Standard name
Human Ortholog
Description Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD3 has a paralog, IMD4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.06 0.08 0.09 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 1.0 1.0 0.99 0.96 0.9 0.82 0.89 0.8 0.85 0.85 0.97 0.99 0.98 1.0 0.99 0.99 0.99 0.98 0.96 0.95 0.96 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0.14 0.05 0.09 0.09 0.08 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 4 11 11 15 3 9 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 1 1 0 0 4 1 2 6 0 2 1 1 0 1 0 0 0 0 0 1 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 2 3 5 16 31 52 32 34 15 9 6 9 3 8 4 2 0 0 0 0 0 0
Cytoplasm 63 104 216 185 332 431 442 545 331 262 143 172 195 517 430 211 154 107 66 111 200 140 351 317
Endoplasmic Reticulum 0 1 1 1 4 10 43 90 18 29 16 16 5 5 5 5 8 5 0 0 1 1 5 7
Endosome 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 1 2 4 15 4 12 0 0 2 1 1 0 0 0 0 1 0 1
Nucleus 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 0 1 10 1 4 1 1 2 3 1 0 1 0 0 0 1 0 1 3
Unique Cell Count 63 106 217 185 336 448 493 665 372 328 169 202 202 524 437 212 155 108 67 113 208 148 365 341
Labelled Cell Count 63 107 220 190 343 459 526 715 399 370 183 221 209 535 441 226 168 115 67 113 208 148 365 341


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 59.7 70.5 55.5 58.2 52.4 42.1 36.3 33.1 28.6 25.2 27.0 24.5 92.9 89.1 84.2 63.6 76.9 89.5 73.2 70.6 68.7
Std Deviation (1e-4) 10.0 12.4 12.6 15.7 10.7 9.0 8.9 8.7 7.2 6.8 6.9 7.2 22.0 19.1 23.7 22.6 29.0 32.5 15.8 15.9 19.6
Intensity Change (Log2) – – – 0.07 -0.08 -0.4 -0.61 -0.75 -0.96 -1.14 -1.04 -1.18 0.74 0.68 0.6 0.2 0.47 0.69 0.4 0.35 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 3.2 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.0 3.1 3.7 3.9 4.3 3.8 2.3 0 0 0 0 0 0
Cytoplasm 0.9 -0.9 -2.5 -4.7 -6.5 -4.8 -6.8 -5.7 -5.6 -2.2 -1.0 -1.2 0 -0.2 -0.5
Endoplasmic Reticulum 0 0 0 4.2 5.5 2.9 4.2 4.3 3.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.2 0 3.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 64.6397 86.5237 75.2012 74.9448 63.9566 77.331 57.6094 74.6557 71.046 65.9022 43.6942 74.2644 67.157 84.6309 74.0498 81.4252 60.5429 76.8047
Actin 0.0171 0.0001 0.0151 0.0002 0.002 0.0039 0.0175 0.0001 0.0117 0.0001 0.0613 0.0001 0.0035 0.0002 0.0118 0.0001 0.0034 0.0004
Bud 0.0013 0.0001 0.0005 0.0005 0.0005 0.0002 0.0002 0.0012 0.0002 0.0001 0.0008 0 0.0012 0.0001 0.0005 0.0001 0.0012 0.0002
Bud Neck 0.0007 0.0003 0.0005 0.0008 0.0004 0.0009 0.0007 0.0001 0.0002 0.0003 0.0004 0.0005 0.0006 0.0001 0.0002 0.0002 0.0005 0.0006
Bud Periphery 0.0016 0 0.0007 0.0001 0.0007 0.0002 0.0001 0.0006 0.0001 0 0.001 0 0.0007 0 0.0003 0 0.001 0
Bud Site 0.0031 0.0001 0.0017 0.0001 0.0002 0.0001 0.0004 0.0002 0.0014 0 0.002 0 0.0023 0 0.0003 0 0.0019 0
Cell Periphery 0.0018 0.0004 0.0004 0.0004 0.0009 0.0001 0.0002 0.0002 0.0001 0 0.0004 0 0.0003 0 0.0003 0 0.0004 0
Cytoplasm 0.8558 0.9823 0.9262 0.9234 0.8549 0.9134 0.9131 0.9924 0.9774 0.9942 0.6738 0.9902 0.9023 0.9975 0.9412 0.9951 0.813 0.9829
Cytoplasmic Foci 0.002 0.0002 0.0079 0.0011 0.0024 0.0018 0.0134 0.0003 0.0019 0.0008 0.0347 0.0018 0.0105 0.0002 0.007 0.0005 0.0733 0.0021
Eisosomes 0.0002 0 0.0002 0 0.0008 0 0.0001 0 0.0001 0 0.0011 0 0.0001 0 0 0 0.0003 0
Endoplasmic Reticulum 0.0112 0.0041 0.0044 0.003 0.0007 0.0162 0.011 0.0001 0.0006 0.0003 0.0013 0.002 0.0023 0.0005 0.0017 0.0002 0.0041 0.0036
Endosome 0.0045 0.0003 0.0051 0.0037 0.0031 0.0192 0.0069 0.0001 0.0008 0.0005 0.0097 0.0024 0.0106 0.0001 0.0014 0.0003 0.0039 0.0042
Golgi 0.0011 0 0.0015 0.0003 0.0004 0.0022 0.0039 0 0.0013 0 0.015 0.0001 0.0036 0 0.0005 0 0.0011 0.0002
Lipid Particles 0.0015 0 0.0023 0.0001 0.0009 0.0007 0.0033 0 0.0001 0 0.0299 0 0.0087 0 0.0009 0 0.019 0
Mitochondria 0.0059 0 0.0071 0.0001 0.0681 0.0035 0.0008 0.0001 0.0003 0 0.1012 0 0.0119 0 0.0022 0 0.0106 0.0003
None 0.0013 0.0001 0.0074 0.0001 0.0031 0.0005 0.0037 0.0001 0.0013 0.0001 0.0226 0.0001 0.0013 0 0.0029 0.0001 0.0271 0.0002
Nuclear Periphery 0.0315 0.0019 0.004 0.006 0.0041 0.0169 0.0115 0.0002 0.0005 0.0006 0.0043 0.001 0.0042 0.0003 0.0024 0.0011 0.0051 0.002
Nucleolus 0.0005 0 0.0004 0 0.0026 0.0001 0.0002 0 0 0 0.0054 0 0.0011 0 0.0034 0 0.0047 0
Nucleus 0.0154 0.0012 0.0029 0.0036 0.0038 0.0042 0.0058 0.0005 0.0006 0.0009 0.0038 0.0006 0.0047 0.0004 0.0031 0.0011 0.0047 0.0009
Peroxisomes 0.0013 0 0.0004 0 0.0003 0.0001 0.0028 0 0.0005 0 0.0128 0 0.0032 0 0.0009 0 0.004 0
Punctate Nuclear 0.0003 0 0.0013 0 0.0001 0.0007 0.0021 0 0.0002 0 0.0023 0 0.0028 0 0.0016 0 0.0145 0
Vacuole 0.0307 0.0075 0.0082 0.043 0.0214 0.011 0.0015 0.0033 0.0005 0.0014 0.0069 0.0008 0.0143 0.0004 0.0082 0.0009 0.0038 0.0015
Vacuole Periphery 0.0113 0.0011 0.0021 0.0133 0.0287 0.0041 0.0007 0.0006 0.0001 0.0004 0.0093 0.0003 0.01 0.0001 0.0091 0.0003 0.0026 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 328.8454 300.5561 356.1891 309.375 291.6738 256.0558 281.8455 281.7539 269.7833 274.2061
Translational Efficiency 0.872 0.9802 0.8625 0.8731 0.8995 1.0429 0.962 0.8061 0.8196 0.8373

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1710 1122 1105 1051 1061 1963 165 1004 2771 3085 1270 2055

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3563.98 4421.69 4970.64 4478.90 4365.14 4631.41 5546.97 4577.77 3870.74 4555.14 5045.52 4527.20
Standard Deviation 630.87 1298.91 934.18 1137.18 832.30 1034.79 867.85 1051.41 813.95 1142.43 945.89 1097.23
Intensity Change Log 2 — 0.311109 0.479942 0.329655 — 0.085424 0.345672 0.068617 — 0.191251 0.407574 0.191820

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000032 0.000162 0.000126 0.000355 0.000064 0.000090 0.000274 0.000371 0.000045 0.000116 0.000145 0.000363
Bud Neck 0.000142 0.000465 0.002397 0.002351 0.000487 0.000317 0.006964 0.001905 0.000274 0.000371 0.002991 0.002133
Bud Site 0.000803 0.002349 0.003197 0.014619 0.001356 0.002398 0.004648 0.014446 0.001015 0.002381 0.003385 0.014535
Cell Periphery 0.001821 0.005292 0.013485 0.013927 0.008445 0.002179 0.024381 0.023841 0.004357 0.003311 0.014901 0.018770
Cytoplasm 0.953179* 0.893348* 0.782498* 0.746974* 0.863808* 0.926604* 0.492736* 0.731382* 0.918960* 0.914509* 0.744851* 0.739356*
Cytoplasmic Foci 0.003749 0.004775 0.014288 0.007132 0.006107 0.004928 0.036887 0.005998 0.004652 0.004872 0.017224 0.006578
Eisosomes 0.000000 0.000002 0.000003 0.000007 0.000001 0.000001 0.000010 0.000005 0.000001 0.000001 0.000004 0.000006
Endoplasmic Reticulum 0.000068 0.000213 0.000193 0.000319 0.000160 0.000139 0.000114 0.000198 0.000103 0.000166 0.000183 0.000260
Endosome 0.000151 0.000221 0.000402 0.002567 0.000336 0.000820 0.000726 0.001139 0.000222 0.000602 0.000444 0.001870
Golgi 0.008957 0.011206 0.016821 0.011875 0.028160 0.012865 0.025585 0.009309 0.016309 0.012262 0.017959 0.010621
Lipid Particles 0.001506 0.005270 0.010335 0.013670 0.009430 0.002638 0.031939 0.020365 0.004540 0.003595 0.013142 0.016941
Mitochondria 0.000047 0.000244 0.000132 0.002687 0.000070 0.000190 0.000097 0.000676 0.000056 0.000210 0.000127 0.001705
Mitotic Spindle 0.000293 0.000245 0.001271 0.002900 0.001600 0.001179 0.001628 0.000868 0.000794 0.000839 0.001317 0.001908
None 0.003534 0.007478 0.002732 0.001859 0.003149 0.003703 0.001338 0.001216 0.003387 0.005076 0.002551 0.001544
Nuclear Periphery 0.000064 0.000208 0.001026 0.000306 0.000411 0.000170 0.002327 0.000244 0.000196 0.000184 0.001195 0.000275
Nuclear Periphery Foci 0.002523 0.005225 0.008705 0.004270 0.010956 0.005244 0.015697 0.004050 0.005752 0.005237 0.009613 0.004163
Nucleolus 0.000026 0.000439 0.000085 0.000341 0.000068 0.000072 0.000131 0.000216 0.000042 0.000205 0.000091 0.000280
Nucleus 0.013180 0.024732 0.041131 0.017887 0.017133 0.013300 0.073462 0.017530 0.014693 0.017457 0.045332 0.017713
Peroxisomes 0.000004 0.000014 0.000013 0.000231 0.000010 0.000081 0.000022 0.000083 0.000006 0.000057 0.000014 0.000159
Vacuole 0.005512 0.032909 0.085126 0.145865 0.035944 0.016393 0.264176* 0.159921 0.017164 0.022400 0.108388 0.152732
Vacuole Periphery 0.004410 0.005204 0.016034 0.009858 0.012304 0.006690 0.016857 0.006237 0.007432 0.006150 0.016141 0.008089

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.58 -10.15 -4.23 -1.24 -2.39 -1.26 -7.37 -4.68 -3.92 -0.61 -2.19 -11.28 -6.34 -3.59 -3.41
Bud Neck -5.35 -8.76 -10.01 -8.57 0.53 1.63 -9.57 -8.21 -9.09 7.40 -2.68 -11.09 -13.17 -12.32 3.57
Bud Site -4.12 -6.61 -8.15 -6.94 -6.03 -2.65 -3.92 -7.20 -6.37 -3.62 -4.79 -7.27 -10.90 -9.37 -7.76
Cell Periphery -5.17 -10.76 -8.50 -5.06 1.50 8.19 -5.69 -6.79 -11.04 0.02 2.18 -10.17 -11.09 -12.44 -0.95
Cytoplasm 10.53 22.99 24.92 15.47 2.07 -8.20 16.51 12.84 21.06 -10.10 1.72 22.90 27.97 26.81 -0.70
Cytoplasmic Foci -1.93 -9.37 -4.78 -2.75 6.20 1.60 -9.20 -0.33 -1.71 9.10 -0.94 -11.66 -4.24 -3.28 9.65
Eisosomes -2.84 -11.96 -4.22 -2.73 -1.77 -0.70 -9.03 -7.80 -6.68 4.87 -3.20 -13.89 -6.80 -5.24 -1.26
Endoplasmic Reticulum -1.90 -3.20 -3.03 -0.41 -0.94 0.50 2.01 -0.46 -0.91 -2.14 -2.02 -2.23 -3.10 -1.04 -0.85
Endosome -1.56 -6.19 -4.75 -4.53 -3.96 -1.64 -5.12 -2.69 -0.50 -0.90 -2.06 -6.54 -5.54 -3.14 -4.34
Golgi -1.76 -6.35 -0.45 0.92 4.29 7.32 2.03 8.99 3.13 3.88 3.46 -0.88 5.61 2.90 6.62
Lipid Particles -5.22 -10.55 -11.03 -6.43 -1.01 6.09 -4.79 -5.54 -10.70 1.99 1.55 -8.92 -11.56 -13.03 -1.88
Mitochondria -1.57 -7.77 -2.16 -1.86 -2.04 -2.65 -2.09 -2.14 -1.61 -1.97 -2.79 -6.95 -2.65 -2.26 -2.46
Mitotic Spindle 0.38 -5.27 -2.78 -2.81 -1.57 0.23 -0.49 0.94 1.16 1.74 -0.46 -1.94 -1.89 -1.61 -0.74
None -2.83 1.48 4.63 4.15 1.45 -1.03 5.48 5.50 5.14 0.40 -2.77 1.77 7.29 6.03 1.90
Nuclear Periphery -4.68 -8.81 -4.34 -1.34 6.45 4.50 -5.72 2.91 -2.38 6.26 0.20 -9.40 -2.23 -2.47 8.58
Nuclear Periphery Foci -4.07 -9.31 -2.40 1.38 5.70 5.42 -1.71 6.99 1.87 5.96 0.70 -5.69 3.03 2.25 7.98
Nucleolus -1.72 -12.32 -4.73 0.42 -3.60 -0.09 -2.31 -2.08 -2.18 -1.08 -1.86 -4.48 -4.99 -0.70 -3.84
Nucleus -6.41 -13.85 -6.78 0.39 8.02 2.41 -8.74 -2.73 -4.91 8.06 -3.13 -15.70 -7.15 -3.91 11.33
Peroxisomes -3.72 -5.46 -3.08 -2.90 -2.89 -1.17 -4.36 -4.82 0.04 -3.37 -1.32 -4.94 -4.05 -1.61 -3.71
Vacuole -10.21 -18.39 -24.39 -18.12 -7.87 6.46 -11.78 -18.12 -21.87 4.76 -3.69 -19.07 -30.89 -29.19 -6.43
Vacuole Periphery -0.97 -7.11 -3.47 -2.55 4.47 3.92 -0.12 4.95 1.35 2.81 1.56 -5.37 0.79 -0.85 6.00
Standard name
Human Ortholog
Description Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD3 has a paralog, IMD4, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Imd3

Imd3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Imd3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available