Standard name | |
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Human Ortholog | |
Description | Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; phosphorylated by Cdc28p; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 1 | 3 | 1 | 3 | 2 |
Bud | 1 | 1 | 0 | 4 | 4 | 4 | 26 | 10 | 12 | 18 | 21 | 12 | 0 | 0 | 0 | – | 0 | 0 | 21 | 7 | 18 | 1 | 6 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 2 |
Bud Site | 2 | 0 | 0 | 0 | 3 | 0 | 5 | 11 | 2 | 13 | 9 | 8 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 2 | 0 | 4 | 1 | 0 | 3 | 1 | 1 | 1 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 3 |
Cytoplasm | 227 | 80 | 228 | 211 | 177 | 275 | 331 | 335 | 159 | 240 | 149 | 225 | 156 | 294 | 364 | – | 63 | 30 | 160 | 61 | 123 | 157 | 326 | 370 |
Endoplasmic Reticulum | 3 | 0 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | – | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 3 | 8 | – | 1 | 1 | 3 | 1 | 4 | 1 | 1 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Mitochondria | 60 | 15 | 17 | 54 | 53 | 173 | 360 | 266 | 217 | 293 | 275 | 338 | 0 | 1 | 1 | – | 5 | 1 | 19 | 3 | 47 | 3 | 4 | 10 |
Nucleus | 57 | 18 | 73 | 58 | 79 | 108 | 150 | 172 | 91 | 101 | 68 | 95 | 51 | 67 | 17 | – | 13 | 5 | 66 | 21 | 85 | 3 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 1 | 2 | 1 | 2 | 4 | 4 | 11 | 9 | 14 | 10 | 8 | 4 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 3 | 2 | 1 | 3 | 8 | 14 | 4 | 6 | 9 | 7 | 0 | 0 | 2 | – | 1 | 0 | 23 | 4 | 22 | 1 | 6 | 8 |
Vac/Vac Membrane | 11 | 4 | 12 | 9 | 11 | 5 | 7 | 9 | 7 | 5 | 5 | 8 | 7 | 20 | 22 | – | 8 | 8 | 12 | 3 | 11 | 2 | 2 | 3 |
Unique Cell Count | 318 | 106 | 319 | 303 | 284 | 455 | 666 | 668 | 386 | 495 | 382 | 522 | 211 | 377 | 400 | 85 | 46 | 324 | 107 | 331 | 179 | 371 | 432 | |
Labelled Cell Count | 363 | 121 | 338 | 346 | 334 | 580 | 901 | 837 | 512 | 688 | 546 | 700 | 214 | 385 | 415 | 98 | 50 | 324 | 107 | 331 | 179 | 371 | 432 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.2 | 3.9 | 4.1 | 3.9 | 4.0 | 3.2 | 3.1 | 3.1 | 3.1 | 2.8 | 2.6 | 2.6 | 5.3 | 5.2 | 5.1 | – | 5.9 | 6.4 | 5.2 | 5.0 | 5.3 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.4 | 1.9 | 1.9 | 1.1 | 1.1 | 1.2 | 1.4 | 1.1 | 1.3 | 1.0 | 1.3 | 1.0 | 1.5 | – | 2.0 | 2.6 | 1.7 | 1.5 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.02 | -0.37 | -0.4 | -0.39 | -0.41 | -0.53 | -0.63 | -0.64 | 0.37 | 0.35 | 0.33 | – | 0.53 | 0.66 | 0.34 | 0.29 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.45 | 1.0705 | 0.6513 | 0.0765 | 0.0465 | 0.3633 | -0.5067 | 0.7498 | 0.6245 | -0.0449 | -0.453 | -0.3284 | -1.5769 | -0.1526 | -0.4383 | -0.9535 | -1.3099 | -1.373 |
Actin | 0.0187 | 0.0002 | 0.0002 | 0.0076 | 0.0031 | 0.003 | 0.0285 | 0.0006 | 0.0093 | 0.0258 | 0.0071 | 0.0032 | 0.0155 | 0.0003 | 0.0106 | 0.0258 | 0.0187 | 0.0034 |
Bud | 0.0135 | 0.0078 | 0.002 | 0.0018 | 0.0016 | 0.002 | 0.0171 | 0.013 | 0.0025 | 0.0064 | 0.0211 | 0.0047 | 0.0183 | 0.0014 | 0.0028 | 0.0031 | 0.0007 | 0.0048 |
Bud Neck | 0.0033 | 0.0007 | 0.0004 | 0.0003 | 0.0027 | 0.006 | 0.0144 | 0.0014 | 0.0043 | 0.0036 | 0.0021 | 0.0049 | 0.0075 | 0.0006 | 0.0013 | 0.0012 | 0.0009 | 0.0062 |
Bud Periphery | 0.0149 | 0.0072 | 0.0013 | 0.0038 | 0.0012 | 0.0034 | 0.0235 | 0.0122 | 0.0034 | 0.0073 | 0.0143 | 0.0099 | 0.0236 | 0.0008 | 0.0034 | 0.0019 | 0.0008 | 0.0066 |
Bud Site | 0.017 | 0.0099 | 0.0017 | 0.0006 | 0.0144 | 0.0005 | 0.0267 | 0.0196 | 0.0028 | 0.0091 | 0.0019 | 0.0009 | 0.0344 | 0.0055 | 0.0028 | 0.0023 | 0.0011 | 0.0015 |
Cell Periphery | 0.0018 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0027 | 0.0008 | 0.0003 | 0.0006 | 0.0003 | 0.0004 | 0.0035 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0004 |
Cytoplasm | 0.0505 | 0.0282 | 0.063 | 0.1179 | 0.0833 | 0.0628 | 0.0709 | 0.0672 | 0.0635 | 0.251 | 0.2085 | 0.1058 | 0.0602 | 0.0865 | 0.1139 | 0.1609 | 0.2918 | 0.1439 |
Cytoplasmic Foci | 0.0356 | 0.0029 | 0.0023 | 0.0104 | 0.0064 | 0.0064 | 0.0173 | 0.0056 | 0.0088 | 0.0215 | 0.018 | 0.0139 | 0.0403 | 0.0016 | 0.0077 | 0.0247 | 0.0149 | 0.017 |
Eisosomes | 0.0005 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0037 | 0.0006 | 0.0008 | 0.0025 | 0.0014 | 0.002 | 0.0054 | 0.0022 | 0.0014 | 0.0021 | 0.0032 | 0.0021 | 0.0107 | 0.0024 | 0.0015 | 0.0013 | 0.003 | 0.003 |
Endosome | 0.0153 | 0.0004 | 0.0014 | 0.0279 | 0.0044 | 0.0023 | 0.0243 | 0.0019 | 0.0159 | 0.0345 | 0.0257 | 0.0064 | 0.0409 | 0.0015 | 0.0065 | 0.0228 | 0.0329 | 0.0081 |
Golgi | 0.0034 | 0 | 0 | 0.0097 | 0.001 | 0.0005 | 0.0089 | 0.0002 | 0.0066 | 0.0156 | 0.005 | 0.0041 | 0.0163 | 0 | 0.0036 | 0.0143 | 0.017 | 0.0024 |
Lipid Particles | 0.0097 | 0.0001 | 0.0001 | 0.0072 | 0.0011 | 0.0004 | 0.0205 | 0.0024 | 0.0147 | 0.0123 | 0.015 | 0.0057 | 0.0181 | 0.0002 | 0.0039 | 0.0143 | 0.0206 | 0.0044 |
Mitochondria | 0.0077 | 0.0008 | 0.0004 | 0.0144 | 0.0013 | 0.0011 | 0.0173 | 0.0011 | 0.0183 | 0.0088 | 0.0289 | 0.0124 | 0.0423 | 0.0004 | 0.0083 | 0.0612 | 0.0205 | 0.0095 |
None | 0.3672 | 0.2383 | 0.2758 | 0.6848 | 0.6072 | 0.4219 | 0.3494 | 0.225 | 0.1289 | 0.3557 | 0.4842 | 0.4477 | 0.3158 | 0.236 | 0.1338 | 0.4733 | 0.4896 | 0.3828 |
Nuclear Periphery | 0.0101 | 0.0047 | 0.0069 | 0.0198 | 0.0043 | 0.0095 | 0.0233 | 0.0077 | 0.0115 | 0.0071 | 0.0117 | 0.0056 | 0.0156 | 0.0143 | 0.0079 | 0.0027 | 0.0066 | 0.0083 |
Nucleolus | 0.045 | 0.0097 | 0.0062 | 0.0012 | 0.0142 | 0.0289 | 0.04 | 0.0102 | 0.0077 | 0.0065 | 0.0047 | 0.0128 | 0.041 | 0.0068 | 0.0067 | 0.0012 | 0.0007 | 0.0127 |
Nucleus | 0.3083 | 0.652 | 0.6196 | 0.0631 | 0.2078 | 0.4178 | 0.2426 | 0.6067 | 0.6599 | 0.1976 | 0.1087 | 0.3318 | 0.2018 | 0.6299 | 0.6612 | 0.172 | 0.0377 | 0.3558 |
Peroxisomes | 0.0137 | 0.0003 | 0.0001 | 0.0021 | 0.0009 | 0.0002 | 0.0234 | 0.0019 | 0.007 | 0.0059 | 0.0138 | 0.003 | 0.0086 | 0 | 0.0074 | 0.0115 | 0.0096 | 0.0041 |
Punctate Nuclear | 0.0351 | 0.0341 | 0.0162 | 0.0207 | 0.0415 | 0.0271 | 0.0106 | 0.0163 | 0.0266 | 0.0159 | 0.0186 | 0.0173 | 0.0243 | 0.0096 | 0.0136 | 0.003 | 0.0285 | 0.0182 |
Vacuole | 0.0218 | 0.0015 | 0.0009 | 0.0021 | 0.0016 | 0.0029 | 0.0271 | 0.0035 | 0.0048 | 0.0104 | 0.0048 | 0.0056 | 0.0526 | 0.0016 | 0.0022 | 0.0016 | 0.0029 | 0.0052 |
Vacuole Periphery | 0.003 | 0.0003 | 0.0002 | 0.0019 | 0.0003 | 0.0007 | 0.0058 | 0.0004 | 0.0015 | 0.0022 | 0.0021 | 0.0019 | 0.0086 | 0.0003 | 0.0005 | 0.0007 | 0.0012 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.9206 | 19.5912 | 6.3207 | 25.0396 | 30.8854 | 45.7117 | 12.9187 | 6.3941 | 15.0196 | 44.5288 |
Translational Efficiency | 2.0187 | 1.7189 | 1.4792 | 1.5181 | 2.8456 | 2.3841 | 1.7016 | 2.2851 | 1.6943 | 1.8738 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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