Standard name
Human Ortholog
Description U1 snRNP protein involved in splicing; contains multiple tetriatricopeptide repeats
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0.06 0 0.08 0.09 0 0 0 0 0.09 0.14 0.17 0 0.1 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0 0 0.05 0 0.08 0.08 0.06 0.25 0.14 0.49 0.24 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Nucleus 0.95 0.94 0.9 1.0 0.94 0.89 0.86 0.87 0.87 0.87 0.78 0.87 0.9 0.83 0.81 0.86 0.8 0.77 0.87 0.89 0.78 0.84 0.84 0.76
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0.05 0.05 0.1 0 0 0 0 0.14 0.07 0.1 0.14 0 0 0 0 0 0 0.07 0 0.09 0 0.06 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.08 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 0 1 1 2 0 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 1 0 0 0 1 2 0 5 3 0 1 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 8 1 0 10 4 14 16 1 3 1 4 20 53 65 7 18 17 0 2 0 1 1 5
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 0 0 0 0 0 2 1 0 6 10 0 0 0 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 2 9 1 0 1 3 1 7
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 1 1 1
Mitochondria 26 11 0 1 0 10 14 11 28 23 33 22 0 3 1 13 9 10 0 1 0 4 3 4
Nucleus 145 255 19 21 144 109 143 155 98 142 53 81 194 303 317 179 151 155 137 253 44 163 275 276
Nuclear Periphery 0 2 0 0 0 1 2 0 0 1 0 1 0 0 3 0 0 0 0 0 0 0 0 0
Nucleolus 22 14 1 2 1 4 4 6 16 12 7 13 2 8 6 9 3 4 11 11 5 8 19 29
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 2 2 1 7 0 4 0 0 2 5 4 1 2 4 0 2 0 0 9 5
Vac/Vac Membrane 0 0 1 0 1 1 2 3 0 1 0 0 3 1 2 14 12 16 3 7 2 7 11 20
Unique Cell Count 153 272 21 21 154 122 167 179 113 163 68 93 216 366 389 208 189 202 157 286 57 195 327 364
Labelled Cell Count 195 292 22 24 158 134 183 198 149 190 94 122 221 378 400 228 205 226 157 286 57 195 327 364


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 6.9 6.6 5.6 7.5 6.0 6.0 6.3 4.6 5.0 4.5 4.7 7.8 7.2 6.9 7.9 8.1 7.4 7.0 6.9 7.0
Std Deviation (1e-4) 0.8 1.3 1.2 0.6 1.8 1.7 1.6 1.4 1.1 0.8 0.9 1.3 1.4 1.6 1.4 2.1 2.3 1.8 1.3 1.3 1.4
Intensity Change (Log2) – – – -0.24 0.17 -0.15 -0.15 -0.07 -0.53 -0.42 -0.57 -0.49 0.22 0.11 0.05 0.25 0.28 0.15 0.08 0.06 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 1.4 0.5 -0.2 -0.6 -0.5 -0.5 -0.4 -1.3 -0.4 -0.1 -0.9 -1.0 -0.6 -1.2 -1.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0913 1.3047 1.0463 1.0556 0.7322 1.0835 1.7097 2.6691 1.7725 1.972 1.4655 1.8636 1.5655 1.6141 1.4265 1.3494 0.8044 1.1659
Actin 0.0173 0 0.0004 0 0.0313 0.0018 0.0006 0 0.0141 0.0094 0.0001 0.0022 0.0273 0 0.0109 0.0046 0.0023 0.0003
Bud 0.0013 0.0059 0.0009 0 0.0013 0.0001 0.0003 0.0001 0.0002 0.0034 0.0001 0.0001 0.0005 0 0.0004 0.0003 0.0001 0.0001
Bud Neck 0.0062 0.0001 0.0004 0 0.001 0.0014 0.0007 0.0002 0.0006 0.0016 0.0004 0.0006 0.0053 0 0.0006 0.0015 0.0006 0.0008
Bud Periphery 0.0028 0.0012 0.001 0 0.0044 0.0001 0.0009 0.0002 0.0002 0.0041 0.0004 0.0003 0.0012 0 0.0008 0.0004 0.0002 0.0003
Bud Site 0.0125 0.0026 0.0012 0 0.0026 0.0002 0.0005 0.0001 0.0011 0.0132 0.0002 0.0002 0.0042 0 0.0025 0.0081 0.0003 0.0002
Cell Periphery 0.0007 0.0001 0.0003 0 0.0011 0 0.0001 0 0.0001 0.0003 0.0001 0.0001 0.0003 0 0.0002 0.0003 0.0001 0.0001
Cytoplasm 0.0041 0.0009 0.0022 0 0.0005 0.0002 0.0004 0.0001 0.0029 0.005 0.0001 0.0002 0.0032 0 0.0011 0.0046 0.0002 0.0002
Cytoplasmic Foci 0.0153 0 0.0077 0 0.0024 0.0013 0.015 0 0.0015 0.0087 0.0112 0.0029 0.0114 0 0.0046 0.0065 0.0082 0.0013
Eisosomes 0.0002 0 0 0 0.0002 0 0 0 0.0002 0.0001 0 0 0.0003 0 0.0004 0.0008 0 0
Endoplasmic Reticulum 0.0018 0.0001 0.0004 0 0.0009 0.0008 0.0003 0 0.0017 0.0007 0.0001 0.0002 0.0016 0 0.0013 0.0023 0.0002 0.0003
Endosome 0.0165 0.0001 0.0057 0 0.0103 0.0043 0.0087 0.0001 0.007 0.028 0.0126 0.0045 0.0139 0 0.0061 0.0158 0.0117 0.0029
Golgi 0.0064 0 0.0019 0 0.0199 0.0019 0.003 0 0.0023 0.0223 0.0043 0.0044 0.006 0 0.0022 0.0051 0.0091 0.001
Lipid Particles 0.0203 0 0.0085 0 0.0175 0.0084 0.0058 0.0001 0.0008 0.0059 0.0241 0.0041 0.0202 0 0.009 0.0113 0.0434 0.0078
Mitochondria 0.0055 0.0002 0.0108 0 0.034 0.0061 0.0019 0.0007 0.0069 0.0102 0.0043 0.01 0.0066 0.0001 0.009 0.0019 0.0152 0.0013
None 0.0031 0.0001 0.0069 0 0.0005 0.0001 0.0002 0.0003 0.0005 0.0006 0.0001 0.001 0.0025 0 0.0033 0.0173 0.0002 0.0001
Nuclear Periphery 0.0177 0.0118 0.0033 0.0005 0.0048 0.0042 0.0014 0.0007 0.0048 0.0015 0.0029 0.0055 0.0127 0.0003 0.0055 0.0064 0.0019 0.0014
Nucleolus 0.0487 0.0136 0.022 0.011 0.1022 0.0649 0.0226 0.0276 0.0115 0.022 0.0565 0.0399 0.0295 0.0095 0.0127 0.0133 0.0663 0.0364
Nucleus 0.7804 0.9596 0.913 0.9881 0.7379 0.898 0.9211 0.9656 0.9374 0.8576 0.8759 0.9088 0.8187 0.9896 0.9202 0.8804 0.8087 0.9407
Peroxisomes 0.0104 0 0.0022 0 0.0034 0.002 0.0139 0 0.0007 0.0021 0.0046 0.0019 0.0097 0 0.0019 0.0046 0.0099 0.0016
Punctate Nuclear 0.0228 0.0032 0.0081 0.0003 0.0057 0.003 0.001 0.0039 0.0011 0.0014 0.0008 0.0117 0.0214 0.0003 0.0048 0.0119 0.0193 0.0012
Vacuole 0.0047 0.0002 0.0023 0 0.0146 0.0007 0.0011 0.0002 0.0023 0.0014 0.0007 0.0006 0.0022 0 0.0016 0.0023 0.001 0.0018
Vacuole Periphery 0.0016 0.0002 0.0008 0 0.0037 0.0004 0.0004 0.0001 0.0019 0.0007 0.0007 0.0007 0.0013 0 0.001 0.0005 0.0011 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.0982 13.0473 18.9761 20.8752 20.8158 13.4202 14.5113 16.2049 14.222 21.851
Translational Efficiency 0.5306 0.6034 0.5669 0.4925 0.5804 0.6179 0.6347 0.5468 0.7057 0.5577

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
323 1363 343 1499 2057 1503 589 128 2380 2866 932 1627

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 621.46 758.69 1172.40 967.24 828.62 866.42 1084.55 1131.83 800.51 815.19 1116.88 980.19
Standard Deviation 83.40 154.84 158.02 181.72 110.69 124.60 146.51 184.27 128.72 149.80 156.68 187.24
Intensity Change Log 2 — 0.287849 0.915731 0.638212 — 0.064356 0.388314 0.449875 — 0.164405 0.638242 0.533618

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000307 0.001059 0.001669 0.000932 0.000246 0.000421 0.001350 0.001388 0.000255 0.000724 0.001467 0.000968
Bud Neck 0.001390 0.007328 0.017800 0.022289 0.005896 0.020034 0.015416 0.019425 0.005285 0.013991 0.016293 0.022064
Bud Site 0.000623 0.002129 0.001939 0.006320 0.000505 0.001709 0.001973 0.004516 0.000521 0.001909 0.001960 0.006178
Cell Periphery 0.000133 0.000243 0.000086 0.000395 0.000052 0.000067 0.000114 0.000267 0.000063 0.000151 0.000104 0.000385
Cytoplasm 0.011931 0.008937 0.000303 0.032803 0.001375 0.002456 0.003584 0.008831 0.002807 0.005538 0.002376 0.030917
Cytoplasmic Foci 0.002299 0.000824 0.000042 0.004701 0.000182 0.000664 0.000046 0.000188 0.000469 0.000740 0.000044 0.004346
Eisosomes 0.000024 0.000030 0.000065 0.000050 0.000018 0.000023 0.000049 0.000033 0.000019 0.000026 0.000055 0.000049
Endoplasmic Reticulum 0.001579 0.005374 0.003302 0.003762 0.002667 0.004255 0.005176 0.005158 0.002519 0.004788 0.004486 0.003872
Endosome 0.000213 0.000535 0.000141 0.004627 0.000226 0.000986 0.000254 0.000597 0.000224 0.000772 0.000212 0.004310
Golgi 0.000111 0.000837 0.000141 0.002881 0.000188 0.000510 0.000125 0.000141 0.000177 0.000665 0.000131 0.002666
Lipid Particles 0.000471 0.000445 0.000096 0.001799 0.000134 0.000182 0.000161 0.000182 0.000180 0.000307 0.000137 0.001672
Mitochondria 0.001031 0.002264 0.002113 0.005105 0.000574 0.004078 0.001403 0.002307 0.000636 0.003215 0.001664 0.004885
Mitotic Spindle 0.000053 0.003621 0.000336 0.012676 0.000892 0.007799 0.001440 0.017526 0.000778 0.005812 0.001034 0.013057
None 0.032475 0.020578 0.001048 0.018336 0.002466 0.002381 0.006574 0.010581 0.006539 0.011035 0.004541 0.017726
Nuclear Periphery 0.000231 0.000515 0.000252 0.001290 0.000318 0.002203 0.000474 0.001056 0.000306 0.001400 0.000392 0.001272
Nuclear Periphery Foci 0.000322 0.001093 0.000052 0.000592 0.000218 0.000768 0.000159 0.000307 0.000232 0.000923 0.000119 0.000569
Nucleolus 0.043373 0.045298 0.029344 0.008362 0.032525 0.025075 0.020757 0.006748 0.033998 0.034693 0.023917 0.008235
Nucleus 0.902297* 0.895535* 0.938156* 0.860633* 0.950814* 0.921683* 0.938313* 0.909382* 0.944229* 0.909247* 0.938255* 0.864468*
Peroxisomes 0.000746 0.001589 0.001606 0.002371 0.000239 0.000362 0.000507 0.000319 0.000307 0.000946 0.000912 0.002210
Vacuole 0.000275 0.001323 0.001299 0.007292 0.000268 0.002813 0.001987 0.010752 0.000269 0.002104 0.001734 0.007564
Vacuole Periphery 0.000118 0.000443 0.000210 0.002783 0.000196 0.001531 0.000140 0.000293 0.000186 0.001013 0.000166 0.002587

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.82 -10.30 -5.84 -0.05 5.51 -6.02 -14.90 -8.57 -7.38 -0.52 -5.59 -19.97 -10.68 -3.36 5.13
Bud Neck -10.06 -12.42 -19.67 -14.73 -4.10 -10.32 -9.17 -9.16 -2.63 -2.44 -10.44 -12.99 -17.60 -10.41 -5.34
Bud Site -2.75 -6.50 -5.93 -3.52 -3.82 -4.71 -8.82 -6.65 -4.26 -3.08 -4.95 -11.36 -6.62 -4.39 -3.84
Cell Periphery -2.25 1.79 -3.71 -0.42 -6.89 -1.56 -4.55 -3.90 -3.40 -2.39 -4.34 -2.96 -9.51 -3.84 -7.62
Cytoplasm 1.00 3.89 -2.57 -5.22 -10.76 -2.41 -1.70 -3.33 -2.30 -2.20 -3.14 1.61 -9.23 -7.48 -9.86
Cytoplasmic Foci 1.59 2.33 0.16 -2.88 -4.68 -1.57 1.69 -0.39 1.14 -1.25 -0.90 2.83 -3.47 -3.01 -4.72
Eisosomes -2.18 -10.52 -5.26 -3.95 4.39 -3.98 -15.30 -6.73 -5.59 0.43 -6.27 -19.68 -8.25 -5.77 3.22
Endoplasmic Reticulum -11.10 -7.77 -14.02 0.77 -5.69 -7.16 -7.23 -4.53 -2.61 -0.54 -10.94 -8.14 -11.66 -1.46 -0.29
Endosome -3.52 1.30 -3.78 -3.23 -3.93 -2.66 -1.44 -2.41 0.60 -2.01 -3.51 -0.55 -3.85 -2.81 -3.81
Golgi -2.32 -2.68 -2.69 -0.92 -2.57 -1.02 0.10 -0.46 0.89 -2.20 -2.14 -0.01 -2.53 -1.36 -2.60
Lipid Particles 0.31 3.36 -1.04 -1.13 -1.77 -0.97 -1.36 -1.28 -1.07 -0.96 -2.02 -0.11 -1.70 -1.42 -1.69
Mitochondria -2.73 -5.42 -3.79 -2.04 -2.27 -4.00 -10.46 -9.19 1.41 -3.91 -5.16 -13.69 -4.65 -0.87 -2.76
Mitotic Spindle -2.96 -5.50 -6.11 -3.93 -5.86 -3.88 -2.12 -2.14 -1.37 -1.85 -4.35 -1.96 -6.01 -3.44 -5.12
None 1.87 5.03 3.29 3.66 -7.78 0.09 -2.76 -3.45 -3.43 -1.34 -3.27 2.97 -3.01 -0.15 -5.77
Nuclear Periphery -3.44 -1.24 -8.54 -6.69 -8.37 -3.68 -5.25 -5.34 0.44 -4.06 -4.02 -4.47 -8.80 -1.39 -7.42
Nuclear Periphery Foci -3.79 2.01 -1.22 3.30 -5.50 -1.71 -0.51 -0.92 -0.53 -0.86 -3.77 0.26 -2.43 1.60 -2.33
Nucleolus -0.51 1.12 8.03 16.16 7.28 3.25 4.23 12.65 8.72 5.22 -0.64 2.81 15.49 17.22 8.45
Nucleus 0.67 -2.25 2.77 3.18 7.43 6.74 4.95 4.10 1.82 2.39 9.70 4.65 12.80 5.74 8.90
Peroxisomes -1.41 -3.14 -0.80 0.51 0.99 -1.88 -4.62 -2.01 -0.43 0.89 -2.39 -7.28 -1.66 -0.31 -0.33
Vacuole -2.85 -6.50 -6.98 -6.05 -5.90 -3.99 -4.24 -2.74 -2.12 -2.02 -4.79 -5.18 -7.49 -5.69 -5.10
Vacuole Periphery -2.94 -4.74 -3.05 -2.27 -2.78 -3.65 0.54 -1.03 3.43 -2.27 -4.07 -0.09 -2.90 -0.90 -2.91
Standard name
Human Ortholog
Description U1 snRNP protein involved in splicing; contains multiple tetriatricopeptide repeats
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Prp39

Prp39


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp39-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available