ORF
Human Ortholog
Description Putative protein with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.98 0.97 0.93 0.88 0.83 0.76 0.72 0.77 0.73 0.99 1.0 0.99 0.96 0.96 0.97 0.97 0.96 0.85 0.92 0.94 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.05 0.24 0.32 0.35 0.44 0.48 0.45 0.5 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud 0 1 5 3 2 2 1 10 7 3 6 0 2 0 0 0 0 0 0 0 1 1 0 4
Bud Neck 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 1 1 0 5 5 3 1 2 0 2 2 10 3 2 0 0 0 0 0 0
Cytoplasm 180 144 157 172 299 254 321 415 278 274 165 109 170 304 303 199 74 85 174 138 149 134 283 246
Endoplasmic Reticulum 1 1 0 0 0 0 3 0 1 2 0 0 1 1 0 8 2 7 0 0 4 0 2 2
Endosome 0 0 0 0 1 1 1 1 0 0 0 0 0 0 1 2 0 1 1 0 1 1 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 1
Mitochondria 6 2 4 12 16 65 117 178 162 183 96 75 0 0 0 6 4 1 0 1 2 1 3 1
Nucleus 0 0 0 0 0 1 1 2 1 1 1 4 1 0 1 2 0 1 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 1 1 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 1 0 2 1 0 0 0 1 0 0 0 0 0 0 1 2
Vac/Vac Membrane 0 0 1 0 1 2 4 4 4 4 0 0 0 0 0 2 3 0 1 2 12 2 1 5
Unique Cell Count 183 145 159 175 308 272 363 503 368 378 213 149 171 305 305 207 77 88 179 144 177 146 302 276
Labelled Cell Count 188 149 167 187 322 328 450 618 463 471 272 191 174 307 307 232 87 97 179 144 177 146 302 276


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.6 4.9 4.5 5.0 4.0 3.6 3.6 3.5 3.2 3.2 3.3 6.1 6.0 5.9 7.2 7.8 9.0 6.4 6.1 6.7
Std Deviation (1e-4) 0.7 0.8 0.8 0.8 1.8 1.3 1.4 1.1 1.0 1.1 0.7 1.5 0.9 1.1 1.2 1.2 1.5 1.5 1.7 1.3 1.3
Intensity Change (Log2) – – – -0.12 0.03 -0.28 -0.45 -0.42 -0.48 -0.61 -0.61 -0.58 0.31 0.3 0.28 0.55 0.68 0.88 0.4 0.32 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -1.1 -2.6 -3.9 -5.2 -6.4 -7.0 -6.0 -6.5 0.6 1.2 0.7 -1.5 -1.3 -1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.4 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.015 0.001 0.0212 0.0012 0.0012 0.0051 0.064 0.0032 0.0124 0.0011 0.0217 0.0161 0 0.0008 0.0222 0.0001 0.0006 0.0003
Bud 0.0052 0.0002 0.0005 0.0002 0.0001 0.0003 0.0003 0.0002 0.0007 0.0004 0.0013 0.0011 0 0.0001 0.0002 0.0001 0.0001 0
Bud Neck 0.001 0.0004 0.0022 0.0003 0.0003 0.001 0.0002 0.0002 0.0008 0.0002 0.0005 0.0006 0 0.0001 0.0098 0.0002 0.0003 0.0005
Bud Periphery 0.0131 0.0001 0.0006 0.0001 0 0.0002 0.0004 0.0002 0.0011 0.0002 0.0019 0.0023 0 0 0.0002 0 0 0
Bud Site 0.0026 0.006 0.0056 0.0004 0.0001 0.0003 0.0006 0.0011 0.0069 0.0002 0.0031 0.0001 0 0.0005 0.0009 0.0001 0.0001 0
Cell Periphery 0.0003 0.0005 0.0004 0.0001 0.0001 0.0001 0.0003 0.0003 0.0002 0.0001 0.0003 0.0001 0 0.0003 0.0002 0 0 0
Cytoplasm 0.5462 0.7904 0.7913 0.8211 0.6515 0.8084 0.4843 0.6495 0.5496 0.713 0.5218 0.7832 0.9398 0.7554 0.6613 0.956 0.9852 0.928
Cytoplasmic Foci 0.0267 0.0059 0.0093 0.0122 0.0088 0.0094 0.0111 0.0305 0.0392 0.0143 0.0451 0.0038 0.0012 0.0018 0.0182 0.0022 0.0023 0.0044
Eisosomes 0.0001 0.0002 0.0002 0 0 0.0001 0.0006 0.0001 0.0002 0 0.0002 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0039 0.0041 0.0065 0.0027 0.0017 0.0057 0.0064 0.0032 0.0049 0.0056 0.0049 0.0021 0.0011 0.0136 0.0053 0.0007 0.0006 0.0018
Endosome 0.0261 0.0014 0.0032 0.0144 0.0016 0.0058 0.0271 0.0155 0.0247 0.012 0.0471 0.0015 0.0002 0.0006 0.0141 0.0005 0.0004 0.0013
Golgi 0.0035 0.0003 0.0011 0.0019 0.0003 0.003 0.0078 0.0052 0.0149 0.0038 0.0201 0.0002 0 0.0002 0.0431 0 0.0001 0.0001
Lipid Particles 0.0044 0.0003 0.0011 0.0098 0.0004 0.002 0.0344 0.0039 0.0069 0.0001 0.0078 0.0001 0 0.0004 0.008 0 0 0.0001
Mitochondria 0.003 0.0001 0.0003 0.001 0.0002 0.003 0.0015 0.0009 0.0164 0.0011 0.0257 0.0002 0 0 0.0171 0.0001 0 0.0001
None 0.3141 0.1852 0.1481 0.124 0.3313 0.1508 0.3442 0.2818 0.2957 0.2428 0.26 0.187 0.0574 0.2241 0.1475 0.0367 0.0096 0.0625
Nuclear Periphery 0.0019 0.0006 0.0015 0.0013 0.0003 0.0006 0.0046 0.0006 0.001 0.0012 0.0009 0.0003 0.0001 0.0009 0.0005 0.0004 0.0001 0.0001
Nucleolus 0.001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0003 0.0001 0.0002 0 0.0001 0 0 0 0.0001 0 0 0
Nucleus 0.0022 0.0011 0.0012 0.0013 0.0007 0.001 0.0013 0.0007 0.001 0.0013 0.0007 0.0005 0.0001 0.0006 0.0005 0.0019 0.0004 0.0004
Peroxisomes 0.001 0.0001 0.0028 0.0027 0.0003 0.001 0.0012 0.0014 0.0189 0.0002 0.032 0 0 0 0.0494 0 0 0.0001
Punctate Nuclear 0.0205 0.0011 0.0011 0.0028 0.0005 0.001 0.0012 0.0005 0.0013 0.0005 0.0009 0.0001 0 0.0002 0.0003 0.0003 0.0001 0.0001
Vacuole 0.0062 0.001 0.0017 0.002 0.0005 0.0008 0.0042 0.0009 0.0025 0.0015 0.0034 0.0006 0.0001 0.0004 0.0006 0.0004 0.0001 0.0002
Vacuole Periphery 0.0019 0.0001 0.0002 0.0004 0.0001 0.0002 0.0039 0.0002 0.0004 0.0004 0.0007 0.0001 0 0 0.0002 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.9773 11.2514 12.0597 14.7001 17.2254 16.0737 9.7963 7.6782 10.9106 17.8069
Translational Efficiency 0.7265 0.8012 0.6655 0.5397 0.5833 0.8127 0.8721 0.6374 0.5581 0.6303

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
515 1393 397 1234 934 1817 74 298 1449 3210 471 1532

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 742.10 838.31 900.59 894.79 787.53 815.00 1029.40 986.64 771.38 825.12 920.83 912.66
Standard Deviation 85.71 115.94 150.24 114.64 91.19 109.05 113.07 111.18 91.89 112.69 152.42 119.64
Intensity Change Log 2 — 0.175870 0.279257 0.269936 — 0.049465 0.386397 0.325189 — 0.112175 0.335411 0.298647

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000078 0.000243 0.000719 0.000682 0.000201 0.000238 0.000288 0.001032 0.000157 0.000240 0.000651 0.000750
Bud Neck 0.006269 0.007786 0.002282 0.007294 0.008480 0.011840 0.001824 0.004175 0.007694 0.010080 0.002210 0.006687
Bud Site 0.005809 0.014697 0.012514 0.050014 0.007551 0.018192 0.003899 0.023897 0.006932 0.016675 0.011160 0.044934
Cell Periphery 0.000134 0.000072 0.000181 0.000185 0.000143 0.000069 0.000145 0.000150 0.000140 0.000070 0.000175 0.000178
Cytoplasm 0.629915* 0.650562* 0.538262* 0.551391* 0.564990* 0.632857* 0.460921* 0.501675* 0.588065* 0.640540* 0.526111* 0.541720*
Cytoplasmic Foci 0.189450* 0.154430 0.023152 0.028829 0.234244* 0.157795 0.019140 0.027616 0.218324* 0.156335 0.022521 0.028593
Eisosomes 0.000099 0.000040 0.000046 0.000034 0.000121 0.000048 0.000058 0.000044 0.000113 0.000045 0.000048 0.000036
Endoplasmic Reticulum 0.000453 0.000580 0.004474 0.001234 0.001221 0.000265 0.002889 0.003173 0.000948 0.000401 0.004225 0.001611
Endosome 0.013327 0.016815 0.003463 0.014199 0.019234 0.019109 0.004376 0.012039 0.017134 0.018113 0.003606 0.013779
Golgi 0.001595 0.002682 0.000227 0.009223 0.002480 0.005758 0.000113 0.005731 0.002165 0.004423 0.000209 0.008543
Lipid Particles 0.003109 0.001480 0.001610 0.001272 0.004535 0.001314 0.001633 0.001210 0.004028 0.001386 0.001614 0.001260
Mitochondria 0.000641 0.002029 0.000349 0.006176 0.001563 0.001934 0.000181 0.000469 0.001236 0.001976 0.000323 0.005066
Mitotic Spindle 0.002253 0.004120 0.003471 0.062395 0.001867 0.012327 0.001262 0.039088 0.002005 0.008766 0.003124 0.057861
None 0.003677 0.001781 0.004494 0.005054 0.001019 0.001183 0.004558 0.014527 0.001964 0.001442 0.004504 0.006897
Nuclear Periphery 0.000170 0.000128 0.000949 0.000916 0.000188 0.000093 0.001277 0.000523 0.000181 0.000108 0.001000 0.000839
Nuclear Periphery Foci 0.001091 0.000748 0.005205 0.001473 0.000954 0.000332 0.004837 0.001517 0.001003 0.000513 0.005147 0.001482
Nucleolus 0.000438 0.001012 0.000290 0.000765 0.000566 0.000482 0.000206 0.000093 0.000520 0.000712 0.000277 0.000634
Nucleus 0.067114 0.034054 0.286988* 0.075095 0.050072 0.030865 0.189925* 0.069606 0.056129 0.032249 0.271738* 0.074028
Peroxisomes 0.001175 0.003986 0.000720 0.002645 0.002593 0.004481 0.000138 0.001426 0.002089 0.004266 0.000628 0.002408
Vacuole 0.072630 0.101141 0.110386 0.175422 0.096434 0.099230 0.302170* 0.291693* 0.087974 0.100059 0.140518 0.198039
Vacuole Periphery 0.000572 0.001614 0.000218 0.005702 0.001546 0.001589 0.000161 0.000316 0.001200 0.001600 0.000209 0.004655

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.85 -6.24 -7.40 -2.58 3.76 -0.77 -2.44 -2.46 -2.24 -0.91 -2.42 -5.86 -5.46 -3.74 3.37
Bud Neck -1.04 2.40 0.78 2.60 -2.47 -2.97 7.56 4.26 6.91 -1.45 -2.69 6.56 3.21 6.32 -2.71
Bud Site -4.85 -1.66 -5.67 -2.51 -2.46 -7.67 1.95 -2.00 1.32 -2.62 -8.96 -1.34 -6.13 -1.51 -2.76
Cell Periphery 6.64 -4.18 2.26 -1.53 5.27 7.19 -0.48 3.59 -1.19 3.04 9.58 -4.01 3.38 -1.82 5.89
Cytoplasm -1.43 11.02 14.40 18.27 0.24 -6.45 7.12 10.49 14.54 -0.42 -6.36 11.27 16.04 23.25 0.28
Cytoplasmic Foci 4.61 23.99 25.25 29.02 1.57 10.65 32.64 35.69 32.80 0.38 11.56 40.34 43.09 43.35 1.69
Eisosomes 7.97 4.22 8.15 0.14 5.46 10.48 4.07 10.81 1.04 3.45 13.24 7.51 14.23 2.15 6.28
Endoplasmic Reticulum -1.09 -11.21 -8.01 -6.09 9.65 4.89 -3.35 -0.83 -3.81 2.80 4.11 -10.50 -2.23 -9.56 9.96
Endosome -1.82 6.74 5.59 8.78 -0.61 0.24 10.46 9.65 10.19 0.80 -0.48 12.47 10.56 12.59 -0.32
Golgi -1.43 2.19 -0.81 0.31 -3.08 -3.56 7.37 2.68 4.87 -0.94 -3.75 6.42 0.21 3.04 -3.20
Lipid Particles 2.68 1.61 3.29 2.20 5.85 6.12 4.65 6.15 0.23 2.22 6.56 4.67 7.17 2.14 6.23
Mitochondria -1.79 1.02 -1.55 0.05 -2.01 -0.52 2.56 2.32 4.62 -2.29 -1.36 2.52 -0.50 0.45 -2.04
Mitotic Spindle -1.12 -0.66 -7.28 -6.89 -7.15 -4.61 -1.15 -2.62 -0.25 -1.84 -4.54 -1.25 -8.03 -5.55 -7.37
None 1.16 0.07 -0.34 -4.08 -1.06 -0.97 -2.82 -5.59 -5.45 -3.11 0.90 -2.32 -3.81 -6.73 -2.34
Nuclear Periphery 1.44 -14.41 -16.85 -17.63 -1.39 6.21 -9.74 -12.23 -13.25 0.89 5.40 -16.76 -19.78 -20.98 -1.06
Nuclear Periphery Foci 1.19 -9.41 -0.46 -4.82 10.35 7.51 -5.96 -3.59 -7.97 4.87 4.16 -11.54 -2.21 -10.22 11.12
Nucleolus -1.20 2.74 0.10 1.20 -1.11 0.50 2.22 2.04 3.62 -0.58 -0.82 2.10 1.02 1.44 -1.12
Nucleus 8.15 -19.69 -10.38 -20.53 14.68 8.71 -5.79 -7.26 -9.67 3.16 11.68 -21.55 -16.02 -23.60 14.65
Peroxisomes -4.80 1.61 0.93 5.26 -0.78 -2.86 4.61 3.66 9.01 -1.55 -4.58 3.68 3.22 9.30 -0.91
Vacuole -6.17 -9.71 -27.91 -24.95 -10.60 -1.13 -8.75 -18.65 -18.47 -1.35 -4.34 -11.33 -32.51 -31.17 -9.95
Vacuole Periphery -4.76 3.77 -1.98 0.06 -2.74 -0.04 4.36 4.42 6.78 0.68 -1.43 4.70 -0.13 0.74 -2.72
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
ORF
Human Ortholog
Description Putative protein with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10
Localization
Cell Percentages cytoplasm (87%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YML096W

YML096W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yml096w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available