Standard name
Human Ortholog
Description Component of the NuA3b histone acetyltransferase complex; regulates interaction between NuA3b and H3K36me3 at the transcribed regions of genes; contains PWWP domain; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.12 0.06 0.06 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0.09 0 0 0 0.2 0.1 0.1 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.94 0.94 0.94 0.86 0.87 0.83 0.83 0.72 0.75 0.77 0.74 0.9 0.86 0.81 0.88 0.92 0.89 0.9 0.93 0.88 0.65 0.64 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.06 0 0.09 0.1 0.16 0.26 0.23 0.45 0.38 0.41 0.35 0.11 0.09 0.12 0 0 0 0 0 0.05 0.16 0.17 0.25
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2 0 0 1 1 2 2 4 0 0 0 1 0 0 9 2 0 2 1 2
Bud Neck 0 0 0 0 1 1 2 2 3 4 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 3 2 3 5 16 10 12 20 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 8 10 13 16 12 10 3 6 3 4 9 20 29 9 7 8 0 1 0 5 7 6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 3 0 0 0 0 0 2
Endosome 1 2 0 0 1 0 1 1 0 0 0 1 0 0 0 1 0 0 0 2 2 2 1 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 1
Mitochondria 6 24 2 1 28 13 21 13 114 52 59 102 1 0 1 4 2 2 1 5 0 2 2 5
Nucleus 402 481 282 250 279 452 456 471 400 386 446 473 151 177 194 133 116 109 392 501 283 101 166 116
Nuclear Periphery 1 6 0 0 1 6 4 4 4 9 8 2 0 1 1 1 0 0 0 1 1 1 2 4
Nucleolus 48 32 13 23 34 84 140 128 251 195 241 222 18 18 29 6 3 4 14 8 16 25 43 61
Peroxisomes 1 0 0 0 0 0 0 1 0 1 1 0 0 0 0 2 0 1 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 1 4 6 0 5 2 3 1 3 5 0 0 0 4 1 1 2 5 3
Vac/Vac Membrane 4 2 1 1 4 1 2 8 1 2 3 2 1 0 2 2 2 3 9 8 10 5 22 29
Unique Cell Count 424 512 299 267 324 522 548 567 558 517 582 639 167 205 240 151 126 123 435 537 320 156 261 245
Labelled Cell Count 467 551 307 286 366 576 645 650 793 672 779 835 181 219 261 161 130 130 435 537 320 156 261 245


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 7.6 9.8 8.9 7.2 7.8 7.4 7.8 5.9 6.5 6.4 6.3 9.3 8.9 8.5 8.7 11.1 11.2 9.2 10.0 10.3
Std Deviation (1e-4) 2.6 1.6 1.8 1.9 1.9 1.7 1.7 1.6 1.3 1.5 1.4 1.5 2.8 2.8 3.5 2.2 2.5 2.8 2.2 2.7 2.3
Intensity Change (Log2) – – – -0.12 -0.44 -0.32 -0.4 -0.33 -0.72 -0.58 -0.61 -0.64 -0.08 -0.13 -0.19 -0.17 0.18 0.19 -0.09 0.03 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.0 0 0 3.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.9 0.3 -0.4 -0.9 0 -1.6 0 0 1.5 3.4 4.3 1.7 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 4.6 1.9 2.7 1.7 8.1 5.2 5.2 7.0 0 0 0 0 0 0
Nucleus -0.3 -3.4 -3.5 -4.6 -4.7 -7.8 -7.0 -6.6 -7.3 -1.6 -3.1 -4.8 -2.3 -0.9 -2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.1 2.9 5.0 7.7 6.9 12.3 10.5 11.5 10.0 2.7 2.0 3.3 -0.2 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.0346 6.4923 5.9946 6.2287 4.7802 5.6937 10.0014 9.5966 9.487 9.3639 8.4374 9.194 7.3939 6.8978 6.663 6.6673 6.4208 6.4903
Actin 0.0319 0 0.0196 0 0.0117 0.0034 0.013 0 0.0031 0 0 0.001 0.015 0 0.0078 0.0003 0 0.0007
Bud 0.0005 0 0.0003 0 0.0004 0 0.0009 0 0.0001 0 0.0002 0.0001 0.0007 0 0.0002 0.0002 0.0001 0
Bud Neck 0.0006 0 0.0003 0 0.0006 0.0006 0.0014 0 0.0001 0 0.0004 0.0008 0.0022 0 0.0001 0.0002 0.0004 0.0006
Bud Periphery 0.0008 0 0.0003 0 0.0004 0.0001 0.0033 0 0.0001 0 0.0004 0.0003 0.0025 0 0.0005 0.0011 0.0002 0.0001
Bud Site 0.0011 0 0.0103 0 0.0048 0.0001 0.005 0 0.0004 0 0.0002 0.0002 0.0035 0 0.0008 0.0004 0.0001 0
Cell Periphery 0.0002 0 0.0001 0 0.0001 0 0.0011 0 0.0001 0 0.0002 0.0001 0.0006 0 0.0002 0.0006 0.0001 0
Cytoplasm 0.0016 0 0.0024 0 0.0003 0 0.0033 0 0.0019 0.0037 0 0.0001 0.0048 0 0.0019 0 0.0006 0.0001
Cytoplasmic Foci 0.0197 0 0.007 0 0.0059 0.0006 0.0193 0 0.0051 0.0001 0 0.0003 0.0101 0 0.0023 0.0001 0 0.0006
Eisosomes 0.0002 0 0.0001 0 0.0001 0.0004 0.0001 0 0.0001 0 0 0 0.0004 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.003 0 0.0002 0 0.0003 0.0005 0.0022 0 0.0026 0 0 0.0009 0.003 0 0.001 0.0001 0.0002 0
Endosome 0.0155 0 0.0023 0 0.0083 0.0017 0.0056 0 0.0056 0 0 0.0011 0.0068 0 0.0045 0.0006 0.0003 0.0002
Golgi 0.0143 0 0.0025 0 0.0073 0.0032 0.0042 0 0.0035 0 0 0.0003 0.0022 0 0.0011 0.0002 0 0.0003
Lipid Particles 0.0276 0 0.0028 0 0.0075 0.0113 0.0023 0 0.0036 0 0 0.0007 0.0055 0 0.0091 0.001 0.0001 0.0038
Mitochondria 0.0066 0.0001 0.0014 0 0.0043 0.0039 0.0103 0.0001 0.0026 0 0.0028 0.0048 0.005 0.0001 0.0068 0.0223 0.0007 0.0007
None 0.0032 0 0.0004 0 0.0002 0.0009 0.0005 0 0.0017 0.0546 0 0.0001 0.01 0 0.0013 0.0001 0.0002 0
Nuclear Periphery 0.0046 0.0002 0.0003 0 0.0006 0.001 0.0159 0.0009 0.0034 0.0004 0.0003 0.0064 0.0292 0.0004 0.0068 0.0005 0.0043 0.0007
Nucleolus 0.0219 0.0101 0.0054 0.0058 0.1574 0.0583 0.0106 0.0111 0.0093 0.007 0.0824 0.0553 0.0211 0.0135 0.0066 0.0282 0.165 0.0658
Nucleus 0.8217 0.9895 0.9388 0.9941 0.7855 0.9112 0.8698 0.9877 0.9468 0.9339 0.912 0.9241 0.8473 0.9858 0.9394 0.9371 0.824 0.9177
Peroxisomes 0.0143 0 0.0047 0 0.0026 0.002 0.0034 0 0.0058 0 0 0.0001 0.0049 0 0.0012 0.0002 0 0.0007
Punctate Nuclear 0.0068 0.0001 0.0009 0 0.0008 0.0004 0.0188 0.0001 0.0037 0.0002 0.0003 0.0011 0.0207 0.0001 0.0049 0.0001 0.0011 0.0075
Vacuole 0.0017 0 0.0001 0 0.0006 0.0001 0.006 0 0.0004 0 0.0004 0.0011 0.0028 0.0001 0.0021 0.0035 0.0017 0.0001
Vacuole Periphery 0.0022 0 0.0001 0 0.0003 0.0002 0.003 0 0.0002 0 0.0004 0.0012 0.0018 0 0.0016 0.0033 0.0009 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.3831 33.4182 21.598 18.147 32.052 25.31 30.7917 26.4837 20.3524 29.9403
Translational Efficiency 1.0493 1.1854 0.7331 0.8097 1.0988 1.3437 1.0423 1.2249 1.0941 1.0725

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2193 853 2759 1194 1709 1975 1685 1407 3902 2828 4444 2601

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1062.32 1171.97 1217.98 1279.10 925.14 1101.41 1069.16 1324.71 1002.24 1122.69 1161.55 1303.77
Standard Deviation 164.25 204.29 256.38 248.39 149.91 177.67 222.53 244.77 172.15 188.90 254.55 247.48
Intensity Change Log 2 — 0.141717 0.197272 0.267911 — 0.251608 0.208734 0.517933 — 0.193913 0.202619 0.389698

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000240 0.001044 0.000372 0.000702 0.000398 0.000565 0.000643 0.000815 0.000309 0.000709 0.000475 0.000763
Bud Neck 0.007290 0.018080 0.013838 0.014793 0.005238 0.009795 0.012159 0.022572 0.006391 0.012294 0.013201 0.019001
Bud Site 0.000416 0.001857 0.000689 0.001014 0.000654 0.001588 0.001242 0.002765 0.000520 0.001669 0.000899 0.001961
Cell Periphery 0.000070 0.000102 0.000048 0.000063 0.000062 0.000225 0.000645 0.000133 0.000066 0.000188 0.000274 0.000101
Cytoplasm 0.000025 0.000225 0.000187 0.000417 0.000427 0.000660 0.003135 0.001785 0.000201 0.000529 0.001305 0.001157
Cytoplasmic Foci 0.000003 0.000152 0.000053 0.000884 0.000181 0.000487 0.000781 0.000967 0.000081 0.000386 0.000329 0.000929
Eisosomes 0.000127 0.000079 0.000070 0.000046 0.000064 0.000085 0.000070 0.000054 0.000099 0.000084 0.000070 0.000050
Endoplasmic Reticulum 0.002639 0.003211 0.000797 0.001168 0.002251 0.003122 0.001950 0.001319 0.002469 0.003149 0.001234 0.001250
Endosome 0.000061 0.000430 0.000024 0.000773 0.000181 0.000507 0.000251 0.001266 0.000113 0.000484 0.000110 0.001040
Golgi 0.000016 0.000115 0.000030 0.000775 0.000191 0.000438 0.000089 0.001124 0.000093 0.000341 0.000052 0.000964
Lipid Particles 0.000005 0.000172 0.000003 0.000054 0.000167 0.000121 0.000132 0.000179 0.000076 0.000136 0.000052 0.000121
Mitochondria 0.000453 0.001574 0.000736 0.002038 0.000611 0.000970 0.001138 0.003404 0.000522 0.001152 0.000888 0.002777
Mitotic Spindle 0.000050 0.003051 0.000524 0.004393 0.001270 0.002703 0.001544 0.007122 0.000585 0.002808 0.000911 0.005869
None 0.000463 0.000791 0.000688 0.001392 0.001021 0.003431 0.015692 0.001971 0.000707 0.002635 0.006377 0.001705
Nuclear Periphery 0.000065 0.000168 0.000087 0.000549 0.000069 0.000225 0.000225 0.000995 0.000067 0.000208 0.000139 0.000790
Nuclear Periphery Foci 0.000018 0.000319 0.000024 0.000769 0.000138 0.000407 0.000370 0.000539 0.000071 0.000380 0.000155 0.000645
Nucleolus 0.035470 0.041673 0.020311 0.027737 0.044549 0.048646 0.032695 0.031290 0.039447 0.046543 0.025007 0.029659
Nucleus 0.952067* 0.924963* 0.961005* 0.940122* 0.941175* 0.924131* 0.925852* 0.918729* 0.947296* 0.924382* 0.947677* 0.928550*
Peroxisomes 0.000188 0.001005 0.000292 0.001247 0.000872 0.000787 0.000675 0.000713 0.000488 0.000853 0.000437 0.000958
Vacuole 0.000167 0.000731 0.000114 0.000703 0.000286 0.000700 0.000481 0.001139 0.000219 0.000709 0.000253 0.000939
Vacuole Periphery 0.000167 0.000258 0.000108 0.000361 0.000196 0.000406 0.000230 0.001121 0.000179 0.000362 0.000155 0.000772

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.78 -12.12 -15.12 1.43 -11.08 -3.09 -6.53 -6.76 -4.28 -2.89 -6.66 -9.44 -13.35 -1.83 -9.08
Bud Neck -11.45 -13.45 -9.37 0.63 -2.60 -6.38 -9.69 -12.28 -9.12 -6.84 -10.96 -16.52 -15.19 -8.30 -7.11
Bud Site -3.38 -6.05 -4.34 1.05 -2.78 -2.74 -2.79 -4.44 -2.66 -3.47 -4.60 -4.18 -5.70 -1.78 -4.48
Cell Periphery -1.01 2.11 0.36 1.15 -1.47 -2.54 -2.35 -2.33 1.37 2.03 -2.69 -2.19 -2.06 1.84 1.77
Cytoplasm -2.48 -2.55 -1.83 -1.27 -1.35 -0.41 -3.24 -2.33 -2.22 0.46 -1.29 -3.35 -3.04 -2.32 -0.87
Cytoplasmic Foci -1.94 -2.13 -2.15 -1.65 -2.00 -1.34 -1.65 -2.31 -1.27 -0.10 -2.05 -1.74 -3.27 -1.71 -1.66
Eisosomes 5.12 6.49 9.69 6.45 6.09 -4.33 -1.31 2.40 7.07 3.60 2.75 5.35 9.71 9.72 6.64
Endoplasmic Reticulum -2.25 18.78 11.44 7.35 -6.16 -4.92 1.98 7.63 10.30 6.14 -4.73 16.14 13.56 12.90 -0.66
Endosome -1.34 3.71 -1.65 -0.50 -1.75 -1.94 -0.74 -2.79 -1.74 -2.60 -2.72 -0.03 -3.26 -1.56 -3.28
Golgi -2.57 -7.96 -1.38 -1.15 -1.34 -0.91 0.81 -1.70 -1.05 -1.96 -1.38 0.70 -2.26 -1.33 -2.40
Lipid Particles -1.83 1.34 -2.30 0.95 -2.35 0.34 0.08 -0.51 -0.82 -0.59 -1.16 0.31 -1.21 -0.27 -1.47
Mitochondria -4.88 -2.45 -9.08 -2.35 -9.48 -2.70 -4.91 -7.20 -6.17 -5.68 -5.16 -4.64 -10.09 -7.10 -8.69
Mitotic Spindle -1.96 -1.93 -2.91 -0.41 -2.33 -1.49 -0.75 -3.68 -2.66 -3.30 -2.90 -1.51 -4.85 -2.34 -4.23
None -1.55 -1.35 -3.89 -2.53 -3.12 -3.03 -6.82 -1.72 1.63 6.20 -3.55 -6.83 -3.27 1.52 5.21
Nuclear Periphery -2.77 -1.24 -3.12 -2.37 -2.92 -2.43 -3.46 -3.73 -3.02 -3.04 -3.20 -3.40 -4.77 -3.69 -4.23
Nuclear Periphery Foci -1.91 -0.73 -1.96 -1.04 -1.93 -2.17 -1.44 -2.29 -0.77 -0.74 -3.29 -1.38 -2.94 -1.23 -2.36
Nucleolus -2.18 7.67 0.77 2.36 -3.99 -1.71 4.13 2.36 3.66 -1.10 -3.86 8.86 1.86 4.75 -4.42
Nucleus 6.87 -3.14 4.89 -1.16 6.47 4.69 4.10 6.12 2.68 2.67 8.76 0.85 8.23 1.26 7.55
Peroxisomes -6.99 -4.90 -3.53 -0.65 -3.10 0.25 0.62 -0.06 -0.57 -1.60 -2.66 0.22 -3.08 -1.03 -3.98
Vacuole -3.31 2.74 -1.85 -0.96 -1.92 -2.24 -1.94 -4.66 -2.07 -3.31 -3.76 -1.11 -3.29 -1.68 -3.12
Vacuole Periphery -1.61 3.02 -1.46 -0.59 -1.93 -2.18 -0.52 -3.10 -2.22 -2.93 -2.71 0.83 -3.44 -2.10 -3.58
Standard name
Human Ortholog
Description Component of the NuA3b histone acetyltransferase complex; regulates interaction between NuA3b and H3K36me3 at the transcribed regions of genes; contains PWWP domain; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pdp3

Pdp3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pdp3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available