Standard name
Human Ortholog
Description Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.98 – 0.98 0.98 0.93 0.9 0.93 0.81 0.83 0.68 0.81 0.96 0.97 0.98 0.95 0.92 0.9 0.77 0.84 0.61 0.98 0.87 0.89
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 – 0 0 0.08 0.06 0.05 0.22 0.18 0.43 0.17 0 0 0 0 0 0 0.1 0 0.16 0 0 0
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.07 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 3 3 0 0 1
Bud 3 0 – 1 0 0 1 0 2 1 4 1 1 0 0 0 0 0 0 1 0 0 1 3
Bud Neck 1 1 – 2 5 2 8 12 5 10 5 7 0 0 1 0 2 0 0 1 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 – 6 0 2 2 1 1 1 1 1 0 0 2 4 3 4 0 0 0 0 0 0
Cytoplasm 190 197 – 160 206 205 264 253 152 221 114 164 46 130 249 155 181 266 168 183 30 34 80 138
Endoplasmic Reticulum 3 1 – 0 0 1 0 0 2 0 1 0 0 1 1 10 10 16 1 0 0 0 0 0
Endosome 0 1 – 2 1 1 1 2 1 1 0 3 0 0 1 1 3 10 3 1 0 0 2 1
Golgi 1 1 – 0 1 1 1 1 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0
Mitochondria 15 0 – 1 2 17 19 15 41 49 73 35 1 0 0 3 5 9 22 9 8 0 2 3
Nucleus 0 1 – 0 0 0 3 2 0 0 1 2 1 0 2 0 0 2 0 0 0 0 0 0
Nuclear Periphery 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 – 0 1 1 7 5 7 4 3 11 0 1 3 0 1 1 1 1 0 0 0 0
Vac/Vac Membrane 0 0 – 0 2 5 8 0 3 6 1 2 0 1 1 10 11 21 8 8 2 0 1 1
Unique Cell Count 203 202 164 211 221 294 273 188 265 168 203 48 134 255 163 197 296 218 217 50 35 93 156
Labelled Cell Count 214 206 172 218 235 314 291 215 294 203 226 49 134 261 183 217 330 218 217 50 35 93 156


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.2 – 4.8 5.4 4.2 4.2 4.7 3.8 3.6 3.3 3.6 4.4 5.5 4.9 5.2 5.4 5.2 – 4.8 5.0
Std Deviation (1e-4) 0.5 0.7 – 1.6 2.1 1.6 1.4 2.1 1.5 1.2 1.1 1.4 1.2 1.8 1.4 0.9 1.3 1.5 – 1.4 1.6
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2033 -0.7236 -0.7949 -0.8109 -0.8198 -0.7955 -1.2465 -0.7467 -0.7707 -0.5743 -0.6067 -0.3679 -2.0535 -1.9817 -2.1 -1.9513 -1.7313 -2.2136
Actin 0.0003 0.0013 0.0053 0.0001 0.0002 0.0014 0.0104 0.0027 0.0165 0.0007 0.0386 0.0026 0.0228 0.0001 0.0009 0.0102 0.0045 0.0001
Bud 0.0011 0.0004 0.0012 0.0005 0.0001 0.0003 0.0003 0.0042 0.0008 0.0003 0.0003 0.0001 0.0003 0 0.0001 0.0024 0.0004 0
Bud Neck 0.0005 0.0004 0.0005 0.0001 0.0003 0.0011 0.0003 0.0001 0.0003 0.0002 0.0009 0.0004 0.0145 0 0 0.0001 0.0002 0.0001
Bud Periphery 0.0014 0.0004 0.0014 0.0003 0.0001 0.0002 0.0004 0.0063 0.0013 0.001 0.0003 0.0002 0.0003 0 0.001 0.0035 0.0005 0
Bud Site 0.004 0.0049 0.0009 0 0.0004 0.0007 0.006 0.0054 0.0046 0.0033 0.0085 0.0003 0.0038 0.0001 0.0001 0.0025 0.0002 0
Cell Periphery 0.0001 0.0001 0.0001 0 0.0001 0 0.0003 0.0001 0.0002 0.0005 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0001 0
Cytoplasm 0.0239 0.0459 0.0502 0.0244 0.073 0.0658 0.0211 0.0458 0.0312 0.0125 0.0101 0.0205 0.0217 0.0411 0.0234 0.0276 0.0445 0.0726
Cytoplasmic Foci 0.0092 0.0074 0.01 0.0021 0.0068 0.0066 0.0114 0.0025 0.0035 0.0021 0.008 0.0107 0.0323 0.0055 0.0153 0.0893 0.0104 0.0019
Eisosomes 0.0003 0.0005 0.0003 0 0.0001 0.0001 0.0005 0.0002 0.0003 0.0006 0.0005 0.0001 0.0003 0.0002 0.0002 0.0002 0.0001 0
Endoplasmic Reticulum 0.0004 0.0013 0.0009 0.0002 0.0017 0.0007 0.0038 0.0005 0.0004 0.0022 0.0029 0.001 0.0009 0.0003 0.0004 0.0008 0.0011 0.0002
Endosome 0.0007 0.0011 0.0017 0.0002 0.0032 0.0015 0.0195 0.0011 0.0035 0.0115 0.0097 0.0339 0.0079 0.0002 0.0034 0.0178 0.0312 0.0002
Golgi 0.0002 0.0002 0.0005 0 0.0002 0.0004 0.0031 0.0015 0.0035 0.0008 0.0069 0.0169 0.0043 0 0.0036 0.0143 0.0223 0
Lipid Particles 0.0007 0.0017 0.0014 0.0001 0.0041 0.0007 0.0037 0.0003 0.0019 0.0076 0.0048 0.0158 0.0051 0.0003 0.0061 0.0725 0.005 0.0001
Mitochondria 0.0009 0.0006 0.0007 0.0001 0.0007 0.0002 0.0035 0.0007 0.0103 0.0098 0.0016 0.019 0.003 0.0001 0.0012 0.015 0.0089 0.0001
None 0.9449 0.9256 0.9088 0.9654 0.8849 0.8974 0.896 0.9237 0.9153 0.918 0.9015 0.8614 0.8533 0.951 0.9259 0.6986 0.7982 0.9231
Nuclear Periphery 0.0005 0.0009 0.0026 0.0005 0.0063 0.0025 0.008 0.0003 0.0003 0.0054 0.0012 0.0012 0.0086 0.0001 0.0003 0.0015 0.0004 0.0002
Nucleolus 0.0013 0.0005 0.0004 0.0004 0.0037 0.0004 0.0004 0.0001 0.0001 0.0044 0.0002 0.0002 0.001 0.0001 0.0001 0.0073 0.0001 0.0001
Nucleus 0.0046 0.0022 0.0058 0.0024 0.0044 0.0076 0.0018 0.0034 0.0004 0.0081 0.0004 0.0006 0.0018 0.0003 0.0002 0.0015 0.0004 0.0005
Peroxisomes 0.0016 0.0016 0.0016 0.0001 0.0005 0.0002 0.005 0.0001 0.0048 0.0006 0.0015 0.0109 0.0082 0.0001 0.0169 0.0264 0.0695 0
Punctate Nuclear 0.003 0.0025 0.0051 0.0027 0.0079 0.0118 0.0008 0.0006 0.0004 0.0015 0.001 0.001 0.0082 0.0002 0.0004 0.0053 0.0006 0.0007
Vacuole 0.0005 0.0005 0.0006 0.0002 0.001 0.0002 0.0024 0.0003 0.0004 0.0069 0.0006 0.0017 0.0007 0.0002 0.0002 0.0013 0.0008 0.0001
Vacuole Periphery 0.0001 0.0001 0.0001 0 0.0004 0.0001 0.0013 0 0.0002 0.002 0.0003 0.0013 0.0009 0 0.0001 0.0018 0.0006 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.6033 9.4447 7.8844 10.0258 10.2737 9.2394 8.3558 11.4806 7.5698 11.3744
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
870 129 169 372 1542 909 165 1570 2412 1038 334 1942

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 616.42 778.50 863.94 844.92 663.96 664.91 883.47 810.73 646.81 679.03 873.59 817.28
Standard Deviation 85.38 112.75 110.01 119.42 75.08 92.63 112.74 122.43 82.18 102.46 111.79 122.60
Intensity Change Log 2 — 0.336783 0.487017 0.454901 — 0.002063 0.412085 0.288125 — 0.172909 0.448646 0.370826

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000088 0.000912 0.001433 0.003222 0.000138 0.000225 0.000323 0.002210 0.000120 0.000310 0.000885 0.002404
Bud Neck 0.020078 0.026458 0.004286 0.023993 0.011019 0.033284 0.004392 0.012309 0.014287 0.032435 0.004338 0.014547
Bud Site 0.005910 0.019953 0.003075 0.047169 0.003209 0.015002 0.002248 0.040158 0.004184 0.015617 0.002666 0.041501
Cell Periphery 0.000159 0.000186 0.000293 0.000284 0.000169 0.000306 0.000169 0.000249 0.000166 0.000291 0.000231 0.000256
Cytoplasm 0.497509* 0.301925* 0.111883* 0.337586* 0.503171* 0.343353* 0.255858* 0.410844* 0.501129* 0.338205* 0.183009* 0.396811*
Cytoplasmic Foci 0.171984 0.221476* 0.006966 0.026220 0.166178 0.162019 0.007367 0.015274 0.168272 0.169408 0.007164 0.017371
Eisosomes 0.000194 0.000156 0.000104 0.000066 0.000125 0.000202 0.000079 0.000055 0.000150 0.000197 0.000092 0.000057
Endoplasmic Reticulum 0.000345 0.002314 0.018202 0.003701 0.000580 0.000791 0.006599 0.002340 0.000495 0.000980 0.012470 0.002601
Endosome 0.007487 0.023315 0.003420 0.020205 0.005226 0.021633 0.002722 0.008227 0.006042 0.021842 0.003075 0.010521
Golgi 0.001140 0.004527 0.000141 0.002302 0.001154 0.005222 0.000066 0.004289 0.001149 0.005135 0.000104 0.003908
Lipid Particles 0.005364 0.005260 0.003188 0.001827 0.006645 0.016927 0.002479 0.000979 0.006183 0.015477 0.002838 0.001141
Mitochondria 0.001486 0.011938 0.000706 0.003647 0.003330 0.015561 0.000610 0.005024 0.002665 0.015111 0.000659 0.004760
Mitotic Spindle 0.004635 0.016822 0.000468 0.076160 0.002270 0.020417 0.000465 0.048230 0.003123 0.019970 0.000466 0.053580
None 0.009888 0.002483 0.004265 0.008834 0.004601 0.007971 0.007036 0.008898 0.006508 0.007289 0.005634 0.008886
Nuclear Periphery 0.000346 0.000923 0.002125 0.002074 0.000492 0.001120 0.002800 0.002141 0.000439 0.001095 0.002458 0.002128
Nuclear Periphery Foci 0.000296 0.001251 0.002569 0.002573 0.000310 0.000449 0.001391 0.000939 0.000305 0.000549 0.001987 0.001252
Nucleolus 0.001075 0.001830 0.001737 0.000780 0.001495 0.004088 0.000508 0.000589 0.001344 0.003808 0.001130 0.000626
Nucleus 0.219055* 0.204797* 0.770373* 0.258812* 0.259091* 0.256849* 0.602545* 0.347920* 0.244650* 0.250380* 0.687464* 0.330851*
Peroxisomes 0.003615 0.037771 0.000522 0.002843 0.003888 0.012201 0.000231 0.001575 0.003789 0.015379 0.000378 0.001818
Vacuole 0.048966 0.112816 0.063969 0.173988 0.026058 0.079298 0.101913 0.083589 0.034321 0.083464 0.082714 0.100906
Vacuole Periphery 0.000381 0.002888 0.000278 0.003714 0.000849 0.003083 0.000200 0.004161 0.000681 0.003059 0.000239 0.004075

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.55 -8.29 -2.61 -0.92 0.98 -1.61 -5.31 -2.69 -2.43 -1.31 -3.69 -9.15 -3.41 -2.76 0.39
Bud Neck -0.50 6.01 2.40 1.68 -1.78 -7.12 5.04 0.31 7.41 -5.56 -6.12 7.73 2.54 7.49 -5.46
Bud Site -2.48 0.98 -3.87 -1.00 -4.38 -5.05 -0.68 -9.00 -2.63 -8.79 -5.15 0.27 -9.38 -2.84 -9.74
Cell Periphery -1.01 -5.68 -0.74 0.15 4.21 -3.72 -0.14 0.59 3.99 0.65 -4.04 -4.43 0.20 3.97 4.20
Cytoplasm 8.33 25.38 16.29 1.38 -7.91 13.86 18.90 18.23 1.51 -8.96 16.12 31.03 23.28 2.42 -14.59
Cytoplasmic Foci -1.77 27.04 24.67 10.55 -4.65 0.56 34.20 33.33 23.27 -3.49 0.10 43.61 41.84 25.42 -5.41
Eisosomes 1.49 3.61 7.55 4.21 3.88 -4.08 0.39 8.18 7.93 5.77 -2.82 2.45 11.21 8.33 7.53
Endoplasmic Reticulum -5.44 -12.60 -11.32 -4.21 9.48 -1.19 -10.14 -12.65 -14.38 6.86 -3.93 -14.84 -17.61 -15.16 11.58
Endosome -3.61 2.69 -0.04 3.11 -1.29 -8.12 2.64 1.23 8.71 -1.14 -8.53 3.41 1.45 9.02 -1.16
Golgi -1.82 4.83 1.28 2.11 -1.64 -3.70 5.33 -0.70 2.86 -2.46 -4.01 6.91 -0.56 3.29 -2.57
Lipid Particles -0.05 1.61 4.37 4.27 3.79 -4.83 5.12 8.08 7.86 5.55 -5.09 4.86 9.53 8.02 7.46
Mitochondria -1.54 2.60 -0.05 1.53 -1.35 -4.84 3.88 0.72 5.19 -3.82 -5.37 4.29 0.29 5.46 -3.94
Mitotic Spindle -1.95 0.01 -4.85 -1.29 -4.52 -4.80 -0.65 -8.75 -2.39 -7.80 -5.00 -0.39 -9.79 -2.90 -8.13
None 3.92 3.19 1.45 -2.22 -1.56 -1.82 -0.13 -1.23 1.11 -0.92 -0.50 2.50 0.51 0.81 -1.98
Nuclear Periphery -4.60 -10.65 -12.28 -6.63 -1.16 -3.13 -11.23 -17.88 -9.55 1.07 -3.98 -15.14 -21.17 -10.66 -0.42
Nuclear Periphery Foci -1.95 -4.99 -6.93 -1.35 1.91 -1.33 -4.14 -6.46 -4.65 2.59 -2.45 -6.31 -9.55 -4.93 4.08
Nucleolus -1.56 -1.87 3.01 2.44 3.51 -3.12 2.72 2.41 4.92 -1.56 -3.62 1.06 2.98 5.15 2.93
Nucleus -0.08 -23.16 -3.84 -2.19 17.59 0.52 -12.14 -13.71 -11.78 6.52 -0.53 -23.38 -15.22 -11.24 15.25
Peroxisomes -3.39 3.63 2.97 3.72 -0.79 -5.65 6.90 5.91 8.40 -3.71 -6.56 7.48 6.55 8.70 -2.60
Vacuole -5.14 -4.27 -17.30 -8.60 -10.80 -11.18 -9.98 -23.64 -11.99 2.58 -10.90 -10.61 -27.51 -15.37 -2.66
Vacuole Periphery -1.88 0.89 -1.06 1.04 -1.15 -3.27 1.83 -1.27 1.77 -2.83 -4.08 1.82 -1.74 2.06 -2.95
Standard name
Human Ortholog
Description Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
Localization
Cell Percentages cytoplasm (31%), nucleus (4%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nse5

Nse5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nse5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available