Standard name
Human Ortholog
Description Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.24 0.49 0.48 0.61 0.53 0.55 – 0.43 0.46 0.37 0.41 0.38 0.52 0.44 0.24 0.21 0.18 0.12 0.14 0.23 0.29 0.33 0.36
Endoplasmic Reticulum 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.44 0.2 0 0.16 0.05 0.45 0.16 – 0.52 0.5 0.53 0.49 0 0.05 0 0.05 0 0.06 0.07 0 0.06 0 0.06 0.07
Nucleus 0.38 0.47 0.41 0.3 0.21 0.18 0.29 – 0.18 0.18 0.19 0.19 0.38 0.31 0.29 0.54 0.52 0.51 0.34 0.53 0.36 0.32 0.19 0.14
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05
Vac/Vac Membrane 0.31 0.28 0.1 0.16 0.17 0 0.08 – 0.06 0.07 0.08 0.08 0.25 0.18 0.33 0.21 0.26 0.34 0.36 0.23 0.26 0.25 0.28 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 1 0 0 4 0 – 6 4 6 5 1 0 0 0 0 1 0 5 2 1 6 5
Bud Neck 0 0 4 0 0 1 2 – 1 6 2 3 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 1 0 – 1 2 2 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Cytoplasm 2 61 73 92 155 147 204 – 129 162 121 169 78 147 176 32 24 24 9 44 48 38 93 100
Endoplasmic Reticulum 1 0 0 0 1 0 0 – 0 0 1 0 0 0 0 8 9 3 0 0 2 0 0 1
Endosome 0 1 0 6 5 1 7 – 1 0 3 7 5 7 21 5 8 4 1 2 1 1 2 9
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 1 0 2 0 0 0 1 1 2 0 1 0
Mitochondria 7 52 2 31 14 126 60 – 157 178 170 202 9 14 8 7 2 8 6 7 11 5 18 19
Nucleus 6 121 61 58 54 49 105 – 56 63 62 78 77 89 117 74 61 70 27 162 75 41 54 40
Nuclear Periphery 0 0 0 0 1 0 1 – 0 0 0 4 0 0 0 1 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 – 0 2 1 0 0 2 0 1 0 0 0 0 0 1 1 1
Peroxisomes 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 6 1 7 – 5 8 1 6 1 0 0 2 1 0 0 6 4 5 14 13
Vac/Vac Membrane 5 71 15 31 43 8 28 – 19 24 25 33 51 51 130 29 31 46 28 71 55 32 80 77
Unique Cell Count 16 256 150 193 255 278 368 303 355 323 410 204 284 400 136 117 136 81 308 212 131 286 282
Labelled Cell Count 21 307 156 219 279 338 415 375 449 394 514 223 310 454 159 136 156 81 308 212 131 286 282


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.0 4.9 4.8 5.4 3.6 4.5 – 4.1 3.9 3.9 3.9 4.9 4.4 5.2 6.1 6.0 5.9 3.9 4.4 4.5
Std Deviation (1e-4) 0.5 0.7 1.1 1.6 1.8 1.3 1.7 – 1.9 1.3 1.5 1.2 1.4 1.2 1.7 1.8 1.3 1.4 0.8 1.2 1.5
Intensity Change (Log2) – – – -0.04 0.12 -0.45 -0.14 – -0.28 -0.35 -0.35 -0.35 -0.0 -0.16 0.07 0.31 0.27 0.25 -0.33 -0.18 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.7 3.5 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7527 -0.5667 -0.8825 -1.0488 -1.3428 -0.5526 -0.0454 0.1107 0.2472 0.0053 0.4906 0.2456 2.3152 2.6363 2.5973 2.4418 2.7606 2.5775
Actin 0.0046 0.0002 0.0004 0.0003 0.0001 0.0002 0.0135 0.0002 0.0108 0.0068 0.0047 0.0059 0.0287 0.0011 0.0113 0.0003 0.0962 0.0055
Bud 0.0001 0 0.0001 0 0 0 0.0004 0.0001 0.0002 0.0001 0.0002 0.0003 0.0003 0.0006 0.001 0.0001 0.0162 0.0002
Bud Neck 0.0015 0.0004 0.0006 0.0004 0.0006 0.0027 0.0034 0.0005 0.0012 0.001 0.0023 0.0031 0.0012 0.0007 0.0006 0.0005 0.0009 0.0033
Bud Periphery 0.0001 0 0.0001 0 0 0.0001 0.0009 0 0.0004 0.0001 0.0002 0.0005 0.0007 0.0024 0.0005 0.0001 0.013 0.0003
Bud Site 0.0003 0.0004 0.0006 0.0001 0 0.0001 0.0022 0.0008 0.001 0.0003 0.0028 0.0004 0.0009 0.0023 0.0018 0.0002 0.0044 0.0005
Cell Periphery 0 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0 0.0004 0.0004 0.0002 0 0.0032 0.0001
Cytoplasm 0.06 0.0817 0.0611 0.0396 0.081 0.0552 0.1051 0.1029 0.1079 0.0913 0.0553 0.0687 0.1086 0.1455 0.1017 0.1412 0.0793 0.1207
Cytoplasmic Foci 0.006 0.0018 0.0068 0.0015 0.0034 0.0025 0.0062 0.0014 0.0036 0.0086 0.0154 0.0042 0.0106 0.0022 0.0187 0.0013 0.0105 0.004
Eisosomes 0.0002 0 0.0001 0.0001 0 0 0.0011 0 0.0001 0.0001 0.0001 0.0001 0.0007 0.0002 0.0002 0 0.0004 0.0001
Endoplasmic Reticulum 0.0027 0.0026 0.0016 0.0006 0.0008 0.0013 0.0059 0.0027 0.0066 0.0019 0.0025 0.0016 0.0077 0.0039 0.0056 0.0039 0.0066 0.0031
Endosome 0.0044 0.0021 0.0032 0.0008 0.0014 0.0019 0.0128 0.0015 0.0073 0.004 0.0155 0.0041 0.0215 0.0067 0.0216 0.0027 0.0071 0.0078
Golgi 0.0007 0 0.0003 0 0 0 0.0019 0 0.0025 0.001 0.002 0.0004 0.0087 0.0005 0.007 0 0.0068 0.0021
Lipid Particles 0.0015 0.0003 0.0016 0.0003 0.0002 0.0005 0.0029 0.0002 0.0038 0.0031 0.0145 0.0008 0.0307 0.0024 0.0084 0.0001 0.0114 0.0059
Mitochondria 0.0019 0.0005 0.0009 0.0004 0.0002 0.0004 0.0031 0.0004 0.0037 0.0009 0.0011 0.0008 0.0068 0.0145 0.0129 0.0024 0.0062 0.0089
None 0.1571 0.185 0.1767 0.2552 0.2469 0.1589 0.1184 0.1446 0.1459 0.1083 0.1549 0.0855 0.0736 0.2279 0.226 0.3536 0.1321 0.2029
Nuclear Periphery 0.0177 0.0334 0.0248 0.0402 0.0087 0.0121 0.0306 0.0181 0.0314 0.0105 0.0292 0.0092 0.0406 0.0168 0.0431 0.0205 0.0258 0.0113
Nucleolus 0.0075 0.0061 0.0093 0.011 0.0072 0.0118 0.0072 0.0053 0.0052 0.007 0.0125 0.0084 0.0059 0.0059 0.0047 0.0031 0.0035 0.007
Nucleus 0.6893 0.6348 0.6687 0.5791 0.5572 0.6896 0.6435 0.68 0.6202 0.6916 0.6233 0.7604 0.5936 0.5422 0.4776 0.4611 0.5283 0.5648
Peroxisomes 0.0003 0.0001 0.0018 0.0001 0 0.0001 0.0014 0 0.0044 0.0049 0.0031 0.0002 0.004 0.0007 0.0059 0.0001 0.0377 0.008
Punctate Nuclear 0.0426 0.048 0.0398 0.0695 0.0916 0.0614 0.036 0.0387 0.0402 0.0568 0.0585 0.0426 0.0435 0.0097 0.0451 0.0058 0.0053 0.0398
Vacuole 0.001 0.0021 0.0011 0.0003 0.0004 0.0008 0.0024 0.0021 0.0027 0.0014 0.0014 0.0023 0.0069 0.0102 0.0036 0.0018 0.0038 0.0032
Vacuole Periphery 0.0005 0.0004 0.0004 0.0003 0.0001 0.0002 0.0008 0.0003 0.0009 0.0003 0.0006 0.0005 0.0043 0.003 0.0025 0.0009 0.0014 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.5304 16.6243 6.8533 13.5516 10.7162 4.1303 12.6066 16.387 13.8178 13.0727
Translational Efficiency 0.9034 1.1206 1.9658 1.0453 1.4852 3.2079 1.159 0.869 1.1161 1.3272

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1344 917 2078 221 1571 2488 258 1579 2915 3405 2336 1800

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 645.33 756.31 863.88 874.13 671.49 766.42 892.93 835.88 659.43 763.70 867.09 840.58
Standard Deviation 79.08 93.89 99.61 102.94 79.00 90.65 102.79 112.86 80.11 91.64 100.38 112.39
Intensity Change Log 2 — 0.228941 0.420794 0.437811 — 0.190769 0.411181 0.315930 — 0.209602 0.415900 0.376947

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000056 0.000266 0.000152 0.000470 0.000610 0.000290 0.000541 0.000292 0.000355 0.000283 0.000195 0.000314
Bud Neck 0.006193 0.012212 0.005225 0.003047 0.003630 0.013558 0.001622 0.007203 0.004812 0.013195 0.004827 0.006693
Bud Site 0.001030 0.004993 0.002653 0.010984 0.002124 0.007494 0.002977 0.007801 0.001620 0.006820 0.002689 0.008192
Cell Periphery 0.000171 0.000180 0.000060 0.000251 0.000187 0.000177 0.000125 0.000156 0.000180 0.000178 0.000067 0.000167
Cytoplasm 0.280658* 0.256325* 0.053192 0.114756* 0.314962* 0.256714* 0.052849 0.167934* 0.299146* 0.256609* 0.053155 0.161405*
Cytoplasmic Foci 0.112431 0.074909 0.000344 0.005204 0.069214 0.036931 0.002774 0.004525 0.089140 0.047159 0.000613 0.004609
Eisosomes 0.000098 0.000061 0.000010 0.000055 0.000056 0.000041 0.000047 0.000025 0.000075 0.000046 0.000014 0.000029
Endoplasmic Reticulum 0.000796 0.003222 0.003179 0.006453 0.000632 0.000973 0.005952 0.003743 0.000708 0.001578 0.003485 0.004076
Endosome 0.002862 0.004941 0.000364 0.001227 0.001692 0.002751 0.000810 0.002832 0.002231 0.003341 0.000413 0.002635
Golgi 0.000926 0.001887 0.000032 0.000064 0.000245 0.001743 0.000068 0.001542 0.000559 0.001782 0.000036 0.001360
Lipid Particles 0.011061 0.006065 0.000168 0.001220 0.004743 0.002511 0.001125 0.001050 0.007656 0.003468 0.000274 0.001071
Mitochondria 0.007134 0.009115 0.000919 0.000898 0.001110 0.006083 0.000565 0.001513 0.003888 0.006900 0.000880 0.001437
Mitotic Spindle 0.000325 0.004694 0.001230 0.003106 0.002966 0.005563 0.000472 0.013647 0.001748 0.005329 0.001146 0.012353
None 0.012291 0.007956 0.002844 0.007988 0.006962 0.007708 0.005601 0.008680 0.009419 0.007774 0.003149 0.008595
Nuclear Periphery 0.000955 0.001900 0.001274 0.001964 0.000843 0.001212 0.000854 0.001986 0.000895 0.001397 0.001227 0.001983
Nuclear Periphery Foci 0.000374 0.000916 0.000163 0.000550 0.000344 0.000408 0.000471 0.001039 0.000358 0.000545 0.000197 0.000979
Nucleolus 0.003823 0.004440 0.000503 0.000676 0.001818 0.002332 0.001421 0.000988 0.002743 0.002899 0.000605 0.000950
Nucleus 0.541782* 0.581777* 0.922752* 0.795532* 0.577437* 0.641345* 0.908778* 0.752171* 0.560998* 0.625303* 0.921208* 0.757495*
Peroxisomes 0.006312 0.005713 0.000068 0.000645 0.001946 0.002590 0.000214 0.000539 0.003959 0.003431 0.000084 0.000552
Vacuole 0.010099 0.017297 0.004720 0.044729 0.008254 0.009051 0.012595 0.021633 0.009105 0.011272 0.005590 0.024469
Vacuole Periphery 0.000623 0.001132 0.000149 0.000181 0.000223 0.000526 0.000138 0.000698 0.000408 0.000689 0.000147 0.000635

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.85 -7.91 -7.09 -3.84 -5.40 0.55 -0.20 0.41 -0.63 2.47 0.19 0.33 -0.22 -1.26 -3.84
Bud Neck -3.30 0.37 1.01 4.60 0.95 -6.97 1.30 -5.09 3.05 -6.89 -7.17 -0.65 -3.96 3.82 -3.59
Bud Site -4.23 -2.29 -2.42 -1.00 -1.84 -4.42 -1.01 -3.64 0.37 -0.55 -6.04 -1.65 -4.87 -0.19 -3.38
Cell Periphery -0.56 8.55 -0.12 0.04 -1.97 0.21 1.04 1.07 2.16 -0.03 0.06 4.22 1.36 2.17 -5.27
Cytoplasm 2.16 31.99 13.95 11.40 -4.64 7.17 27.92 20.00 15.06 -11.48 6.64 47.93 23.22 17.82 -17.68
Cytoplasmic Foci 6.16 25.87 22.40 14.40 -2.53 8.81 20.82 20.54 17.03 -1.82 13.05 33.40 31.61 22.72 -7.38
Eisosomes 5.96 18.45 1.99 -2.30 -9.76 4.07 0.31 6.61 1.69 3.57 8.38 21.04 11.16 2.04 -12.91
Endoplasmic Reticulum -6.07 -11.87 -9.62 -6.54 -6.24 -3.58 -8.29 -17.60 -16.11 2.79 -6.76 -14.64 -19.75 -15.18 -4.68
Endosome -2.52 9.19 3.68 4.07 -6.41 -2.40 4.27 -0.45 0.96 -2.10 -2.86 11.73 0.52 2.19 -3.30
Golgi -1.95 3.91 3.71 4.49 -4.17 -3.02 4.11 -1.17 1.25 -1.54 -3.05 4.86 -0.38 1.75 -1.62
Lipid Particles 4.80 13.47 11.52 7.17 -7.06 3.22 5.28 5.23 4.62 0.52 7.33 14.10 11.56 7.58 -3.32
Mitochondria -0.86 5.33 5.36 4.24 0.21 -6.26 2.32 -1.85 5.74 -4.15 -3.22 5.37 4.36 6.97 -2.89
Mitotic Spindle -2.79 -2.16 -1.51 -0.47 -1.23 -1.71 1.86 -3.80 -2.63 -5.39 -3.36 0.36 -4.87 -2.76 -5.11
None 2.45 6.13 3.26 1.34 -2.41 -0.66 1.83 0.18 0.80 -1.62 1.70 7.80 2.69 1.16 -5.39
Nuclear Periphery -1.94 -7.07 -8.77 -2.56 -6.55 -2.54 -2.40 -13.27 -8.62 -11.69 -2.99 -9.30 -14.77 -7.63 -10.39
Nuclear Periphery Foci -2.35 1.35 -3.69 -0.24 -5.06 -0.41 -2.19 -2.24 -1.83 -1.29 -1.51 1.33 -2.50 -1.58 -2.85
Nucleolus -0.75 5.48 5.18 4.79 -1.44 -1.48 1.88 3.21 4.89 1.04 -0.44 7.02 5.53 6.53 -3.26
Nucleus -2.86 -42.32 -9.32 -7.09 10.09 -6.65 -25.04 -17.43 -12.37 11.60 -8.32 -56.17 -22.41 -15.26 23.83
Peroxisomes 0.54 7.12 6.45 6.17 -1.42 -1.23 4.71 3.93 6.56 -1.38 0.97 8.60 7.56 8.94 -2.53
Vacuole -5.02 4.34 -8.16 -7.50 -8.45 -0.74 -3.45 -12.99 -12.80 -4.23 -3.27 1.37 -14.91 -13.95 -15.00
Vacuole Periphery -1.82 4.01 3.88 3.78 -0.39 -4.60 1.69 -1.80 0.55 -2.39 -2.90 4.21 -0.10 2.01 -2.45
Standard name
Human Ortholog
Description Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
Localization
Cell Percentages nucleus (26%), cytoplasm (7%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rad33

Rad33


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad33-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available