Standard name
Human Ortholog
Description Zinc finger transcription factor involved in arginine-responsive genes; Zn(2)-Cys(6) binuclear cluster domain type; involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.06 0 0.14 0.65 0.59 0.63 0.68 0.3 0.34 0.38 0.31 0.12 0.28 0.35 0.23 0.14 0.22 0 0 0 0 0.07 0.17
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0.05 0 0.09 0 0 0 0 0 0 0 0 0 0 0.08 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.27 0.3 0.3 0 0.34 0.28 0.18 0.69 0.65 0.61 0.68 0.31 0.11 0.11 0.1 0 0.17 0 0 0.05 0 0.08 0.07
Nucleus 0.78 0.72 0.7 0.58 0.15 0.1 0.11 0.15 0.15 0.14 0.17 0.19 0.7 0.52 0.3 0.38 0.48 0.44 0.68 0.73 0.53 0.52 0.3 0.21
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07
Vac/Vac Membrane 0 0.12 0.11 0.05 0.12 0.07 0.05 0.07 0.05 0 0 0.05 0 0.15 0.28 0.28 0.39 0.28 0.09 0.12 0.24 0.28 0.35 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 1 2 0 0 0 2 2 3 5 2 3 0 0 0 0 0 0 0 1 1 4 7 8
Bud Neck 0 0 0 0 2 1 3 9 1 1 2 2 0 0 0 0 0 0 0 0 0 0 4 3
Bud Site 0 0 1 0 0 0 1 0 3 2 7 7 1 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 11 1 8 60 87 117 156 43 60 36 35 19 46 58 9 8 4 2 7 3 4 15 31
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 1 2 1 0 0 0 1 0 0
Endosome 2 0 2 1 8 3 0 3 1 0 0 0 0 5 6 3 2 1 3 1 0 1 3 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2
Mitochondria 16 48 11 17 3 50 51 41 99 114 59 78 50 18 18 4 1 3 1 3 4 3 15 14
Nucleus 75 127 26 33 14 14 20 35 22 24 16 22 113 84 49 15 27 8 78 141 45 59 63 40
Nuclear Periphery 0 0 0 0 0 1 1 0 1 0 0 1 0 1 0 1 0 0 0 0 0 1 0 0
Nucleolus 4 2 0 0 0 0 0 0 0 1 0 0 2 0 1 1 0 0 9 5 2 1 4 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 0 3 6 2 8 6 5 1 2 3 0 0 1 0 0 0 4 4 3 2 13 13
Vac/Vac Membrane 4 21 4 3 11 11 9 16 7 5 4 6 5 25 46 11 22 5 10 23 20 32 73 61
Unique Cell Count 96 177 37 57 93 147 185 231 144 175 96 114 162 162 164 39 56 18 115 194 85 115 209 190
Labelled Cell Count 110 211 47 65 104 169 212 270 186 214 128 157 190 179 182 45 62 22 115 194 85 115 209 190


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.4 4.7 4.8 5.4 4.2 4.3 4.7 3.5 3.3 3.3 3.4 4.3 4.3 4.6 6.6 7.1 6.9 4.6 4.3 4.5
Std Deviation (1e-4) 1.2 0.8 0.8 1.5 1.7 2.0 1.8 1.8 1.4 1.3 1.4 0.9 0.9 1.1 1.6 1.7 2.3 2.5 1.5 1.4 1.6
Intensity Change (Log2) – – – 0.02 0.2 -0.17 -0.14 -0.01 -0.43 -0.5 -0.5 -0.48 -0.14 -0.12 -0.03 0.49 0.59 0.56 -0.04 -0.12 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 1.9 3.0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9103 2.3796 1.9687 1.258 1.254 1.7792 -0.0724 0.1056 -0.0827 -0.1179 -0.3211 0.0487 0.2646 0.7468 0.361 0.3898 0.4679 0.6277
Actin 0.0094 0.0028 0.0041 0.0003 0.0038 0.0064 0.0216 0.0005 0.0035 0.0174 0.0301 0.004 0.0057 0.0002 0.0014 0.006 0.0009 0.0017
Bud 0.0005 0.0001 0.0001 0 0.0001 0.0002 0.0021 0.0004 0.0006 0.0002 0.0005 0.0004 0.0024 0.0003 0.0007 0.0004 0.0002 0.0013
Bud Neck 0.003 0.0013 0.0007 0.0004 0.002 0.0102 0.0023 0.0004 0.0037 0.0008 0.0019 0.0031 0.0023 0.0008 0.0018 0.0023 0.0014 0.0036
Bud Periphery 0.0005 0.0002 0.0001 0 0.0001 0.0003 0.0019 0.0007 0.0011 0.0004 0.0008 0.0009 0.0028 0.0002 0.0007 0.0007 0.0003 0.0004
Bud Site 0.0043 0.0092 0.0024 0.0002 0.0003 0.0007 0.0059 0.0028 0.003 0.0005 0.0018 0.001 0.0088 0.0014 0.002 0.0013 0.0003 0.0001
Cell Periphery 0.0003 0.0001 0.0001 0 0 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0 0.0002 0.0006 0.0001 0
Cytoplasm 0.0709 0.069 0.0278 0.0166 0.0171 0.0339 0.0194 0.0319 0.0163 0.0099 0.0129 0.0105 0.0177 0.0193 0.0164 0.0074 0.0262 0.0198
Cytoplasmic Foci 0.0171 0.0055 0.0143 0.0004 0.0244 0.0041 0.0198 0.0022 0.0053 0.0015 0.005 0.0033 0.0158 0.0035 0.0067 0.0152 0.0195 0.0059
Eisosomes 0.0004 0.0001 0.0001 0 0 0.0002 0.0002 0 0 0.0003 0.0003 0 0.0003 0.0001 0.0001 0.0007 0.0001 0
Endoplasmic Reticulum 0.0118 0.0026 0.0015 0.0003 0.001 0.0025 0.0033 0.0015 0.001 0.0004 0.0005 0.0004 0.003 0.0009 0.0034 0.0163 0.006 0.0016
Endosome 0.0242 0.0029 0.0133 0.0004 0.0125 0.0104 0.04 0.0031 0.0052 0.003 0.0061 0.0066 0.011 0.0012 0.0076 0.0209 0.0083 0.0096
Golgi 0.0082 0.0002 0.0072 0 0.0299 0.0122 0.0111 0.0006 0.0034 0.0069 0.0077 0.0071 0.0037 0.0001 0.0017 0.0095 0.0006 0.0008
Lipid Particles 0.0138 0.0016 0.0099 0 0.0207 0.0021 0.015 0.0019 0.0044 0.0269 0.0068 0.002 0.0105 0.0004 0.0037 0.0705 0.0226 0.0015
Mitochondria 0.0112 0.0007 0.0031 0.0002 0.0069 0.0045 0.0075 0.0019 0.0175 0.0194 0.0482 0.003 0.0099 0.001 0.0026 0.0243 0.0016 0.0182
None 0.1295 0.114 0.1262 0.0804 0.0668 0.086 0.0226 0.0307 0.0227 0.0021 0.0148 0.0101 0.0762 0.1163 0.0554 0.0116 0.0962 0.0445
Nuclear Periphery 0.0245 0.0155 0.0135 0.0085 0.0104 0.0047 0.0224 0.0305 0.0284 0.009 0.0116 0.0065 0.0194 0.01 0.0107 0.0321 0.0212 0.0096
Nucleolus 0.0111 0.0102 0.0127 0.0071 0.0094 0.0225 0.0079 0.0101 0.0112 0.0099 0.0231 0.0172 0.0299 0.013 0.0149 0.0171 0.0139 0.0174
Nucleus 0.6067 0.7177 0.7151 0.8376 0.7009 0.7484 0.7312 0.8463 0.8251 0.8637 0.7144 0.8827 0.45 0.5168 0.5003 0.3728 0.3702 0.5308
Peroxisomes 0.0042 0.003 0.0076 0 0.0241 0.0009 0.0138 0.0039 0.0091 0.001 0.0281 0.0042 0.0014 0.0001 0.0007 0.009 0.0008 0.0011
Punctate Nuclear 0.0426 0.0412 0.0386 0.0473 0.0688 0.0482 0.0308 0.0289 0.0363 0.0257 0.0837 0.0361 0.3247 0.3135 0.3649 0.3724 0.4065 0.3315
Vacuole 0.0049 0.0019 0.0012 0.0002 0.0005 0.0011 0.0133 0.0012 0.0014 0.0005 0.0009 0.0004 0.0029 0.0007 0.003 0.005 0.0021 0.0003
Vacuole Periphery 0.001 0.0003 0.0004 0.0001 0.0003 0.0003 0.0073 0.0004 0.0006 0.0003 0.0008 0.0002 0.0013 0.0003 0.0012 0.004 0.0011 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9856 14.5549 14.4005 11.5709 14.8853 10.4313 11.9807 14.6443 11.8983 14.2495
Translational Efficiency 0.5503 0.4009 0.3129 0.3556 0.329 0.5901 0.4752 0.2952 0.3622 0.3801

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1532 572 298 165 1887 1208 1325 1576 3419 1780 1623 1741

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 686.74 757.23 970.11 930.39 717.51 791.08 841.18 868.20 703.72 780.20 864.85 874.09
Standard Deviation 70.07 95.43 115.79 104.35 80.44 97.50 102.63 122.89 77.49 98.12 116.41 122.61
Intensity Change Log 2 — 0.140968 0.498384 0.438072 — 0.140825 0.229416 0.275028 — 0.140895 0.367218 0.357067

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000038 0.000670 0.001239 0.001003 0.000052 0.000360 0.000311 0.000811 0.000045 0.000460 0.000481 0.000829
Bud Neck 0.001505 0.005724 0.006627 0.003600 0.002199 0.009567 0.008466 0.018068 0.001888 0.008332 0.008128 0.016696
Bud Site 0.000543 0.002855 0.001379 0.001621 0.000515 0.005205 0.002323 0.009237 0.000528 0.004450 0.002149 0.008515
Cell Periphery 0.000114 0.000131 0.000090 0.000145 0.000056 0.000208 0.000177 0.000139 0.000082 0.000183 0.000161 0.000139
Cytoplasm 0.073550 0.085047 0.012834 0.037784 0.055845 0.104698* 0.028059 0.025765 0.063778 0.098383 0.025263 0.026904
Cytoplasmic Foci 0.010887 0.016589 0.000399 0.000959 0.007945 0.031352 0.001602 0.001035 0.009263 0.026608 0.001381 0.001027
Eisosomes 0.000024 0.000049 0.000046 0.000057 0.000019 0.000051 0.000029 0.000025 0.000021 0.000051 0.000032 0.000028
Endoplasmic Reticulum 0.000677 0.004028 0.011563 0.009387 0.000971 0.003243 0.003855 0.006492 0.000839 0.003495 0.005270 0.006766
Endosome 0.000677 0.001528 0.000445 0.001003 0.000390 0.003832 0.000809 0.001307 0.000518 0.003091 0.000742 0.001278
Golgi 0.000149 0.000461 0.000081 0.000097 0.000272 0.002694 0.000061 0.001765 0.000217 0.001976 0.000064 0.001607
Lipid Particles 0.001788 0.003505 0.000485 0.000947 0.002042 0.005561 0.000581 0.000283 0.001928 0.004900 0.000563 0.000346
Mitochondria 0.001126 0.003757 0.000962 0.001069 0.001331 0.011031 0.001108 0.002839 0.001239 0.008694 0.001081 0.002671
Mitotic Spindle 0.000682 0.001624 0.000380 0.002863 0.002131 0.012574 0.001820 0.008911 0.001482 0.009055 0.001555 0.008338
None 0.011624 0.012614 0.002744 0.011636 0.005467 0.009456 0.009206 0.007037 0.008226 0.010471 0.008019 0.007473
Nuclear Periphery 0.001303 0.001936 0.000875 0.001540 0.001958 0.002783 0.001030 0.001608 0.001664 0.002511 0.001002 0.001602
Nuclear Periphery Foci 0.000569 0.001240 0.000242 0.000746 0.000429 0.001168 0.000203 0.000539 0.000492 0.001191 0.000210 0.000559
Nucleolus 0.009313 0.009910 0.003103 0.002628 0.007106 0.012504 0.003132 0.002924 0.008095 0.011670 0.003127 0.002896
Nucleus 0.882712* 0.840051* 0.949614* 0.905752* 0.909308* 0.766144* 0.931389* 0.902822* 0.897391* 0.789894* 0.934736* 0.903099*
Peroxisomes 0.000472 0.004155 0.000267 0.000259 0.000810 0.004706 0.000737 0.000219 0.000659 0.004529 0.000651 0.000222
Vacuole 0.002123 0.003828 0.006481 0.016761 0.001053 0.011270 0.004910 0.007559 0.001532 0.008879 0.005198 0.008431
Vacuole Periphery 0.000122 0.000299 0.000145 0.000143 0.000102 0.001595 0.000193 0.000616 0.000111 0.001179 0.000184 0.000571

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.96 -13.54 -9.48 -4.51 -1.74 -6.48 -12.03 -14.11 -7.20 -7.83 -7.82 -17.15 -16.34 -6.41 -6.35
Bud Neck -3.53 -8.18 -7.61 -1.09 2.44 -5.91 -6.10 -12.33 -5.86 -6.10 -6.97 -7.94 -13.01 -6.87 -6.01
Bud Site -2.80 -4.63 -4.04 -1.27 -2.56 -4.60 -3.56 -6.81 -2.19 -4.86 -5.32 -4.22 -7.18 -2.86 -5.16
Cell Periphery -1.08 0.79 -2.18 -1.60 -4.22 -4.18 -3.36 -6.74 2.32 1.04 -3.89 -2.36 -3.39 2.28 0.60
Cytoplasm -1.56 13.99 4.40 4.85 -3.76 -8.93 9.16 9.44 15.32 0.49 -7.77 14.82 12.90 16.65 -1.18
Cytoplasmic Foci -2.23 9.86 8.67 6.10 -2.68 -9.81 8.60 9.89 13.49 2.25 -9.31 12.32 13.33 14.78 1.71
Eisosomes -5.36 -7.09 -7.35 -4.00 -4.92 -11.95 -6.15 -7.25 7.23 -0.95 -12.01 -7.78 -9.08 5.00 -2.48
Endoplasmic Reticulum -8.89 -14.48 -10.38 -7.46 -2.02 -7.49 -12.03 -20.88 -10.77 -7.11 -11.34 -17.44 -23.33 -12.29 -5.62
Endosome -2.82 1.35 -3.80 -0.58 -4.69 -7.29 -1.65 -3.00 5.63 -0.85 -7.63 -0.89 -2.90 5.48 -1.58
Golgi -2.19 0.94 -0.46 2.12 -3.28 -3.47 1.37 -1.16 1.96 -1.61 -3.76 1.69 -1.28 1.60 -1.62
Lipid Particles -3.12 5.49 0.35 3.25 -4.34 -4.69 5.64 6.53 7.67 2.22 -5.64 7.47 8.00 9.06 0.62
Mitochondria -1.67 -0.30 -1.20 1.05 -1.18 -6.09 0.65 -3.86 5.12 -4.89 -6.22 0.23 -4.38 4.89 -5.11
Mitotic Spindle -1.68 0.46 -2.44 -2.13 -2.52 -4.31 -1.21 -4.56 0.80 -3.35 -4.63 -1.57 -5.47 -0.48 -4.01
None -0.68 8.42 1.79 2.08 -2.44 -3.18 -2.02 0.20 2.99 1.93 -2.06 1.64 2.93 4.24 1.05
Nuclear Periphery -2.12 0.43 -3.59 -2.10 -4.54 -2.78 2.78 -1.85 1.56 -4.48 -3.63 1.60 -3.54 0.79 -5.72
Nuclear Periphery Foci -2.71 1.40 -2.08 -0.57 -2.52 -3.57 1.45 -0.53 2.81 -1.53 -4.16 1.74 -1.12 2.95 -2.63
Nucleolus -0.60 6.03 6.05 6.80 0.39 -3.75 5.83 6.29 7.58 0.33 -3.33 8.02 8.40 9.48 0.35
Nucleus 4.54 -9.24 2.21 -0.76 6.05 16.04 -3.93 2.01 -14.07 5.45 15.53 -8.01 0.86 -13.55 7.30
Peroxisomes -3.91 2.91 1.29 4.03 -1.45 -6.91 0.53 4.01 8.45 1.39 -7.92 0.35 4.79 9.02 1.38
Vacuole -3.02 -3.96 -5.34 -4.91 -4.40 -8.23 -5.80 -9.83 0.04 -4.04 -8.56 -6.52 -10.43 -3.37 -5.57
Vacuole Periphery -5.16 -2.04 -3.92 1.68 -2.79 -3.39 -2.12 -3.36 2.61 -2.37 -3.58 -2.14 -3.39 2.51 -2.49
Standard name
Human Ortholog
Description Zinc finger transcription factor involved in arginine-responsive genes; Zn(2)-Cys(6) binuclear cluster domain type; involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Arg81

Arg81


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arg81-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available