7.48 | 0.03 | -2.70 | -6.77 | 4.16 | 0.48 | -0.40 | -3.87 | -0.67 | 4.81 | 5.19 | -0.24 | -4.47 | -5.01 |
Mitotic Spindle | 2.08 | -2.78 | -3.36 | -3.53 | 1.23 | 1.27 | -2.44 | -3.27 | -4.35 | 0.64 | 1.50 | -3.53 | -4.58 | -5.59 | 1.28 |
None | 1.98 | 5.84 | 2.51 | 1.45 | -0.96 | 0.01 | 3.47 | -1.25 | -0.97 | -2.27 | 0.61 | 7.09 | -0.22 | -0.55 | -2.55 |
Nuclear Periphery | -6.25 | -5.86 | -4.70 | 0.29 | 5.45 | 1.63 | -3.32 | -6.29 | -6.69 | 1.65 | -4.23 | -6.55 | -7.60 | -5.58 | 5.42 |
Nuclear Periphery Foci | -1.10 | -3.70 | -1.84 | -1.11 | 3.44 | -1.63 | -1.96 | -2.80 | -2.74 | 0.13 | -1.92 | -4.04 | -2.96 | -2.81 | 1.61 |
Nucleolus | -1.97 | -2.24 | -2.28 | -1.50 | -0.62 | -0.61 | -1.90 | -3.79 | -3.15 | -1.92 | -1.52 | -3.10 | -4.41 | -3.50 | -2.13 |
Nucleus | 0.84 | -0.85 | -1.40 | -2.05 | -0.35 | -1.02 | -4.71 | -2.34 | -2.02 | -1.84 | 0.21 | -1.30 | -2.68 | -2.86 | -1.05 |
Peroxisomes | 12.01 | 20.43 | 19.79 | 9.22 | -2.61 | 3.08 | 5.77 | 5.27 | 2.16 | -2.47 | 10.54 | 18.68 | 17.57 | 7.58 | -4.74 |
Vacuole | 0.02 | -3.64 | -2.52 | -2.68 | 2.98 | 0.77 | -2.76 | -4.67 | -4.85 | 2.10 | 0.19 | -4.60 | -5.04 | -5.28 | 3.63 |
Vacuole Periphery | -0.56 | -3.88 | -1.99 | -1.88 | 0.27 | 1.81 | -2.60 | -5.41 | -5.93 | -1.14 | 0.03 | -4.85 | -4.24 | -4.34 | -0.11 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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