Standard name
Human Ortholog
Description Transcriptional regulator involved in the repression of GAL genes; involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.98 0.97 0.94 0.86 0.8 0.74 0.74 0.65 0.59 0.54 1.0 0.99 0.99 0.89 0.89 0.85 0.7 0.87 0.75 0.88 0.87 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.14 0.34 0.36 0.41 0.43 0.57 0.58 0.62 0 0 0 0 0 0 0.15 0 0.11 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 6 13 3 1 3
Bud 2 3 3 2 6 13 30 20 20 28 33 16 0 0 0 0 1 1 0 15 7 2 5 7
Bud Neck 0 1 5 0 5 2 2 1 6 4 3 2 5 3 3 2 1 0 0 5 1 0 0 2
Bud Site 1 0 1 0 0 0 1 1 2 5 12 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 4 5 3 2 8 2 2 4 5 5 2 3 3 5 0 5 0 1 9 1 1 1
Cytoplasm 109 687 770 463 563 763 786 650 465 609 363 401 530 421 509 420 280 324 91 635 558 376 380 324
Endoplasmic Reticulum 0 3 0 0 1 0 0 0 0 0 0 3 0 2 3 42 9 24 3 0 11 0 1 3
Endosome 1 6 2 2 7 2 2 3 3 1 1 0 1 0 1 21 11 28 1 11 10 3 2 4
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 0 0 1 2 0
Mitochondria 5 11 17 28 87 306 350 364 270 530 354 457 1 0 1 6 7 10 19 9 78 13 13 7
Nucleus 0 1 0 2 0 1 1 4 2 3 2 3 0 0 2 2 4 5 0 2 0 1 2 1
Nuclear Periphery 0 0 0 0 0 0 2 4 0 2 1 0 0 0 0 0 0 1 1 0 0 1 1 2
Nucleolus 0 0 0 0 1 1 1 2 1 0 0 0 0 0 0 1 0 1 0 0 2 0 0 0
Peroxisomes 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0
SpindlePole 0 1 4 0 6 9 11 19 4 10 19 13 0 1 1 7 3 13 2 15 14 8 11 11
Vac/Vac Membrane 0 12 1 2 5 14 21 17 5 16 17 18 0 0 2 25 19 25 1 9 4 4 4 3
Unique Cell Count 113 715 782 475 600 889 977 884 631 937 611 739 531 424 515 470 315 380 131 728 740 427 439 380
Labelled Cell Count 121 727 808 504 684 1113 1217 1087 780 1212 810 924 539 430 525 533 336 440 131 728 740 427 439 380


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 4.3 3.6 3.4 3.6 3.2 3.1 3.1 3.0 2.7 2.6 2.7 5.1 5.1 4.9 5.8 5.3 5.5 5.2 5.5 5.8
Std Deviation (1e-4) 0.7 0.8 0.9 1.1 1.3 1.5 1.3 1.3 1.3 0.8 0.8 0.8 0.9 0.8 0.9 1.5 1.3 1.3 1.2 1.4 1.8
Intensity Change (Log2) – – – -0.08 -0.02 -0.18 -0.2 -0.22 -0.25 -0.4 -0.48 -0.42 0.51 0.49 0.43 0.69 0.56 0.62 0.53 0.62 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.2 6.6 7.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1363 2.9621 2.5064 2.3179 2.0816 2.6135 -0.4019 -0.0924 -0.3343 -0.1832 -0.0057 -0.1654 -0.7341 -0.1375 -0.4079 -0.5528 -0.9968 -0.34
Actin 0.0002 0.0002 0.0003 0.0013 0.0001 0.0002 0.0176 0.0001 0.0002 0.0016 0.0008 0.0007 0.0071 0.0001 0.0005 0.0009 0.0006 0.0003
Bud 0.0013 0.0001 0 0.0003 0 0.0036 0.0004 0.007 0.0025 0.0012 0.0021 0.0015 0.0004 0 0.0005 0.0019 0 0.0001
Bud Neck 0.0002 0 0.0001 0.0237 0 0.0001 0.0042 0.0002 0.001 0.0224 0.0002 0.0007 0.0005 0 0.0001 0.0004 0 0.0001
Bud Periphery 0.0005 0.0001 0.0001 0.0005 0 0.001 0.0008 0.002 0.0011 0.0046 0.0025 0.0008 0.0007 0 0.0005 0.0024 0 0.0001
Bud Site 0.0014 0.0001 0.0004 0.0002 0 0 0.0016 0.0158 0.0056 0.0063 0.0004 0.0002 0.0056 0.0003 0.0009 0.0004 0 0
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0 0.0005 0.0004 0.0001 0.0018 0.0001 0.0001 0.0002 0 0.0001 0.0002 0 0
Cytoplasm 0.0338 0.0645 0.073 0.0509 0.0299 0.0625 0.0818 0.0897 0.0875 0.1014 0.0598 0.1004 0.045 0.1049 0.1041 0.1063 0.1003 0.1276
Cytoplasmic Foci 0.0148 0.0026 0.0113 0.0061 0.0012 0.006 0.016 0.0041 0.0056 0.0043 0.005 0.0098 0.004 0.0061 0.0033 0.0057 0.0064 0.0083
Eisosomes 0.0003 0.0003 0.0003 0.0001 0 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0007 0.0014 0.001 0.0004 0.0003 0.0003 0.0034 0.0006 0.0008 0.0011 0.0008 0.0007 0.0008 0.0009 0.0008 0.0015 0.0019 0.0005
Endosome 0.0008 0.0017 0.0007 0.0004 0.0001 0.0002 0.008 0.0008 0.0012 0.0015 0.0005 0.0012 0.0056 0.0033 0.0006 0.0012 0.0017 0.0034
Golgi 0.0001 0.0001 0.0002 0.0001 0 0 0.0013 0.0001 0.0001 0.0003 0.0001 0.0001 0.0006 0.0001 0.0001 0.0002 0.0001 0.0001
Lipid Particles 0.0005 0.0005 0.001 0.0001 0.0001 0.0001 0.002 0.0001 0.0002 0.0003 0.0003 0.0009 0.0024 0.0002 0.0001 0.001 0.0002 0.0005
Mitochondria 0.002 0.0006 0.0002 0.0002 0.0001 0.0004 0.0012 0.0003 0.0004 0.0006 0.0005 0.0004 0.0005 0.0001 0.0003 0.0005 0.0001 0.0001
None 0.913 0.9254 0.9065 0.8892 0.9674 0.9236 0.8344 0.8735 0.8865 0.8344 0.9192 0.8714 0.9185 0.8815 0.8857 0.8637 0.8861 0.8556
Nuclear Periphery 0.0004 0.0005 0.0002 0.0002 0.0001 0.0001 0.0058 0.0003 0.0005 0.0021 0.0012 0.0008 0.0006 0.0005 0.0004 0.0013 0.0006 0.0002
Nucleolus 0.0014 0.0001 0.0002 0.0003 0 0.0001 0.0024 0.0004 0.0006 0.0008 0.0005 0.0003 0.0016 0.0001 0.0001 0.0009 0.0001 0.0001
Nucleus 0.019 0.0005 0.0006 0.0018 0.0001 0.0008 0.0052 0.0021 0.0031 0.0038 0.0015 0.0019 0.0006 0.0006 0.0007 0.0041 0.0006 0.0006
Peroxisomes 0.0003 0.0001 0.0004 0.0001 0.0001 0 0.0014 0.0001 0.0002 0.0003 0.0003 0.0032 0.0006 0 0.0001 0.0004 0.0001 0.0001
Punctate Nuclear 0.0088 0.0003 0.0024 0.0237 0.0001 0.0006 0.0097 0.0016 0.0023 0.0095 0.0031 0.0043 0.0004 0.0009 0.0007 0.0059 0.0008 0.0014
Vacuole 0.0005 0.0007 0.001 0.0003 0.0001 0.0002 0.0015 0.0006 0.0005 0.0016 0.0007 0.0003 0.0036 0.0003 0.0003 0.0008 0.0003 0.0006
Vacuole Periphery 0.0001 0.0001 0 0 0 0 0.0005 0.0001 0.0001 0.0003 0.0002 0.0001 0.0004 0 0.0001 0.0002 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.8522 24.1315 23.5025 36.151 37.1872 37.097 30.3086 29.5908 25.2511 41.2162
Translational Efficiency 0.1275 0.1257 0.1275 0.0855 0.0747 0.1032 0.1242 0.1125 0.1328 0.0874

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transcriptional regulator involved in the repression of GAL genes; involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding
Localization
Cell Percentages cytoplasm (11%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gal80

Gal80


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gal80-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available