Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 33 | 10 | 9 | 0 | 3 | 2 | 1 | 6 | 7 | – | 4 | 1 | 0 | 0 | 0 | 18 | 21 | 21 | 17 | 10 | 15 | 4 | 3 | 6 |
Bud | 1 | 3 | 8 | 11 | 8 | 7 | 11 | 18 | 10 | – | 13 | 12 | 7 | 3 | 8 | 3 | 2 | 3 | 4 | 4 | 4 | 3 | 3 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 8 | 8 | 4 | 1 | 3 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 1 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 3 | 1 | 5 | 3 | 4 | 4 | 6 | 10 | – | 6 | 12 | 2 | 1 | 2 | 8 | 11 | 2 | 1 | 1 | 1 | 0 | 0 | 0 |
Cytoplasm | 177 | 244 | 211 | 206 | 254 | 266 | 322 | 342 | 224 | – | 87 | 172 | 116 | 204 | 235 | 373 | 344 | 361 | 159 | 220 | 149 | 116 | 143 | 123 |
Endoplasmic Reticulum | 25 | 32 | 18 | 2 | 5 | 19 | 6 | 5 | 21 | – | 10 | 4 | 0 | 2 | 6 | 27 | 18 | 23 | 23 | 15 | 18 | 5 | 11 | 19 |
Endosome | 53 | 11 | 22 | 14 | 35 | 14 | 22 | 30 | 2 | – | 0 | 4 | 6 | 4 | 1 | 37 | 38 | 26 | 120 | 51 | 45 | 8 | 8 | 6 |
Golgi | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 33 | 5 | 8 | 0 | 1 | 2 |
Mitochondria | 9 | 4 | 9 | 69 | 60 | 132 | 190 | 188 | 242 | – | 117 | 202 | 6 | 5 | 13 | 18 | 11 | 17 | 47 | 29 | 83 | 12 | 12 | 7 |
Nucleus | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | – | 4 | 2 | 0 | 0 | 0 | 32 | 24 | 14 | 2 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 9 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 2 | 0 | 0 | 0 |
SpindlePole | 12 | 1 | 5 | 1 | 3 | 0 | 4 | 8 | 0 | – | 2 | 0 | 0 | 0 | 0 | 7 | 8 | 5 | 15 | 5 | 3 | 1 | 2 | 1 |
Vac/Vac Membrane | 22 | 3 | 4 | 3 | 10 | 3 | 6 | 20 | 3 | – | 2 | 7 | 3 | 3 | 3 | 43 | 48 | 32 | 19 | 21 | 42 | 12 | 13 | 6 |
Unique Cell Count | 331 | 296 | 268 | 264 | 334 | 377 | 482 | 529 | 412 | 189 | 324 | 125 | 214 | 247 | 505 | 475 | 462 | 464 | 380 | 389 | 171 | 211 | 190 | |
Labelled Cell Count | 366 | 313 | 287 | 311 | 384 | 448 | 567 | 631 | 523 | 247 | 417 | 140 | 222 | 268 | 569 | 527 | 507 | 464 | 380 | 389 | 171 | 211 | 190 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 6.6 | 5.5 | 5.2 | 5.3 | 5.1 | 5.0 | 5.1 | 4.6 | – | 4.7 | 4.5 | 6.1 | 6.9 | 6.3 | 7.8 | 8.9 | 8.6 | 6.6 | 6.6 | 7.4 |
Std Deviation (1e-4) | 0.9 | 0.7 | 0.8 | 1.4 | 1.4 | 1.1 | 1.3 | 1.3 | 1.2 | – | 1.4 | 1.4 | 2.0 | 2.3 | 1.9 | 1.4 | 1.5 | 1.6 | 1.8 | 1.5 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.05 | -0.11 | -0.14 | -0.11 | -0.27 | – | -0.21 | -0.28 | 0.16 | 0.33 | 0.19 | 0.51 | 0.7 | 0.65 | 0.27 | 0.26 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -2.1 | 0 | 0 | -2.2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0.1 | 0.7 | 0.8 |
Bud | 0.7 | -0.4 | -0.9 | -0.6 | 0.3 | 0 | – | 0 | 0 | 0 | 0 | 0.2 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.2 | -0.8 | -2.3 | -3.5 | -4.1 | -6.5 | – | -7.2 | -6.5 | 3.5 | 5.2 | 5.5 | -1.5 | -1.9 | -0.2 |
Endoplasmic Reticulum | -3.6 | -3.3 | -0.9 | -4.1 | -4.6 | 0 | – | 0 | 0 | -3.0 | -3.2 | -2.3 | -0.8 | -1.8 | -1.0 |
Endosome | -1.3 | 0.9 | -2.5 | -2.0 | -1.4 | 0 | – | 0 | 0 | -1.2 | -3.1 | -4.3 | -0.4 | -0.1 | -1.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 7.4 | 5.6 | 9.6 | 10.7 | 9.9 | 0 | – | 0 | 0 | 0 | -0.7 | 1.1 | 0.1 | -0.8 | 0.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.2 | 3.7 | 2.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.2 | 0 | 0 | 1.8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.9 | 4.4 | 3.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.0768 | 6.6648 | 6.1776 | 5.8582 | 5.3169 | 6.1252 | 3.7331 | 5.0926 | 4.9803 | 4.5454 | 4.4567 | 4.5194 | 4.5282 | 4.9382 | 4.1024 | 5.1076 | 5.4977 | 4.7192 |
Actin | 0.0069 | 0.0079 | 0.0147 | 0.0046 | 0.0041 | 0.0054 | 0.0353 | 0.0016 | 0.0165 | 0.0081 | 0.0098 | 0.0117 | 0.0034 | 0.0063 | 0.0109 | 0.0223 | 0.0204 | 0.0025 |
Bud | 0.0005 | 0.0005 | 0.0014 | 0.0006 | 0.0004 | 0.0004 | 0.0009 | 0.0007 | 0.0027 | 0.0003 | 0.0007 | 0.0008 | 0.0001 | 0.0002 | 0.0031 | 0.001 | 0.0003 | 0.0002 |
Bud Neck | 0.0057 | 0.0024 | 0.0016 | 0.0011 | 0.0303 | 0.0114 | 0.0024 | 0.0005 | 0.0027 | 0.0005 | 0.0021 | 0.0034 | 0.0014 | 0.0003 | 0.0011 | 0.0012 | 0.0225 | 0.0016 |
Bud Periphery | 0.0005 | 0.0003 | 0.0011 | 0.0007 | 0.0002 | 0.001 | 0.0008 | 0.0004 | 0.0024 | 0.0001 | 0.0008 | 0.0008 | 0 | 0.0001 | 0.0021 | 0.0009 | 0.0002 | 0.0001 |
Bud Site | 0.0034 | 0.0161 | 0.0046 | 0.0013 | 0.0009 | 0.0009 | 0.0035 | 0.0123 | 0.0096 | 0.0007 | 0.0014 | 0.0008 | 0.0014 | 0.0016 | 0.0052 | 0.0023 | 0.0008 | 0.0004 |
Cell Periphery | 0.0019 | 0.0019 | 0.0014 | 0.0005 | 0.0005 | 0.0022 | 0.0004 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0002 |
Cytoplasm | 0.5033 | 0.6285 | 0.5307 | 0.5558 | 0.5035 | 0.6338 | 0.3772 | 0.6605 | 0.5679 | 0.594 | 0.5035 | 0.6732 | 0.4329 | 0.5501 | 0.5045 | 0.3543 | 0.4918 | 0.6257 |
Cytoplasmic Foci | 0.1251 | 0.1267 | 0.1521 | 0.1094 | 0.1893 | 0.1217 | 0.1113 | 0.1077 | 0.1056 | 0.1529 | 0.116 | 0.1209 | 0.1522 | 0.1565 | 0.1683 | 0.1186 | 0.1463 | 0.1449 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0421 | 0.0209 | 0.0173 | 0.0107 | 0.0063 | 0.0212 | 0.0171 | 0.0089 | 0.012 | 0.0069 | 0.0242 | 0.0149 | 0.0157 | 0.0094 | 0.0151 | 0.0183 | 0.0054 | 0.0163 |
Endosome | 0.1817 | 0.1138 | 0.1789 | 0.2344 | 0.1946 | 0.1402 | 0.2025 | 0.1102 | 0.1351 | 0.1888 | 0.2086 | 0.1019 | 0.1255 | 0.1045 | 0.1862 | 0.2076 | 0.072 | 0.1205 |
Golgi | 0.0222 | 0.0291 | 0.0388 | 0.0319 | 0.0368 | 0.0313 | 0.0453 | 0.0093 | 0.019 | 0.0225 | 0.0447 | 0.0158 | 0.047 | 0.0139 | 0.0277 | 0.0501 | 0.0316 | 0.0254 |
Lipid Particles | 0.0155 | 0.0042 | 0.0063 | 0.0026 | 0.0039 | 0.0027 | 0.0355 | 0.0027 | 0.007 | 0.0011 | 0.0045 | 0.0065 | 0.0125 | 0.002 | 0.0072 | 0.0025 | 0.0019 | 0.0019 |
Mitochondria | 0.0025 | 0.0017 | 0.005 | 0.0023 | 0.0018 | 0.0059 | 0.0132 | 0.0062 | 0.0171 | 0.0019 | 0.0528 | 0.0029 | 0.0011 | 0.0005 | 0.0058 | 0.0099 | 0.0011 | 0.0017 |
None | 0.0662 | 0.0315 | 0.0297 | 0.0037 | 0.0101 | 0.0068 | 0.0884 | 0.0689 | 0.0785 | 0.0131 | 0.0057 | 0.0319 | 0.201 | 0.1338 | 0.0452 | 0.2 | 0.1917 | 0.049 |
Nuclear Periphery | 0.0023 | 0.001 | 0.001 | 0.0017 | 0.0008 | 0.0008 | 0.0128 | 0.0008 | 0.0024 | 0.001 | 0.0014 | 0.0012 | 0.0008 | 0.0005 | 0.0018 | 0.0012 | 0.0009 | 0.0006 |
Nucleolus | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0009 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0006 | 0 | 0.0002 | 0.0001 |
Nucleus | 0.0011 | 0.001 | 0.0008 | 0.0015 | 0.0007 | 0.0009 | 0.0024 | 0.0009 | 0.0012 | 0.0006 | 0.0007 | 0.0023 | 0.0002 | 0.0004 | 0.0009 | 0.0004 | 0.0006 | 0.0005 |
Peroxisomes | 0.0041 | 0.0045 | 0.0052 | 0.009 | 0.0045 | 0.0064 | 0.0147 | 0.0021 | 0.009 | 0.0018 | 0.0104 | 0.0038 | 0.0032 | 0.0151 | 0.0062 | 0.0036 | 0.0078 | 0.0022 |
Punctate Nuclear | 0.0012 | 0.0006 | 0.0011 | 0.0005 | 0.0006 | 0.0004 | 0.0094 | 0.0008 | 0.0023 | 0.0005 | 0.0003 | 0.0024 | 0.0006 | 0.0005 | 0.0018 | 0.0005 | 0.0013 | 0.0016 |
Vacuole | 0.0122 | 0.0062 | 0.0066 | 0.0247 | 0.0086 | 0.0048 | 0.0205 | 0.0042 | 0.0062 | 0.0039 | 0.0096 | 0.0031 | 0.0005 | 0.0032 | 0.0045 | 0.0036 | 0.0024 | 0.0037 |
Vacuole Periphery | 0.0013 | 0.0009 | 0.0016 | 0.0029 | 0.002 | 0.0014 | 0.0053 | 0.0007 | 0.0017 | 0.0014 | 0.0025 | 0.0011 | 0.0003 | 0.0006 | 0.0013 | 0.0013 | 0.0005 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.6257 | 14.1832 | 8.8023 | 19.6189 | 15.3347 | 15.5689 | 24.4442 | 23.5814 | 16.9942 | 26.2102 |
Translational Efficiency | 0.3057 | 0.4369 | 0.5017 | 0.2226 | 0.3546 | 0.4187 | 0.2965 | 0.2688 | 0.3331 | 0.2302 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1874 | 924 | 618 | 1428 | 1854 | 2305 | 122 | 1372 | 3728 | 3229 | 740 | 2800 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 783.62 | 821.54 | 1140.40 | 973.54 | 731.54 | 831.27 | 1069.56 | 962.75 | 757.72 | 828.49 | 1128.72 | 968.25 |
Standard Deviation | 92.38 | 128.21 | 141.23 | 119.66 | 110.05 | 105.40 | 113.18 | 119.81 | 104.84 | 112.49 | 139.50 | 119.85 |
Intensity Change Log 2 | — | 0.068177 | 0.541314 | 0.313086 | — | 0.184380 | 0.548009 | 0.396224 | — | 0.125451 | 0.544550 | 0.353825 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001322 | 0.001632 | 0.013596 | 0.004225 | 0.001147 | 0.001424 | 0.007832 | 0.010751 | 0.001235 | 0.001483 | 0.012646 | 0.007423 |
Bud Neck | 0.012059 | 0.009218 | 0.004156 | 0.007929 | 0.011427 | 0.012176 | 0.006475 | 0.006241 | 0.011744 | 0.011330 | 0.004539 | 0.007102 |
Bud Site | 0.019748 | 0.019688 | 0.059133 | 0.048779 | 0.016711 | 0.026922 | 0.033925 | 0.045955 | 0.018238 | 0.024852 | 0.054977 | 0.047395 |
Cell Periphery | 0.000132 | 0.000123 | 0.000225 | 0.000070 | 0.000098 | 0.000079 | 0.000148 | 0.000097 | 0.000115 | 0.000092 | 0.000212 | 0.000083 |
Cytoplasm | 0.122973* | 0.141087* | 0.180593* | 0.268192* | 0.170753* | 0.199106* | 0.085646 | 0.203630* | 0.146734* | 0.182503* | 0.164940* | 0.236556* |
Cytoplasmic Foci | 0.473645* | 0.481443* | 0.351015* | 0.250210* | 0.484264* | 0.448017* | 0.299559* | 0.344821* | 0.478926* | 0.457582* | 0.342532* | 0.296570* |
Eisosomes | 0.000263 | 0.000140 | 0.000059 | 0.000046 | 0.000298 | 0.000180 | 0.000122 | 0.000099 | 0.000280 | 0.000168 | 0.000069 | 0.000072 |
Endoplasmic Reticulum | 0.001368 | 0.001398 | 0.003919 | 0.000508 | 0.001799 | 0.000798 | 0.001911 | 0.000738 | 0.001582 | 0.000969 | 0.003588 | 0.000621 |
Endosome | 0.101581 | 0.146464 | 0.094383 | 0.096710 | 0.102600 | 0.093483 | 0.210697* | 0.101049 | 0.102088 | 0.108644 | 0.113559 | 0.098836 |
Golgi | 0.179600* | 0.088073 | 0.050302 | 0.010596 | 0.099067 | 0.112697* | 0.011713 | 0.016436 | 0.139549* | 0.105651 | 0.043940 | 0.013458 |
Lipid Particles | 0.012275 | 0.011639 | 0.015869 | 0.004206 | 0.019249 | 0.005730 | 0.025393 | 0.008280 | 0.015743 | 0.007421 | 0.017439 | 0.006202 |
Mitochondria | 0.019685 | 0.009904 | 0.000186 | 0.002149 | 0.016124 | 0.014502 | 0.000165 | 0.001552 | 0.017914 | 0.013186 | 0.000183 | 0.001856 |
Mitotic Spindle | 0.005996 | 0.009362 | 0.121836* | 0.052454 | 0.004194 | 0.010982 | 0.025276 | 0.025992 | 0.005100 | 0.010519 | 0.105917* | 0.039488 |
None | 0.000373 | 0.000636 | 0.000169 | 0.000272 | 0.001405 | 0.000252 | 0.000060 | 0.000123 | 0.000886 | 0.000362 | 0.000151 | 0.000199 |
Nuclear Periphery | 0.000061 | 0.000147 | 0.000312 | 0.000291 | 0.000289 | 0.000082 | 0.000346 | 0.000129 | 0.000174 | 0.000100 | 0.000318 | 0.000211 |
Nuclear Periphery Foci | 0.000300 | 0.000794 | 0.009907 | 0.001126 | 0.000977 | 0.000187 | 0.009271 | 0.001174 | 0.000636 | 0.000361 | 0.009802 | 0.001149 |
Nucleolus | 0.000299 | 0.000638 | 0.000093 | 0.000335 | 0.001479 | 0.000335 | 0.000524 | 0.000127 | 0.000886 | 0.000421 | 0.000164 | 0.000233 |
Nucleus | 0.003177 | 0.003759 | 0.004554 | 0.005786 | 0.007836 | 0.002930 | 0.004512 | 0.003820 | 0.005494 | 0.003167 | 0.004547 | 0.004823 |
Peroxisomes | 0.020070 | 0.017391 | 0.009831 | 0.042455 | 0.014138 | 0.018990 | 0.053149 | 0.067113 | 0.017120 | 0.018533 | 0.016973 | 0.054537 |
Vacuole | 0.018616 | 0.047900 | 0.077156 | 0.201619* | 0.039777 | 0.045172 | 0.217675* | 0.160651 | 0.029140 | 0.045953 | 0.100323 | 0.181545 |
Vacuole Periphery | 0.006458 | 0.008563 | 0.002705 | 0.002044 | 0.006368 | 0.005957 | 0.005602 | 0.001223 | 0.006413 | 0.006703 | 0.003183 | 0.001642 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.74 | -5.41 | -3.23 | -1.99 | 4.64 | -0.75 | -2.18 | -6.21 | -6.14 | -0.40 | -1.02 | -5.81 | -6.93 | -6.57 | 3.26 |
Bud Neck | 2.42 | 7.92 | 4.37 | 1.74 | -6.16 | -0.81 | 3.93 | 7.45 | 9.35 | 0.84 | 0.62 | 10.94 | 7.85 | 8.31 | -5.12 |
Bud Site | 0.05 | -10.71 | -6.58 | -6.25 | 5.57 | -5.67 | -2.57 | -9.52 | -5.19 | -1.15 | -4.86 | -11.41 | -11.38 | -7.06 | 4.66 |
Cell Periphery | 0.26 | -3.84 | 5.02 | 2.68 | 8.45 | 1.78 | -2.73 | 0.91 | -1.24 | 3.55 | 1.96 | -5.23 | 4.65 | 2.21 | 8.32 |
Cytoplasm | -2.41 | -6.07 | -19.43 | -15.33 | -8.19 | -4.10 | 5.21 | -6.23 | -2.85 | -8.46 | -6.99 | -2.91 | -18.27 | -11.67 | -8.32 |
Cytoplasmic Foci | -0.89 | 14.12 | 39.97 | 32.94 | 13.83 | 5.44 | 11.67 | 23.36 | 19.15 | -1.51 | 4.24 | 18.05 | 43.58 | 37.37 | 8.74 |
Eisosomes | 4.73 | 11.36 | 13.05 | 5.14 | 3.33 | 1.91 | 2.90 | 3.54 | 5.70 | 2.92 | 3.37 | 6.79 | 7.09 | 8.34 | 1.23 |
Endoplasmic Reticulum | -0.08 | -5.38 | 3.57 | 3.65 | 8.30 | 3.53 | -0.85 | 5.11 | 0.61 | 4.56 | 2.75 | -5.38 | 6.05 | 2.49 | 8.68 |
Endosome | -7.35 | 2.17 | 8.88 | 13.61 | 4.85 | 2.42 | -4.51 | 4.42 | 2.65 | 5.56 | -2.06 | -0.68 | 9.38 | 11.09 | 5.99 |
Golgi | 15.41 | 24.00 | 38.80* | 19.79 | 11.31 | -3.16 | 19.25 | 26.90 | 30.86 | -0.69 | 9.13 | 24.53 | 45.91* | 37.19 | 10.23 |
Lipid Particles | 0.41 | -3.29 | 8.98 | 6.45 | 10.57 | 9.99 | -0.88 | 8.06 | -3.12 | 2.62 | 9.33 | -1.58 | 11.62 | 2.58 | 8.20 |
Mitochondria | 5.47 | 15.68 | 14.20 | 6.43 | -3.00 | 1.11 | 13.70 | 12.10 | 13.92 | -2.63 | 4.25 | 20.80 | 18.63 | 15.50 | -3.96 |
Mitotic Spindle | -1.87 | -13.57 | -10.64 | -9.29 | 7.71 | -5.56 | -2.83 | -7.76 | -5.03 | 0.64 | -5.21 | -13.79 | -13.12 | -10.44 | 8.88 |
None | -1.22 | 1.93 | 0.17 | 1.34 | -2.27 | 3.54 | 3.96 | 3.89 | 2.43 | -0.39 | 2.89 | 4.15 | 3.76 | 1.64 | -1.39 |
Nuclear Periphery | -2.82 | -6.36 | -14.38 | -9.43 | -2.06 | 2.85 | -2.30 | 1.14 | -6.01 | 3.28 | 1.92 | -4.31 | -4.73 | -12.26 | 1.40 |
Nuclear Periphery Foci | -3.12 | -6.91 | -8.38 | -3.94 | 6.18 | 3.66 | -2.89 | -2.24 | -8.74 | 2.79 | 2.15 | -7.23 | -6.15 | -10.62 | 6.70 |
Nucleolus | -2.62 | 2.49 | -1.98 | 1.60 | -9.01 | 4.19 | 3.11 | 5.07 | 1.65 | 3.67 | 3.08 | 5.08 | 4.25 | 1.32 | -3.93 |
Nucleus | -0.74 | -2.57 | -8.12 | -7.71 | -4.66 | 5.25 | 1.23 | 2.28 | -4.33 | 0.44 | 3.89 | -0.24 | -3.55 | -9.68 | -2.84 |
Peroxisomes | 1.58 | 6.90 | -4.07 | -5.03 | -8.78 | -3.85 | -4.03 | -13.15 | -11.33 | -0.66 | -1.36 | 0.68 | -12.81 | -11.78 | -10.32 |
Vacuole | -9.22 | -12.94 | -39.16 | -32.67 | -23.14 | -2.25 | -9.64 | -27.83 | -26.85 | 0.88 | -9.17 | -14.08 | -47.20 | -42.59 | -18.70 |
Vacuole Periphery | -2.65 | 7.66 | 10.67 | 10.54 | 3.66 | 0.85 | 0.53 | 13.85 | 14.74 | 1.15 | -0.71 | 5.51 | 16.91 | 17.53 | 3.03 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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