Standard name
Human Ortholog
Description L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress; human homolog OAT complements yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.93 0.88 0.82 0.73 0.67 0.66 0.66 0.69 0.58 0.98 0.98 0.95 0.99 0.98 0.99 0.83 0.92 0.94 0.94 0.92 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.09 0.06 0.11 0.08 0.12 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0.1 0.13 0.18 0.27 0.22 0.21 0.22 0.27 0 0 0.06 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 2 4 3 3 2 0 0 0 0 0 0 1 0 1 1 1 0
Bud 0 1 0 0 0 1 9 7 1 3 5 6 0 1 1 0 0 0 19 9 10 8 10 14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 1 3 9 7 14 13 21 22 15 22 3 3 2 1 0 0 0 0 1 0 0 0
Cytoplasm 289 272 471 325 412 441 435 412 369 514 323 350 290 409 405 145 211 175 245 249 440 285 377 366
Endoplasmic Reticulum 1 2 2 4 5 8 19 13 14 47 5 23 1 3 4 6 8 12 9 0 6 0 4 2
Endosome 0 0 0 0 1 1 1 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0
Golgi 0 0 0 1 0 0 0 3 2 2 0 0 0 0 0 0 1 0 1 0 0 0 0 0
Mitochondria 0 0 0 2 5 25 55 38 59 59 56 78 2 1 0 0 0 0 0 0 1 0 0 0
Nucleus 0 1 1 1 2 1 2 6 9 15 4 6 0 0 0 0 0 1 2 5 0 3 5 4
Nuclear Periphery 0 0 0 0 0 0 1 1 0 2 1 1 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 0 0 0 0 2 1 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 3
Vac/Vac Membrane 8 2 9 23 47 68 109 168 124 163 105 160 3 10 27 2 3 2 5 2 0 1 7 4
Unique Cell Count 297 275 482 349 466 535 597 616 557 779 471 603 296 419 428 147 215 177 295 272 469 303 412 402
Labelled Cell Count 301 279 484 359 481 555 647 664 605 831 517 648 300 427 440 154 223 190 295 272 469 303 412 402


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.8 22.9 17.6 15.5 15.2 13.5 13.4 13.7 13.5 13.6 12.7 12.7 19.0 23.2 26.5 62.1 59.6 60.2 20.1 18.1 17.7
Std Deviation (1e-4) 6.4 8.2 5.9 5.6 4.9 4.3 4.3 4.2 4.5 4.6 4.7 4.8 7.5 10.1 14.0 23.4 23.2 20.6 6.6 5.8 5.6
Intensity Change (Log2) – – – -0.18 -0.21 -0.38 -0.4 -0.36 -0.38 -0.37 -0.47 -0.47 0.11 0.4 0.59 1.82 1.76 1.78 0.19 0.04 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.0 2.8 4.0 3.4 3.6 3.9 0 0 0 0 0 0
Cytoplasm -3.3 -5.7 -8.0 -11.1 -12.8 -12.9 -13.2 -12.1 -15.1 0.2 -0.1 -2.5 0.7 0.4 0.9
Endoplasmic Reticulum 0 0 0 3.3 2.4 2.7 5.0 0 3.7 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.8 6.8 5.5 7.4 6.2 7.8 8.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 2.6 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.5 5.4 6.5 8.6 11.4 9.8 9.6 9.7 11.1 0 0.5 3.4 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.2929 11.1568 9.9819 10.8289 10.6766 10.0648 6.3503 8.6162 7.6494 7.8549 9.1247 7.3146 5.9131 8.2588 7.4559 5.4437 4.8131 7.737
Actin 0.0282 0.0007 0.0127 0.0136 0.0005 0.003 0.006 0.0007 0.012 0.0015 0.0001 0.0001 0.0248 0.0005 0.0089 0.03 0.0005 0.0027
Bud 0.0005 0.0013 0.0012 0.0014 0.0001 0.0006 0.0022 0.001 0.0043 0.0005 0.0001 0.0001 0.0009 0.0004 0.0011 0.002 0.0005 0.0002
Bud Neck 0.0008 0.0003 0.0005 0.0005 0.0005 0.0024 0.001 0.0001 0.0007 0.0004 0.0003 0.0007 0.0037 0.0002 0.0004 0.0007 0.0008 0.0008
Bud Periphery 0.0004 0.0005 0.0008 0.0016 0.0001 0.0004 0.0025 0.0002 0.0037 0.0006 0.0001 0.0001 0.0008 0.0001 0.0004 0.0011 0.0004 0.0002
Bud Site 0.002 0.0045 0.0038 0.0129 0.0001 0.0002 0.0032 0.0007 0.0087 0.0002 0 0.0004 0.0025 0.0009 0.0009 0.0011 0.0002 0.0001
Cell Periphery 0.0002 0.0001 0.0002 0.0004 0 0.0001 0.0002 0 0.0005 0 0 0.0001 0.0002 0 0 0.0001 0.0001 0
Cytoplasm 0.8498 0.9764 0.9055 0.8916 0.8824 0.9466 0.8404 0.9874 0.9152 0.862 0.8439 0.957 0.8371 0.975 0.9467 0.8323 0.9265 0.943
Cytoplasmic Foci 0.023 0.0024 0.0097 0.008 0.007 0.0085 0.0187 0.0013 0.0078 0.0039 0.0014 0.0019 0.0171 0.0016 0.0077 0.0079 0.0042 0.0053
Eisosomes 0.0012 0 0.0009 0.0002 0 0.0001 0 0 0 0 0 0 0.0002 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0118 0.0008 0.0031 0.0112 0.0055 0.0033 0.003 0.0005 0.0011 0.0009 0.0044 0.0011 0.005 0.0008 0.0033 0.0018 0.0032 0.0016
Endosome 0.0262 0.0008 0.0169 0.0161 0.0298 0.0037 0.0151 0.0002 0.021 0.0193 0.0911 0.0048 0.0286 0.0004 0.009 0.0106 0.0267 0.0045
Golgi 0.0032 0.0001 0.0074 0.0059 0.0009 0.0009 0.0026 0 0.0068 0.0384 0.0056 0.0002 0.0058 0 0.0011 0.0087 0.0017 0.002
Lipid Particles 0.0029 0 0.005 0.0019 0.0022 0.0002 0.0115 0 0.0006 0.0002 0.0001 0.0003 0.0017 0 0.0005 0.0028 0.0001 0.0023
Mitochondria 0.0017 0.0002 0.0035 0.0134 0.0004 0.0009 0.0056 0.0001 0.0027 0.0612 0.0073 0.0002 0.0136 0.0001 0.0003 0.0713 0.0067 0.0022
None 0.0101 0.0028 0.0108 0.004 0.0452 0.0119 0.0069 0.003 0.0034 0.0022 0.0006 0.0018 0.0116 0.0119 0.0028 0.0095 0.0076 0.0039
Nuclear Periphery 0.0087 0.0008 0.0025 0.0028 0.0073 0.0015 0.0308 0.0005 0.0011 0.001 0.005 0.002 0.0158 0.0008 0.0038 0.0019 0.002 0.0057
Nucleolus 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.0008 0 0.0002 0 0 0.0013 0.0004 0 0.0001 0.0001 0.0001 0
Nucleus 0.0098 0.0066 0.0065 0.0098 0.0137 0.0108 0.0322 0.0035 0.005 0.0051 0.0111 0.0125 0.0207 0.0062 0.0099 0.0098 0.0092 0.0192
Peroxisomes 0.0061 0.0002 0.004 0.002 0.0002 0.0029 0.0051 0 0.0005 0.0001 0 0 0.0013 0 0.0007 0.0044 0.0001 0.0041
Punctate Nuclear 0.0097 0.0008 0.0035 0.0007 0.0017 0.0006 0.0045 0.0003 0.0028 0.0002 0.0003 0.0005 0.0022 0.0004 0.0011 0.0014 0.0006 0.0011
Vacuole 0.0029 0.0006 0.0009 0.0013 0.0019 0.0009 0.0037 0.0003 0.0015 0.0008 0.0102 0.0134 0.0043 0.0006 0.0009 0.0016 0.0065 0.0007
Vacuole Periphery 0.0008 0.0001 0.0003 0.0005 0.0006 0.0003 0.0039 0 0.0004 0.0016 0.0181 0.0013 0.0016 0.0001 0.0003 0.0009 0.0024 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.77 51.8331 88.4061 86.1469 55.5414 31.1377 56.1239 65.2009 75.2338 40.3046
Translational Efficiency 1.1078 1.3161 1.3861 1.8104 1.4744 0.9559 1.4431 1.6128 1.2637 1.2107

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress; human homolog OAT complements yeast null mutant
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Car2

Car2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Car2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available