Standard name
Human Ortholog
Description Single-stranded DNA endonuclease (with Rad1p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.21 0.12 0.12 0.18 0.23 0.34 0.29 – 0.19 0.16 0.12 0.18 0.3 0.29 0.27 0.15 0.3 0.26 0.1 0.12 0.17 0.15 0.11 0.16
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0.18 0.24 0 0.49 0.16 – 0.57 0.43 0.53 0.35 0 0 0 0.06 0 0 0 0 0 0 0 0.05
Nucleus 0.55 0.55 0.59 0.53 0.52 0.34 0.45 – 0.4 0.5 0.49 0.48 0.42 0.41 0.45 0.29 0.27 0.3 0.57 0.67 0.57 0.49 0.5 0.36
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.32 0.43 0.18 0.18 0.24 0.06 0.16 – 0.11 0.12 0.11 0.13 0.37 0.37 0.33 0.6 0.5 0.5 0.24 0.13 0.15 0.23 0.27 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1
Bud 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 1 1 0 3 7 11
Bud Neck 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 4
Bud Site 0 0 0 0 0 0 0 – 0 2 3 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 42 20 2 3 19 28 60 – 29 30 19 36 52 85 88 19 26 28 24 21 6 32 38 54
Endoplasmic Reticulum 1 2 0 0 0 0 0 – 0 0 0 0 0 0 0 10 3 5 0 0 0 0 0 4
Endosome 2 2 0 0 4 0 6 – 4 1 1 4 4 11 16 7 2 0 3 1 0 4 6 4
Golgi 1 0 0 1 0 1 0 – 0 0 0 0 0 1 0 0 3 2 2 0 0 0 1 2
Mitochondria 6 12 3 4 3 40 32 – 86 79 81 69 0 0 1 8 2 1 2 4 1 5 6 16
Nucleus 108 89 10 9 43 28 92 – 60 92 75 94 72 119 149 37 24 33 136 120 22 109 172 123
Nuclear Periphery 0 1 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 2 0 0 0 0 1 2
Nucleolus 0 0 0 0 0 0 0 – 0 0 1 2 0 0 0 2 0 0 1 0 0 2 2 4
Peroxisomes 0 0 0 0 0 0 1 – 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 2 – 1 2 0 2 0 1 2 1 0 1 4 1 1 6 8 14
Vac/Vac Membrane 64 69 3 3 20 5 33 – 17 23 17 25 63 109 108 76 44 54 58 24 5 51 92 90
Unique Cell Count 198 161 17 17 82 82 204 150 184 153 195 172 292 332 127 88 109 240 181 39 223 345 344
Labelled Cell Count 224 195 18 20 90 103 226 197 230 199 238 191 327 364 161 104 126 240 181 39 223 345 344


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.2 4.3 4.7 5.3 3.7 4.9 – 4.5 4.4 4.2 4.6 5.6 5.8 5.8 6.2 6.8 6.4 4.9 5.2 5.6
Std Deviation (1e-4) 0.7 1.2 0.5 1.7 0.9 1.3 2.2 – 2.4 1.5 1.3 2.3 1.5 1.4 1.3 1.6 1.7 1.8 1.1 1.6 1.7
Intensity Change (Log2) – – – 0.11 0.29 -0.25 0.16 – 0.04 0.01 -0.04 0.07 0.38 0.42 0.41 0.52 0.65 0.56 0.19 0.25 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.8 0 – 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus -0.3 -0.5 -1.9 -1.1 – -1.5 -0.7 -0.8 -0.8 -1.3 -1.5 -1.1 -2.5 -2.5 -2.3
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 1.6 1.6 1.3 3.3 2.5 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.603 -0.1351 -0.4313 -0.5543 -0.9142 -0.6077 0.7436 1.3223 1.1021 0.4218 0.5724 0.9542 2.3545 2.5362 2.3113 2.3747 2.2328 2.5601
Actin 0.0224 0.0004 0.0078 0.0003 0.0036 0.0006 0.0054 0.0001 0.0133 0.0001 0.0327 0.0031 0.0078 0.0001 0.0066 0.0002 0.0098 0.0041
Bud 0.0003 0.0089 0.0012 0 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0.0009 0.0009 0.0008 0.0001 0.001 0.0001 0.0004 0.0002
Bud Neck 0.0036 0.0005 0.0007 0.0006 0.0005 0.0025 0.0009 0.0003 0.0006 0.0007 0.0011 0.0031 0.0085 0.0005 0.0011 0.0003 0.0008 0.0097
Bud Periphery 0.0005 0.0013 0.0003 0 0.0001 0.0001 0.0004 0.0001 0.0005 0 0.0019 0.0009 0.0013 0 0.0022 0 0.0005 0.0002
Bud Site 0.0029 0.0023 0.0043 0.0002 0.0002 0.0002 0.0013 0.0008 0.0013 0.0004 0.0014 0.0012 0.0078 0.0006 0.0023 0.0003 0.0007 0.0019
Cell Periphery 0.0002 0.0001 0.0001 0 0 0 0.0005 0.0001 0.0002 0 0.0001 0.0001 0.0005 0 0.0003 0 0 0
Cytoplasm 0.1048 0.1497 0.0924 0.17 0.0774 0.0951 0.0596 0.1966 0.1205 0.0488 0.1184 0.0971 0.1013 0.1293 0.0771 0.125 0.0817 0.1193
Cytoplasmic Foci 0.0244 0.0026 0.0126 0.0022 0.0048 0.0117 0.0239 0.001 0.0098 0.0006 0.0124 0.006 0.0094 0.0033 0.0079 0.0008 0.0031 0.0045
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0004 0 0.0001 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0066 0.0088 0.0041 0.0049 0.0026 0.0027 0.013 0.0055 0.0026 0.0006 0.0035 0.0043 0.0047 0.003 0.0023 0.0018 0.0035 0.0025
Endosome 0.0215 0.012 0.0067 0.0051 0.0273 0.0079 0.0186 0.0022 0.0109 0.0009 0.03 0.0182 0.0279 0.0045 0.0047 0.001 0.0085 0.0042
Golgi 0.0047 0.0001 0.0035 0.0001 0.0182 0.003 0.0125 0 0.006 0 0.0099 0.0046 0.0057 0.0001 0.0028 0 0.0031 0.0013
Lipid Particles 0.0078 0.0005 0.0062 0.0002 0.0223 0.0013 0.0065 0.0001 0.007 0 0.013 0.0048 0.0092 0.0003 0.0049 0 0.0015 0.0011
Mitochondria 0.0048 0.0012 0.0079 0.0005 0.0025 0.0006 0.0139 0.0004 0.0188 0.0005 0.0978 0.0027 0.0103 0.0003 0.0092 0.0006 0.0046 0.0007
None 0.0768 0.0986 0.1242 0.0917 0.0461 0.0542 0.0058 0.041 0.0738 0.005 0.0385 0.051 0.0516 0.0405 0.1181 0.0556 0.1043 0.1173
Nuclear Periphery 0.034 0.046 0.0431 0.0267 0.0153 0.0226 0.0114 0.0202 0.0161 0.0091 0.0163 0.0136 0.0311 0.0207 0.0172 0.0151 0.024 0.0078
Nucleolus 0.0024 0.0039 0.0029 0.0023 0.0022 0.0037 0.0014 0.0017 0.0025 0.0042 0.0036 0.002 0.0043 0.0022 0.0042 0.0018 0.0051 0.0042
Nucleus 0.64 0.6176 0.6407 0.6546 0.7613 0.7523 0.8053 0.7188 0.6942 0.9177 0.5835 0.7571 0.6902 0.7782 0.7072 0.7923 0.7202 0.6977
Peroxisomes 0.016 0.0001 0.0072 0.0001 0.0017 0.0001 0.0041 0 0.0033 0 0.0148 0.0013 0.0034 0.0001 0.0046 0 0.0112 0.0017
Punctate Nuclear 0.022 0.0395 0.0316 0.0378 0.0117 0.0393 0.0124 0.0075 0.0103 0.0105 0.0163 0.0231 0.0148 0.0135 0.0233 0.0035 0.0123 0.0198
Vacuole 0.0031 0.0047 0.0018 0.0021 0.0014 0.0015 0.0021 0.0031 0.0063 0.0006 0.0021 0.0031 0.007 0.0024 0.0021 0.0011 0.0029 0.0015
Vacuole Periphery 0.001 0.0011 0.0007 0.0004 0.0006 0.0003 0.0007 0.0004 0.0017 0.0002 0.0012 0.0017 0.002 0.0003 0.0008 0.0003 0.0015 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.3578 4.6748 6.9378 6.8123 3.3901 5.2265 3.1511 6.912 4.9957 5.1897
Translational Efficiency 1.6673 1.6195 1.0705 1.4794 2.636 2.0294 2.8472 1.8496 2.1536 2.1692

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Single-stranded DNA endonuclease (with Rad1p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
Localization
Cell Percentages nucleus (42%), cytoplasm (3%), mixed (49%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rad10

Rad10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available