Standard name
Human Ortholog
Description Subunit of the THO complex; THO is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.14 0.17 0.12 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0.08 0.13 0.16 0.16 0.18 0.26 0.26 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.88 0.93 0.87 0.89 0.85 0.82 0.76 0.69 0.75 0.69 0.7 0.71 0.91 0.81 0.76 0.82 0.87 0.85 0.84 0.9 0.76 0.85 0.78 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.3 0.2 0.23 0.21 0.23 0.3 0.35 0.38 0.38 0.39 0.47 0.38 0.08 0 0.06 0.05 0.1 0.11 0.11 0.05 0.17 0.07 0.1 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1
Bud 1 0 0 0 1 0 4 1 1 5 2 3 0 0 2 0 3 0 0 0 1 1 4 1
Bud Neck 0 0 0 0 1 0 1 1 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 0 0 1 8 9 11 16 12 12 0 0 2 1 2 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 2 10 9 4 5 10 16 8 12 3 6 29 58 88 53 19 22 1 0 0 2 2 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 6 3 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 1 1 0 0 0 0 3 2 3 4 0 3 1 1 2 1 3 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 2 0 1 1 0 4
Mitochondria 10 9 7 6 22 46 89 88 68 138 76 82 0 0 1 8 6 6 0 2 3 0 1 3
Nucleus 198 174 247 194 238 292 427 387 277 371 202 278 461 336 388 369 385 271 200 180 229 258 254 261
Nuclear Periphery 0 0 1 1 0 3 10 5 7 3 1 3 3 3 2 4 5 5 0 0 0 0 0 2
Nucleolus 67 38 64 46 63 109 194 216 141 209 136 147 43 14 29 21 45 36 25 9 52 21 33 56
Peroxisomes 0 0 0 0 0 0 0 0 0 2 0 0 1 1 4 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 1 8 8 3 2 0 0 0 1 1 2 5
Vac/Vac Membrane 1 0 0 0 2 0 5 1 1 0 3 1 3 13 13 9 10 9 5 3 5 11 15 25
Unique Cell Count 224 188 284 219 279 358 562 562 370 538 287 390 508 416 510 448 441 320 239 200 301 303 324 375
Labelled Cell Count 281 223 330 256 331 456 749 725 515 759 435 532 544 435 541 481 484 356 239 200 301 303 324 375


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 9.1 8.0 7.8 7.3 6.7 6.3 5.9 6.1 6.0 5.7 5.9 10.6 11.0 9.6 10.9 10.5 9.3 11.4 11.3 12.2
Std Deviation (1e-4) 1.5 1.9 2.2 2.2 1.9 2.0 1.9 1.8 1.7 1.7 1.8 1.6 3.1 4.2 3.6 3.0 3.0 2.7 3.3 2.9 3.1
Intensity Change (Log2) – – – -0.03 -0.14 -0.27 -0.34 -0.45 -0.39 -0.42 -0.48 -0.45 0.4 0.45 0.27 0.45 0.39 0.22 0.51 0.49 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2.5 2.5 3.1 2.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -1.6 -1.8 -1.6 -0.5 -1.1 -1.1 -2.0 -1.7 1.4 4.6 5.6 3.9 0.5 1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 2.9 4.7 5.8 5.7 6.3 8.3 8.1 7.0 0 0 0 -0.6 -1.1 -0.5
Nucleus 0.5 -0.6 -1.9 -3.8 -5.7 -3.8 -5.7 -4.8 -4.8 1.7 -2.2 -3.7 -1.7 0.1 -0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.4 0 2.2 3.6 4.6 4.3 4.7 6.2 4.2 -5.6 -7.9 -7.1 -7.3 -4.5 -3.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 3.0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2092 5.5392 5.4709 5.3282 4.8793 5.8553 5.1595 5.8172 5.513 5.2557 4.6557 5.0765 4.6415 4.6436 4.1931 4.2527 4.104 4.2872
Actin 0.0402 0.0004 0.0061 0.0104 0.005 0.0002 0.0145 0 0.0037 0.0009 0.0043 0.0026 0.0163 0 0.0024 0.0267 0.0063 0.0002
Bud 0.001 0 0.0001 0.0009 0.0004 0 0.001 0 0.0001 0.0001 0.0002 0.0001 0.0007 0.0001 0.0002 0.0007 0.0004 0
Bud Neck 0.0013 0.0001 0.0003 0.0012 0.001 0.0005 0.001 0.0001 0.0009 0.0003 0.0008 0.0005 0.0018 0.0001 0.0003 0.0019 0.001 0.0005
Bud Periphery 0.0025 0.0001 0.0001 0.0013 0.0011 0.0001 0.0022 0 0.0002 0.0001 0.0005 0.0001 0.0015 0.0001 0.0004 0.0014 0.0009 0.0001
Bud Site 0.0029 0.0001 0.0002 0.0306 0.0012 0.0001 0.0084 0.0002 0.0015 0.0008 0.0006 0.0002 0.0023 0.0004 0.0049 0.0022 0.0016 0.0001
Cell Periphery 0.001 0 0 0.0011 0.0005 0 0.0005 0 0.0002 0.0001 0.0004 0 0.0007 0 0.0001 0.0005 0.0004 0
Cytoplasm 0.0027 0.0048 0.0005 0.0006 0.0028 0.0001 0.0174 0.0003 0.0079 0.0007 0.0074 0.0014 0.0061 0.0001 0.0024 0.0084 0.0021 0.0004
Cytoplasmic Foci 0.0045 0.0001 0.0034 0.0027 0.0133 0.0045 0.012 0 0.0022 0.0047 0.0021 0.002 0.0097 0.0001 0.0066 0.0299 0.0146 0.0007
Eisosomes 0.0004 0.0001 0.0001 0.0002 0.0001 0 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0.0004 0.0001 0
Endoplasmic Reticulum 0.0038 0.0002 0.0005 0.0006 0.0045 0.0001 0.0027 0 0.0011 0.0003 0.0055 0.0016 0.0055 0.0001 0.0017 0.0052 0.0028 0.0007
Endosome 0.0133 0.0009 0.004 0.01 0.0159 0.0004 0.0218 0 0.0012 0.0057 0.0131 0.0053 0.0109 0.0003 0.007 0.0163 0.045 0.002
Golgi 0.0036 0.0001 0.0013 0.0054 0.0109 0.0002 0.0051 0 0.0003 0.0031 0.0014 0.0016 0.0042 0 0.0024 0.0085 0.0293 0.0004
Lipid Particles 0.0078 0 0.0065 0.0095 0.0414 0.0025 0.0172 0 0.0014 0.0137 0.012 0.0026 0.0143 0.0001 0.0033 0.0187 0.036 0.0056
Mitochondria 0.0112 0.0066 0.0007 0.0177 0.0105 0.0006 0.0207 0.0001 0.0004 0.0026 0.0029 0.0007 0.0103 0.0004 0.0031 0.022 0.0223 0.0007
None 0.0032 0.0006 0.0019 0.0003 0.0014 0.0001 0.0103 0 0.0005 0.0006 0.0028 0.0004 0.003 0.0001 0.0086 0.0033 0.0012 0.0002
Nuclear Periphery 0.0227 0.0162 0.0101 0.0007 0.0122 0.0005 0.0304 0.0023 0.0027 0.0034 0.0455 0.0037 0.0148 0.0008 0.0086 0.0111 0.0172 0.001
Nucleolus 0.0436 0.0264 0.0258 0.0076 0.0741 0.0933 0.0329 0.0259 0.0232 0.0297 0.1252 0.0979 0.0535 0.0211 0.0216 0.037 0.1528 0.0805
Nucleus 0.789 0.9394 0.9279 0.8824 0.7265 0.8942 0.7651 0.9673 0.9468 0.9086 0.7527 0.8756 0.8123 0.9751 0.9178 0.7755 0.6421 0.9036
Peroxisomes 0.0141 0 0.0032 0.0018 0.0054 0.0011 0.0072 0 0.0001 0.0058 0.0003 0.0012 0.0056 0 0.0021 0.0105 0.0042 0.0007
Punctate Nuclear 0.0214 0.0022 0.0064 0.0004 0.0656 0.0013 0.0209 0.003 0.0051 0.0181 0.0097 0.0022 0.0146 0.0006 0.0035 0.0133 0.0098 0.0018
Vacuole 0.0057 0.0012 0.0004 0.0098 0.0038 0.0001 0.0046 0.0004 0.0004 0.0005 0.0077 0.0002 0.0083 0.0004 0.0015 0.004 0.0064 0.0004
Vacuole Periphery 0.0041 0.0004 0.0005 0.0048 0.0022 0.0001 0.0036 0.0002 0.0002 0.0002 0.0048 0.0002 0.0034 0.0001 0.0015 0.0024 0.0034 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.2115 13.3859 7.4497 10.0897 7.8872 2.8061 7.6132 8.2785 11.6228 9.1551
Translational Efficiency 5.2236 1.6017 2.5401 2.5403 2.5406 6.7094 3.0923 2.3196 1.9982 2.2313

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the THO complex; THO is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Mft1

Mft1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mft1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available