ORF
Human Ortholog
Description Putative protein with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.98 0.97 0.93 0.88 0.83 0.76 0.72 0.77 0.73 0.99 1.0 0.99 0.96 0.96 0.97 0.97 0.96 0.85 0.92 0.94 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.05 0.24 0.32 0.35 0.44 0.48 0.45 0.5 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud 0 1 5 3 2 2 1 10 7 3 6 0 2 0 0 0 0 0 0 0 1 1 0 4
Bud Neck 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 1 1 0 5 5 3 1 2 0 2 2 10 3 2 0 0 0 0 0 0
Cytoplasm 180 144 157 172 299 254 321 415 278 274 165 109 170 304 303 199 74 85 174 138 149 134 283 246
Endoplasmic Reticulum 1 1 0 0 0 0 3 0 1 2 0 0 1 1 0 8 2 7 0 0 4 0 2 2
Endosome 0 0 0 0 1 1 1 1 0 0 0 0 0 0 1 2 0 1 1 0 1 1 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 1
Mitochondria 6 2 4 12 16 65 117 178 162 183 96 75 0 0 0 6 4 1 0 1 2 1 3 1
Nucleus 0 0 0 0 0 1 1 2 1 1 1 4 1 0 1 2 0 1 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 1 1 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 1 0 2 1 0 0 0 1 0 0 0 0 0 0 1 2
Vac/Vac Membrane 0 0 1 0 1 2 4 4 4 4 0 0 0 0 0 2 3 0 1 2 12 2 1 5
Unique Cell Count 183 145 159 175 308 272 363 503 368 378 213 149 171 305 305 207 77 88 179 144 177 146 302 276
Labelled Cell Count 188 149 167 187 322 328 450 618 463 471 272 191 174 307 307 232 87 97 179 144 177 146 302 276


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.6 4.9 4.5 5.0 4.0 3.6 3.6 3.5 3.2 3.2 3.3 6.1 6.0 5.9 7.2 7.8 9.0 6.4 6.1 6.7
Std Deviation (1e-4) 0.7 0.8 0.8 0.8 1.8 1.3 1.4 1.1 1.0 1.1 0.7 1.5 0.9 1.1 1.2 1.2 1.5 1.5 1.7 1.3 1.3
Intensity Change (Log2) – – – -0.12 0.03 -0.28 -0.45 -0.42 -0.48 -0.61 -0.61 -0.58 0.31 0.3 0.28 0.55 0.68 0.88 0.4 0.32 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -1.1 -2.6 -3.9 -5.2 -6.4 -7.0 -6.0 -6.5 0.6 1.2 0.7 -1.5 -1.3 -1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.4 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.015 0.001 0.0212 0.0012 0.0012 0.0051 0.064 0.0032 0.0124 0.0011 0.0217 0.0161 0 0.0008 0.0222 0.0001 0.0006 0.0003
Bud 0.0052 0.0002 0.0005 0.0002 0.0001 0.0003 0.0003 0.0002 0.0007 0.0004 0.0013 0.0011 0 0.0001 0.0002 0.0001 0.0001 0
Bud Neck 0.001 0.0004 0.0022 0.0003 0.0003 0.001 0.0002 0.0002 0.0008 0.0002 0.0005 0.0006 0 0.0001 0.0098 0.0002 0.0003 0.0005
Bud Periphery 0.0131 0.0001 0.0006 0.0001 0 0.0002 0.0004 0.0002 0.0011 0.0002 0.0019 0.0023 0 0 0.0002 0 0 0
Bud Site 0.0026 0.006 0.0056 0.0004 0.0001 0.0003 0.0006 0.0011 0.0069 0.0002 0.0031 0.0001 0 0.0005 0.0009 0.0001 0.0001 0
Cell Periphery 0.0003 0.0005 0.0004 0.0001 0.0001 0.0001 0.0003 0.0003 0.0002 0.0001 0.0003 0.0001 0 0.0003 0.0002 0 0 0
Cytoplasm 0.5462 0.7904 0.7913 0.8211 0.6515 0.8084 0.4843 0.6495 0.5496 0.713 0.5218 0.7832 0.9398 0.7554 0.6613 0.956 0.9852 0.928
Cytoplasmic Foci 0.0267 0.0059 0.0093 0.0122 0.0088 0.0094 0.0111 0.0305 0.0392 0.0143 0.0451 0.0038 0.0012 0.0018 0.0182 0.0022 0.0023 0.0044
Eisosomes 0.0001 0.0002 0.0002 0 0 0.0001 0.0006 0.0001 0.0002 0 0.0002 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0039 0.0041 0.0065 0.0027 0.0017 0.0057 0.0064 0.0032 0.0049 0.0056 0.0049 0.0021 0.0011 0.0136 0.0053 0.0007 0.0006 0.0018
Endosome 0.0261 0.0014 0.0032 0.0144 0.0016 0.0058 0.0271 0.0155 0.0247 0.012 0.0471 0.0015 0.0002 0.0006 0.0141 0.0005 0.0004 0.0013
Golgi 0.0035 0.0003 0.0011 0.0019 0.0003 0.003 0.0078 0.0052 0.0149 0.0038 0.0201 0.0002 0 0.0002 0.0431 0 0.0001 0.0001
Lipid Particles 0.0044 0.0003 0.0011 0.0098 0.0004 0.002 0.0344 0.0039 0.0069 0.0001 0.0078 0.0001 0 0.0004 0.008 0 0 0.0001
Mitochondria 0.003 0.0001 0.0003 0.001 0.0002 0.003 0.0015 0.0009 0.0164 0.0011 0.0257 0.0002 0 0 0.0171 0.0001 0 0.0001
None 0.3141 0.1852 0.1481 0.124 0.3313 0.1508 0.3442 0.2818 0.2957 0.2428 0.26 0.187 0.0574 0.2241 0.1475 0.0367 0.0096 0.0625
Nuclear Periphery 0.0019 0.0006 0.0015 0.0013 0.0003 0.0006 0.0046 0.0006 0.001 0.0012 0.0009 0.0003 0.0001 0.0009 0.0005 0.0004 0.0001 0.0001
Nucleolus 0.001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0003 0.0001 0.0002 0 0.0001 0 0 0 0.0001 0 0 0
Nucleus 0.0022 0.0011 0.0012 0.0013 0.0007 0.001 0.0013 0.0007 0.001 0.0013 0.0007 0.0005 0.0001 0.0006 0.0005 0.0019 0.0004 0.0004
Peroxisomes 0.001 0.0001 0.0028 0.0027 0.0003 0.001 0.0012 0.0014 0.0189 0.0002 0.032 0 0 0 0.0494 0 0 0.0001
Punctate Nuclear 0.0205 0.0011 0.0011 0.0028 0.0005 0.001 0.0012 0.0005 0.0013 0.0005 0.0009 0.0001 0 0.0002 0.0003 0.0003 0.0001 0.0001
Vacuole 0.0062 0.001 0.0017 0.002 0.0005 0.0008 0.0042 0.0009 0.0025 0.0015 0.0034 0.0006 0.0001 0.0004 0.0006 0.0004 0.0001 0.0002
Vacuole Periphery 0.0019 0.0001 0.0002 0.0004 0.0001 0.0002 0.0039 0.0002 0.0004 0.0004 0.0007 0.0001 0 0 0.0002 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.9773 11.2514 12.0597 14.7001 17.2254 16.0737 9.7963 7.6782 10.9106 17.8069
Translational Efficiency 0.7265 0.8012 0.6655 0.5397 0.5833 0.8127 0.8721 0.6374 0.5581 0.6303

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
515 1393 397 1234 934 1817 74 298 1449 3210 471 1532

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 742.10 838.31 900.59 894.79 787.53 815.00 1029.40 986.64 771.38 825.12 920.83 912.66
Standard Deviation 85.71 115.94 150.24 114.64 91.19 109.05 113.07 111.18 91.89 112.69 152.42 119.64
Intensity Change Log 2 — 0.175870 0.279257 0.269936 — 0.049465 0.386397 0.325189 — 0.112175 0.335411 0.298647

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000078 0.000243 0.000719 0.000682 0.000201 0.000238 0.000288 0.001032 0.000157 0.000240 0.000651 0.000750
Bud Neck 0.006269 0.007786 0.002282 0.007294 0.008480 0.011840 0.001824 0.004175 0.007694 0.010080 0.002210 0.006687
Bud Site 0.005809 0.014697 0.012514 0.050014 0.007551 0.018192 0.003899 0.023897 0.006932 0.016675 0.011160 0.044934
Cell Periphery 0.000134 0.000072 0.000181 0.000185 0.000143 0.000069 0.000145 0.000150 0.000140 0.000070 0.000175 0.000178
Cytoplasm 0.629915* 0.650562* 0.538262* 0.551391* 0.564990* 0.632857* 0.460921* 0.501675* 0.588065* 0.640540* 0.526111* 0.541720*
Cytoplasmic Foci 0.189450* 0.154430 0.023152 0.028829 0.234244* 0.157795 0.019140 0.027616 0.218324* 0.156335 0.022521 0.028593
Eisosomes 0.000099 0.000040 0.000046 0.000034 0.000121 0.000048 0.000058 0.000044 0.000113 0.000045 0.000048 0.000036
Endoplasmic Reticulum 0.000453 0.000580 0.004474 0.001234 0.001221 0.000265 0.002889 0.003173 0.000948 0.000401 0.004225 0.001611
Endosome 0.013327 0.016815 0.003463 0.014199 0.019234 0.019109 0.004376 0.012039 0.017134 0.018113 0.003606 0.013779
Golgi 0.001595 0.002682 0.000227 0.009223 0.002480 0.005758 0.000113 0.005731 0.002165 0.004423 0.000209 0.008543
Lipid Particles 0.003109 0.001480 0.001610 0.001272 0.004535 0.001314 0.001633 0.001210 0.004028 0.001386 0.001614 0.001260
Mitochondria 0.000641 0.002029 0.000349 0.006176 0.001563 0.001934 0.000181 0.000469 0.001236 0.001976 0.000323 0.005066
Mitotic Spindle 0.002253 0.004120 0.003471 0.062395 0.001867 0.012327 0.001262 0.039088 0.002005 0.008766 0.003124 0.057861
None 0.003677 0.001781 0.004494 0.005054 0.001019 0.001183 0.004558 0.014527 0.001964 0.001442 0.004504 0.006897
Nuclear Periphery 0.000170 0.000128 0.000949 0.000916 0.000188 0.000093 0.001277 0.000523 0.000181 0.000108 0.001000 0.000839
Nuclear Periphery Foci 0.001091 0.000748 0.005205 0.001473 0.000954 0.000332 0.004837 0.001517 0.001003 0.000513 0.005147 0.001482
Nucleolus 0.000438 0.001012 0.000290 0.000765 0.000566 0.000482 0.000206 0.000093 0.000520 0.000712 0.000277 0.000634
Nucleus 0.067114 0.034054 0.286988* 0.075095 0.050072 0.030865 0.189925* 0.069606 0.056129 0.032249 0.271738* 0.074028
Peroxisomes 0.001175 0.003986 0.000720 0.002645 0.002593 0.004481 0.000138 0.001426 0.002089 0.004266 0.000628 0.002408
Vacuole 0.072630 0.101141 0.110386 0.175422 0.096434 0.099230 0.302170* 0.291693* 0.087974 0.100059 0.140518 0.198039
Vacuole Periphery 0.000572 0.001614 0.000218 0.005702 0.001546 0.001589 0.000161 0.000316 0.001200 0.001600 0.000209 0.004655

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.85 -6.24 -7.40 -2.58 3.76 -0.77 -2.44 -2.46 -2.24 -0.91 -2.42 -5.86 -5.46 -3.74 3.37
Bud Neck -1.04 2.40 0.78 2.60 -2.47 -2.97 7.56 4.26 6.91 -1.45 -2.69 6.56 3.21 6.32 -2.71
Bud Site -4.85 -1.66 -5.67 -2.51 -2.46 -7.67 1.95 -2.00 1.32 -2.62 -8.96 -1.34 -6.13 -1.51 -2.76
Cell Periphery 6.64 -4.18 2.26 -1.53 5.27 7.19 -0.48 3.59 -1.19 3.04 9.58 -4.01 3.38 -1.82 5.89
Cytoplasm -1.43 11.02 14.40 18.27 0.24 -6.45 7.12 10.49 14.54 -0.42 -6.36 11.27 16.04 23.25 0.28
Cytoplasmic Foci 4.61 23.99 25.25 29.02 1.57 10.65 32.64 35.69 32.80 0.38 11.56 40.34 43.09 43.35 1.69
Eisosomes 7.97 4.22 8.15 0.14 5.46 10.48 4.07 10.81 1.04 3.45 13.24 7.51 14.23 2.15 6.28
Endoplasmic Reticulum -1.09 -11.21 -8.01 -6.09 9.65 4.89 -3.35 -0.83 -3.81 2.80 4.11 -10.50 -2.23 -9.56 9.96
Endosome -1.82 6.74 5.59 8.78 -0.61 0.24 10.46 9.65 10.19 0.80 -0.48 12.47 10.56 12.59 -0.32
Golgi -1.43 2.19 -0.81 0.31 -3.08 -3.56 7.37 2.68 4.87 -0.94 -3.75 6.42 0.21 3.04 -3.20
Lipid Particles 2.68 1.61 3.29 2.20 5.85 6.12 4.65 6.15 0.23 2.22 6.56 4.67 7.17 2.14 6.23
Mitochondria -1.79 1.02 -1.55 0.05 -2.01 -0.52 2.56 2.32 4.62 -2.29 -1.36 2.52 -0.50 0.45 -2.04
Mitotic Spindle -1.12 -0.66 -7.28 -6.89 -7.15 -4.61 -1.15 -2.62 -0.25 -1.84 -4.54 -1.25 -8.03 -5.55 -7.37
None 1.16 0.07 -0.34 -4.08 -1.06 -0.97 -2.82 -5.59 -5.45 -3.11 0.90 -2.32 -3.81 -6.73 -2.34
Nuclear Periphery 1.44 -14.41 -16.85 -17.63 -1.39 6.21 -9.74 -12.23 -13.25 0.89 5.40 -16.76 -19.78 -20.98 -1.06
Nuclear Periphery Foci 1.19 -9.41 -0.46 -4.82 10.35 7.51 -5.96 -3.59 -7.97 4.87 4.16 -11.54 -2.21 -10.22 11.12
Nucleolus -1.20 2.74 0.10 1.20 -1.11 0.50 2.22 2.04 3.62 -0.58 -0.82 2.10 1.02 1.44 -1.12
Nucleus 8.15 -19.69 -10.38 -20.53 14.68 8.71 -5.79 -7.26 -9.67 3.16 11.68 -21.55 -16.02 -23.60 14.65
Peroxisomes -4.80 1.61 0.93 5.26 -0.78 -2.86 4.61 3.66 9.01 -1.55 -4.58 3.68 3.22 9.30 -0.91
Vacuole -6.17 -9.71 -27.91 -24.95 -10.60 -1.13 -8.75 -18.65 -18.47 -1.35 -4.34 -11.33 -32.51 -31.17 -9.95
Vacuole Periphery -4.76 3.77 -1.98 0.06 -2.74 -0.04 4.36 4.42 6.78 0.68 -1.43 4.70 -0.13 0.74 -2.72
ORF
Human Ortholog
Description Putative protein with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10
Localization
Cell Percentages cytoplasm (87%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YML096W

YML096W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yml096w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available