Standard name
Human Ortholog
Description Subunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0.06 0.07 0.05 0.08 0 0 0 0.06 0.05 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.96 0.92 0.85 0.87 – 0.71 0.71 0.65 0.67 0.99 0.99 0.99 0.98 0.98 0.97 0.96 0.95 0.95 0.96 0.93 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0.05 0 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.2 0.05 – 0.23 0.2 0.33 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 – 0.1 0.08 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 3 1 2 – 5 10 7 12 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 1 0 1 0 5 2 – 7 2 5 4 0 1 0 0 0 0 0 0 0 0 3 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 6 2 14 16 2 13 – 18 25 17 35 9 20 21 17 10 16 0 0 0 0 0 0
Cytoplasm 126 169 66 344 426 237 398 – 223 262 207 294 330 658 874 295 198 217 128 166 81 268 500 591
Endoplasmic Reticulum 2 1 0 1 9 4 14 – 4 20 2 21 3 1 2 8 3 14 0 1 1 2 14 8
Endosome 0 0 0 1 1 1 2 – 2 0 4 3 0 0 0 0 0 0 0 0 0 0 2 1
Golgi 0 0 0 0 3 0 1 – 0 0 0 0 0 1 1 0 0 1 0 0 0 1 1 1
Mitochondria 0 0 0 6 11 56 22 – 71 73 105 106 0 0 0 0 5 2 0 0 0 1 2 2
Nucleus 1 1 0 0 0 1 3 – 3 4 4 3 0 0 0 2 0 1 1 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 1 0 – 0 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 1 1 1 0 1 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 0 1 9 17 12 25 – 30 31 26 40 0 3 2 6 1 5 1 2 0 1 3 1
Unique Cell Count 128 172 67 357 461 279 455 314 369 317 439 332 663 880 302 203 223 135 175 86 280 536 619
Labelled Cell Count 131 179 69 376 486 320 482 363 431 378 523 342 685 900 328 217 256 135 175 86 280 536 619


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 8.8 7.9 8.9 9.3 6.7 8.4 – 7.9 7.7 7.8 7.9 10.0 9.7 10.1 11.8 12.1 12.4 9.0 9.2 9.2
Std Deviation (1e-4) 1.0 1.2 1.4 1.4 1.5 1.7 1.5 – 1.6 1.7 1.6 1.5 1.5 1.6 1.4 2.2 2.5 2.7 1.6 1.6 1.7
Intensity Change (Log2) – – – 0.17 0.24 -0.24 0.09 – -0.0 -0.04 -0.02 -0.0 0.34 0.3 0.36 0.58 0.62 0.66 0.2 0.22 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -1.9 -3.0 -2.7 – -4.8 -4.8 -5.4 -5.3 0.8 0.6 0.7 -0.4 -0.5 -0.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.0 0 – 4.3 4.0 5.5 4.5 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 2.2 0 0 2.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3366 3.4877 3.1247 2.7007 2.5077 3.1135 2.8545 5.0283 4.2209 3.8732 3.3739 4.4679 4.907 6.9875 5.7599 5.5668 5.5903 5.7579
Actin 0.0065 0.001 0.0012 0.0007 0.0002 0.0003 0.0388 0.0005 0.0151 0.0001 0.0017 0.0001 0.0088 0.0006 0.0038 0.0003 0.0003 0.0002
Bud 0.0002 0.0004 0.0014 0.0014 0.0001 0.0001 0.0008 0.0001 0.0006 0.0004 0.0006 0 0.0001 0.0001 0.0003 0.0002 0.0001 0.0001
Bud Neck 0.0003 0.0001 0.0003 0.0074 0.0002 0.0006 0.0014 0.0001 0.0011 0.0002 0.001 0.0004 0.0003 0.001 0.0002 0.0003 0.0002 0.0005
Bud Periphery 0.0001 0.0001 0.0009 0.0009 0 0 0.001 0 0.0003 0 0.0005 0 0.0001 0.0001 0.0002 0.0001 0 0
Bud Site 0.0004 0.0006 0.001 0.0035 0.0001 0 0.0016 0.0003 0.0008 0.0001 0.0016 0 0.0004 0.0138 0.0005 0.0002 0.0001 0
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0 0 0.0003 0.0001 0.0001 0 0.0001 0 0.0002 0.0012 0.0003 0 0 0
Cytoplasm 0.8911 0.9667 0.9374 0.8889 0.8932 0.9625 0.7977 0.9623 0.9292 0.9539 0.9228 0.9819 0.877 0.9512 0.9379 0.9411 0.9746 0.975
Cytoplasmic Foci 0.0094 0.0078 0.0094 0.0222 0.0217 0.0102 0.0287 0.0047 0.011 0.0122 0.0243 0.0043 0.0099 0.004 0.0098 0.0094 0.0062 0.0036
Eisosomes 0.0001 0 0 0 0 0 0.0004 0 0.0002 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0089 0.0015 0.0031 0.0034 0.0046 0.0031 0.0094 0.0049 0.0025 0.001 0.0015 0.0033 0.0181 0.0025 0.0045 0.0025 0.0015 0.0047
Endosome 0.0133 0.0063 0.0127 0.0221 0.056 0.0098 0.0339 0.008 0.0191 0.0181 0.0236 0.0048 0.0162 0.0024 0.0068 0.0206 0.0081 0.0061
Golgi 0.002 0.0006 0.0006 0.0014 0.0008 0.0003 0.008 0.0031 0.0026 0.0003 0.003 0.0002 0.0028 0.0003 0.0016 0.0012 0.0003 0.0008
Lipid Particles 0.002 0 0.0001 0.0002 0.0003 0.0001 0.009 0.0001 0.0027 0 0.0005 0 0.0031 0.0001 0.0004 0.0001 0 0
Mitochondria 0.0008 0.0002 0.0002 0.0006 0.0002 0.0002 0.0035 0.0002 0.0008 0.0001 0.0018 0.0002 0.0005 0.0001 0.0003 0.0004 0.0001 0.0019
None 0.0533 0.0121 0.026 0.0385 0.0104 0.0093 0.0314 0.0133 0.0058 0.0022 0.0086 0.0031 0.0509 0.0211 0.0278 0.0192 0.006 0.0056
Nuclear Periphery 0.0057 0.0003 0.0007 0.0012 0.0025 0.0007 0.0082 0.0005 0.0015 0.0006 0.0005 0.0003 0.0038 0.0002 0.0006 0.0006 0.0003 0.0002
Nucleolus 0.0001 0 0 0.0001 0 0 0.0005 0 0.0003 0 0.0001 0 0.0001 0 0 0 0 0
Nucleus 0.0014 0.0006 0.0009 0.0017 0.0016 0.001 0.003 0.0006 0.0013 0.0009 0.0011 0.0006 0.0012 0.0003 0.0006 0.0008 0.0004 0.0005
Peroxisomes 0.0001 0 0.0001 0.0005 0.0001 0.0001 0.0096 0 0.0015 0.0001 0.0018 0 0.002 0 0.0025 0.0001 0 0
Punctate Nuclear 0.0007 0.0001 0.0002 0.0008 0.0005 0.0003 0.0086 0.0001 0.0015 0.0002 0.0006 0 0.0012 0.0001 0.0004 0.0001 0.0001 0
Vacuole 0.0025 0.0011 0.0032 0.0035 0.0058 0.001 0.0029 0.001 0.0017 0.0081 0.0035 0.0006 0.003 0.0008 0.0013 0.0023 0.0014 0.0005
Vacuole Periphery 0.0006 0.0002 0.0005 0.0009 0.0014 0.0003 0.0012 0.0002 0.0005 0.0015 0.0008 0.0002 0.0005 0.0001 0.0002 0.0005 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.4898 28.0676 31.2411 43.521 30.5314 26.8974 26.8588 32.1514 26.0668 42.0891
Translational Efficiency 2.7663 4.4705 3.203 1.6739 3.4207 4.3748 3.6938 2.9261 3.5219 3.0532

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1384 721 1508 1376 1537 2550 2325 1210 2921 3271 3833 2586

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 890.25 902.49 1123.10 1140.06 854.81 989.86 1113.59 1181.86 871.60 970.60 1117.33 1159.62
Standard Deviation 112.92 138.36 134.72 183.50 106.70 126.52 144.76 217.33 111.11 134.20 140.97 201.13
Intensity Change Log 2 — 0.019700 0.335204 0.356827 — 0.211621 0.381542 0.467383 — 0.116902 0.358089 0.412042

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000485 0.000484 0.000237 0.000622 0.000237 0.000178 0.000117 0.000583 0.000354 0.000246 0.000164 0.000603
Bud Neck 0.003572 0.001627 0.001172 0.001441 0.002121 0.002676 0.000797 0.002913 0.002808 0.002445 0.000945 0.002130
Bud Site 0.020710 0.023243 0.007342 0.021098 0.013667 0.013843 0.005700 0.024459 0.017004 0.015915 0.006346 0.022671
Cell Periphery 0.000195 0.000137 0.000057 0.000116 0.000128 0.000066 0.000044 0.000165 0.000159 0.000081 0.000049 0.000139
Cytoplasm 0.568914* 0.716174* 0.790230* 0.835825* 0.684304* 0.760373* 0.846143* 0.793516* 0.629631* 0.750631* 0.824145* 0.816029*
Cytoplasmic Foci 0.288921* 0.157246 0.044707 0.036596 0.212416* 0.118473 0.030432 0.030130 0.248665* 0.127019 0.036048 0.033570
Eisosomes 0.000063 0.000017 0.000009 0.000005 0.000028 0.000015 0.000005 0.000008 0.000045 0.000016 0.000006 0.000006
Endoplasmic Reticulum 0.003523 0.002061 0.000999 0.000537 0.003110 0.000801 0.000215 0.000306 0.003306 0.001079 0.000523 0.000429
Endosome 0.020260 0.015478 0.004326 0.003706 0.011835 0.007568 0.001742 0.006346 0.015827 0.009311 0.002759 0.004942
Golgi 0.011752 0.006057 0.000422 0.003563 0.009935 0.005612 0.000299 0.005286 0.010796 0.005710 0.000347 0.004369
Lipid Particles 0.002914 0.002050 0.000939 0.001494 0.002188 0.000854 0.000403 0.001114 0.002532 0.001118 0.000614 0.001316
Mitochondria 0.003437 0.000865 0.000096 0.001191 0.001662 0.000901 0.000078 0.002338 0.002503 0.000893 0.000085 0.001728
Mitotic Spindle 0.002094 0.001236 0.003522 0.007168 0.001695 0.004759 0.002106 0.009916 0.001884 0.003982 0.002663 0.008454
None 0.000678 0.001316 0.000900 0.000351 0.001010 0.001788 0.001239 0.002626 0.000852 0.001684 0.001105 0.001415
Nuclear Periphery 0.000056 0.000118 0.000265 0.000126 0.000068 0.000045 0.000062 0.000198 0.000062 0.000061 0.000142 0.000160
Nuclear Periphery Foci 0.000681 0.001558 0.003570 0.002341 0.000656 0.000403 0.001226 0.001723 0.000668 0.000657 0.002148 0.002052
Nucleolus 0.000233 0.000454 0.000112 0.000125 0.000263 0.000235 0.000128 0.000434 0.000249 0.000283 0.000122 0.000270
Nucleus 0.009889 0.009066 0.031920 0.009445 0.010692 0.016168 0.028238 0.016368 0.010311 0.014603 0.029686 0.012684
Peroxisomes 0.001626 0.001831 0.000265 0.001028 0.000902 0.001223 0.000198 0.001678 0.001245 0.001357 0.000224 0.001332
Vacuole 0.054705 0.054538 0.108396 0.071739 0.038071 0.061789 0.080479 0.095094 0.045953 0.060191 0.091462 0.082667
Vacuole Periphery 0.005292 0.004445 0.000516 0.001481 0.005013 0.002230 0.000349 0.004800 0.005145 0.002718 0.000415 0.003034

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.00 2.11 -0.37 -0.30 -1.90 0.87 1.98 -2.69 -3.54 -4.37 1.75 3.20 -1.96 -3.24 -4.22
Bud Neck 6.00 7.50 7.31 0.74 -0.68 -1.77 5.88 -0.64 0.17 -2.62 1.47 9.94 2.40 1.32 -2.92
Bud Site -1.00 9.80 0.91 1.55 -5.84 -0.07 8.52 -3.39 -3.36 -7.87 1.06 13.29 -1.77 -2.46 -9.86
Cell Periphery 2.75 9.68 5.52 1.73 -5.33 9.37 13.26 -1.31 -5.61 -7.21 9.39 14.83 3.06 -4.90 -9.25
Cytoplasm -12.36 -19.78 -26.84 -8.41 -6.93 -9.78 -18.81 -8.98 -1.44 6.08 -19.14 -28.17 -24.38 -6.84 0.54
Cytoplasmic Foci 15.68 42.29 43.36 18.03 2.69 16.40 35.93 35.32 26.31 0.15 26.35 54.88 54.57 31.13 1.16
Eisosomes 10.96 14.98 16.25 5.32 7.17 7.31 13.86 11.71 8.44 -4.44 14.25 19.69 19.72 11.61 0.59
Endoplasmic Reticulum 2.40 5.25 6.16 4.06 2.06 5.02 6.67 6.55 3.63 -1.22 6.33 8.64 8.90 4.43 1.23
Endosome 2.72 16.04 16.26 7.98 1.88 5.48 14.88 7.21 3.47 -4.32 8.84 22.02 16.83 9.01 -3.16
Golgi 6.76 20.70 9.31 3.30 -2.69 6.47 17.57 6.04 2.04 -3.67 9.97 26.92 10.77 3.80 -4.50
Lipid Particles 2.25 8.61 3.90 1.27 -1.62 6.97 9.69 3.46 -3.17 -6.19 8.65 13.30 4.94 -1.60 -4.40
Mitochondria 2.34 3.26 2.24 0.03 -1.43 2.07 4.50 0.14 -1.17 -2.65 3.02 4.65 2.03 -0.73 -2.86
Mitotic Spindle 1.03 -2.14 -2.57 -3.21 -1.01 -2.60 -0.33 -2.79 -1.10 -2.64 -2.34 -1.58 -3.81 -1.91 -2.85
None -1.48 -0.68 1.22 3.01 8.11 -4.04 -0.79 -1.37 -0.20 -1.22 -4.01 -1.07 -0.66 1.74 -0.20
Nuclear Periphery -1.70 -7.52 -1.47 0.09 3.94 1.24 -0.94 -1.76 -2.18 -1.56 0.01 -6.84 -2.26 -2.27 0.84
Nuclear Periphery Foci -4.12 -8.58 -5.94 -2.31 3.16 1.65 -6.22 -6.24 -6.84 -2.93 -0.25 -10.34 -8.43 -7.67 0.46
Nucleolus -0.93 1.14 1.11 1.56 -0.24 0.34 1.53 -0.68 -1.14 -2.09 -0.41 1.85 0.24 0.76 -2.05
Nucleus 0.62 -17.15 -1.94 -2.29 17.06 -6.62 -20.69 -8.14 -2.70 9.08 -5.88 -26.43 -7.20 -0.88 19.23
Peroxisomes -0.48 10.95 2.70 1.97 -2.47 -2.19 10.19 -1.33 -0.23 -3.86 -0.73 14.92 0.84 1.19 -4.55
Vacuole -0.14 -15.93 -9.99 -7.97 6.60 -9.62 -16.76 -14.56 -10.25 -4.08 -6.86 -22.44 -17.85 -13.04 0.87
Vacuole Periphery 0.88 6.87 5.16 4.11 -1.59 4.81 8.30 1.81 -1.29 -3.73 5.04 10.67 4.91 1.27 -4.05
Standard name
Human Ortholog
Description Subunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Gim5

Gim5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gim5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available