Standard name | |
---|---|
Human Ortholog | |
Description | Putative nucleotide sugar transporter; has similarity to Vrg4p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 3 | 2 | 6 | 0 | 1 | 2 | 3 | 0 | 0 | 1 | 5 | 53 | 51 | 33 | 3 | 4 | 1 | 0 | 1 | 3 | 4 | 9 | 5 |
Bud | 0 | 0 | 4 | 3 | 0 | 0 | 2 | 7 | 1 | 2 | 3 | 5 | 3 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 8 | 25 | 32 | 143 | 171 | 220 | 277 | 153 | 187 | 126 | 202 | 15 | 15 | 14 | 27 | 19 | 15 | 1 | 0 | 1 | 1 | 3 | 0 |
Endoplasmic Reticulum | 3 | 3 | 5 | 2 | 8 | 18 | 18 | 14 | 7 | 19 | 5 | 13 | 36 | 38 | 26 | 2 | 1 | 4 | 1 | 0 | 3 | 3 | 12 | 7 |
Endosome | 14 | 0 | 46 | 105 | 29 | 20 | 64 | 61 | 22 | 11 | 13 | 27 | 19 | 19 | 16 | 5 | 7 | 1 | 26 | 15 | 81 | 60 | 142 | 89 |
Golgi | 15 | 7 | 87 | 42 | 7 | 6 | 11 | 4 | 4 | 4 | 2 | 10 | 190 | 195 | 214 | 5 | 22 | 37 | 47 | 25 | 148 | 129 | 187 | 127 |
Mitochondria | 13 | 27 | 74 | 55 | 6 | 34 | 44 | 84 | 55 | 85 | 64 | 56 | 32 | 32 | 22 | 2 | 5 | 6 | 4 | 3 | 6 | 12 | 22 | 13 |
Nucleus | 0 | 0 | 1 | 0 | 2 | 1 | 3 | 9 | 1 | 4 | 2 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 3 | 2 | 0 | 0 | 1 | 10 | 5 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 |
Nucleolus | 1 | 0 | 4 | 7 | 2 | 2 | 6 | 9 | 1 | 1 | 0 | 1 | 1 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 0 |
SpindlePole | 0 | 1 | 3 | 2 | 0 | 1 | 2 | 5 | 2 | 1 | 2 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 77 | 27 | 134 | 148 | 291 | 219 | 337 | 437 | 129 | 171 | 109 | 138 | 43 | 46 | 74 | 55 | 67 | 84 | 69 | 39 | 70 | 43 | 158 | 218 |
Unique Cell Count | 112 | 59 | 285 | 295 | 401 | 376 | 584 | 767 | 304 | 402 | 279 | 385 | 276 | 279 | 302 | 84 | 99 | 97 | 155 | 87 | 320 | 263 | 547 | 468 |
Labelled Cell Count | 140 | 77 | 389 | 404 | 488 | 473 | 712 | 921 | 381 | 487 | 328 | 460 | 395 | 403 | 406 | 102 | 128 | 149 | 155 | 87 | 320 | 263 | 547 | 468 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.2 | 8.2 | 9.9 | 9.8 | 10.9 | 8.9 | 9.0 | 8.3 | 8.2 | 7.7 | 7.4 | 7.8 | 13.1 | 13.1 | 14.5 | 11.1 | 13.8 | 17.3 | 11.6 | 11.2 | 11.7 |
Std Deviation (1e-4) | 2.5 | 1.9 | 2.0 | 1.9 | 2.5 | 2.4 | 2.3 | 2.0 | 4.1 | 1.9 | 1.7 | 1.8 | 2.4 | 2.4 | 2.6 | 2.4 | 3.5 | 3.7 | 1.8 | 2.2 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.14 | -0.16 | -0.13 | -0.26 | -0.27 | -0.36 | -0.42 | -0.34 | 0.4 | 0.4 | 0.55 | 0.16 | 0.48 | 0.8 | 0.23 | 0.18 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.4 | 7.2 | 5.2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8 | 8.1 | 10.2 | 8.9 | 8.7 | 11.0 | 10.6 | 9.8 | 11.8 | -1.5 | -1.6 | -2.0 | 5.4 | 2.8 | 1.9 |
Endoplasmic Reticulum | 0 | 0.2 | 2.1 | 1.1 | 0.1 | 0.5 | 2.1 | 0 | 1.3 | 5.1 | 5.3 | 3.7 | 0 | 0 | 0 |
Endosome | 5.3 | -3.7 | -4.6 | -2.2 | -3.9 | -3.4 | -6.3 | -4.5 | -3.7 | -3.4 | -3.5 | -4.3 | -2.4 | -2.3 | -3.9 |
Golgi | -4.7 | -10.8 | -10.6 | -12.5 | -15.4 | -9.8 | -11.2 | -9.7 | -10.2 | 9.1 | 9.3 | 9.8 | -4.6 | -1.6 | 1.4 |
Mitochondria | -2.1 | -9.8 | -5.8 | -7.4 | -6.1 | -2.3 | -1.5 | -0.8 | -3.7 | -4.3 | -4.4 | -6.1 | -4.7 | -4.4 | -4.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.8 | 6.8 | 2.9 | 3.0 | 2.9 | -1.1 | -1.2 | -1.9 | -2.9 | -8.0 | -7.8 | -5.7 | 3.0 | 3.5 | 6.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.0571 | 11.5288 | 10.7771 | 11.0711 | 10.2049 | 11.1554 | 8.853 | 13.0089 | 11.5079 | 10.939 | 10.172 | 11.4578 | 6.8252 | 12.3088 | 12.1337 | 12.8386 | 13.0701 | 12.4833 |
Actin | 0.0563 | 0.0004 | 0.0191 | 0.0004 | 0.0055 | 0.0002 | 0.017 | 0.0002 | 0.0074 | 0.0002 | 0.0029 | 0.004 | 0.0783 | 0.0257 | 0.0103 | 0.0018 | 0.0021 | 0.0251 |
Bud | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0031 | 0.0003 | 0.0004 | 0.0005 | 0.0006 | 0.0002 |
Bud Neck | 0.0043 | 0.0003 | 0.0008 | 0.0002 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0041 | 0.0005 | 0.0007 | 0.0006 | 0.0006 | 0.0004 |
Bud Periphery | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0064 | 0.0002 | 0.0004 | 0.0008 | 0.001 | 0.0004 |
Bud Site | 0.0028 | 0.0006 | 0.0021 | 0.0001 | 0.0011 | 0.0001 | 0.0005 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0058 | 0.0006 | 0.0015 | 0.0008 | 0.0005 | 0.0002 |
Cell Periphery | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0016 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0003 |
Cytoplasm | 0.0082 | 0.0122 | 0.0041 | 0.0045 | 0.0034 | 0.0014 | 0.0039 | 0.002 | 0.0031 | 0.0006 | 0.0008 | 0.0009 | 0.0232 | 0.0023 | 0.0055 | 0.001 | 0.0007 | 0.0013 |
Cytoplasmic Foci | 0.031 | 0.03 | 0.0339 | 0.0314 | 0.0227 | 0.0168 | 0.0379 | 0.0389 | 0.0361 | 0.0252 | 0.0285 | 0.0223 | 0.0557 | 0.0673 | 0.0485 | 0.0182 | 0.0138 | 0.0144 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0071 | 0.0029 | 0.0037 | 0.0149 | 0.0062 | 0.0019 | 0.0072 | 0.0009 | 0.0039 | 0.0003 | 0.0009 | 0.0011 | 0.022 | 0.001 | 0.0017 | 0.0012 | 0.0012 | 0.0035 |
Endosome | 0.3743 | 0.4926 | 0.38 | 0.6083 | 0.5558 | 0.4266 | 0.3196 | 0.3664 | 0.2845 | 0.3189 | 0.4199 | 0.3053 | 0.1524 | 0.3925 | 0.2747 | 0.4411 | 0.3681 | 0.2779 |
Golgi | 0.3152 | 0.31 | 0.4247 | 0.2527 | 0.2713 | 0.4198 | 0.4709 | 0.5554 | 0.6136 | 0.5536 | 0.4507 | 0.6069 | 0.1718 | 0.4547 | 0.5456 | 0.4195 | 0.5246 | 0.5065 |
Lipid Particles | 0.0213 | 0.0093 | 0.0127 | 0.0028 | 0.0048 | 0.0042 | 0.0224 | 0.0033 | 0.0095 | 0.0017 | 0.0155 | 0.0053 | 0.1407 | 0.0156 | 0.0127 | 0.0165 | 0.011 | 0.0399 |
Mitochondria | 0.0268 | 0.0263 | 0.0362 | 0.0049 | 0.0068 | 0.0076 | 0.0442 | 0.0117 | 0.0181 | 0.0495 | 0.0254 | 0.0148 | 0.04 | 0.007 | 0.0677 | 0.0213 | 0.0268 | 0.0347 |
None | 0.0067 | 0.0001 | 0.002 | 0.0001 | 0.0025 | 0 | 0.0081 | 0 | 0.0001 | 0 | 0 | 0 | 0.0181 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0266 | 0.0027 | 0.002 | 0.0071 | 0.0207 | 0.0012 | 0.0144 | 0.0003 | 0.0037 | 0.0002 | 0.0005 | 0.0005 | 0.0995 | 0.0007 | 0.0006 | 0.0011 | 0.0009 | 0.006 |
Nucleolus | 0.0024 | 0.0003 | 0.0003 | 0.0001 | 0.0014 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0226 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Nucleus | 0.0094 | 0.0006 | 0.0002 | 0.0005 | 0.0071 | 0.0001 | 0.002 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.032 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0007 |
Peroxisomes | 0.0088 | 0.0005 | 0.0058 | 0.0002 | 0.0007 | 0.0001 | 0.01 | 0.0003 | 0.001 | 0.0003 | 0.001 | 0.0005 | 0.0139 | 0.0026 | 0.0041 | 0.001 | 0.0006 | 0.0008 |
Punctate Nuclear | 0.0058 | 0.0001 | 0.0006 | 0.0001 | 0.0107 | 0 | 0.0033 | 0 | 0.0001 | 0 | 0 | 0 | 0.0516 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0003 |
Vacuole | 0.0362 | 0.0613 | 0.0253 | 0.0354 | 0.0318 | 0.0528 | 0.0147 | 0.0053 | 0.0059 | 0.0184 | 0.0181 | 0.0118 | 0.0374 | 0.0115 | 0.0078 | 0.0297 | 0.0173 | 0.0304 |
Vacuole Periphery | 0.0553 | 0.0494 | 0.0457 | 0.036 | 0.0462 | 0.0665 | 0.0227 | 0.0151 | 0.0116 | 0.0307 | 0.0353 | 0.026 | 0.0192 | 0.0171 | 0.0161 | 0.0437 | 0.0295 | 0.0569 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 61.4386 | 33.3987 | 36.8996 | 40.4655 | 44.673 | 43.1492 | 41.2738 | 46.0907 | 40.8473 | 63.4738 |
Translational Efficiency | 0.4772 | 0.5083 | 0.4642 | 0.4754 | 0.4274 | 0.7434 | 0.5157 | 0.4262 | 0.5963 | 0.4954 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
112 | 998 | 174 | 785 | 1957 | 1490 | 1253 | 970 | 2069 | 2488 | 1427 | 1755 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1110.23 | 1192.94 | 1507.88 | 1164.16 | 1291.61 | 1085.40 | 1258.87 | 1148.25 | 1281.79 | 1128.54 | 1289.23 | 1155.37 |
Standard Deviation | 144.19 | 234.20 | 223.03 | 207.47 | 266.34 | 179.22 | 176.97 | 179.80 | 264.40 | 209.80 | 200.51 | 192.83 |
Intensity Change Log 2 | — | 0.103663 | 0.441663 | 0.068431 | — | -0.250944 | -0.037041 | -0.169734 | — | -0.076157 | 0.204052 | -0.054743 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.015916 | 0.004247 | 0.002204 | 0.021568 | 0.001730 | 0.001319 | 0.007600 | 0.009835 | 0.002498 | 0.002493 | 0.006942 | 0.015083 |
Bud Neck | 0.001512 | 0.000475 | 0.001605 | 0.002934 | 0.000766 | 0.000168 | 0.000286 | 0.001088 | 0.000806 | 0.000291 | 0.000447 | 0.001913 |
Bud Site | 0.014919 | 0.003918 | 0.003832 | 0.038493 | 0.005018 | 0.002732 | 0.003688 | 0.018945 | 0.005554 | 0.003208 | 0.003706 | 0.027689 |
Cell Periphery | 0.000107 | 0.000298 | 0.000207 | 0.000472 | 0.003942 | 0.000150 | 0.000074 | 0.000155 | 0.003735 | 0.000209 | 0.000090 | 0.000297 |
Cytoplasm | 0.005807 | 0.001157 | 0.000942 | 0.016900 | 0.000575 | 0.000754 | 0.001035 | 0.016889 | 0.000858 | 0.000916 | 0.001023 | 0.016894 |
Cytoplasmic Foci | 0.204506* | 0.109180 | 0.072282 | 0.195514* | 0.032218 | 0.096375 | 0.142178 | 0.200208* | 0.041545 | 0.101512 | 0.133655 | 0.198108* |
Eisosomes | 0.000042 | 0.000049 | 0.000042 | 0.000155 | 0.000079 | 0.000033 | 0.000049 | 0.000063 | 0.000077 | 0.000039 | 0.000048 | 0.000104 |
Endoplasmic Reticulum | 0.001859 | 0.002303 | 0.004386 | 0.005492 | 0.009603 | 0.001196 | 0.001519 | 0.002097 | 0.009183 | 0.001640 | 0.001869 | 0.003615 |
Endosome | 0.387464* | 0.193819 | 0.154654 | 0.246131* | 0.104379 | 0.216860* | 0.159573 | 0.281988* | 0.119703 | 0.207617* | 0.158973 | 0.265949* |
Golgi | 0.247746* | 0.538786* | 0.604726* | 0.351926* | 0.416936* | 0.518016* | 0.637023* | 0.369809* | 0.407777* | 0.526347* | 0.633085* | 0.361810* |
Lipid Particles | 0.003593 | 0.011106 | 0.025022 | 0.014699 | 0.007295 | 0.001987 | 0.009048 | 0.010102 | 0.007095 | 0.005645 | 0.010996 | 0.012158 |
Mitochondria | 0.006478 | 0.006126 | 0.000972 | 0.005003 | 0.032943 | 0.018160 | 0.000725 | 0.003128 | 0.031510 | 0.013333 | 0.000755 | 0.003967 |
Mitotic Spindle | 0.002640 | 0.000599 | 0.009570 | 0.017297 | 0.000305 | 0.000772 | 0.002993 | 0.020878 | 0.000431 | 0.000702 | 0.003795 | 0.019276 |
None | 0.000022 | 0.000044 | 0.000023 | 0.000028 | 0.000300 | 0.000046 | 0.000011 | 0.000042 | 0.000285 | 0.000045 | 0.000012 | 0.000036 |
Nuclear Periphery | 0.000227 | 0.000153 | 0.001414 | 0.000147 | 0.000269 | 0.000102 | 0.000173 | 0.000198 | 0.000266 | 0.000122 | 0.000324 | 0.000176 |
Nuclear Periphery Foci | 0.002630 | 0.001457 | 0.005758 | 0.002762 | 0.001142 | 0.000304 | 0.001176 | 0.003581 | 0.001222 | 0.000767 | 0.001734 | 0.003215 |
Nucleolus | 0.001455 | 0.000051 | 0.000011 | 0.000026 | 0.000357 | 0.000032 | 0.000003 | 0.000098 | 0.000417 | 0.000039 | 0.000004 | 0.000066 |
Nucleus | 0.000149 | 0.000066 | 0.000025 | 0.000154 | 0.000551 | 0.000346 | 0.000009 | 0.000250 | 0.000529 | 0.000234 | 0.000011 | 0.000207 |
Peroxisomes | 0.015881 | 0.000974 | 0.000542 | 0.009573 | 0.000291 | 0.000975 | 0.000945 | 0.005610 | 0.001135 | 0.000975 | 0.000896 | 0.007383 |
Vacuole | 0.071961 | 0.078820 | 0.083579 | 0.061371 | 0.199110 | 0.078694 | 0.023927 | 0.046361 | 0.192227 | 0.078745 | 0.031201 | 0.053075 |
Vacuole Periphery | 0.015088 | 0.046372 | 0.028205 | 0.009356 | 0.182192* | 0.060980 | 0.007967 | 0.008675 | 0.173146* | 0.055120 | 0.010435 | 0.008979 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.36 | 1.62 | -0.67 | -5.68 | -6.71 | 0.83 | -4.50 | -4.40 | -4.68 | -1.02 | -0.01 | -3.59 | -7.30 | -7.46 | -4.17 |
Bud Neck | 2.44 | 0.07 | -2.34 | -6.01 | -2.92 | 9.18 | 6.79 | -2.32 | -5.78 | -5.04 | 6.96 | 4.51 | -5.52 | -8.00 | -7.23 |
Bud Site | 1.92 | 1.90 | -3.66 | -9.43 | -9.05 | 4.23 | 1.24 | -6.62 | -7.61 | -6.81 | 3.95 | 1.91 | -10.86 | -12.08 | -11.31 |
Cell Periphery | -3.70 | -2.08 | -3.83 | -1.76 | -2.75 | 9.90 | 10.11 | 9.88 | -0.25 | -3.54 | 9.71 | 10.05 | 9.43 | -1.92 | -4.90 |
Cytoplasm | 2.23 | 2.29 | -4.27 | -6.90 | -6.88 | -1.49 | -1.96 | -6.88 | -6.82 | -6.68 | -0.50 | -0.79 | -9.70 | -9.65 | -9.56 |
Cytoplasmic Foci | 5.71 | 7.48 | 0.62 | -12.16 | -13.31 | -22.05 | -28.37 | -31.47 | -17.75 | -8.99 | -21.67 | -24.34 | -36.84 | -21.44 | -12.24 |
Eisosomes | -0.54 | 0.08 | -3.02 | -2.87 | -3.08 | 8.99 | 4.94 | 1.92 | -4.36 | -1.87 | 6.88 | 5.11 | -1.59 | -3.88 | -3.33 |
Endoplasmic Reticulum | -0.33 | -1.47 | -1.86 | -2.12 | -0.58 | 10.50 | 9.80 | 8.53 | -2.10 | -1.23 | 9.85 | 9.24 | 5.53 | -2.80 | -2.37 |
Endosome | 7.84 | 8.69 | 5.85 | -5.61 | -6.45 | -21.15 | -11.51 | -23.03 | -7.41 | -14.81 | -18.42 | -7.98 | -24.33 | -9.09 | -16.83 |
Golgi | -10.86 | -10.36 | -3.51 | 14.73 | 10.32 | -9.87 | -21.54 | 4.44 | 13.28 | 23.57* | -12.97 | -22.72 | 5.40 | 19.71 | 29.24* |
Lipid Particles | -3.38 | -4.28 | -4.90 | -1.50 | 1.92 | 6.24 | -1.31 | -1.82 | -5.40 | -0.64 | 1.44 | -2.90 | -3.88 | -4.76 | -0.84 |
Mitochondria | 0.07 | 1.37 | 0.37 | 0.72 | -2.38 | 5.56 | 15.67 | 13.17 | 7.76 | -2.46 | 8.16 | 15.69 | 12.84 | 6.82 | -3.48 |
Mitotic Spindle | 2.18 | -1.25 | -5.37 | -7.28 | -1.58 | -2.14 | -3.86 | -6.19 | -6.04 | -5.24 | -1.40 | -3.95 | -8.97 | -8.85 | -6.85 |
None | -0.19 | 0.63 | 0.24 | 1.27 | -1.50 | 12.01 | 14.50 | 9.93 | -0.07 | -2.30 | 12.28 | 14.43 | 11.65 | 0.42 | -3.13 |
Nuclear Periphery | 0.89 | -3.63 | 0.82 | -0.26 | 4.37 | 4.53 | 2.92 | 0.87 | -1.29 | -0.39 | 4.81 | -1.18 | 1.77 | -1.34 | 2.37 |
Nuclear Periphery Foci | 1.05 | -0.61 | 0.35 | -1.91 | 1.67 | 6.27 | -0.19 | -2.89 | -3.89 | -2.83 | 2.29 | -1.59 | -3.41 | -4.31 | -2.66 |
Nucleolus | 1.02 | 1.03 | 1.02 | 0.77 | -3.30 | 1.97 | 2.15 | 1.46 | -1.57 | -2.24 | 1.98 | 2.13 | 1.84 | -1.20 | -2.70 |
Nucleus | 0.96 | 1.12 | -0.11 | -2.28 | -2.66 | 0.48 | 1.77 | 0.47 | -0.18 | -4.27 | 0.89 | 1.82 | 0.53 | -0.74 | -4.93 |
Peroxisomes | 4.24 | 4.36 | 1.72 | -6.84 | -7.18 | -4.62 | -4.44 | -5.95 | -5.18 | -5.21 | 0.70 | 1.01 | -8.10 | -8.52 | -8.59 |
Vacuole | -0.49 | -0.69 | -0.00 | 0.98 | 1.00 | 17.96 | 30.61* | 22.64 | 5.23 | -6.30 | 18.67 | 28.50 | 21.67 | 4.25 | -7.41 |
Vacuole Periphery | -5.74 | -2.07 | 1.21 | 12.20 | 4.22 | 23.40* | 39.34* | 38.95* | 18.25 | -0.61 | 25.14 | 37.75 | 38.37 | 21.88 | 1.58 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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