Standard name
Human Ortholog
Description Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; relative distribution to the nucleus increases upon DNA replication stress; YAP1 has a paralog, CAD1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.93 0.94 0.86 0.88 0.86 0.77 0.79 0.76 0.7 0.7 0.71 0.91 0.92 0.93 0.91 0.91 0.91 0.87 0.9 0.87 0.91 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.11 0.08 0.15 0.21 0.33 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.06 0.06 0.1 0.1 0.07 0.16 0.1 0.09 0.17 0.05 0.1 0.08 0.09 0.09 0.11 0.16 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 1 0 3 0
Bud 0 0 0 0 0 0 1 4 3 3 2 1 0 0 0 0 1 0 0 4 1 2 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 1
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 1 1 2 4 4 5 3 2 7 0 2 0 11 5 13 0 0 0 0 0 0
Cytoplasm 216 389 226 166 277 304 289 317 192 252 232 235 182 350 362 556 423 369 218 395 242 319 340 383
Endoplasmic Reticulum 2 6 1 1 3 1 0 7 1 1 4 4 2 1 0 15 10 11 2 1 4 1 0 3
Endosome 4 2 2 5 1 3 3 2 2 0 1 3 5 6 9 5 2 5 3 5 0 3 4 9
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 2 4 1 0 1 1
Mitochondria 4 1 1 2 4 23 40 31 38 75 108 74 0 0 2 8 4 6 4 3 6 2 3 6
Nucleus 2 3 0 7 7 12 13 13 12 5 4 11 4 1 1 5 1 1 5 12 2 5 4 4
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 1 3 2 1 2 0 0 1 2 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 2 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 0 2 4 5 6 0 4 0 0 0 0 0 0 2 2 1 2 4 3
Vac/Vac Membrane 13 26 14 20 32 26 58 41 23 63 17 32 16 33 33 70 73 60 3 3 8 5 3 4
Unique Cell Count 232 418 240 193 316 355 374 401 252 362 330 333 199 379 388 612 465 407 250 439 278 352 374 425
Labelled Cell Count 242 429 245 205 325 371 410 425 287 412 372 375 209 393 409 672 522 465 250 439 278 352 374 425


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 7.0 6.9 6.4 7.4 6.5 6.4 6.6 5.9 5.9 5.3 5.6 7.8 7.5 6.8 8.9 9.1 8.7 6.5 6.7 6.8
Std Deviation (1e-4) 0.7 1.0 1.1 1.4 1.6 1.2 1.2 1.4 1.4 1.4 1.1 1.2 1.1 0.9 1.3 1.6 1.7 1.6 0.9 1.2 1.1
Intensity Change (Log2) – – – -0.12 0.1 -0.09 -0.11 -0.05 -0.24 -0.22 -0.38 -0.31 0.17 0.11 -0.01 0.36 0.4 0.33 -0.1 -0.04 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.3
Cytoplasm -2.9 -2.6 -3.3 -5.6 -5.1 -5.6 -7.3 -7.1 -7.0 -1.1 -0.9 -0.4 -1.6 -1.5 -1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.7 5.0 4.1 6.0 7.3 9.7 7.6 0 0 0 0 0 0
Nucleus 0 0 0 2.9 0 3.4 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.7 1.8 0.7 3.6 1.9 1.4 4.2 -0.4 1.6 0.9 1.3 1.2 2.5 3.8 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0301 0.0004 0.0008 0.0306 0.0085 0.0028 0.0083 0.0003 0.0033 0.0007 0.0002 0.0028 0.0003 0 0.0008 0 0 0.0001
Bud 0.0004 0.0025 0.0008 0.0007 0.0003 0.0001 0.0008 0.0004 0.0007 0.0058 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0 0
Bud Neck 0.001 0.0004 0.0016 0.0022 0.0005 0.0041 0.0025 0.001 0.0013 0.0011 0.0007 0.0035 0.0002 0.0001 0.0004 0.0006 0.0003 0.0004
Bud Periphery 0.0003 0.0017 0.001 0.001 0.0004 0.0001 0.0013 0.0002 0.0006 0.0111 0.0001 0.0003 0 0 0.0001 0 0 0
Bud Site 0.0026 0.0038 0.0024 0.0197 0.0005 0.0002 0.0011 0.0085 0.0065 0.0007 0.0002 0.0002 0.0001 0.0008 0.0004 0.0001 0.0001 0
Cell Periphery 0.0001 0.0001 0.0002 0.0007 0.0001 0 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0001 0 0 0
Cytoplasm 0.6767 0.8654 0.7698 0.7828 0.7475 0.8004 0.7132 0.8075 0.7644 0.7496 0.8415 0.7793 0.8751 0.9415 0.6776 0.8811 0.9728 0.9089
Cytoplasmic Foci 0.0478 0.0083 0.0113 0.0138 0.0181 0.0239 0.0143 0.0066 0.0097 0.0186 0.0184 0.0135 0.0033 0.0015 0.016 0.0177 0.0031 0.0012
Eisosomes 0.0001 0 0 0.0002 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0073 0.0038 0.0078 0.0036 0.0036 0.0059 0.0062 0.0025 0.003 0.0024 0.0042 0.0032 0.0459 0.0007 0.0342 0.0033 0.0028 0.0019
Endosome 0.0347 0.0092 0.0154 0.0356 0.0456 0.0129 0.0108 0.0028 0.0059 0.0091 0.01 0.0062 0.004 0.0002 0.0508 0.0069 0.0023 0.0006
Golgi 0.0076 0.0004 0.0063 0.0107 0.035 0.0017 0.0014 0.0002 0.002 0.0005 0.0003 0.0003 0.0001 0 0.0061 0.0001 0 0
Lipid Particles 0.0156 0.0002 0.0004 0.0017 0.0598 0.0012 0.0016 0.0001 0.0004 0.0002 0.0002 0.0005 0.0001 0 0.0019 0.0001 0 0
Mitochondria 0.0036 0.0005 0.0021 0.0027 0.0138 0.0005 0.0008 0.0003 0.0033 0.001 0.0005 0.0004 0.0001 0 0.0007 0.0003 0.0001 0.0001
None 0.0997 0.0507 0.0855 0.0503 0.0301 0.0966 0.2123 0.1302 0.1387 0.1631 0.0496 0.1503 0.0472 0.0287 0.1218 0.0691 0.0048 0.0127
Nuclear Periphery 0.0091 0.0026 0.0048 0.0018 0.0049 0.0039 0.0039 0.0021 0.0045 0.002 0.0035 0.0021 0.0057 0.0008 0.004 0.0019 0.0024 0.0013
Nucleolus 0.001 0.0004 0.0037 0.0003 0.0005 0.0002 0.0005 0.0003 0.0007 0.0004 0.0002 0.0002 0 0 0.0001 0.0001 0 0
Nucleus 0.0146 0.0421 0.0746 0.0339 0.0057 0.0346 0.0125 0.0318 0.0421 0.0261 0.0623 0.027 0.0147 0.0244 0.0808 0.0124 0.0097 0.0722
Peroxisomes 0.03 0.0003 0.0005 0.0008 0.0058 0.0031 0.0009 0.0002 0.0014 0.0006 0.0002 0.0009 0 0 0.0003 0 0 0
Punctate Nuclear 0.014 0.002 0.0046 0.0011 0.0158 0.0054 0.0041 0.0032 0.0089 0.0036 0.0055 0.0074 0.0007 0.0005 0.0007 0.0021 0.0005 0.0003
Vacuole 0.003 0.0048 0.0058 0.0051 0.0021 0.0021 0.0028 0.0017 0.0019 0.0029 0.0022 0.0013 0.0017 0.0004 0.0025 0.0036 0.0007 0.0002
Vacuole Periphery 0.0008 0.0005 0.0006 0.0007 0.0013 0.0003 0.0004 0.0002 0.0004 0.0004 0.0004 0.0002 0.0002 0 0.0006 0.0004 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.7914 37.8793 34.4792 43.8553 36.9923 45.738 50.6833 56.8694 51.6345 45.0857
Translational Efficiency 1.5596 1.5657 1.4348 0.9877 1.076 1.3688 1.0963 1.0732 1.0966 1.1771

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
30 1670 1631 1368 954 2386 696 1032 984 4056 2327 2400

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 642.80 755.06 953.56 978.12 762.70 772.78 937.90 963.86 759.04 765.48 948.88 971.99
Standard Deviation 53.70 104.31 112.64 128.77 89.27 100.27 140.27 139.39 90.77 102.33 121.77 133.63
Intensity Change Log 2 — 0.232221 0.568954 0.605642 — 0.018942 0.298318 0.337708 — 0.120410 0.428417 0.466445

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000189 0.000384 0.000243 0.000686 0.000101 0.000470 0.000331 0.006095 0.000104 0.000435 0.000269 0.003012
Bud Neck 0.002341 0.008638 0.002712 0.003638 0.002810 0.011993 0.002417 0.004821 0.002796 0.010611 0.002624 0.004147
Bud Site 0.001022 0.009786 0.007060 0.029966 0.001607 0.011633 0.006095 0.041509 0.001589 0.010873 0.006772 0.034930
Cell Periphery 0.000277 0.000095 0.000080 0.000090 0.000165 0.000062 0.000162 0.000200 0.000169 0.000075 0.000104 0.000137
Cytoplasm 0.506904* 0.595734* 0.578282* 0.685863* 0.581685* 0.624075* 0.573999* 0.571999* 0.579405* 0.612406* 0.577001* 0.636902*
Cytoplasmic Foci 0.303534* 0.158742 0.008710 0.034416 0.189961* 0.135287 0.027383 0.041402 0.193423* 0.144944 0.014295 0.037420
Eisosomes 0.000316 0.000051 0.000017 0.000019 0.000087 0.000041 0.000055 0.000038 0.000094 0.000045 0.000028 0.000027
Endoplasmic Reticulum 0.000846 0.001880 0.002645 0.000864 0.001302 0.000841 0.001802 0.002020 0.001288 0.001269 0.002393 0.001361
Endosome 0.003514 0.009421 0.001759 0.007611 0.011008 0.008251 0.003383 0.028573 0.010780 0.008733 0.002245 0.016624
Golgi 0.002357 0.003188 0.000096 0.003229 0.001479 0.002949 0.000086 0.018773 0.001505 0.003048 0.000093 0.009913
Lipid Particles 0.018782 0.004910 0.000872 0.001828 0.009619 0.002895 0.001764 0.001268 0.009898 0.003725 0.001139 0.001587
Mitochondria 0.003424 0.003745 0.000476 0.001771 0.006863 0.005686 0.000304 0.009505 0.006758 0.004887 0.000425 0.005097
Mitotic Spindle 0.000047 0.005485 0.007794 0.036984 0.001918 0.018202 0.004516 0.068068 0.001861 0.012966 0.006814 0.050350
None 0.003063 0.006488 0.005459 0.002645 0.004916 0.004405 0.006005 0.003467 0.004860 0.005263 0.005623 0.002998
Nuclear Periphery 0.000385 0.000909 0.002036 0.001232 0.000677 0.000658 0.001085 0.000646 0.000668 0.000761 0.001751 0.000980
Nuclear Periphery Foci 0.000744 0.002348 0.002421 0.004319 0.002044 0.000780 0.002125 0.003187 0.002004 0.001426 0.002332 0.003832
Nucleolus 0.000877 0.001716 0.000196 0.000174 0.001200 0.001188 0.000556 0.000447 0.001190 0.001405 0.000304 0.000291
Nucleus 0.129813 0.118906 0.325799* 0.060450 0.145492* 0.117314 0.264013* 0.039074 0.145014* 0.117970 0.307319* 0.051258
Peroxisomes 0.008423 0.004152 0.000141 0.001004 0.001780 0.004963 0.000528 0.003670 0.001982 0.004629 0.000257 0.002150
Vacuole 0.012511 0.062157 0.053063 0.121021 0.033771 0.046759 0.103191 0.145258 0.033123 0.053099 0.068056 0.131443
Vacuole Periphery 0.000633 0.001264 0.000140 0.002190 0.001514 0.001547 0.000198 0.009982 0.001487 0.001430 0.000157 0.005541

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.57 -1.27 -1.50 -0.02 -0.74 -3.06 -2.44 -2.37 -1.81 -1.86 -4.48 -6.10 -2.93 -1.73 -2.00
Bud Neck -8.18 -1.63 -0.77 8.31 1.03 -8.72 -0.01 -0.53 8.68 -0.52 -10.19 -0.63 -0.27 11.70 0.52
Bud Site -7.18 -4.67 -8.26 -2.92 -4.41 -9.47 -2.94 -7.22 -2.60 -4.27 -11.53 -5.11 -10.74 -3.77 -6.12
Cell Periphery 3.19 3.31 3.77 1.86 4.85 6.59 1.47 3.72 -1.69 2.93 5.78 4.00 5.64 -0.09 3.94
Cytoplasm -1.81 0.28 -1.70 0.50 -8.95 -4.07 4.92 4.11 8.36 -0.97 -3.23 7.96 1.78 6.39 -7.37
Cytoplasmic Foci 4.01 8.30 7.74 27.68 -12.18 8.14 28.76 27.58 27.89 -2.92 7.66 31.92 28.80 39.15 -12.48
Eisosomes 2.27 2.50 2.54 13.17 2.93 11.89 7.64 15.70 5.82 7.72 9.59 11.96 14.17 13.17 6.84
Endoplasmic Reticulum -3.16 -8.71 0.38 6.78 16.95 3.56 -4.52 1.62 -0.12 4.79 0.09 -11.62 3.24 3.79 14.87
Endosome -7.32 2.16 0.43 9.05 -2.23 2.70 8.07 2.08 0.24 -3.65 2.19 9.22 5.26 5.01 -4.51
Golgi -0.57 2.46 1.48 2.68 -2.92 -3.06 5.12 -2.42 -1.27 -3.60 -3.19 5.32 -1.61 0.70 -4.29
Lipid Particles 2.33 2.96 2.95 8.04 -0.37 7.76 9.22 10.08 8.44 4.30 7.17 10.17 10.29 10.71 1.27
Mitochondria -0.14 2.78 2.73 5.02 -0.18 0.55 3.35 2.14 3.49 -2.84 0.96 3.30 2.80 6.43 -2.68
Mitotic Spindle -5.66 -6.85 -7.63 -4.80 -3.42 -7.80 -2.46 -7.71 -3.12 -5.90 -8.10 -5.31 -10.02 -4.55 -6.49
None -2.27 -1.92 0.21 3.34 6.59 0.62 -0.38 2.61 2.01 2.39 -0.41 -0.40 3.08 3.99 6.00
Nuclear Periphery -3.60 -17.26 -3.26 -0.22 11.43 0.26 -9.29 -2.93 -4.07 7.49 -1.09 -18.57 -2.54 -1.86 14.27
Nuclear Periphery Foci -3.14 -5.21 -4.14 -0.73 1.21 4.32 -2.20 -0.74 -4.23 1.29 1.67 -3.65 -1.84 -4.06 1.90
Nucleolus -2.14 1.81 1.79 5.47 0.02 -0.01 4.48 3.23 3.21 -0.52 -1.12 5.62 4.89 6.62 -0.86
Nucleus 0.50 -9.71 2.30 9.95 43.65 4.60 -11.98 14.49 14.35 22.89 4.84 -26.62 14.05 16.82 49.10
Peroxisomes 1.05 2.03 1.95 8.03 -3.53 -5.73 4.46 1.25 6.81 -3.21 -5.79 5.74 3.50 10.75 -4.79
Vacuole -13.59 -17.18 -32.09 -25.28 -20.18 -5.41 -15.50 -29.46 -27.89 -10.80 -9.24 -19.96 -41.42 -38.18 -23.69
Vacuole Periphery -2.63 2.55 -0.09 2.41 -2.54 -0.04 2.70 -0.99 -1.09 -2.83 0.14 2.77 0.08 -0.05 -3.50
Standard name
Human Ortholog
Description Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; relative distribution to the nucleus increases upon DNA replication stress; YAP1 has a paralog, CAD1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (68%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Yap1

Yap1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yap1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available