Standard name
Human Ortholog
Description PtdIns-3-P binding protein that tethers the ER to vacuoles at NVJs; anchored in the ER membrane at nucleus-vacuole junctions and binds phosphatidylinositol 3-phosphate (PtdIns-3-P) in the vacuolar membrane via its Phox homology (PX) domain; expressed predominantly in late G1 to early S phase of the cell cycle; mutation affects nuclear and mitochondrial transmission to daughter buds; similar to 4 human genes, one of which (SNX14) is associated with neurological disease
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Bud Neck 0 0.06 0.1 0.06 0.08 0.06 0.06 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.41 0.6 0.76 0.9 0.88 0.85 0.83 0.87 0.86 0.75 0.65 0.69 0.63 0.59 0.71 0.38 0.41 0.25 0.12 0.21 0.2 0.37 0.31 0.32
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.15 0.09 0 0 0 0 0 0 0 0 0 0 0.17 0.16 0.08 0.19 0.29 0.27 0.36 0.32 0.29 0.2 0.19 0.17
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0 0 0.14 0.23 0.18 0.27 0.34 0.47 0.46 0 0 0 0 0.06 0 0.12 0.12 0.18 0.1 0.17 0.15
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.35 0.27 0.17 0.05 0.06 0 0.05 0 0 0 0 0 0.22 0.22 0.13 0.47 0.35 0.45 0.21 0.19 0.14 0.15 0.18 0.17
Vac/Vac Membrane 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.07 0.06 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 2 0 1 0 0 0 0 0 0 2 0 0 0 1 1 4 5 3 7 5
Bud 0 0 5 3 3 3 10 12 4 5 3 6 1 3 17 0 0 1 1 5 0 3 6 5
Bud Neck 3 15 34 13 19 13 18 17 4 6 3 1 6 15 6 2 3 3 0 6 3 4 3 4
Bud Site 0 0 0 0 0 1 2 3 1 3 0 1 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 0 2 2 1 1 0 0 0 0 0 0 0 0 2 0 1 1
Cytoplasm 28 156 268 188 206 185 249 388 224 218 90 130 131 172 199 26 21 19 13 75 68 78 87 108
Endoplasmic Reticulum 0 2 0 0 3 1 0 0 1 0 0 0 5 11 2 2 2 3 0 3 4 2 4 10
Endosome 10 23 0 0 0 0 0 0 1 0 0 0 35 46 22 13 15 20 38 116 101 42 54 59
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 1 2
Mitochondria 2 11 17 5 5 31 68 80 71 99 66 86 3 2 11 0 3 2 13 42 62 22 48 52
Nucleus 0 0 1 1 0 0 1 0 3 1 0 1 2 2 0 1 1 0 0 0 1 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 1 1 1 2 0 0 0 0 0 0 1 2 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 2 1 0 1 0 0 0 3 1 0 2 1 3 1 1 0 0
Peroxisomes 2 0 0 0 0 0 0 0 0 0 0 0 3 10 6 1 0 2 0 1 2 1 1 0
SpindlePole 24 69 61 10 13 9 15 16 11 5 1 5 46 64 37 32 18 34 22 68 48 32 49 58
Vac/Vac Membrane 6 5 0 0 0 0 0 0 0 0 0 2 0 1 1 2 0 1 8 20 24 13 12 26
Unique Cell Count 68 258 354 209 235 217 300 448 261 291 139 189 209 290 279 68 51 75 107 362 349 212 284 343
Labelled Cell Count 76 283 387 220 251 245 364 518 324 340 166 235 232 329 306 80 64 88 107 362 349 212 284 343


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 3.7 2.9 2.7 3.0 2.6 2.6 2.7 2.4 2.3 2.4 2.3 4.0 4.3 3.7 6.2 6.3 6.1 4.2 4.2 4.6
Std Deviation (1e-4) 1.1 0.9 0.6 0.7 0.9 0.6 0.8 0.8 1.2 1.0 1.2 1.0 1.0 1.0 0.8 2.2 2.2 2.0 1.3 1.2 1.2
Intensity Change (Log2) – – – -0.08 0.06 -0.15 -0.17 -0.1 -0.24 -0.34 -0.24 -0.34 0.49 0.57 0.34 1.1 1.12 1.09 0.54 0.54 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7004 -0.1285 -0.2059 -0.1848 -0.1824 -0.135 2.2921 2.6478 2.5945 3.0065 2.6184 3.0142 2.5393 2.3822 2.4857 2.7661 3.152 3.0939
Actin 0.035 0.0002 0.0055 0.0006 0.0696 0.0023 0.0195 0.0003 0.0002 0.0197 0.002 0.0062 0.0109 0 0.0002 0.0002 0.0002 0.0008
Bud 0.0002 0 0.0001 0.0001 0.0019 0.0002 0.0007 0.002 0.0019 0.0005 0.0012 0.0001 0.0001 0.0001 0 0 0.0003 0.0002
Bud Neck 0.0091 0.0079 0.0059 0.0011 0.0161 0.0066 0.0075 0.0021 0.0049 0.0005 0.0344 0.0017 0.0007 0.0046 0.0003 0.0055 0.0004 0.0028
Bud Periphery 0.0003 0.0001 0.0003 0.0001 0.0098 0.0002 0.0008 0.0015 0.001 0.0009 0.002 0.0002 0.0001 0.0001 0.0001 0 0.0005 0.0007
Bud Site 0.001 0.0014 0.0007 0.0002 0.0017 0.0004 0.0095 0.006 0.0048 0.0022 0.0106 0.0001 0.0012 0.0006 0.0004 0.0001 0.0001 0.0002
Cell Periphery 0.0003 0.0003 0.0002 0.0002 0.0004 0.0004 0.0007 0.0002 0.0003 0.0002 0.0005 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0799 0.082 0.0835 0.1319 0.0469 0.1562 0.0387 0.0458 0.035 0.0225 0.0436 0.0728 0.0513 0.0755 0.06 0.0201 0.0458 0.072
Cytoplasmic Foci 0.1549 0.1667 0.187 0.1893 0.1837 0.1688 0.0945 0.09 0.103 0.0906 0.1408 0.0941 0.1442 0.141 0.209 0.2705 0.1634 0.1711
Eisosomes 0.0004 0.0002 0.0003 0.0001 0.0004 0.0001 0.0007 0.0002 0.0002 0.0006 0.0003 0.0001 0.0004 0.0001 0.0002 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0046 0.0047 0.0035 0.0059 0.0026 0.005 0.0032 0.0016 0.002 0.002 0.0018 0.0034 0.0022 0.0032 0.0018 0.0005 0.0016 0.0028
Endosome 0.061 0.0602 0.0704 0.068 0.078 0.0452 0.0227 0.0227 0.0211 0.0286 0.0212 0.0183 0.0312 0.0583 0.0496 0.0429 0.0155 0.0291
Golgi 0.005 0.0022 0.0025 0.0015 0.0035 0.0016 0.0029 0.0011 0.0007 0.0026 0.0015 0.0011 0.0018 0.0019 0.0017 0.0018 0.0006 0.0016
Lipid Particles 0.1125 0.0764 0.0869 0.0677 0.0914 0.0603 0.0245 0.0333 0.0237 0.0498 0.0324 0.0196 0.0347 0.0229 0.0478 0.0648 0.0257 0.0404
Mitochondria 0.0014 0.0004 0.0023 0.0005 0.002 0.0007 0.0021 0.0006 0.0006 0.0083 0.0023 0.0007 0.0005 0.0003 0.0003 0.0001 0.0004 0.0005
None 0.4962 0.5614 0.5157 0.5023 0.4319 0.4984 0.7381 0.7555 0.7666 0.7164 0.6646 0.7403 0.6966 0.6345 0.5879 0.5783 0.7313 0.6493
Nuclear Periphery 0.0053 0.004 0.0027 0.0054 0.003 0.0025 0.0048 0.0019 0.0017 0.0053 0.0021 0.0042 0.0013 0.0021 0.0015 0.0002 0.0008 0.0014
Nucleolus 0.0013 0.0028 0.0018 0.0007 0.0036 0.0032 0.0035 0.0028 0.0027 0.0021 0.0029 0.0017 0.0009 0.0019 0.0011 0.0013 0.0006 0.0011
Nucleus 0.0025 0.0025 0.0016 0.0029 0.0023 0.0066 0.0046 0.0034 0.0024 0.0027 0.0025 0.0037 0.0016 0.003 0.0015 0.0004 0.0007 0.0012
Peroxisomes 0.0068 0.0025 0.0033 0.0022 0.0043 0.0033 0.0048 0.0016 0.002 0.0072 0.0046 0.0035 0.0056 0.0013 0.0041 0.0037 0.0018 0.0068
Punctate Nuclear 0.0149 0.0188 0.021 0.0077 0.0418 0.0297 0.0126 0.0238 0.0211 0.0278 0.0254 0.0253 0.0113 0.0409 0.0295 0.0081 0.0076 0.015
Vacuole 0.0067 0.0051 0.0044 0.0107 0.004 0.0077 0.0028 0.0034 0.004 0.0085 0.003 0.0023 0.0027 0.007 0.0026 0.0012 0.0023 0.0024
Vacuole Periphery 0.0007 0.0004 0.0005 0.0009 0.0011 0.0005 0.0008 0.0002 0.0003 0.001 0.0005 0.0003 0.0002 0.0006 0.0002 0.0001 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.6729 13.1167 12.3287 13.6071 15.2193 13.0354 19.4514 19.4936 15.8343 20.4903
Translational Efficiency 0.2237 0.2115 0.2026 0.176 0.1511 0.2044 0.1935 0.1403 0.159 0.1473

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
530 1528 1858 1033 1600 1926 583 640 2130 3454 2441 1673

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 590.86 625.77 821.01 782.08 618.98 670.50 758.81 892.14 611.98 650.71 806.15 824.18
Standard Deviation 65.57 76.99 95.93 126.32 71.93 89.84 88.60 112.69 71.44 87.27 97.89 132.56
Intensity Change Log 2 — 0.082816 0.474583 0.404500 — 0.115345 0.293846 0.527377 — 0.099550 0.384944 0.468673

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000065 0.000145 0.000293 0.003129 0.000081 0.000296 0.000323 0.000299 0.000077 0.000229 0.000300 0.002046
Bud Neck 0.026586 0.024333 0.022277 0.011767 0.015786 0.027325 0.003581 0.025533 0.018474 0.026001 0.017811 0.017033
Bud Site 0.002020 0.005064 0.028668 0.042851 0.001884 0.010275 0.004177 0.036214 0.001918 0.007970 0.022818 0.040312
Cell Periphery 0.000186 0.000095 0.000268 0.000196 0.000100 0.000150 0.000240 0.000160 0.000121 0.000126 0.000261 0.000182
Cytoplasm 0.264420* 0.324260* 0.392182* 0.422157* 0.300463* 0.174218* 0.346242* 0.641875* 0.291494* 0.240594* 0.381210* 0.506210*
Cytoplasmic Foci 0.378048* 0.291663* 0.044046 0.067139 0.345254* 0.346658* 0.077586 0.010033 0.353414* 0.322329* 0.052056 0.045293
Eisosomes 0.000330 0.000116 0.000047 0.000075 0.000152 0.000209 0.000114 0.000021 0.000197 0.000168 0.000063 0.000055
Endoplasmic Reticulum 0.002571 0.001442 0.003450 0.002194 0.001586 0.001850 0.001395 0.000556 0.001831 0.001669 0.002959 0.001567
Endosome 0.057778 0.048208 0.014628 0.018981 0.042670 0.099496 0.012111 0.002406 0.046429 0.076807 0.014027 0.012641
Golgi 0.004684 0.004008 0.000212 0.007714 0.002999 0.019161 0.001955 0.000156 0.003418 0.012458 0.000628 0.004823
Lipid Particles 0.082513 0.034767 0.003648 0.004216 0.083569 0.091253 0.006084 0.000613 0.083306 0.066265 0.004230 0.002838
Mitochondria 0.008010 0.013777 0.001291 0.012124 0.007798 0.033457 0.001297 0.006208 0.007850 0.024751 0.001292 0.009861
Mitotic Spindle 0.000342 0.023585 0.017136 0.087780 0.006322 0.020876 0.004495 0.038940 0.004834 0.022075 0.014117 0.069097
None 0.002972 0.006088 0.007889 0.003229 0.005791 0.006015 0.012221 0.005231 0.005090 0.006048 0.008923 0.003995
Nuclear Periphery 0.000945 0.002907 0.006009 0.003278 0.002588 0.002125 0.002168 0.001336 0.002180 0.002471 0.005091 0.002535
Nuclear Periphery Foci 0.001672 0.004043 0.019922 0.005395 0.007279 0.002522 0.003693 0.000145 0.005884 0.003195 0.016046 0.003386
Nucleolus 0.006086 0.010519 0.017000 0.001644 0.010038 0.008192 0.010192 0.001529 0.009055 0.009222 0.015374 0.001600
Nucleus 0.096574 0.127360 0.380421* 0.147202* 0.123126 0.068088 0.464575* 0.182708* 0.116519 0.094309 0.400520* 0.160785*
Peroxisomes 0.016562 0.015458 0.001559 0.003423 0.011547 0.024458 0.002507 0.000993 0.012795 0.020476 0.001786 0.002493
Vacuole 0.045990 0.059552 0.038627 0.149464 0.030013 0.058820 0.044714 0.044343 0.033989 0.059144 0.040081 0.109250
Vacuole Periphery 0.001646 0.002611 0.000429 0.006042 0.000952 0.004556 0.000332 0.000702 0.001125 0.003695 0.000406 0.003999

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.75 -7.01 -2.12 -1.99 -1.69 -5.94 -1.61 -1.67 0.72 0.48 -7.29 -5.79 -2.24 -1.86 -1.59
Bud Neck 0.76 -1.05 4.50 6.12 9.49 -6.06 7.87 -4.42 -0.28 -8.60 -4.51 -3.32 0.29 4.75 3.56
Bud Site -4.88 -9.67 -7.79 -6.79 -0.11 -7.35 -1.85 -6.37 -4.04 -5.43 -8.63 -9.88 -10.10 -7.32 -1.95
Cell Periphery 3.43 -1.24 1.18 -5.26 3.37 -3.78 -6.34 -1.12 1.19 3.75 -0.27 -5.97 -1.63 -1.46 4.63
Cytoplasm -3.96 -5.28 -7.60 -4.62 -3.62 13.66 -1.09 -21.13 -31.17 -16.82 6.38 -5.04 -17.41 -23.78 -13.23
Cytoplasmic Foci 6.61 31.53 29.67 33.41 -1.58 1.41 29.26 51.91 56.93 11.58 5.50 51.35 54.40 58.90 5.18
Eisosomes 9.03 11.27 10.45 6.80 -4.34 -3.75 0.42 25.98 12.76 14.70 2.80 15.07 18.52 12.67 6.46
Endoplasmic Reticulum 1.43 -2.80 -0.62 -6.05 5.56 -1.40 -2.61 3.19 5.30 6.04 0.45 -8.33 -1.58 -3.02 8.69
Endosome 1.45 8.28 9.79 13.99 3.74 -14.44 8.87 17.70 28.92 6.59 -10.63 12.65 17.95 29.98 7.13
Golgi 0.54 3.10 0.99 0.90 -3.19 -12.18 1.59 13.83 14.44 1.55 -10.58 6.35 2.17 11.23 -2.35
Lipid Particles 7.75 13.30 13.37 14.77 1.06 -1.72 19.69 21.92 24.87 9.63 4.19 24.40 25.22 27.79 6.90
Mitochondria -2.64 4.85 1.34 3.90 -2.62 -12.34 7.72 2.07 12.68 -3.17 -12.02 9.84 2.10 12.00 -3.73
Mitotic Spindle -9.43 -10.36 -10.28 -4.86 -4.91 -5.72 0.71 -5.21 -2.54 -5.26 -9.41 -7.44 -10.64 -5.74 -6.09
None -4.78 -6.96 -0.59 5.11 7.67 -0.11 -2.41 1.64 1.11 2.96 -1.28 -4.36 2.86 3.60 7.00
Nuclear Periphery -3.80 -16.69 -11.17 -2.99 7.24 1.69 -0.60 -1.47 -3.04 -0.93 -0.58 -12.26 -6.01 -4.88 7.37
Nuclear Periphery Foci -5.37 -13.04 -5.75 0.25 11.20 7.00 -1.07 9.32 7.47 5.06 4.28 -10.29 4.63 0.72 12.54
Nucleolus -4.65 -10.72 2.58 8.05 13.09 3.48 -3.96 7.11 3.81 6.29 0.24 -9.89 9.02 9.05 14.38
Nucleus -3.19 -29.49 -10.13 -8.38 19.30 9.98 -23.56 -11.31 -17.22 13.82 4.97 -38.88 -14.37 -18.80 23.09
Peroxisomes -0.04 7.65 7.38 13.96 -0.57 -9.89 10.51 17.65 21.03 4.61 -7.86 16.06 16.46 24.40 2.05
Vacuole -2.69 -1.47 -21.96 -21.67 -22.65 -10.39 -6.73 -9.07 -3.87 -2.44 -10.82 -8.46 -24.77 -20.43 -20.65
Vacuole Periphery -2.03 4.45 -1.24 -0.14 -3.37 -11.44 5.65 2.95 12.46 -1.83 -11.27 7.08 -1.66 4.30 -3.52
Standard name
Human Ortholog
Description PtdIns-3-P binding protein that tethers the ER to vacuoles at NVJs; anchored in the ER membrane at nucleus-vacuole junctions and binds phosphatidylinositol 3-phosphate (PtdIns-3-P) in the vacuolar membrane via its Phox homology (PX) domain; expressed predominantly in late G1 to early S phase of the cell cycle; mutation affects nuclear and mitochondrial transmission to daughter buds; similar to 4 human genes, one of which (SNX14) is associated with neurological disease
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Mdm1

Mdm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available