Standard name
Human Ortholog
Description Dihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 – 0 0 0 0.05 0.08 0.05 0.09 0.09 0.12 – 0 0 0.11 0.1 0.08 0 0 0 0 0 0
Cytoplasm 0.99 0.95 – 0.99 0.98 0.96 0.88 0.83 0.83 0.74 0.83 0.75 – 0.98 0.99 0.96 0.97 0.97 0.97 0.9 0.74 0.75 0.97 0.95
Endoplasmic Reticulum 0 0 – 0 0 0 0.07 0.11 0.05 0.09 0.06 0.08 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0 0 0 0 0 0.1 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.09 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0.05 0.11 0 0.08 – 0 0 0 0 0 0 0.05 0.09 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 1 5 6 10 13 3 13 – 0 0 0 0 0 0 0 0 1 0 0
Bud 0 4 – 0 0 0 2 1 3 2 1 2 – 0 0 0 0 0 0 6 0 0 0 2
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 2 20 – 6 14 8 22 41 13 33 23 30 – 2 6 36 44 32 0 0 0 0 0 0
Cytoplasm 181 254 – 148 360 255 367 436 227 275 209 182 – 56 151 306 411 391 177 252 39 27 75 183
Endoplasmic Reticulum 0 4 – 0 2 5 30 58 14 34 15 19 – 0 1 7 18 14 0 1 1 0 0 1
Endosome 0 0 – 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0
Golgi 0 1 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1 0 – 0 0 0 6 4 9 14 26 10 – 1 0 1 0 0 0 0 0 0 0 0
Nucleus 1 6 – 3 2 1 4 5 4 2 0 0 – 1 0 0 1 2 0 3 3 3 0 1
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 – 1 2 0 8 17 13 40 9 19 – 0 0 5 8 13 1 15 4 0 0 1
Unique Cell Count 183 268 150 366 265 419 525 272 371 251 242 57 152 319 425 405 183 281 54 37 78 193
Labelled Cell Count 185 291 158 380 270 445 568 293 413 286 276 60 158 355 482 452 183 281 54 37 78 193


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.9 19.4 – 14.7 16.5 15.7 15.8 15.7 14.2 15.1 14.0 13.9 – 17.4 20.4 33.3 31.5 28.1 – 18.8 18.8
Std Deviation (1e-4) 3.3 5.4 – 3.1 3.6 3.3 3.4 4.0 3.9 4.3 4.3 4.1 – 5.7 5.4 13.2 10.8 10.8 – 5.2 5.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.7189 17.7881 16.0639 15.8626 13.8403 14.8278 9.25 12.5037 13.4324 11.9316 10.8636 12.4223 7.708 11.0648 9.9388 11.0794 7.916 10.3009
Actin 0.0122 0.0014 0.0014 0.0002 0.0015 0.0016 0.01 0.0096 0.0087 0.0062 0.0305 0.0021 0.0167 0.0005 0.0095 0.0001 0.0582 0.0005
Bud 0.0014 0.002 0.0083 0.0004 0.0015 0.0038 0.0004 0.0017 0.0008 0.0018 0.001 0.0006 0.0005 0.0002 0.0014 0.0001 0.0042 0.0036
Bud Neck 0.001 0.0004 0.0006 0.0005 0.0016 0.0016 0.001 0.0003 0.0004 0.001 0.0006 0.0018 0.0011 0.0001 0.0005 0.0002 0.0009 0.0006
Bud Periphery 0.0008 0.0008 0.0018 0.0002 0.0009 0.0022 0.0005 0.0011 0.0011 0.0029 0.0014 0.0004 0.0007 0.0001 0.0004 0 0.0014 0.001
Bud Site 0.0027 0.0043 0.0033 0.0002 0.0016 0.0015 0.0043 0.0043 0.0022 0.0013 0.0043 0.0017 0.002 0.0007 0.0009 0 0.0019 0.0002
Cell Periphery 0.001 0.0002 0.0007 0.0001 0.0002 0.0001 0.0007 0.0002 0.0001 0.0003 0.0004 0.0001 0.0002 0.0001 0.0001 0 0.0003 0.0001
Cytoplasm 0.9044 0.9732 0.958 0.9498 0.9162 0.9433 0.7684 0.9458 0.937 0.907 0.6232 0.8681 0.8657 0.9768 0.9537 0.9877 0.8081 0.972
Cytoplasmic Foci 0.0082 0.0012 0.0033 0.0022 0.0174 0.0084 0.0266 0.0062 0.0147 0.0071 0.037 0.0306 0.0227 0.0038 0.008 0.0023 0.0122 0.0042
Eisosomes 0.0004 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.0005 0
Endoplasmic Reticulum 0.0137 0.0047 0.0068 0.0078 0.0033 0.0127 0.009 0.0015 0.002 0.0035 0.0046 0.0015 0.0091 0.0009 0.0019 0.0013 0.0051 0.0038
Endosome 0.0136 0.0029 0.0035 0.0048 0.0133 0.0075 0.034 0.0025 0.0064 0.0222 0.0681 0.0279 0.0274 0.0018 0.0045 0.0024 0.0209 0.0051
Golgi 0.0022 0.0001 0.0004 0.0001 0.0005 0.0006 0.0061 0.0012 0.0012 0.004 0.0465 0.0111 0.0038 0.0002 0.0016 0 0.0058 0.0002
Lipid Particles 0.0026 0.0001 0.0001 0 0.0004 0.0001 0.028 0.0007 0.0015 0.0094 0.0128 0.0148 0.0049 0.0001 0.0007 0 0.0148 0
Mitochondria 0.0036 0.0001 0.0008 0.0002 0.0008 0.0014 0.0054 0.0005 0.0021 0.0087 0.0215 0.0109 0.0063 0.0001 0.0005 0.0001 0.0021 0.0006
None 0.008 0.0007 0.0014 0.0007 0.0027 0.0037 0.0141 0.018 0.0092 0.002 0.0009 0.0008 0.0093 0.0112 0.0106 0.0005 0.0131 0.0024
Nuclear Periphery 0.005 0.0014 0.0011 0.0017 0.0028 0.0026 0.0225 0.0009 0.0045 0.0029 0.0107 0.0019 0.0092 0.0005 0.0012 0.0018 0.0105 0.0008
Nucleolus 0.0007 0 0.0003 0 0.0026 0.0001 0.008 0.0001 0.0002 0.0031 0.0006 0.0052 0.0004 0.0001 0.0001 0 0.0018 0.0002
Nucleus 0.01 0.0028 0.0041 0.0029 0.0214 0.0045 0.013 0.0012 0.0026 0.0046 0.0054 0.0027 0.004 0.0013 0.0019 0.0017 0.0112 0.0028
Peroxisomes 0.0019 0 0.0001 0 0.0002 0.0001 0.031 0.0016 0.0021 0.002 0.0674 0.0043 0.0036 0.0001 0.0007 0 0.003 0
Punctate Nuclear 0.0017 0.0001 0.0004 0.0001 0.0026 0.0008 0.0027 0.0009 0.0017 0.0054 0.0013 0.0081 0.0084 0.0004 0.0007 0.0001 0.0175 0.0003
Vacuole 0.004 0.0031 0.0033 0.0268 0.0078 0.0026 0.0112 0.0013 0.001 0.0034 0.0395 0.0043 0.0028 0.001 0.0012 0.0013 0.0049 0.0011
Vacuole Periphery 0.0009 0.0005 0.0005 0.0014 0.0009 0.0006 0.0029 0.0002 0.0005 0.0012 0.0221 0.001 0.0011 0.0001 0.0003 0.0004 0.0016 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.0183 51.7075 51.715 64.2517 57.0615 53.4117 65.4938 73.0659 75.4787 74.6066
Translational Efficiency 1.7909 2.2202 1.8866 1.8278 2.0504 2.7501 1.7359 1.4342 1.7444 1.6164

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1476 1767 80 1544 227 2052 128 1706 1703 3819 208 3250

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1230.66 1022.01 2262.09 1978.14 978.34 1205.62 2242.08 2300.56 1197.03 1120.67 2249.78 2147.39
Standard Deviation 245.53 254.90 577.51 674.47 306.72 230.29 681.07 677.89 268.60 258.73 643.29 695.17
Intensity Change Log 2 — -0.268023 0.878224 0.684712 — 0.301367 1.196430 1.233577 — 0.012116 1.027868 0.953779

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000084 0.000103 0.000709 0.000481 0.000358 0.000127 0.000594 0.000510 0.000120 0.000116 0.000639 0.000496
Bud Neck 0.000336 0.000984 0.005945 0.001313 0.001218 0.000392 0.004969 0.001244 0.000454 0.000666 0.005344 0.001277
Bud Site 0.006414 0.009419 0.009694 0.012610 0.023761 0.007904 0.011242 0.011655 0.008726 0.008605 0.010646 0.012109
Cell Periphery 0.000093 0.000097 0.003980 0.001886 0.000324 0.000066 0.008458 0.001305 0.000124 0.000080 0.006736 0.001581
Cytoplasm 0.913818* 0.880395* 0.714016* 0.866813* 0.787017* 0.913915* 0.713049* 0.899609* 0.896916* 0.898406* 0.713421* 0.884028*
Cytoplasmic Foci 0.047035 0.048777 0.045840 0.010493 0.091816 0.039537 0.032077 0.008006 0.053004 0.043812 0.037371 0.009187
Eisosomes 0.000002 0.000005 0.000006 0.000005 0.000014 0.000003 0.000005 0.000002 0.000004 0.000004 0.000006 0.000004
Endoplasmic Reticulum 0.001152 0.000740 0.000767 0.000203 0.005285 0.000417 0.000569 0.000132 0.001703 0.000566 0.000645 0.000166
Endosome 0.001535 0.004077 0.004965 0.003619 0.006728 0.002127 0.004764 0.002116 0.002228 0.003029 0.004841 0.002830
Golgi 0.003735 0.003117 0.020709 0.008011 0.008554 0.003843 0.025612 0.004035 0.004377 0.003507 0.023727 0.005924
Lipid Particles 0.000479 0.000701 0.012476 0.005052 0.002235 0.000419 0.018151 0.004531 0.000713 0.000549 0.015968 0.004779
Mitochondria 0.000113 0.000684 0.000106 0.001045 0.000317 0.000453 0.000044 0.000246 0.000140 0.000560 0.000068 0.000626
Mitotic Spindle 0.002150 0.001003 0.008637 0.005910 0.002149 0.002445 0.007040 0.003223 0.002150 0.001777 0.007654 0.004500
None 0.004356 0.006647 0.001340 0.001540 0.012117 0.001608 0.001010 0.001421 0.005391 0.003939 0.001137 0.001478
Nuclear Periphery 0.000020 0.000097 0.000723 0.000079 0.000403 0.000022 0.000435 0.000057 0.000071 0.000056 0.000546 0.000067
Nuclear Periphery Foci 0.001333 0.000960 0.020749 0.002062 0.003616 0.000548 0.016262 0.001655 0.001637 0.000738 0.017988 0.001848
Nucleolus 0.000096 0.000726 0.000127 0.000174 0.001085 0.000114 0.000096 0.000114 0.000228 0.000397 0.000108 0.000143
Nucleus 0.007269 0.014178 0.061514 0.016322 0.029112 0.006508 0.035334 0.014880 0.010181 0.010057 0.045403 0.015565
Peroxisomes 0.000148 0.000389 0.000079 0.000293 0.000456 0.000299 0.000090 0.000237 0.000189 0.000341 0.000086 0.000264
Vacuole 0.007881 0.025037 0.083758 0.058666 0.016326 0.017813 0.114854 0.043347 0.009006 0.021155 0.102894 0.050624
Vacuole Periphery 0.001951 0.001867 0.003860 0.003424 0.007109 0.001441 0.005344 0.001673 0.002638 0.001638 0.004773 0.002505

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.65 -5.48 -3.57 -3.38 2.36 2.95 -2.53 -1.02 -2.72 0.76 0.30 -7.82 -4.07 -4.13 2.07
Bud Neck -4.40 -6.89 -9.69 -3.73 5.53 3.16 -5.75 -1.44 -8.44 5.41 -2.31 -9.67 -10.15 -8.61 7.68
Bud Site -3.85 -2.72 -5.62 -2.94 -0.62 3.40 2.43 2.45 -3.04 -0.08 0.14 -2.10 -3.31 -4.23 -0.46
Cell Periphery -0.26 -4.89 -6.70 -6.68 2.63 5.52 -3.16 -4.69 -6.34 2.82 4.28 -4.09 -8.87 -9.18 3.24
Cytoplasm 6.28 9.53 9.95 4.48 -6.86 -7.81 3.47 -5.42 6.75 -7.92 -0.11 12.12 6.39 7.94 -10.27
Cytoplasmic Foci -0.56 -0.57 13.40 16.99 5.18 6.11 6.29 9.96 15.75 6.91 3.11 2.57 16.77 23.09 8.18
Eisosomes -4.50 -4.70 -1.90 0.41 1.47 2.84 2.10 2.92 0.60 4.39 -0.43 -3.19 0.13 0.55 3.12
Endoplasmic Reticulum 1.22 1.09 3.83 2.44 3.98 2.33 2.27 2.48 3.34 4.13 3.05 2.87 4.38 3.58 5.66
Endosome -5.43 -4.42 -2.23 1.37 2.23 4.07 1.36 3.99 0.42 3.74 -2.59 -5.19 -0.47 1.30 4.11
Golgi 2.00 -4.17 -2.41 -3.02 3.24 2.63 -4.27 2.81 0.53 6.12 2.05 -7.09 -0.69 -1.98 6.74
Lipid Particles -1.76 -5.88 -10.09 -9.46 3.45 3.02 -5.82 -3.04 -7.49 4.96 1.52 -8.22 -11.09 -11.72 5.91
Mitochondria -1.29 -0.31 -1.44 -0.23 -1.42 -0.64 3.54 1.05 1.20 -2.16 -1.83 2.84 -1.51 0.16 -1.79
Mitotic Spindle 1.37 -1.96 -2.20 -3.36 1.00 -0.30 -1.38 -1.06 -0.78 1.07 0.47 -2.20 -2.16 -3.04 1.36
None -1.75 4.44 4.36 4.46 -0.07 2.06 2.18 2.11 0.97 -1.20 1.49 4.77 4.49 4.55 -1.10
Nuclear Periphery -2.30 -7.05 -4.14 0.09 6.32 2.31 -0.53 1.98 -5.08 5.60 0.53 -8.09 -0.56 -1.53 8.28
Nuclear Periphery Foci 1.03 -5.48 -2.51 -6.08 5.25 3.89 -6.54 2.15 -6.62 7.94 2.90 -9.28 -1.26 -9.06 9.16
Nucleolus -2.07 -0.83 -1.14 1.88 -0.59 2.26 2.30 2.23 -0.43 -1.90 -1.00 1.68 1.26 1.78 -1.68
Nucleus -5.96 -6.05 -12.40 -5.17 4.79 3.62 -1.03 1.50 -13.85 3.37 0.04 -7.45 -9.04 -12.28 5.66
Peroxisomes -7.16 1.82 -2.08 2.11 -2.85 1.66 3.80 2.32 1.61 -3.45 -5.48 3.86 -1.55 2.62 -4.36
Vacuole -9.89 -6.00 -18.16 -11.96 1.40 -0.76 -6.88 -11.65 -13.45 4.15 -10.13 -9.27 -24.31 -17.94 4.21
Vacuole Periphery 0.22 -1.39 -1.70 -2.36 0.46 2.42 0.75 2.39 -0.16 3.68 2.10 -2.20 0.94 -1.97 3.15
Standard name
Human Ortholog
Description Dihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Dak1

Dak1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dak1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available