Standard name
Human Ortholog
Description Monomeric glyoxalase I; catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.97 0.99 0.95 0.88 0.82 0.81 0.79 0.75 0.7 0.66 0.99 0.99 0.99 0.86 0.89 0.87 0.86 0.93 0.81 0.85 0.84 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.06 0 0 0 0.05 0.11 0.06 0.15 0.09 0.25 0.12 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.08 0.12 0.15 0.14 0.18 0.15 0.23 0 0 0 0.11 0.08 0.08 0 0 0.1 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 0 0 1 1 0 0 0 0 0 0 1 0 0 1 3 9
Bud 3 0 0 0 2 4 5 3 8 5 16 12 0 0 0 0 0 0 2 1 0 8 25 27
Bud Neck 0 0 0 1 3 0 4 8 3 3 0 2 0 2 1 0 0 0 1 0 0 0 3 7
Bud Site 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 2 5 7 3 7 11 2 6 10 0 5 3 15 11 7 0 0 0 2 3 4
Cytoplasm 190 34 35 362 488 435 651 585 382 405 292 312 330 688 648 269 210 220 178 55 60 234 513 492
Endoplasmic Reticulum 0 0 1 0 4 0 2 0 2 1 2 2 3 2 0 17 8 10 0 0 0 0 3 3
Endosome 0 0 0 0 1 1 1 1 0 0 0 1 0 3 2 11 5 8 1 0 0 3 3 5
Golgi 0 0 0 0 0 0 0 1 0 0 0 1 1 1 1 3 4 7 0 0 0 0 0 3
Mitochondria 16 2 0 0 3 23 88 40 72 50 105 58 0 1 0 1 3 6 11 0 2 9 14 19
Nucleus 0 0 0 3 3 8 20 23 15 24 9 20 0 0 0 2 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 1 3 1 1 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 2 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 2 3 6 1 2 4 5 0 0 0 2 2 0 3 0 1 6 16 27
Vac/Vac Membrane 0 0 0 2 24 40 97 105 66 99 62 110 2 5 4 33 19 21 1 0 7 4 6 2
Unique Cell Count 203 35 36 365 515 493 795 724 485 543 416 475 334 697 654 312 237 252 208 60 75 277 609 621
Labelled Cell Count 209 36 36 370 533 520 876 781 562 594 499 535 336 707 659 355 265 281 208 60 75 277 609 621


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.0 5.4 5.7 6.1 6.1 5.8 6.7 6.3 6.6 6.7 6.8 5.8 5.9 6.1 9.9 10.3 9.5 5.5 5.5 5.8
Std Deviation (1e-4) 1.2 1.5 1.4 1.9 2.4 2.4 2.3 2.4 2.8 2.6 3.4 3.1 2.1 2.0 2.0 3.0 3.9 2.8 1.9 1.8 1.9
Intensity Change (Log2) – – – 0.07 0.18 0.16 0.09 0.3 0.21 0.28 0.3 0.32 0.1 0.13 0.18 0.87 0.92 0.81 0.01 0.02 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5789 2.5412 1.4556 1.774 1.4092 2.1179 0.3775 2.1627 1.3914 0.7741 1.6083 2.1864 1.1338 3.4523 2.2198 0.9827 1.9049 2.3902
Actin 0.0413 0.0012 0.0203 0.0003 0.0042 0.0009 0.0472 0.0039 0.0112 0.0008 0.016 0.0111 0.0103 0.0002 0.0005 0.0003 0.0046 0.0005
Bud 0.0083 0.0115 0.0033 0.0003 0.0077 0.0101 0.0006 0.0015 0.0033 0.001 0.0002 0.0002 0.001 0.0003 0.0007 0.0001 0 0.0001
Bud Neck 0.0029 0.0003 0.006 0.0004 0.0017 0.001 0.0008 0.0001 0.0003 0.0003 0.0004 0.001 0.0002 0.0001 0.0001 0.0001 0.0005 0.0007
Bud Periphery 0.0057 0.0086 0.0023 0.0002 0.002 0.0045 0.0008 0.0014 0.0032 0.0007 0.0003 0.0001 0.0011 0.0001 0.0005 0 0 0.0001
Bud Site 0.006 0.0223 0.0035 0.0001 0.0023 0.0011 0.0041 0.0048 0.0024 0.0003 0.0006 0.0001 0.0004 0.0009 0.0002 0.0001 0 0
Cell Periphery 0.0005 0.0005 0.0003 0.0001 0.0007 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.3966 0.6219 0.5357 0.6627 0.5543 0.5758 0.3179 0.5488 0.5201 0.5033 0.2709 0.6662 0.6227 0.8562 0.7221 0.687 0.6378 0.8066
Cytoplasmic Foci 0.016 0.0065 0.0101 0.0049 0.007 0.0084 0.0254 0.0034 0.0112 0.0119 0.029 0.0078 0.0046 0.0055 0.004 0.0081 0.0036 0.005
Eisosomes 0.0006 0.0001 0.0001 0.0001 0.0002 0 0.0011 0.0001 0.0001 0 0.0002 0.0001 0.0001 0 0.0001 0 0.0004 0
Endoplasmic Reticulum 0.0048 0.0018 0.0039 0.0018 0.0094 0.0039 0.002 0.0009 0.0014 0.0028 0.0006 0.0013 0.0046 0.0016 0.0016 0.0059 0.0009 0.0023
Endosome 0.0356 0.0016 0.019 0.0019 0.0365 0.0109 0.0175 0.0005 0.0059 0.004 0.012 0.0065 0.0058 0.0015 0.0009 0.0155 0.0009 0.0015
Golgi 0.0185 0.0003 0.0043 0.0001 0.0074 0.0086 0.0072 0.0001 0.0031 0.0003 0.0375 0.0017 0.0013 0.0001 0.0001 0.0003 0.0002 0.0001
Lipid Particles 0.0088 0.0002 0.0052 0.0002 0.0091 0.0001 0.0142 0.0001 0.0058 0.0003 0.0552 0.0014 0.0005 0.0001 0.0002 0.0001 0.0002 0.0001
Mitochondria 0.0056 0.0004 0.0023 0.0001 0.0062 0.0014 0.0073 0.0001 0.0069 0.0005 0.0161 0.0003 0.0006 0.0001 0.0002 0.0001 0.0001 0.0002
None 0.3852 0.3133 0.3661 0.321 0.3039 0.3655 0.5109 0.4307 0.4136 0.4665 0.5 0.2917 0.3386 0.129 0.2649 0.2726 0.3455 0.1764
Nuclear Periphery 0.0244 0.001 0.0042 0.0009 0.0195 0.0009 0.0051 0.0004 0.0012 0.0013 0.0005 0.0013 0.0032 0.0009 0.0006 0.0021 0.0006 0.0008
Nucleolus 0.002 0.0003 0.0005 0.0001 0.0027 0.0001 0.0016 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0 0 0 0.0002 0
Nucleus 0.0055 0.0041 0.0045 0.0028 0.0092 0.0033 0.0048 0.0018 0.0034 0.0021 0.0005 0.0056 0.0024 0.002 0.0019 0.0049 0.0015 0.0046
Peroxisomes 0.0054 0.0003 0.0024 0.0001 0.0006 0.0001 0.0118 0.0001 0.004 0.0003 0.0525 0.0006 0.0001 0 0.0001 0 0.0002 0
Punctate Nuclear 0.0219 0.0021 0.0033 0.0012 0.0046 0.0011 0.0174 0.0005 0.001 0.0016 0.0061 0.0021 0.0006 0.0003 0.0004 0.0006 0.0027 0.0004
Vacuole 0.0027 0.0013 0.002 0.0007 0.0064 0.0017 0.0017 0.0005 0.0013 0.0015 0.0005 0.0007 0.0013 0.0007 0.0007 0.0015 0.0002 0.0005
Vacuole Periphery 0.0017 0.0002 0.0006 0.0001 0.0045 0.0005 0.0006 0.0001 0.0004 0.0004 0.0006 0.0002 0.0003 0.0001 0.0001 0.0005 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 164.7796 119.6522 103.7093 125.455 92.6033 141.6432 136.2298 142.0359 145.0596 114.7536
Translational Efficiency 1.6216 1.57 1.9108 1.6239 1.8891 2.087 1.5857 1.4081 1.4379 1.4887

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1395 735 1911 1210 2354 1938 340 1318 3749 2673 2251 2528

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 662.45 743.21 813.70 1107.96 641.35 746.67 983.06 1213.77 649.20 745.72 839.28 1163.13
Standard Deviation 74.82 114.50 172.02 297.50 80.53 121.59 201.60 397.13 79.11 119.69 186.91 356.90
Intensity Change Log 2 — 0.165958 0.296685 0.742022 — 0.219359 0.616168 0.920311 — 0.192474 0.462675 0.832478

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000088 0.000303 0.000150 0.001034 0.000335 0.000333 0.000531 0.000718 0.000243 0.000325 0.000208 0.000869
Bud Neck 0.011228 0.025656 0.004204 0.007097 0.025539 0.025324 0.002909 0.002196 0.020214 0.025415 0.004009 0.004542
Bud Site 0.003544 0.017074 0.008221 0.030559 0.018966 0.013746 0.006017 0.023048 0.013227 0.014661 0.007888 0.026643
Cell Periphery 0.000107 0.000139 0.000102 0.000217 0.000880 0.000104 0.000233 0.000545 0.000592 0.000114 0.000121 0.000388
Cytoplasm 0.560798* 0.492634* 0.650828* 0.589964* 0.517733* 0.586695* 0.413482* 0.612532* 0.533758* 0.560831* 0.614978* 0.601730*
Cytoplasmic Foci 0.214429* 0.241172* 0.011228 0.017863 0.110345 0.165216 0.026665 0.023200 0.149075 0.186102* 0.013559 0.020645
Eisosomes 0.000107 0.000107 0.000016 0.000032 0.000223 0.000079 0.000084 0.000026 0.000180 0.000087 0.000026 0.000029
Endoplasmic Reticulum 0.000625 0.000648 0.001103 0.001776 0.003156 0.000367 0.003791 0.000763 0.002214 0.000444 0.001509 0.001248
Endosome 0.009935 0.031634 0.001031 0.004827 0.006934 0.012834 0.005079 0.007029 0.008051 0.018003 0.001643 0.005975
Golgi 0.002090 0.004275 0.000108 0.003173 0.001301 0.003029 0.000166 0.004669 0.001595 0.003371 0.000116 0.003953
Lipid Particles 0.006699 0.005721 0.000493 0.001723 0.004648 0.003382 0.003187 0.000808 0.005411 0.004025 0.000900 0.001246
Mitochondria 0.002758 0.004608 0.000498 0.001251 0.002855 0.007656 0.000826 0.000979 0.002819 0.006818 0.000547 0.001109
Mitotic Spindle 0.000642 0.002771 0.007793 0.059050 0.000803 0.004818 0.004294 0.094529 0.000743 0.004255 0.007264 0.077547
None 0.004541 0.004763 0.007629 0.005679 0.007051 0.003807 0.007239 0.008207 0.006117 0.004070 0.007570 0.006997
Nuclear Periphery 0.000309 0.000233 0.000660 0.000769 0.000325 0.000266 0.001432 0.000653 0.000319 0.000257 0.000777 0.000709
Nuclear Periphery Foci 0.000482 0.001038 0.001015 0.003721 0.000446 0.000418 0.004643 0.001998 0.000459 0.000588 0.001563 0.002822
Nucleolus 0.000877 0.001806 0.000280 0.000334 0.001072 0.000976 0.000856 0.000317 0.000999 0.001205 0.000367 0.000325
Nucleus 0.133865* 0.075407 0.269719* 0.151708* 0.198176* 0.096116 0.401836* 0.106849 0.174246* 0.090421 0.289675* 0.128320
Peroxisomes 0.004256 0.014859 0.000216 0.001067 0.002680 0.006829 0.001959 0.001209 0.003267 0.009037 0.000480 0.001141
Vacuole 0.041629 0.073238 0.034529 0.117158 0.095548 0.066735 0.114373 0.100354 0.075485 0.068523 0.046589 0.108397
Vacuole Periphery 0.000993 0.001915 0.000176 0.000999 0.000984 0.001269 0.000399 0.009370 0.000987 0.001447 0.000210 0.005363

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.46 -7.33 -7.01 -4.87 -6.13 0.05 -2.23 -0.21 -0.23 2.16 -0.77 -0.25 -4.10 -2.59 -4.95
Bud Neck -4.73 5.71 3.52 6.56 -2.67 0.28 15.46 15.01 11.52 -2.59 -2.75 15.27 14.31 12.87 -2.69
Bud Site -8.41 -3.66 -9.69 -1.77 -6.44 2.98 6.67 0.67 -1.91 -5.22 -0.98 4.51 -3.95 -3.02 -7.63
Cell Periphery -3.12 0.48 -5.95 -4.26 -6.35 10.31 8.27 6.34 -1.20 0.57 10.00 9.79 6.07 -2.32 -2.08
Cytoplasm 5.22 -4.91 8.14 1.35 13.23 -7.97 9.42 5.03 10.77 -5.67 -3.53 -5.20 8.86 10.71 12.19
Cytoplasmic Foci -2.60 35.91 31.75 25.76 -7.08 -10.08 23.84 23.24 28.41 -1.94 -7.35 44.75 37.75 37.77 -8.58
Eisosomes -0.11 10.86 9.11 13.12 -6.51 10.23 9.40 14.87 13.62 10.54 9.67 16.50 16.85 17.31 1.49
Endoplasmic Reticulum -0.52 -6.71 -7.46 -7.59 -1.04 9.61 -3.19 8.67 -4.95 9.94 9.46 1.41 5.94 -10.08 7.18
Endosome -7.32 10.21 8.05 10.30 -4.70 -6.73 3.30 6.71 11.24 2.80 -9.38 14.99 10.76 15.19 -4.28
Golgi -3.06 4.94 1.93 4.60 -1.93 -3.62 8.83 0.57 3.33 -2.61 -4.34 8.73 1.77 5.07 -3.28
Lipid Particles 0.90 10.03 4.21 2.00 -9.40 2.75 3.50 7.96 3.31 3.43 3.02 14.71 7.34 2.71 -10.19
Mitochondria -1.69 4.39 4.17 4.38 -1.04 -4.36 3.70 4.31 7.82 0.53 -4.78 5.71 5.73 8.80 0.01
Mitotic Spindle -2.33 -5.72 -13.13 -12.62 -11.80 -3.58 -2.39 -17.56 -16.85 -16.84 -4.17 -6.09 -21.80 -20.78 -20.13
None -0.10 -3.52 -1.62 -0.90 2.85 3.70 1.34 -1.06 -7.67 -2.70 3.11 -0.85 -1.24 -6.20 -0.64
Nuclear Periphery 2.45 -13.16 -19.94 -21.77 -8.02 1.43 -15.34 -19.23 -19.06 4.54 2.14 -18.47 -27.61 -27.33 -6.69
Nuclear Periphery Foci -4.36 -7.01 -11.39 -10.18 -9.96 -0.15 -5.90 -12.14 -11.80 1.69 -2.48 -8.92 -15.78 -15.02 -10.10
Nucleolus -2.50 5.83 3.69 3.58 -2.87 0.67 2.93 3.33 3.43 0.36 -1.58 7.36 4.85 5.25 -3.48
Nucleus 10.34 -23.78 -2.81 -12.09 19.82 21.51 -11.96 15.06 -4.02 17.50 22.74 -22.15 10.14 -10.84 28.74
Peroxisomes -5.99 9.30 8.11 8.31 -2.75 -5.34 1.94 3.99 7.56 0.27 -7.72 10.67 8.18 11.45 -1.97
Vacuole -7.45 -5.14 -23.45 -16.87 -20.64 6.98 -7.43 -15.23 -18.89 -1.63 1.43 -0.83 -25.69 -26.01 -23.02
Vacuole Periphery -2.42 2.78 2.13 6.78 -1.84 -1.58 4.65 -1.08 -0.72 -2.03 -2.56 6.05 -0.23 0.86 -2.38
Standard name
Human Ortholog
Description Monomeric glyoxalase I; catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress
Localization
Cell Percentages cytoplasm (55%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Glo1

Glo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Glo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available