ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; has some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 17 | 17 | 9 | 9 | 0 | 4 | 1 | 0 | 1 | 6 | 0 | 28 | 38 | 2 | 12 | 11 | 30 | 40 | 25 | 15 | 27 | 45 | 60 |
Bud | 25 | 6 | 11 | 12 | 8 | 11 | 30 | 17 | 26 | 18 | 31 | 16 | 9 | 21 | 3 | 2 | 9 | 16 | 5 | 8 | 2 | 6 | 9 | 8 |
Bud Neck | 0 | 0 | 9 | 2 | 10 | 1 | 4 | 2 | 4 | 2 | 6 | 3 | 10 | 8 | 1 | 0 | 1 | 2 | 6 | 7 | 1 | 7 | 6 | 6 |
Bud Site | 0 | 1 | 0 | 3 | 0 | 1 | 7 | 6 | 11 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 4 | 2 | 1 | 1 | 1 | 2 | 2 | 0 | 3 | 4 | 0 | 1 | 0 | 9 | 7 | 4 | 0 | 0 | 0 | 0 | 1 | 2 |
Cytoplasm | 60 | 60 | 56 | 63 | 92 | 66 | 82 | 66 | 94 | 91 | 84 | 83 | 110 | 172 | 229 | 143 | 86 | 96 | 15 | 22 | 15 | 30 | 46 | 49 |
Endoplasmic Reticulum | 16 | 15 | 10 | 1 | 1 | 4 | 3 | 0 | 5 | 0 | 6 | 0 | 22 | 12 | 5 | 22 | 15 | 4 | 2 | 2 | 4 | 4 | 6 | 8 |
Endosome | 7 | 17 | 3 | 6 | 11 | 11 | 4 | 13 | 4 | 1 | 1 | 9 | 23 | 45 | 114 | 66 | 39 | 47 | 80 | 47 | 31 | 43 | 75 | 76 |
Golgi | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 2 | 23 | 17 | 4 | 12 | 17 | 25 |
Mitochondria | 169 | 42 | 11 | 25 | 15 | 87 | 167 | 117 | 272 | 144 | 286 | 141 | 10 | 7 | 2 | 16 | 16 | 24 | 106 | 83 | 100 | 118 | 196 | 176 |
Nucleus | 1 | 0 | 2 | 0 | 3 | 1 | 1 | 8 | 6 | 3 | 1 | 2 | 0 | 0 | 0 | 5 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 1 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2 | 3 | 1 | 9 | 3 | 4 | 4 | 6 | 7 | 8 | 3 | 7 | 5 | 4 | 42 | 12 | 4 | 0 | 21 | 4 | 10 | 12 | 18 | 22 |
SpindlePole | 5 | 3 | 27 | 25 | 23 | 18 | 24 | 29 | 42 | 33 | 20 | 43 | 38 | 44 | 62 | 58 | 26 | 22 | 9 | 4 | 11 | 17 | 29 | 24 |
Vac/Vac Membrane | 4 | 8 | 4 | 2 | 3 | 5 | 4 | 4 | 4 | 2 | 4 | 7 | 2 | 8 | 8 | 53 | 11 | 10 | 8 | 18 | 6 | 5 | 12 | 9 |
Unique Cell Count | 241 | 156 | 129 | 133 | 157 | 170 | 262 | 209 | 366 | 249 | 354 | 257 | 229 | 321 | 404 | 329 | 188 | 211 | 326 | 248 | 210 | 297 | 472 | 478 |
Labelled Cell Count | 306 | 176 | 155 | 160 | 179 | 210 | 337 | 272 | 478 | 311 | 456 | 315 | 257 | 361 | 470 | 401 | 226 | 262 | 326 | 248 | 210 | 297 | 472 | 478 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.7 | 5.4 | 5.3 | 5.0 | 4.4 | 3.9 | 4.6 | 4.0 | 3.9 | 3.6 | 4.0 | 5.3 | 5.7 | 5.7 | 7.2 | 6.6 | 6.3 | 5.7 | 5.7 | 6.3 |
Std Deviation (1e-4) | 0.5 | 0.8 | 1.2 | 2.0 | 1.4 | 1.7 | 1.4 | 1.4 | 1.5 | 1.1 | 0.9 | 1.1 | 0.9 | 0.9 | 1.3 | 1.2 | 1.2 | 1.2 | 1.5 | 1.1 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.11 | -0.31 | -0.46 | -0.25 | -0.45 | -0.49 | -0.59 | -0.45 | -0.02 | 0.06 | 0.07 | 0.42 | 0.29 | 0.22 | 0.07 | 0.06 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.7 | -2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.3 | -0.4 | 0 | -3.8 | -2.3 | 0.3 |
Bud | 0.1 | -1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.8 | -0.7 | 0 | 0 | -1.3 | -0.3 |
Bud Neck | -2.2 | -0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | 2.6 | -0.8 | -2.4 | -2.2 | -3.8 | -1.3 | -4.2 | -2.1 | 0.8 | 2.0 | 2.6 | 0 | 0.4 | 0.4 |
Endoplasmic Reticulum | -2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | -1.8 | 0 | -0.4 | 0.1 | -2.6 |
Endosome | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 3.6 | 6.2 | 4.8 | 4.8 | 5.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.4 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.6 | -3.1 | 0 | -1.5 | 0 | 0.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 | 0 |
SpindlePole | -0.4 | -1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | -1.9 | -1.5 | -0.8 | -1.7 | -2.7 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | 1.1 | 0.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0298 | 0.3322 | 0.2057 | -0.0993 | 0.1048 | 0.3111 | 0.4597 | 0.5163 | 0.5177 | 0.9396 | 0.6956 | 0.5621 | 0.18 | 0.4815 | 0.1782 | 0.7135 | 0.4192 | 0.3238 |
Actin | 0.0047 | 0.0038 | 0.0181 | 0.0017 | 0.0036 | 0.0037 | 0.0196 | 0.0079 | 0.0219 | 0.0498 | 0.006 | 0.0092 | 0.0453 | 0.0165 | 0.0238 | 0.0385 | 0.0235 | 0.0143 |
Bud | 0.0009 | 0.0066 | 0.0135 | 0.0011 | 0.0011 | 0.001 | 0.0068 | 0.0057 | 0.0067 | 0.0236 | 0.0019 | 0.003 | 0.0019 | 0.0067 | 0.0111 | 0.0008 | 0.0018 | 0.0018 |
Bud Neck | 0.0006 | 0.0009 | 0.003 | 0.0029 | 0.0012 | 0.0083 | 0.004 | 0.0014 | 0.0108 | 0.0135 | 0.0028 | 0.0294 | 0.0042 | 0.0014 | 0.0083 | 0.0017 | 0.002 | 0.0274 |
Bud Periphery | 0.0015 | 0.0048 | 0.0066 | 0.0015 | 0.0015 | 0.0011 | 0.0032 | 0.004 | 0.0137 | 0.0109 | 0.0019 | 0.0012 | 0.0028 | 0.0021 | 0.0115 | 0.0008 | 0.0029 | 0.0043 |
Bud Site | 0.0019 | 0.0218 | 0.0169 | 0.0009 | 0.0005 | 0.0018 | 0.0153 | 0.0371 | 0.0224 | 0.009 | 0.0321 | 0.0014 | 0.0099 | 0.0332 | 0.0191 | 0.0014 | 0.0025 | 0.0011 |
Cell Periphery | 0.0004 | 0.0007 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0009 | 0.0011 | 0.0005 | 0.0004 | 0.0002 | 0.0007 | 0.0006 | 0.0005 | 0.0002 | 0.0002 | 0.0002 |
Cytoplasm | 0.1173 | 0.1834 | 0.1178 | 0.1659 | 0.1284 | 0.1473 | 0.104 | 0.1822 | 0.146 | 0.1639 | 0.1397 | 0.1414 | 0.1519 | 0.2062 | 0.1875 | 0.1776 | 0.1722 | 0.1351 |
Cytoplasmic Foci | 0.1882 | 0.1919 | 0.2135 | 0.2132 | 0.1681 | 0.2431 | 0.253 | 0.2019 | 0.2182 | 0.1865 | 0.2909 | 0.3139 | 0.265 | 0.2468 | 0.2387 | 0.2055 | 0.2563 | 0.2786 |
Eisosomes | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0004 | 0.0009 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.001 | 0.0002 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0038 | 0.0049 | 0.0035 | 0.0065 | 0.002 | 0.0031 | 0.005 | 0.0027 | 0.0048 | 0.004 | 0.0045 | 0.002 | 0.0074 | 0.0038 | 0.0044 | 0.0051 | 0.0036 | 0.0051 |
Endosome | 0.2024 | 0.1563 | 0.1767 | 0.2717 | 0.135 | 0.167 | 0.1736 | 0.16 | 0.1622 | 0.1112 | 0.2635 | 0.1428 | 0.22 | 0.2249 | 0.2036 | 0.3418 | 0.2672 | 0.2768 |
Golgi | 0.0263 | 0.0207 | 0.0399 | 0.0329 | 0.018 | 0.0246 | 0.0296 | 0.0334 | 0.0315 | 0.0302 | 0.0253 | 0.0302 | 0.0452 | 0.0468 | 0.0503 | 0.0457 | 0.0559 | 0.0563 |
Lipid Particles | 0.0328 | 0.0128 | 0.016 | 0.023 | 0.0279 | 0.0252 | 0.0384 | 0.0061 | 0.0109 | 0.0088 | 0.0081 | 0.0096 | 0.0327 | 0.0168 | 0.0086 | 0.0079 | 0.0112 | 0.0107 |
Mitochondria | 0.0141 | 0.0151 | 0.0427 | 0.0459 | 0.0091 | 0.0212 | 0.0102 | 0.0101 | 0.0536 | 0.0145 | 0.0147 | 0.0178 | 0.01 | 0.0121 | 0.023 | 0.0129 | 0.0267 | 0.024 |
None | 0.3628 | 0.3265 | 0.2506 | 0.1594 | 0.4208 | 0.2732 | 0.2383 | 0.3063 | 0.2303 | 0.2901 | 0.1216 | 0.2016 | 0.1417 | 0.1275 | 0.1454 | 0.1061 | 0.0817 | 0.0952 |
Nuclear Periphery | 0.0018 | 0.0037 | 0.0013 | 0.0038 | 0.0025 | 0.0024 | 0.0087 | 0.0008 | 0.004 | 0.006 | 0.0145 | 0.0029 | 0.0076 | 0.0011 | 0.0092 | 0.0022 | 0.0173 | 0.0021 |
Nucleolus | 0.001 | 0.0014 | 0.0013 | 0.0006 | 0.001 | 0.0012 | 0.0015 | 0.0011 | 0.0021 | 0.0038 | 0.0029 | 0.0007 | 0.0009 | 0.0024 | 0.0015 | 0.0005 | 0.001 | 0.0008 |
Nucleus | 0.0021 | 0.0037 | 0.0021 | 0.0032 | 0.0019 | 0.0024 | 0.0035 | 0.0035 | 0.0032 | 0.0135 | 0.013 | 0.01 | 0.0041 | 0.0045 | 0.0029 | 0.0013 | 0.0029 | 0.0024 |
Peroxisomes | 0.0176 | 0.021 | 0.0619 | 0.0401 | 0.0528 | 0.0586 | 0.0556 | 0.0229 | 0.0384 | 0.0253 | 0.0323 | 0.0631 | 0.0308 | 0.0287 | 0.033 | 0.0302 | 0.0567 | 0.0489 |
Punctate Nuclear | 0.0052 | 0.0065 | 0.0047 | 0.003 | 0.0084 | 0.0058 | 0.0211 | 0.0029 | 0.0038 | 0.0227 | 0.008 | 0.0129 | 0.0053 | 0.0045 | 0.0046 | 0.0028 | 0.0043 | 0.0055 |
Vacuole | 0.0121 | 0.0113 | 0.0068 | 0.0166 | 0.0123 | 0.0066 | 0.0056 | 0.0078 | 0.0107 | 0.009 | 0.0112 | 0.0052 | 0.0095 | 0.0117 | 0.0095 | 0.0139 | 0.0051 | 0.0072 |
Vacuole Periphery | 0.0022 | 0.0021 | 0.002 | 0.0058 | 0.0034 | 0.002 | 0.0017 | 0.0012 | 0.0031 | 0.0024 | 0.0045 | 0.0016 | 0.0025 | 0.0016 | 0.0027 | 0.003 | 0.0045 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.8537 | 2.8926 | 5.7238 | 5.2812 | 2.7969 | 11.858 | 5.8494 | 4.277 | 6.5259 | 6.3078 |
Translational Efficiency | 1.2354 | 1.7093 | 0.8134 | 0.9886 | 1.874 | 0.639 | 1.0608 | 1.0015 | 0.9067 | 1.0103 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
333 | 1357 | 2270 | 971 | 1762 | 1220 | 1717 | 1659 | 2095 | 2577 | 3987 | 2630 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 706.03 | 747.96 | 821.51 | 815.17 | 673.62 | 759.57 | 864.67 | 705.79 | 678.77 | 753.46 | 840.10 | 746.17 |
Standard Deviation | 80.13 | 99.68 | 85.65 | 100.47 | 77.88 | 93.23 | 98.79 | 86.43 | 79.13 | 96.86 | 94.00 | 105.95 |
Intensity Change Log 2 | — | 0.083232 | 0.218549 | 0.207371 | — | 0.173248 | 0.360215 | 0.067304 | — | 0.127884 | 0.289456 | 0.140680 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001655 | 0.002231 | 0.009582 | 0.019676 | 0.001511 | 0.002308 | 0.006231 | 0.007630 | 0.001534 | 0.002267 | 0.008139 | 0.012077 |
Bud Neck | 0.034791 | 0.013814 | 0.046769 | 0.019604 | 0.016798 | 0.018080 | 0.018633 | 0.035672 | 0.019658 | 0.015834 | 0.034652 | 0.029740 |
Bud Site | 0.019027 | 0.032184 | 0.046363 | 0.062899 | 0.012470 | 0.024333 | 0.035358 | 0.037761 | 0.013512 | 0.028467 | 0.041624 | 0.047042 |
Cell Periphery | 0.000848 | 0.000171 | 0.000108 | 0.000297 | 0.000126 | 0.000131 | 0.000186 | 0.000147 | 0.000241 | 0.000152 | 0.000142 | 0.000202 |
Cytoplasm | 0.125381* | 0.108553* | 0.163156* | 0.186001* | 0.101249* | 0.126302* | 0.268367* | 0.140271* | 0.105085* | 0.116956* | 0.208465* | 0.157155* |
Cytoplasmic Foci | 0.431146* | 0.442494* | 0.237206* | 0.241754* | 0.462635* | 0.433148* | 0.225392* | 0.348362* | 0.457630* | 0.438069* | 0.232118* | 0.309002* |
Eisosomes | 0.000938 | 0.000252 | 0.000165 | 0.000281 | 0.000336 | 0.000273 | 0.000142 | 0.000230 | 0.000432 | 0.000262 | 0.000155 | 0.000249 |
Endoplasmic Reticulum | 0.001087 | 0.001754 | 0.002497 | 0.006406 | 0.001236 | 0.001142 | 0.003843 | 0.000829 | 0.001213 | 0.001464 | 0.003077 | 0.002888 |
Endosome | 0.061913 | 0.098027 | 0.056918 | 0.048985 | 0.068520 | 0.078112 | 0.041132 | 0.076744 | 0.067470 | 0.088599 | 0.050120 | 0.066495 |
Golgi | 0.125179* | 0.125335* | 0.017037 | 0.010589 | 0.135400* | 0.128680* | 0.008683 | 0.025122 | 0.133776* | 0.126918* | 0.013439 | 0.019756 |
Lipid Particles | 0.020966 | 0.014689 | 0.016923 | 0.018617 | 0.031847 | 0.017943 | 0.016486 | 0.020317 | 0.030118 | 0.016229 | 0.016735 | 0.019689 |
Mitochondria | 0.085727 | 0.056018 | 0.021026 | 0.004395 | 0.063109 | 0.060771 | 0.004600 | 0.010030 | 0.066704 | 0.058268 | 0.013952 | 0.007950 |
Mitotic Spindle | 0.004044 | 0.013145 | 0.097627 | 0.033529 | 0.009876 | 0.015922 | 0.072470 | 0.031009 | 0.008949 | 0.014459 | 0.086793 | 0.031939 |
None | 0.000410 | 0.001426 | 0.000430 | 0.000640 | 0.000940 | 0.000542 | 0.002000 | 0.000665 | 0.000856 | 0.001008 | 0.001106 | 0.000656 |
Nuclear Periphery | 0.000111 | 0.000138 | 0.001348 | 0.000609 | 0.000150 | 0.000115 | 0.001487 | 0.000302 | 0.000144 | 0.000127 | 0.001408 | 0.000416 |
Nuclear Periphery Foci | 0.000319 | 0.000625 | 0.001303 | 0.005196 | 0.000308 | 0.000194 | 0.004739 | 0.001283 | 0.000310 | 0.000421 | 0.002783 | 0.002728 |
Nucleolus | 0.000568 | 0.000770 | 0.001479 | 0.001005 | 0.000953 | 0.000402 | 0.002708 | 0.002072 | 0.000892 | 0.000596 | 0.002008 | 0.001678 |
Nucleus | 0.010171 | 0.005526 | 0.039194 | 0.052133 | 0.009859 | 0.006577 | 0.086458 | 0.024541 | 0.009909 | 0.006023 | 0.059548 | 0.034728 |
Peroxisomes | 0.053380 | 0.037704 | 0.135612* | 0.118271* | 0.060512 | 0.048116 | 0.082155 | 0.181348* | 0.059379 | 0.042633 | 0.112591* | 0.158060* |
Vacuole | 0.016373 | 0.036285 | 0.103957 | 0.168205 | 0.018289 | 0.031072 | 0.117984 | 0.054291 | 0.017985 | 0.033817 | 0.109998 | 0.096348 |
Vacuole Periphery | 0.005965 | 0.008860 | 0.001298 | 0.000910 | 0.003874 | 0.005837 | 0.000946 | 0.001372 | 0.004206 | 0.007429 | 0.001146 | 0.001202 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.07 | -9.17 | -9.69 | -9.61 | -5.02 | -2.20 | -6.48 | -5.65 | -4.64 | -1.48 | -2.84 | -12.60 | -10.87 | -9.86 | -3.63 |
Bud Neck | 4.27 | -2.43 | 3.34 | -2.76 | 12.66 | -0.69 | -1.26 | -6.99 | -6.19 | -6.34 | 2.40 | -8.62 | -4.44 | -7.42 | 3.58 |
Bud Site | -3.42 | -6.11 | -8.07 | -6.20 | -3.59 | -5.19 | -8.70 | -9.61 | -3.75 | -1.59 | -8.48 | -13.71 | -14.09 | -6.32 | -2.51 |
Cell Periphery | 2.18 | 2.40 | 1.84 | -2.92 | -10.23 | -0.21 | -3.80 | -1.13 | -0.62 | 2.26 | 1.68 | 2.13 | 0.97 | -1.94 | -5.72 |
Cytoplasm | 1.71 | -3.90 | -5.57 | -9.77 | -3.00 | -3.88 | -21.58 | -6.67 | -2.38 | 14.36 | -2.41 | -19.75 | -10.23 | -8.16 | 8.11 |
Cytoplasmic Foci | -0.92 | 16.80 | 16.38 | 26.79 | 1.06 | 3.86 | 38.32 | 15.57 | 10.24 | -20.03 | 3.27 | 44.36 | 25.29 | 22.80 | -15.93 |
Eisosomes | 4.32 | 4.93 | 4.27 | -0.43 | -6.90 | 2.17 | 9.36 | 4.77 | 2.15 | -6.67 | 5.03 | 9.03 | 6.06 | 1.51 | -8.85 |
Endoplasmic Reticulum | -2.03 | -6.24 | -10.61 | -8.66 | -7.29 | 0.32 | -11.13 | 2.17 | 1.08 | 14.36 | -1.15 | -11.19 | -7.68 | -5.72 | 1.86 |
Endosome | -5.76 | 1.41 | 3.33 | 12.97 | 3.52 | -2.46 | 10.83 | -1.85 | 0.63 | -11.70 | -6.63 | 8.06 | 1.42 | 8.20 | -6.66 |
Golgi | 0.02 | 12.09 | 12.78 | 26.32 | 4.89 | 1.02 | 30.68 | 25.47 | 21.33 | -10.44 | 1.37 | 32.04 | 29.69 | 32.57 | -5.67 |
Lipid Particles | 2.30 | 1.48 | 1.39 | -1.14 | 0.10 | 5.73 | 7.78 | 4.87 | -1.22 | -2.81 | 7.40 | 7.92 | 5.71 | -2.03 | -2.19 |
Mitochondria | 3.44 | 8.10 | 10.19 | 15.72 | 14.01 | 0.50 | 19.70 | 17.24 | 14.97 | -5.50 | 2.31 | 18.62 | 20.62 | 21.14 | 6.74 |
Mitotic Spindle | -2.51 | -18.24 | -5.73 | -4.64 | 13.03 | -2.51 | -14.79 | -8.09 | -4.90 | 8.01 | -3.29 | -25.16 | -10.01 | -7.02 | 15.40 |
None | -2.00 | -0.39 | -0.75 | 1.64 | -0.62 | 1.97 | -3.32 | 1.21 | -1.44 | 6.32 | -0.04 | -0.90 | 1.25 | 1.09 | 3.96 |
Nuclear Periphery | -0.82 | -10.06 | -11.17 | -10.74 | 6.20 | 1.66 | -10.91 | -5.52 | -6.57 | 9.65 | 0.82 | -14.59 | -11.10 | -11.57 | 11.54 |
Nuclear Periphery Foci | -1.40 | -4.83 | -7.87 | -7.25 | -6.03 | 3.06 | -10.96 | -7.86 | -8.90 | 7.94 | -1.14 | -12.41 | -10.38 | -9.33 | 0.63 |
Nucleolus | -0.78 | -4.67 | -1.75 | -0.39 | 3.82 | 3.06 | -5.52 | -3.74 | -6.07 | 2.15 | 1.54 | -6.12 | -3.47 | -4.94 | 2.79 |
Nucleus | 2.72 | -13.55 | -13.14 | -15.88 | -3.31 | 3.23 | -23.10 | -8.31 | -10.57 | 17.68 | 4.63 | -26.88 | -15.26 | -18.70 | 11.71 |
Peroxisomes | 2.81 | -10.63 | -6.15 | -10.67 | 3.51 | 3.26 | -1.87 | -18.41 | -19.99 | -16.10 | 5.87 | -11.22 | -18.42 | -23.25 | -9.31 |
Vacuole | -7.03 | -26.65 | -26.36 | -23.57 | -11.85 | -5.88 | -28.86 | -14.49 | -8.70 | 17.26 | -9.44 | -40.17 | -27.48 | -22.02 | 3.53 |
Vacuole Periphery | -2.84 | 6.58 | 7.17 | 11.27 | 3.36 | -4.24 | 12.77 | 8.76 | 10.22 | -2.45 | -6.73 | 13.59 | 12.16 | 14.42 | -0.51 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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