Standard name
Human Ortholog
Description Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0.07 0.11 0 0 0 0.09 0 0 0 0 – – – 0.06 0.13 0.07 0 0 0 0.05 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.07 0 0.07 0 0.05 0.06 0 0 0 0 – – – 0.06 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0.07 0.08 0.05 0.05 0 0.06 0.12 0.06 0 0 0.05 – – – 0 0.05 0 0 0 0 0 0 0
Endosome 0.07 0.08 0.12 0.13 0.16 0.13 0.1 0.06 0.06 0 0 0 – – – 0.14 0.11 0.19 0.07 0.09 0.11 0.09 0.06 0.08
Golgi 0.34 0.61 0.65 0.61 0.52 0.21 0.2 0.16 0.07 0.11 0 0.07 – – – 0.54 0.72 0.47 0.77 0.75 0.6 0.6 0.68 0.66
Mitochondria 0.83 0.52 0.24 0.54 0.42 0.74 0.78 0.71 0.85 0.82 0.93 0.88 – – – 0.12 0.11 0.13 0.08 0.09 0.15 0.08 0.07 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.1 0.15 0 0 0.08 0 0 0 0.05 0 0.05 – – – 0.21 0.12 0.29 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 24 9 12 7 13 12 23 10 13 6 9 – – – 18 40 20 9 10 7 0 0 1
Bud 2 2 0 1 2 7 9 7 6 5 10 13 – – – 1 5 4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 1 0 – – – 0 0 0 – – – – – –
Cell Periphery 3 0 0 0 1 1 5 4 3 6 4 4 – – – 8 3 1 0 0 0 0 0 0
Cytoplasm 14 16 8 4 11 10 18 15 6 11 8 5 – – – 16 9 15 1 0 0 0 0 0
Endoplasmic Reticulum 17 23 10 5 8 14 19 32 19 13 7 14 – – – 10 14 10 1 1 2 0 0 1
Endosome 24 29 14 14 26 42 35 16 19 12 8 11 – – – 41 33 53 28 38 19 1 1 3
Golgi 122 214 78 64 82 69 67 42 22 31 12 20 – – – 153 223 131 296 306 104 10 15 29
Mitochondria 295 183 29 57 67 246 263 189 256 239 289 243 – – – 35 33 35 30 34 26 1 1 2
Nucleus 0 0 0 0 0 0 0 1 0 2 2 0 – – – 1 0 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 1 0 1 0 0 2 1 3 1 – – – 2 1 0 1 0 0 0 0 0
Nucleolus 3 2 0 1 0 1 0 1 0 3 4 2 – – – 2 1 1 0 0 0 0 0 0
Peroxisomes 2 0 0 1 0 2 12 1 1 4 5 0 – – – 0 0 2 5 2 5 0 0 0
SpindlePole 1 1 0 1 5 5 3 10 5 4 1 4 – – – 8 4 8 2 0 0 0 0 0
Vac/Vac Membrane 19 35 18 2 6 25 14 4 9 14 8 13 – – – 60 36 81 3 11 6 0 0 1
Unique Cell Count 356 350 120 105 158 332 336 265 300 291 312 277 284 308 277 384 410 173 18 23 45
Labelled Cell Count 516 529 166 163 215 436 457 345 359 359 368 339 355 402 361 384 410 173 18 23 45


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 9.4 8.9 8.4 8.4 7.4 7.4 7.4 6.4 6.5 6.0 5.9 – – – 10.1 11.3 10.5 – – –
Std Deviation (1e-4) 1.0 1.2 1.2 1.2 1.4 1.0 1.5 1.3 1.1 1.0 1.1 0.9 – – – 2.1 2.0 2.0 – – –
Intensity Change (Log2) – – – -0.09 -0.09 -0.27 -0.27 -0.27 -0.48 -0.44 -0.57 -0.58 – – – 0.18 0.35 0.24 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.0 -1.1 -1.6 -1.8 0.4 -1.9 -1.2 0 -1.9 – – – -0.4 1.6 -0.1
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cytoplasm -1.0 0.1 0 -0.5 -0.4 0 -1.3 0 0 – – – -0.4 0 -0.5
Endoplasmic Reticulum -1.1 -1.1 -1.7 -1.0 1.1 -0.7 -1.6 0 -1.3 – – – -2.0 -1.5 -2.0
Endosome 0.4 1.1 0.3 -0.4 -1.9 -1.8 -2.9 -3.9 -2.9 – – – 0.7 -0.3 1.8
Golgi -0.6 -2.2 -8.9 -9.1 -9.6 -12.5 -11.3 -14.0 -12.3 – – – -2.1 1.5 -3.2
Mitochondria 4.6 3.2 9.6 10.6 8.6 12.1 11.2 14.5 12.5 – – – -3.0 -3.6 -2.9
Nucleus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole -3.4 -3.3 -2.4 -4.0 -5.3 -4.5 -3.5 -4.9 -3.5 – – – 1.4 -0.9 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4077 4.0483 3.8619 3.1855 3.5896 3.8699 2.8921 3.7282 3.3562 3.7306 3.3893 3.5291 3.406 4.3336 3.9985 3.8796 3.7663 4.109
Actin 0.0147 0.0084 0.0115 0.0016 0.0122 0.0075 0.0969 0.0091 0.0169 0.036 0.0072 0.0249 0.0324 0.0084 0.0118 0.0029 0.0071 0.0237
Bud 0.0005 0.0031 0.0043 0.0004 0.0004 0.0002 0.0007 0.0003 0.0007 0.0023 0.0003 0.0008 0.0009 0.0013 0.0011 0.0001 0.0002 0.0002
Bud Neck 0.0009 0.0003 0.0006 0.0004 0.0008 0.0006 0.01 0.0003 0.0014 0.0033 0.0009 0.0008 0.0009 0.0002 0.0003 0.0005 0.0002 0.0006
Bud Periphery 0.0007 0.001 0.0011 0.0005 0.0003 0.0002 0.0008 0.0001 0.001 0.0031 0.0004 0.0013 0.0009 0.0008 0.0011 0.0001 0.0004 0.0003
Bud Site 0.0041 0.0088 0.0026 0.0005 0.0008 0.0004 0.005 0.0008 0.003 0.0116 0.0055 0.0013 0.0034 0.0051 0.0019 0.0002 0.0004 0.0002
Cell Periphery 0.0017 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0 0.0004 0.0003 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0076 0.0066 0.0058 0.005 0.0047 0.0031 0.0061 0.0149 0.0085 0.0058 0.003 0.0048 0.0083 0.0104 0.0067 0.001 0.0012 0.0038
Cytoplasmic Foci 0.0896 0.118 0.0861 0.0579 0.0811 0.0712 0.1074 0.1247 0.1037 0.0862 0.072 0.071 0.0969 0.1003 0.0723 0.0405 0.0723 0.0691
Eisosomes 0.0004 0 0.0002 0 0 0 0.0005 0 0.0003 0.0001 0.0001 0.0005 0.0004 0 0.0003 0 0.0001 0.0001
Endoplasmic Reticulum 0.0031 0.001 0.002 0.0013 0.0016 0.0018 0.0043 0.0012 0.0031 0.0012 0.0014 0.0046 0.0021 0.0017 0.006 0.0007 0.0012 0.0014
Endosome 0.3198 0.3681 0.3093 0.3909 0.3898 0.2449 0.2768 0.3465 0.2669 0.3254 0.2705 0.2249 0.2909 0.303 0.2598 0.2609 0.2791 0.2129
Golgi 0.432 0.4259 0.4498 0.4227 0.4337 0.5723 0.3662 0.462 0.5093 0.4707 0.5738 0.5742 0.4382 0.5358 0.561 0.5888 0.5773 0.6379
Lipid Particles 0.0147 0.0036 0.0103 0.0084 0.0092 0.0124 0.038 0.0057 0.0089 0.0092 0.0203 0.0145 0.0213 0.0017 0.003 0.0274 0.0119 0.0046
Mitochondria 0.0674 0.0413 0.0847 0.0872 0.0422 0.062 0.0324 0.0207 0.0499 0.0146 0.0291 0.0431 0.0482 0.0235 0.0557 0.0524 0.0382 0.031
None 0.0096 0.0004 0.0054 0.0004 0.0002 0.0004 0.0032 0.0002 0.0007 0.0011 0.0005 0.0037 0.0086 0.0003 0.0008 0 0.0002 0.0003
Nuclear Periphery 0.0077 0.0007 0.0011 0.0005 0.0008 0.0035 0.0043 0.0002 0.0017 0.0006 0.0011 0.0044 0.0009 0.0002 0.0075 0.0001 0.0018 0.0036
Nucleolus 0.0027 0.0001 0.0005 0.0001 0.0001 0.0028 0.0015 0 0.0001 0.0031 0.0004 0.0003 0.006 0 0.0002 0.0001 0.0001 0.0001
Nucleus 0.0041 0.0002 0.0013 0.0003 0.0002 0.0019 0.0013 0.0001 0.0006 0.0022 0.0004 0.0012 0.0016 0.0001 0.0013 0 0.0002 0.0002
Peroxisomes 0.0087 0.0067 0.0144 0.0024 0.0118 0.008 0.0338 0.0086 0.0169 0.0106 0.0058 0.0132 0.0297 0.0026 0.0041 0.0194 0.0037 0.0051
Punctate Nuclear 0.0024 0.0002 0.0006 0.0001 0.0015 0.001 0.0047 0.0001 0.0006 0.0019 0.0024 0.003 0.0022 0.0001 0.0003 0.0001 0.0002 0.0014
Vacuole 0.004 0.003 0.0043 0.0139 0.0035 0.0027 0.0029 0.0024 0.0031 0.0076 0.0023 0.0035 0.0028 0.0023 0.0018 0.0017 0.0014 0.0013
Vacuole Periphery 0.0034 0.0024 0.0036 0.0052 0.005 0.0029 0.0028 0.002 0.0023 0.0032 0.0025 0.0038 0.0029 0.0021 0.0029 0.0029 0.0027 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.9815 14.0602 13.2422 15.924 14.1179 11.4874 14.8997 17.3448 14.6403 16.1129
Translational Efficiency 2.4279 1.5504 1.4321 1.3834 1.0565 1.748 1.3268 0.9483 1.256 1.2697

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
Localization
Cell Percentages Golgi (15%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cog8

Cog8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cog8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available