Standard name
Human Ortholog
Description Thioredoxin peroxidase; acts as both ribosome-associated and free cytoplasmic antioxidant; self-associates to form high-molecular weight chaperone complex under oxidative stress; chaperone activity essential for growth in zinc deficiency; required for telomere length maintenance; binds and modulates Cdc19p activity; protein abundance increases, forms cytoplasmic foci during DNA replication stress; TSA1 has a paralog, TSA2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 1.0 0.99 0.98 0.94 0.93 0.94 0.94 0.91 0.86 0.99 0.99 0.99 0.94 0.92 0.92 0.96 0.95 0.95 0.92 0.84 0.64
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0 0 0.05 0.09 0 0 0 0.06 0.05 0.14 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 18
Bud 0 0 0 0 1 2 0 0 2 1 0 1 0 0 1 0 0 0 0 0 1 1 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 3 3 8 6 9 8 13 20 11 17 2 6 8 1 0 1 0 0 0 0 0 0
Cytoplasm 240 195 153 237 281 186 297 351 291 346 286 312 291 508 506 151 67 54 220 173 144 246 354 280
Endoplasmic Reticulum 0 0 1 0 0 2 7 19 11 14 17 34 0 4 0 9 4 8 1 0 0 4 11 21
Endosome 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14 31
Golgi 0 0 0 0 0 0 1 1 0 0 0 0 1 0 1 0 1 1 0 0 0 2 12 38
Mitochondria 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1 1 0 1 0 1 1 0
Nucleus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3
Nucleolus 0 1 1 0 0 0 2 0 0 0 1 1 2 4 3 5 2 5 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 8
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 6
Vac/Vac Membrane 0 1 0 0 0 0 4 0 0 0 1 0 0 1 0 0 2 0 2 1 1 3 2 4
Unique Cell Count 240 197 155 237 285 190 316 376 310 370 314 361 294 515 512 160 73 59 229 183 153 268 423 440
Labelled Cell Count 241 197 158 240 291 196 320 381 317 381 317 365 296 523 519 168 77 70 229 183 153 268 423 440


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 254.6 235.2 210.1 221.5 244.0 180.2 217.7 205.2 207.8 205.7 179.9 197.7 303.6 332.8 360.4 307.3 281.5 316.5 247.8 268.9 288.8
Std Deviation (1e-4) 41.3 70.1 40.9 38.1 55.6 63.6 63.2 56.0 53.9 57.8 60.7 51.0 61.1 72.9 74.5 96.1 101.5 110.5 51.7 64.2 75.9
Intensity Change (Log2) – – – 0.08 0.22 -0.22 0.05 -0.03 -0.02 -0.03 -0.22 -0.09 0.53 0.66 0.78 0.55 0.42 0.59 0.24 0.36 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1.3 1.8 0 1.5 0 0 0 0 0 0
Cytoplasm 1.8 -0.1 -0.6 -2.3 -2.6 -2.4 -2.5 -3.2 -4.3 0.3 -0.1 0.1 -2.1 -2.7 -2.6
Endoplasmic Reticulum 0 0 0 0 2.4 0 0 2.5 3.6 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 180.2293 226.7762 207.6726 210.7903 174.4542 200.4486 230.5451 300.7082 280.2147 259.5823 255.9507 245.1615 252.085 308.4401 274.5539 248.7656 266.2584 237.4361
Actin 0.0065 0.0002 0.0001 0.0001 0.0009 0.0001 0.006 0.0002 0.001 0.0001 0.0003 0.0002 0.0003 0.0024 0.0059 0.0006 0.0001 0.0003
Bud 0.0007 0 0.0001 0 0.0029 0 0.0008 0.0009 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0004 0.0009 0 0.0002
Bud Neck 0.0007 0.0001 0.0002 0.0002 0.0004 0.0004 0.0005 0.0001 0.0001 0.0002 0.0003 0.001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0003
Bud Periphery 0.0007 0 0 0 0.0041 0 0.0007 0.0002 0.0001 0 0 0 0 0.0001 0.0003 0.0008 0 0
Bud Site 0.0014 0 0 0 0.0004 0 0.0015 0.0001 0.0001 0.0001 0.0002 0 0 0 0.0004 0.0002 0 0
Cell Periphery 0.001 0.0001 0.0003 0 0.0005 0 0.0007 0.0001 0.0002 0.0006 0.0004 0.0008 0.0004 0 0.0003 0.0006 0 0.0001
Cytoplasm 0.8719 0.9902 0.9794 0.9769 0.8529 0.9889 0.9073 0.9836 0.972 0.9478 0.8774 0.9767 0.938 0.9899 0.9622 0.9578 0.9934 0.9685
Cytoplasmic Foci 0.0075 0.0001 0.0003 0.0001 0.0136 0.0001 0.0039 0.0036 0.0007 0.0082 0.0506 0.0007 0.0046 0.0001 0.0045 0.0008 0.0001 0.0002
Eisosomes 0.0005 0 0 0 0.0004 0 0.0009 0 0.0004 0.0007 0.001 0 0.0003 0 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0007 0 0.0012 0.0001 0.0002 0.0005 0.0003 0 0.0004 0.0003 0 0.0002
Endosome 0.0007 0 0 0 0.0003 0 0.005 0 0.0009 0.0001 0.0003 0 0.0003 0 0.0017 0.0001 0 0.0001
Golgi 0.0005 0 0 0 0.0001 0 0.001 0 0.0004 0 0.0004 0 0 0 0.0007 0 0 0
Lipid Particles 0.0034 0 0.0001 0 0.0023 0 0.0025 0.0001 0.0004 0.0018 0.0104 0.0002 0.0005 0 0.0029 0.0001 0 0
Mitochondria 0.0051 0.0001 0.0003 0.0001 0.0519 0 0.0052 0.0006 0.0014 0.0062 0.0062 0.0002 0.0013 0.0028 0.0039 0.0026 0 0.0004
None 0.0056 0.0002 0.0006 0.0001 0.003 0.0001 0.0056 0.0004 0.0016 0.0142 0.0187 0.0011 0.0032 0.0003 0.0022 0.0055 0.0002 0.0003
Nuclear Periphery 0.0084 0.0019 0.0024 0.0036 0.0066 0.0016 0.008 0.0007 0.0052 0.002 0.0021 0.0085 0.0088 0.0008 0.0012 0.005 0.0007 0.0061
Nucleolus 0.0176 0.0001 0.0025 0 0.0301 0 0.0109 0.0011 0.0001 0.0052 0.0172 0.0003 0.004 0 0.0037 0.0006 0 0.0003
Nucleus 0.0576 0.0067 0.0122 0.0177 0.0227 0.0084 0.0287 0.0077 0.0132 0.0085 0.0088 0.008 0.0359 0.0029 0.0059 0.0222 0.005 0.0217
Peroxisomes 0.0019 0 0 0 0.0021 0 0.0007 0 0 0.0005 0.0011 0 0.0001 0.0001 0.0004 0.0001 0 0
Punctate Nuclear 0.0014 0.0001 0.0001 0 0.0009 0.0001 0.0049 0.0001 0.0003 0.0003 0.002 0.0004 0.0005 0 0.0005 0.0002 0.0001 0.0002
Vacuole 0.0038 0.0001 0.0008 0.0007 0.0021 0.0001 0.0028 0.0003 0.0004 0.0017 0.001 0.0009 0.0007 0 0.0011 0.0011 0.0001 0.0006
Vacuole Periphery 0.0025 0 0.0003 0.0003 0.0018 0 0.0019 0.0002 0.0003 0.0015 0.0014 0.0002 0.0004 0.0001 0.0009 0.0004 0.0001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1439.4667 1573.8625 2237.9089 2099.6179 2058.7743 1778.278 1838.5523 1862.2026 2239.2927 1839.3815
Translational Efficiency 2.0467 2.0417 1.8024 2.1604 2.1139 1.9637 2.0668 2.491 2.0771 2.3203

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1084 905 111 1739 226 2441 2767 1151 1310 3346 2878 2890

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 12642.30 16676.63 22964.40 22289.99 15627.72 15052.91 19085.29 21712.15 13157.34 15492.08 19234.90 22059.85
Standard Deviation 2722.32 3636.94 3883.99 4818.14 3288.62 3181.55 3743.06 6050.08 3044.77 3388.56 3822.29 5350.41
Intensity Change Log 2 — 0.399569 0.861140 0.818137 — -0.054065 0.288354 0.474395 — 0.166554 0.572822 0.638301

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000583 0.000210 0.000678 0.000310 0.000225 0.000072 0.000355 0.000452 0.000521 0.000109 0.000368 0.000366
Bud Neck 0.004646 0.001001 0.012705 0.005057 0.003174 0.000371 0.001742 0.008282 0.004392 0.000541 0.002165 0.006341
Bud Site 0.031617 0.006496 0.039729 0.042368 0.003727 0.001745 0.014395 0.093375 0.026805 0.003030 0.015372 0.062683
Cell Periphery 0.012225 0.012341 0.027909 0.003380 0.039469 0.001139 0.001404 0.003080 0.016925 0.004169 0.002427 0.003260
Cytoplasm 0.610884* 0.829642* 0.263880* 0.754311* 0.584528* 0.914189* 0.884325* 0.658298* 0.606337* 0.891321* 0.860395* 0.716072*
Cytoplasmic Foci 0.151769 0.016416 0.294715* 0.056291 0.069720 0.007124 0.023082 0.106504 0.137614 0.009637 0.033558 0.076290
Eisosomes 0.000007 0.000002 0.000019 0.000004 0.000012 0.000000 0.000001 0.000006 0.000008 0.000001 0.000002 0.000004
Endoplasmic Reticulum 0.000199 0.000088 0.000062 0.000069 0.000081 0.000047 0.000020 0.000056 0.000179 0.000058 0.000021 0.000063
Endosome 0.001154 0.000220 0.000917 0.001623 0.000305 0.000254 0.000382 0.002890 0.001007 0.000245 0.000402 0.002127
Golgi 0.037963 0.013096 0.017851 0.002987 0.035019 0.003102 0.001121 0.005829 0.037455 0.005805 0.001767 0.004119
Lipid Particles 0.014558 0.010821 0.024611 0.005820 0.020579 0.001225 0.001939 0.003475 0.015597 0.003820 0.002813 0.004886
Mitochondria 0.000452 0.000142 0.000183 0.000236 0.000390 0.000131 0.000195 0.001311 0.000441 0.000134 0.000195 0.000664
Mitotic Spindle 0.007466 0.001012 0.013378 0.011443 0.000979 0.001716 0.002696 0.010548 0.006347 0.001525 0.003108 0.011087
None 0.006059 0.005828 0.001733 0.005007 0.004782 0.006823 0.002954 0.005211 0.005838 0.006554 0.002906 0.005088
Nuclear Periphery 0.000574 0.000735 0.002779 0.000228 0.001884 0.000129 0.000086 0.000218 0.000800 0.000292 0.000190 0.000224
Nuclear Periphery Foci 0.011073 0.006818 0.008309 0.002861 0.011565 0.001711 0.000891 0.001565 0.011158 0.003092 0.001178 0.002345
Nucleolus 0.000247 0.000068 0.000239 0.000338 0.000324 0.000069 0.000124 0.000465 0.000260 0.000069 0.000128 0.000388
Nucleus 0.035870 0.051631 0.088378 0.044132 0.081242 0.047019 0.035746 0.041824 0.043697 0.048266 0.037776 0.043213
Peroxisomes 0.000316 0.000088 0.000378 0.000979 0.000022 0.000019 0.000403 0.001354 0.000265 0.000038 0.000402 0.001128
Vacuole 0.045140 0.035571 0.186907 0.059286 0.114113 0.010089 0.026956 0.050491 0.057040 0.016981 0.033125 0.055783
Vacuole Periphery 0.027198 0.007773 0.014642 0.003271 0.027860 0.003027 0.001182 0.004768 0.027312 0.004311 0.001701 0.003867

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.08 0.22 2.24 -1.96 2.59 2.60 -0.73 -3.41 -6.60 -0.80 4.02 1.28 1.65 -6.73 -0.26
Bud Neck 8.46 -5.80 -3.94 -12.41 4.20 2.24 0.66 -5.60 -14.31 -11.41 9.91 3.80 -7.77 -19.88 -13.13
Bud Site 9.51 -0.76 -2.75 -13.03 -0.40 1.88 -8.66 -17.54 -18.40 -14.52 11.90 4.37 -9.90 -23.72 -15.89
Cell Periphery -0.66 -3.01 9.18 8.65 5.66 8.92 8.85 8.58 -2.97 -2.24 10.54 12.60 12.30 4.08 -0.56
Cytoplasm -16.71 13.30 -5.99 13.48 -17.05 -15.65 -12.85 -0.64 31.17 24.68 -28.87 -22.12 -4.22 36.41 25.04
Cytoplasmic Foci 17.38 -4.82 9.12 -12.93 7.81 6.89 4.02 -4.47 -18.81 -13.80 19.80 13.86 6.15 -23.88 -12.06
Eisosomes 3.17 -3.29 2.21 -3.77 5.33 3.40 3.12 1.40 -11.43 -9.73 5.01 4.35 2.15 -13.86 -9.84
Endoplasmic Reticulum 3.08 3.58 4.01 2.37 1.11 1.85 4.60 2.01 -0.35 -4.72 3.97 5.40 4.24 0.31 -5.23
Endosome 10.49 0.82 -1.78 -7.29 -2.16 0.33 -1.90 -5.15 -5.17 -4.71 8.18 5.68 -3.99 -8.10 -6.90
Golgi 8.93 7.18 14.18 8.63 6.55 8.74 9.33 8.19 -1.53 -4.07 14.48 16.73 15.68 4.41 -4.53
Lipid Particles 1.30 -2.97 3.21 1.99 4.37 7.37 6.86 5.52 -6.27 -4.40 7.64 8.04 5.24 -4.38 -5.25
Mitochondria 4.64 3.90 3.51 -2.00 -0.69 1.39 1.08 -1.00 -2.15 -1.94 5.14 3.91 0.02 -2.58 -2.08
Mitotic Spindle 5.66 -0.53 -5.34 -8.62 -1.25 -2.03 -5.82 -7.09 -6.38 -4.66 4.62 0.65 -6.75 -10.32 -7.47
None 0.25 4.68 1.81 2.30 -3.20 -2.67 3.00 0.58 3.00 -1.91 -0.77 3.48 1.92 3.70 -1.99
Nuclear Periphery -1.88 -5.29 2.19 2.90 5.55 9.35 9.40 7.65 -7.03 -7.37 8.02 9.98 6.38 -3.15 -5.94
Nuclear Periphery Foci 3.13 0.27 6.16 3.10 2.26 6.83 7.20 6.25 -1.95 -3.48 9.07 11.13 8.41 -0.76 -4.99
Nucleolus 5.60 0.68 -2.61 -6.21 -3.21 2.13 1.36 -2.34 -7.40 -6.19 5.78 2.97 -4.28 -9.49 -7.33
Nucleus -5.08 -4.06 -6.17 -0.40 2.64 4.51 5.87 3.97 -0.78 -3.75 -2.61 0.59 -3.67 -1.51 -5.12
Peroxisomes 2.37 -0.09 -3.37 -5.55 -3.22 0.62 -6.58 -4.93 -4.96 -2.81 3.45 -1.58 -4.86 -8.00 -3.89
Vacuole 1.43 -7.29 -8.09 -9.26 5.22 9.01 7.08 2.88 -15.94 -10.44 11.05 4.92 -5.78 -21.34 -13.21
Vacuole Periphery 6.96 4.43 9.58 4.70 3.96 4.59 4.98 4.39 -1.11 -4.19 9.76 11.24 10.38 1.92 -4.42
Standard name
Human Ortholog
Description Thioredoxin peroxidase; acts as both ribosome-associated and free cytoplasmic antioxidant; self-associates to form high-molecular weight chaperone complex under oxidative stress; chaperone activity essential for growth in zinc deficiency; required for telomere length maintenance; binds and modulates Cdc19p activity; protein abundance increases, forms cytoplasmic foci during DNA replication stress; TSA1 has a paralog, TSA2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Tsa1

Tsa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tsa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available