Standard name
Human Ortholog
Description Calcineurin A; one isoform (the other is Cmp2p) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1; regulates the function of Aly1p alpha-arrestin; CNA1 has a paralog, CMP2, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.06 0.05 0 0 0 0 0 0
Cytoplasm 1.0 0.98 1.0 0.99 0.96 0.96 0.89 – 0.87 0.83 0.76 0.78 1.0 0.99 0.97 0.95 0.93 0.95 0.99 0.97 0.92 0.89 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.06 0 0.18 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0.05 0.08 0.08 0.07 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 0 0 0 0 0 2 – 0 0 2 1 0 0 0 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 5 4 1 5 – 1 5 7 8 0 1 4 20 17 21 0 0 0 0 0 0
Cytoplasm 210 65 16 170 302 305 382 – 243 273 210 253 46 151 265 208 282 399 205 75 45 24 143 184
Endoplasmic Reticulum 1 1 0 2 4 6 12 – 5 9 5 8 0 0 1 4 5 15 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Golgi 0 0 0 0 0 1 0 – 0 1 0 0 0 0 2 1 2 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 1 3 7 – 16 14 51 45 0 1 1 2 1 1 0 0 0 0 0 0
Nucleus 3 0 0 0 0 5 16 – 12 19 11 20 0 0 4 2 12 15 1 1 0 0 2 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 – 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 5 9 14 – 14 25 23 23 0 0 2 7 14 13 0 0 1 0 1 0
Unique Cell Count 211 66 16 172 313 319 427 280 327 276 325 46 152 274 219 302 422 209 78 49 28 154 196
Labelled Cell Count 214 67 16 177 316 330 439 292 346 311 359 46 153 280 246 334 465 209 78 49 28 154 196


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 11.8 9.7 10.0 9.9 8.2 8.1 – 7.0 7.3 6.3 6.5 9.8 10.9 10.8 14.9 15.1 14.2 – 9.2 10.4
Std Deviation (1e-4) 1.1 1.9 1.2 1.3 1.4 1.2 1.3 – 1.2 1.2 1.3 1.2 1.5 1.5 1.6 3.2 2.6 3.1 – 1.5 1.5
Intensity Change (Log2) – – – 0.04 0.03 -0.25 -0.26 – -0.46 -0.42 -0.62 -0.57 0.02 0.16 0.15 0.62 0.63 0.54 – -0.09 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -0.8 -0.9 -1.4 – -1.6 -1.8 -2.2 -2.1 0 -0.3 -0.7 -0.9 -1.1 -1.0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0965 5.7324 4.9313 4.4804 4.8619 5.1046 4.5618 6.1365 5.3693 4.5018 4.6188 5.3832 3.0974 5.1236 4.4853 4.5636 3.5591 3.9888
Actin 0.0323 0.0032 0.0111 0.0003 0.0301 0.0023 0.018 0.0002 0.0215 0.0075 0.001 0.0141 0.0241 0.0006 0.0169 0.0562 0.023 0.0032
Bud 0.0028 0.0004 0.0003 0.0001 0.0033 0.0002 0.0008 0.0001 0.0005 0.0004 0.0004 0.0002 0.0006 0.0002 0.0006 0.0003 0.0008 0
Bud Neck 0.0036 0.0003 0.0008 0.0006 0.0018 0.0043 0.0005 0.0001 0.0005 0.0009 0.0004 0.0009 0.0041 0.0001 0.001 0.0049 0.0014 0.0008
Bud Periphery 0.0055 0.0002 0.0003 0 0.0058 0.0002 0.0009 0 0.0004 0.0004 0.0005 0.0001 0.0005 0 0.0006 0.0005 0.0009 0
Bud Site 0.0035 0.001 0.0011 0.0002 0.0125 0.0003 0.0049 0.0002 0.0027 0.0021 0.0012 0.0005 0.0108 0.0005 0.0026 0.0018 0.0016 0.0001
Cell Periphery 0.0002 0 0.0001 0 0.0006 0 0.0003 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0.0004 0.0002 0
Cytoplasm 0.7495 0.96 0.9244 0.8757 0.8421 0.9188 0.7845 0.9703 0.8965 0.9006 0.8058 0.9235 0.7761 0.9863 0.9559 0.8461 0.8428 0.9456
Cytoplasmic Foci 0.0355 0.0076 0.0085 0.0349 0.0115 0.0102 0.0172 0.0011 0.0169 0.0112 0.0094 0.0114 0.0505 0.0019 0.0031 0.0082 0.0139 0.0088
Eisosomes 0.0003 0 0.0001 0 0.001 0 0.0004 0 0.0002 0.0001 0 0 0.0002 0 0.0001 0.0005 0.0002 0
Endoplasmic Reticulum 0.008 0.0021 0.0024 0.0021 0.0034 0.0033 0.0266 0.0007 0.0019 0.0022 0.0018 0.0014 0.0046 0.0007 0.0016 0.0012 0.0012 0.0014
Endosome 0.0288 0.0027 0.0101 0.0052 0.0142 0.0143 0.0535 0.0002 0.0179 0.0102 0.0585 0.0195 0.0206 0.0003 0.0019 0.0148 0.0378 0.0127
Golgi 0.0093 0.0006 0.0036 0.001 0.0047 0.005 0.0136 0 0.0041 0.0152 0.0099 0.0067 0.0076 0.0001 0.0038 0.0017 0.0444 0.0054
Lipid Particles 0.0141 0 0.0029 0.0129 0.0024 0.0021 0.0148 0 0.0028 0.0027 0.003 0.0018 0.0136 0 0.0006 0.0011 0.005 0.0078
Mitochondria 0.0069 0.0002 0.0044 0.0003 0.0339 0.0078 0.0148 0 0.004 0.0252 0.0857 0.0023 0.002 0 0.0013 0.0017 0.0188 0.0002
None 0.0483 0.0132 0.0152 0.0487 0.0079 0.0129 0.0131 0.0252 0.011 0.0072 0.0025 0.0028 0.0526 0.007 0.0053 0.0075 0.0014 0.0016
Nuclear Periphery 0.0133 0.0011 0.0022 0.0015 0.0041 0.002 0.0171 0.0002 0.0013 0.0005 0.0017 0.0007 0.0033 0.0003 0.0006 0.0019 0.0005 0.0006
Nucleolus 0.0004 0 0.0001 0.0003 0.0015 0.0001 0.0004 0 0.0002 0.0001 0.0005 0 0.0009 0 0 0.0005 0.0001 0
Nucleus 0.0081 0.0044 0.0051 0.0095 0.0078 0.0117 0.0041 0.0012 0.0025 0.0018 0.0063 0.0028 0.004 0.0013 0.0018 0.0033 0.0014 0.0036
Peroxisomes 0.0119 0.0007 0.0029 0.0025 0.004 0.001 0.0058 0 0.0106 0.0103 0.0018 0.0093 0.0104 0.0001 0.0014 0.0006 0.0023 0.0072
Punctate Nuclear 0.0129 0.0014 0.003 0.003 0.004 0.0018 0.0028 0.0001 0.0005 0.0002 0.0008 0.0002 0.0112 0.0002 0.0002 0.0004 0.0002 0.0006
Vacuole 0.0032 0.0007 0.0009 0.0009 0.0025 0.0011 0.0035 0.0001 0.0027 0.0007 0.0066 0.0011 0.0015 0.0002 0.0004 0.0333 0.0014 0.0003
Vacuole Periphery 0.0014 0.0001 0.0003 0.0002 0.0009 0.0004 0.0023 0 0.0009 0.0003 0.002 0.0007 0.0004 0 0.0001 0.0132 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.9088 20.7721 18.4965 16.671 20.6587 10.6165 16.352 14.5804 19.5556 20.1189
Translational Efficiency 1.1657 1.0774 1.005 1.3964 1.2205 2.3885 1.0362 1.1674 0.932 1.223

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1351 296 66 273 1668 1404 209 1163 3019 1700 275 1436

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 885.97 950.07 1705.15 1363.37 918.06 1027.56 1436.42 1257.65 903.70 1014.07 1500.92 1277.75
Standard Deviation 107.70 262.90 301.26 231.59 127.67 151.74 155.52 178.69 120.21 178.65 230.95 194.36
Intensity Change Log 2 — 0.100776 0.944569 0.621847 — 0.162562 0.645817 0.454070 — 0.132549 0.800262 0.538905

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000135 0.000252 0.002088 0.002021 0.000216 0.000555 0.001600 0.001198 0.000179 0.000502 0.001717 0.001354
Bud Neck 0.000833 0.001176 0.004788 0.004135 0.000907 0.002475 0.003532 0.004659 0.000874 0.002249 0.003833 0.004559
Bud Site 0.003236 0.007912 0.008756 0.026702 0.003856 0.009181 0.029641 0.022970 0.003579 0.008960 0.024628 0.023680
Cell Periphery 0.000080 0.000184 0.000779 0.000215 0.000075 0.000101 0.000297 0.000155 0.000077 0.000116 0.000413 0.000166
Cytoplasm 0.850957* 0.748442* 0.513272* 0.646578* 0.808716* 0.754627* 0.566549* 0.590425* 0.827619* 0.753550* 0.553762* 0.601100*
Cytoplasmic Foci 0.044033 0.057433 0.024464 0.026930 0.073784 0.072456 0.024535 0.032154 0.060470 0.069840 0.024518 0.031161
Eisosomes 0.000010 0.000022 0.000024 0.000026 0.000014 0.000031 0.000025 0.000021 0.000012 0.000030 0.000025 0.000022
Endoplasmic Reticulum 0.000619 0.001020 0.003411 0.001111 0.001327 0.000774 0.002500 0.001485 0.001010 0.000817 0.002718 0.001414
Endosome 0.001499 0.004670 0.004097 0.022540 0.002096 0.006839 0.003662 0.018614 0.001829 0.006462 0.003766 0.019360
Golgi 0.000534 0.002255 0.001059 0.023411 0.001063 0.002568 0.000347 0.010419 0.000826 0.002514 0.000518 0.012889
Lipid Particles 0.000371 0.000874 0.004577 0.003258 0.001156 0.001461 0.002962 0.006051 0.000804 0.001359 0.003350 0.005520
Mitochondria 0.000761 0.000438 0.000354 0.020128 0.000288 0.001772 0.000325 0.009330 0.000500 0.001540 0.000332 0.011383
Mitotic Spindle 0.001736 0.004446 0.004180 0.023383 0.000447 0.002769 0.016293 0.079568 0.001024 0.003061 0.013386 0.068887
None 0.011722 0.019920 0.001691 0.005592 0.005029 0.002527 0.002967 0.006259 0.008024 0.005555 0.002661 0.006132
Nuclear Periphery 0.000143 0.000162 0.000902 0.000692 0.000114 0.000234 0.001146 0.001102 0.000127 0.000222 0.001087 0.001024
Nuclear Periphery Foci 0.001687 0.006017 0.015875 0.007665 0.002907 0.003027 0.008089 0.004586 0.002361 0.003548 0.009957 0.005171
Nucleolus 0.000240 0.001014 0.000184 0.000177 0.000196 0.000964 0.000124 0.000191 0.000216 0.000972 0.000139 0.000189
Nucleus 0.068424 0.068012 0.314267* 0.036765 0.065443 0.049831 0.126205 0.039755 0.066777 0.052997 0.171340* 0.039187
Peroxisomes 0.000348 0.000562 0.000182 0.001883 0.000387 0.001804 0.000333 0.001809 0.000370 0.001588 0.000296 0.001824
Vacuole 0.012014 0.074694 0.094688 0.143640 0.031734 0.085114 0.208567* 0.157193 0.022909 0.083299 0.181236 0.154616
Vacuole Periphery 0.000619 0.000497 0.000363 0.003151 0.000246 0.000888 0.000304 0.012055 0.000413 0.000820 0.000318 0.010362

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.97 -8.46 -3.22 -2.37 6.01 -1.77 -3.35 -3.13 0.35 2.10 -2.11 -6.60 -4.74 -0.13 4.59
Bud Neck -1.27 -8.12 -4.44 -4.02 5.09 -2.97 -8.05 -4.74 -0.82 2.22 -3.07 -10.56 -5.66 -1.52 4.34
Bud Site -5.35 -6.96 -5.57 -3.06 -0.33 -4.88 -3.40 -7.26 -2.27 0.36 -6.04 -4.63 -9.06 -3.13 0.32
Cell Periphery -3.07 -8.82 -4.80 -0.66 7.93 -1.98 -8.11 -2.12 0.40 7.24 -3.03 -9.86 -4.22 -0.09 9.03
Cytoplasm 8.56 15.22 18.18 8.83 -3.51 7.76 22.46 27.38 18.50 -7.63 12.74 27.72 34.72 20.53 -8.45
Cytoplasmic Foci -3.22 1.96 6.51 6.23 0.72 0.53 16.17 17.50 16.64 -1.19 -3.29 13.76 18.41 17.75 -0.34
Eisosomes -4.29 -6.32 -5.70 0.53 3.39 -2.82 -8.40 -2.55 2.39 7.39 -3.54 -11.05 -6.60 2.42 8.20
Endoplasmic Reticulum -1.69 -5.43 2.58 3.02 5.89 1.72 -1.42 2.49 1.85 4.64 1.15 -4.16 2.78 2.66 6.79
Endosome -3.00 -4.08 -1.48 0.41 0.98 -5.09 -3.40 -2.58 2.20 -0.93 -5.83 -5.49 -3.19 2.24 -0.31
Golgi -0.86 -5.51 -1.20 -0.33 -0.65 -1.87 2.94 -0.87 0.67 -2.02 -2.37 1.74 -1.54 0.46 -2.01
Lipid Particles -3.77 -6.58 -5.08 -4.13 3.34 -0.42 -5.64 -1.90 -1.56 1.37 -1.30 -9.38 -3.24 -2.22 2.66
Mitochondria 1.05 1.05 -0.66 -0.91 -0.87 -2.58 0.23 -1.49 0.08 -1.54 -1.98 1.25 -1.45 -0.26 -1.76
Mitotic Spindle -0.96 -1.81 -3.44 -1.85 -1.78 -2.15 -4.73 -6.81 -5.96 -1.85 -1.83 -4.81 -7.12 -6.23 -2.27
None -0.75 9.52 5.23 2.54 -3.27 5.23 1.97 3.33 -1.00 0.60 3.77 6.79 7.19 1.69 -0.42
Nuclear Periphery -0.47 -7.00 -4.59 -6.54 4.96 -1.37 -14.32 -6.35 -3.78 8.03 -1.23 -15.31 -6.69 -4.55 9.17
Nuclear Periphery Foci -4.62 -6.13 -4.24 1.95 5.32 -0.29 -5.07 0.31 0.59 5.29 -3.61 -7.82 -2.84 1.47 7.09
Nucleolus -2.34 0.37 0.69 2.62 0.61 -1.96 2.01 -0.21 1.88 -0.91 -2.22 1.61 0.12 2.21 -0.77
Nucleus 0.83 -8.94 5.19 1.98 9.72 5.59 -7.51 2.81 -2.64 8.23 5.89 -10.60 4.91 -1.25 11.58
Peroxisomes -1.35 1.03 -0.64 0.74 -1.79 -3.30 2.10 -1.87 2.45 -2.85 -3.43 1.97 -2.00 2.52 -3.15
Vacuole -9.89 -8.21 -19.46 -12.47 -5.41 -13.80 -17.59 -33.67 -23.90 3.24 -18.68 -18.80 -39.84 -26.96 0.98
Vacuole Periphery 1.08 1.57 0.97 0.10 -0.06 -2.83 1.71 -2.36 -1.51 -2.44 -1.79 2.41 -2.09 -1.49 -2.42
Standard name
Human Ortholog
Description Calcineurin A; one isoform (the other is Cmp2p) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1; regulates the function of Aly1p alpha-arrestin; CNA1 has a paralog, CMP2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cna1

Cna1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cna1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available