ORF | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 1 | 0 | 2 | 5 | 7 | 12 | 14 | 15 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 7 | 13 | 11 | 10 | 10 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 5 | 18 | 12 | 14 | 13 | 14 | 9 | 18 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 18 | 13 | 155 | 152 | 38 | 39 | 32 | 104 | 37 | 36 | 43 | 67 | 26 | 47 | 41 | 9 | 15 | 17 | 13 | 10 | 109 | 30 | 6 | 6 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 3 | 0 | 0 | 9 | 2 | 0 | 3 |
Endosome | 2 | 5 | 21 | 24 | 8 | 2 | 8 | 12 | 4 | 5 | 0 | 17 | 0 | 4 | 1 | 1 | 2 | 0 | 5 | 2 | 25 | 2 | 2 | 5 |
Golgi | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
Mitochondria | 8 | 0 | 9 | 16 | 11 | 198 | 211 | 94 | 201 | 290 | 394 | 273 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 3 | 1 | 3 | 3 |
Nucleus | 204 | 66 | 255 | 238 | 596 | 713 | 796 | 675 | 610 | 688 | 582 | 695 | 32 | 146 | 169 | 73 | 84 | 71 | 252 | 142 | 439 | 85 | 129 | 112 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 10 | 7 | 33 | 28 | 15 | 17 | 6 | 18 | 0 | 0 | 6 | 1 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 5 |
Nucleolus | 0 | 1 | 0 | 2 | 17 | 42 | 95 | 47 | 25 | 46 | 44 | 38 | 0 | 2 | 1 | 2 | 3 | 1 | 0 | 2 | 5 | 3 | 5 | 9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 2 | 2 | 11 | 2 | 1 | 4 |
Vac/Vac Membrane | 79 | 95 | 308 | 180 | 35 | 27 | 35 | 99 | 39 | 54 | 50 | 101 | 42 | 47 | 16 | 23 | 48 | 43 | 19 | 11 | 71 | 45 | 55 | 72 |
Unique Cell Count | 273 | 162 | 635 | 540 | 686 | 873 | 1018 | 938 | 810 | 933 | 872 | 1011 | 88 | 237 | 227 | 106 | 139 | 125 | 308 | 181 | 712 | 192 | 220 | 240 |
Labelled Cell Count | 311 | 180 | 754 | 616 | 724 | 1048 | 1232 | 1080 | 958 | 1166 | 1145 | 1243 | 101 | 251 | 238 | 114 | 153 | 135 | 308 | 181 | 712 | 192 | 220 | 240 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 7.8 | 7.8 | 7.2 | 6.8 | 5.3 | 5.6 | 6.6 | 5.6 | 5.4 | 5.1 | 5.4 | 8.5 | 8.6 | 8.4 | 11.2 | 11.7 | 11.7 | 7.9 | 8.1 | 8.3 |
Std Deviation (1e-4) | 1.2 | 1.5 | 1.3 | 1.4 | 1.9 | 1.7 | 1.6 | 2.0 | 1.4 | 1.2 | 1.2 | 1.3 | 1.4 | 1.8 | 1.7 | 2.9 | 2.8 | 3.0 | 1.6 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.2 | -0.57 | -0.49 | -0.25 | -0.48 | -0.54 | -0.62 | -0.54 | 0.12 | 0.13 | 0.1 | 0.52 | 0.58 | 0.58 | 0.01 | 0.06 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 3.1 | 3.1 | 3.3 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 3.6 | 0 | 3.1 | 3.2 | 3.1 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.5 | -9.7 | -11.4 | -13.3 | -7.0 | -11.0 | -12.2 | -11.1 | -10.3 | 1.0 | -1.4 | -2.0 | -3.7 | -3.5 | -2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.0 | -2.7 | -4.8 | -3.8 | -2.8 | -4.1 | -4.2 | -5.4 | -2.1 | 0 | -1.3 | -2.4 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.8 | 0.3 | 11.8 | 11.2 | 6.8 | 12.5 | 14.7 | 19.0 | 13.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.4 | 17.7 | 16.6 | 15.6 | 12.6 | 13.5 | 13.3 | 10.3 | 11.4 | -0.7 | 5.7 | 8.9 | 5.5 | 4.4 | 3.4 |
Nuclear Periphery | 0 | 2.6 | 0 | 4.3 | 4.1 | 3.1 | 3.0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 4.0 | 5.6 | 7.9 | 5.7 | 4.5 | 5.7 | 5.7 | 4.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -5.3 | -18.0 | -20.9 | -22.0 | -16.9 | -19.3 | -19.7 | -19.3 | -17.6 | -0.1 | -7.7 | -11.1 | -5.1 | -3.0 | -2.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.7244 | 4.8162 | 4.2021 | 3.9813 | 3.4934 | 3.7891 | 5.1497 | 6.7015 | 5.9372 | 5.5802 | 5.7003 | 5.8708 | 4.0764 | 5.5081 | 5.1543 | 4.9497 | 4.3936 | 4.8869 |
Actin | 0.0346 | 0 | 0.0001 | 0.0031 | 0 | 0.0001 | 0.0161 | 0.0043 | 0.0176 | 0.0036 | 0.0666 | 0.0023 | 0.0067 | 0.002 | 0.0127 | 0.0203 | 0 | 0.0011 |
Bud | 0.0004 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0007 | 0.0001 | 0.001 | 0.0005 | 0.0001 | 0.0007 | 0.0004 | 0.0007 | 0 | 0.0004 |
Bud Neck | 0.0027 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0025 | 0.0055 | 0.0004 | 0.001 | 0.0004 | 0.0024 | 0.0015 | 0.0002 | 0.0004 | 0.0002 | 0.0008 | 0.0005 | 0.0016 |
Bud Periphery | 0.001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0008 | 0.0001 | 0.0013 | 0.0003 | 0.0023 | 0.0005 | 0 | 0.0005 | 0.0002 | 0.0005 | 0 | 0.0003 |
Bud Site | 0.0011 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0004 | 0.0043 | 0.0012 | 0.0023 | 0.001 | 0.0024 | 0.0004 | 0.0001 | 0.0123 | 0.0016 | 0.0009 | 0 | 0.0009 |
Cell Periphery | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0011 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.021 | 0.0315 | 0.0331 | 0.0271 | 0.0155 | 0.0315 | 0.0135 | 0.0787 | 0.0357 | 0.0109 | 0.0181 | 0.0182 | 0.0189 | 0.0568 | 0.0042 | 0.1358 | 0.0464 | 0.0059 |
Cytoplasmic Foci | 0.0056 | 0.0001 | 0.0024 | 0.01 | 0 | 0.0011 | 0.0122 | 0.0025 | 0.0035 | 0.0008 | 0.0026 | 0.0022 | 0.0194 | 0.0013 | 0.0145 | 0.0106 | 0.0001 | 0.0117 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0028 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0062 | 0.0003 | 0.001 | 0.0004 | 0.0002 | 0.0007 | 0.0055 | 0.0007 | 0.0017 | 0.0061 | 0.0027 | 0.0019 | 0.0063 | 0.0013 | 0.0004 | 0.0056 | 0.0005 | 0.0002 |
Endosome | 0.015 | 0.0003 | 0.0021 | 0.0015 | 0.0001 | 0.0018 | 0.0244 | 0.0011 | 0.0052 | 0.0087 | 0.0079 | 0.0074 | 0.0066 | 0.0028 | 0.0075 | 0.072 | 0.0003 | 0.0114 |
Golgi | 0.003 | 0 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0049 | 0.0011 | 0.0027 | 0.0015 | 0.0055 | 0.002 | 0.0029 | 0.0007 | 0.0087 | 0.0728 | 0 | 0.0069 |
Lipid Particles | 0.0049 | 0 | 0.0023 | 0.0005 | 0 | 0 | 0.0099 | 0.0022 | 0.0027 | 0.0019 | 0.0049 | 0.0054 | 0.0228 | 0.0006 | 0.0004 | 0.0008 | 0 | 0.002 |
Mitochondria | 0.0027 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0093 | 0.001 | 0.0099 | 0.042 | 0.0262 | 0.0032 | 0.0005 | 0.0074 | 0.002 | 0.0068 | 0.0002 | 0.0016 |
None | 0.0028 | 0.0002 | 0.0011 | 0.001 | 0.0001 | 0.0004 | 0.0133 | 0.0007 | 0.002 | 0.001 | 0.0016 | 0.0018 | 0.007 | 0.0006 | 0.0002 | 0.0006 | 0.004 | 0.0002 |
Nuclear Periphery | 0.0322 | 0.0084 | 0.016 | 0.0051 | 0.0066 | 0.0091 | 0.0172 | 0.0098 | 0.0103 | 0.0147 | 0.0092 | 0.0074 | 0.0051 | 0.0069 | 0.0014 | 0.0091 | 0.0081 | 0.0021 |
Nucleolus | 0.0071 | 0.0024 | 0.0031 | 0.0015 | 0.0075 | 0.0058 | 0.0076 | 0.0077 | 0.0042 | 0.0015 | 0.031 | 0.004 | 0.0033 | 0.003 | 0.0017 | 0.0004 | 0.0023 | 0.0073 |
Nucleus | 0.841 | 0.9535 | 0.9309 | 0.9096 | 0.9678 | 0.9316 | 0.8149 | 0.8802 | 0.8834 | 0.9008 | 0.7916 | 0.9269 | 0.8951 | 0.8987 | 0.9395 | 0.6574 | 0.9348 | 0.9362 |
Peroxisomes | 0.0016 | 0 | 0.0001 | 0.0013 | 0 | 0.0001 | 0.0046 | 0.0037 | 0.0045 | 0.0005 | 0.001 | 0.0009 | 0.0025 | 0.0006 | 0.0032 | 0.0004 | 0 | 0.0062 |
Punctate Nuclear | 0.0122 | 0.0022 | 0.006 | 0.0371 | 0.0013 | 0.0128 | 0.0276 | 0.0032 | 0.0084 | 0.0016 | 0.0033 | 0.0079 | 0.0018 | 0.0021 | 0.001 | 0.0014 | 0.0021 | 0.0035 |
Vacuole | 0.0028 | 0.0005 | 0.0006 | 0.0003 | 0.0002 | 0.0012 | 0.0051 | 0.001 | 0.0021 | 0.0015 | 0.0125 | 0.0045 | 0.0004 | 0.0009 | 0.0001 | 0.0018 | 0.0004 | 0.0005 |
Vacuole Periphery | 0.0015 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0017 | 0.0002 | 0.0006 | 0.0008 | 0.003 | 0.001 | 0.0002 | 0.0003 | 0.0001 | 0.0012 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.4935 | 31.0181 | 34.5252 | 28.3677 | 49.4866 | 31.2111 | 25.0899 | 17.9924 | 28.7281 | 33.0207 |
Translational Efficiency | 2.4071 | 2.266 | 1.5401 | 2.3043 | 1.1042 | 2.0613 | 2.4541 | 2.1803 | 1.3315 | 1.7543 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1279 | 2092 | 2534 | 1415 | 1625 | 2044 | 2698 | 2381 | 2904 | 4136 | 5232 | 3796 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 782.01 | 987.89 | 1124.11 | 1298.26 | 832.51 | 929.50 | 1170.24 | 1211.47 | 810.27 | 959.03 | 1147.90 | 1243.82 |
Standard Deviation | 107.70 | 150.49 | 176.59 | 240.52 | 126.51 | 142.76 | 171.78 | 193.27 | 121.22 | 149.60 | 175.64 | 216.23 |
Intensity Change Log 2 | — | 0.337163 | 0.523524 | 0.731320 | — | 0.158987 | 0.491265 | 0.541219 | — | 0.248039 | 0.506980 | 0.636427 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000185 | 0.000288 | 0.000347 | 0.001329 | 0.000205 | 0.000245 | 0.000289 | 0.000550 | 0.000196 | 0.000267 | 0.000317 | 0.000841 |
Bud Neck | 0.004635 | 0.006330 | 0.014613 | 0.029383 | 0.005082 | 0.010480 | 0.014437 | 0.022585 | 0.004885 | 0.008381 | 0.014522 | 0.025119 |
Bud Site | 0.001490 | 0.001762 | 0.001421 | 0.005796 | 0.001421 | 0.003218 | 0.000877 | 0.003132 | 0.001451 | 0.002481 | 0.001140 | 0.004125 |
Cell Periphery | 0.000205 | 0.000137 | 0.000117 | 0.000395 | 0.000215 | 0.000231 | 0.000093 | 0.000125 | 0.000210 | 0.000184 | 0.000104 | 0.000226 |
Cytoplasm | 0.042097 | 0.066518 | 0.017906 | 0.011409 | 0.092865 | 0.137703* | 0.005307 | 0.009055 | 0.070506 | 0.101697* | 0.011409 | 0.009932 |
Cytoplasmic Foci | 0.003799 | 0.001147 | 0.000032 | 0.001795 | 0.002709 | 0.004410 | 0.000026 | 0.000148 | 0.003189 | 0.002760 | 0.000029 | 0.000762 |
Eisosomes | 0.000016 | 0.000011 | 0.000014 | 0.000030 | 0.000014 | 0.000017 | 0.000019 | 0.000021 | 0.000015 | 0.000014 | 0.000017 | 0.000025 |
Endoplasmic Reticulum | 0.003993 | 0.003848 | 0.002376 | 0.002716 | 0.003724 | 0.003298 | 0.002322 | 0.003107 | 0.003843 | 0.003576 | 0.002348 | 0.002961 |
Endosome | 0.000872 | 0.000559 | 0.000173 | 0.003790 | 0.000619 | 0.001758 | 0.000187 | 0.000573 | 0.000730 | 0.001151 | 0.000180 | 0.001772 |
Golgi | 0.000486 | 0.000214 | 0.000041 | 0.002401 | 0.000082 | 0.000773 | 0.000030 | 0.000189 | 0.000260 | 0.000490 | 0.000035 | 0.001013 |
Lipid Particles | 0.000692 | 0.000181 | 0.000051 | 0.000313 | 0.000331 | 0.000640 | 0.000052 | 0.000059 | 0.000490 | 0.000408 | 0.000051 | 0.000154 |
Mitochondria | 0.001204 | 0.000759 | 0.001361 | 0.004713 | 0.000590 | 0.003352 | 0.000961 | 0.001976 | 0.000860 | 0.002041 | 0.001155 | 0.002996 |
Mitotic Spindle | 0.001953 | 0.002038 | 0.002201 | 0.008412 | 0.002086 | 0.009883 | 0.000893 | 0.007858 | 0.002028 | 0.005915 | 0.001526 | 0.008064 |
None | 0.006703 | 0.004391 | 0.004224 | 0.004939 | 0.009150 | 0.010170 | 0.002039 | 0.004234 | 0.008072 | 0.007247 | 0.003097 | 0.004497 |
Nuclear Periphery | 0.001100 | 0.001349 | 0.001143 | 0.001691 | 0.001066 | 0.001660 | 0.000699 | 0.002056 | 0.001081 | 0.001503 | 0.000914 | 0.001920 |
Nuclear Periphery Foci | 0.000387 | 0.000320 | 0.000058 | 0.000835 | 0.000580 | 0.000678 | 0.000096 | 0.000883 | 0.000495 | 0.000497 | 0.000078 | 0.000865 |
Nucleolus | 0.003306 | 0.001224 | 0.000740 | 0.005570 | 0.002273 | 0.003444 | 0.000624 | 0.002146 | 0.002728 | 0.002321 | 0.000681 | 0.003422 |
Nucleus | 0.923459* | 0.904526* | 0.951369* | 0.906226* | 0.872954* | 0.795482* | 0.969955* | 0.937359* | 0.895197* | 0.850637* | 0.960954* | 0.925754* |
Peroxisomes | 0.000371 | 0.000245 | 0.000026 | 0.000322 | 0.000166 | 0.000578 | 0.000022 | 0.000079 | 0.000256 | 0.000410 | 0.000024 | 0.000170 |
Vacuole | 0.002796 | 0.003946 | 0.001716 | 0.005745 | 0.003669 | 0.011175 | 0.001000 | 0.002475 | 0.003284 | 0.007519 | 0.001347 | 0.003694 |
Vacuole Periphery | 0.000252 | 0.000209 | 0.000072 | 0.002189 | 0.000200 | 0.000805 | 0.000072 | 0.001390 | 0.000223 | 0.000503 | 0.000072 | 0.001688 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.21 | -9.93 | -7.18 | -6.08 | -5.51 | -1.69 | -7.44 | -5.74 | -4.59 | -3.39 | -2.71 | -12.40 | -9.17 | -7.37 | -6.30 |
Bud Neck | -2.00 | -14.14 | -15.20 | -14.06 | -8.14 | -5.25 | -14.47 | -14.94 | -9.67 | -4.99 | -5.17 | -20.27 | -21.23 | -16.97 | -9.07 |
Bud Site | -0.50 | -0.58 | -4.82 | -4.87 | -5.27 | -4.40 | 1.68 | -3.45 | 0.35 | -4.37 | -3.20 | 0.15 | -5.69 | -3.11 | -6.53 |
Cell Periphery | 2.25 | 3.14 | -2.03 | -3.79 | -4.36 | -0.25 | 5.92 | 4.56 | 3.38 | -2.92 | 1.32 | 6.25 | 0.84 | -0.44 | -4.86 |
Cytoplasm | -5.47 | 7.74 | 9.63 | 16.54 | 4.75 | -6.87 | 19.56 | 18.25 | 25.42 | -4.42 | -7.66 | 20.33 | 20.37 | 29.87 | 1.28 |
Cytoplasmic Foci | 2.83 | 4.14 | 2.73 | -0.31 | -5.47 | -2.56 | 5.84 | 5.62 | 7.84 | -2.93 | 0.59 | 6.66 | 5.50 | 7.32 | -5.96 |
Eisosomes | 4.62 | -0.60 | -8.59 | -12.58 | -9.22 | -2.93 | -5.87 | -5.64 | -2.50 | -1.04 | 0.78 | -4.70 | -9.74 | -10.80 | -7.33 |
Endoplasmic Reticulum | -0.09 | 3.53 | 3.78 | 4.03 | 0.91 | 1.36 | 5.72 | 2.72 | 1.64 | -1.91 | 0.66 | 6.38 | 4.38 | 3.89 | -0.86 |
Endosome | 1.91 | 4.81 | -2.12 | -2.62 | -3.17 | -3.79 | 5.89 | 1.93 | 4.35 | -2.63 | -2.50 | 7.40 | -1.50 | 0.12 | -3.70 |
Golgi | 0.99 | 1.78 | -1.96 | -2.68 | -3.14 | -2.37 | 2.82 | -1.27 | 2.12 | -2.10 | -1.25 | 1.99 | -1.93 | -0.57 | -3.47 |
Lipid Particles | 2.14 | 2.59 | 1.82 | -0.49 | -1.65 | -2.39 | 6.31 | 6.55 | 4.61 | -0.70 | 0.64 | 3.96 | 3.23 | 3.98 | -1.75 |
Mitochondria | 1.49 | -1.60 | -6.47 | -8.76 | -6.67 | -4.44 | -8.94 | -9.51 | 1.78 | -5.89 | -3.47 | -4.34 | -9.57 | -2.96 | -8.44 |
Mitotic Spindle | -0.09 | -2.39 | -3.69 | -3.85 | -1.97 | -4.60 | -0.29 | -3.68 | 1.40 | -3.69 | -4.02 | -1.95 | -5.13 | -1.16 | -3.88 |
None | 2.76 | 3.13 | 2.11 | -0.69 | -1.09 | -0.55 | 7.11 | 4.68 | 5.18 | -3.49 | 1.22 | 7.50 | 5.08 | 4.21 | -2.95 |
Nuclear Periphery | -1.15 | -2.61 | -2.48 | -1.48 | -0.96 | -3.43 | 0.90 | -2.26 | -0.43 | -2.53 | -3.10 | -1.93 | -3.04 | -1.14 | -2.43 |
Nuclear Periphery Foci | 0.59 | 4.43 | -1.13 | -1.53 | -3.44 | -0.82 | 5.87 | -0.31 | 0.38 | -2.77 | -0.43 | 7.41 | -0.83 | -0.43 | -3.88 |
Nucleolus | 4.26 | 5.08 | -4.78 | -7.31 | -7.69 | -2.29 | 5.03 | -0.93 | 1.50 | -5.28 | 1.32 | 7.05 | -4.36 | -5.36 | -9.23 |
Nucleus | 3.24 | -3.99 | 2.66 | -0.32 | 7.38 | 9.88 | -16.40 | -9.98 | -20.30 | 8.45 | 8.92 | -15.25 | -6.27 | -16.23 | 10.40 |
Peroxisomes | 0.90 | 3.18 | 0.72 | -0.43 | -4.90 | -2.62 | 3.04 | 2.09 | 3.18 | -4.18 | -1.68 | 4.25 | 1.88 | 2.76 | -5.90 |
Vacuole | -3.02 | -1.08 | -2.97 | -1.43 | -2.60 | -6.94 | 4.99 | 1.33 | 7.64 | -3.50 | -7.36 | 3.39 | -1.37 | 5.54 | -3.78 |
Vacuole Periphery | 0.49 | 2.09 | -3.24 | -3.42 | -3.81 | -4.01 | 2.44 | -2.12 | -0.50 | -2.47 | -3.10 | 3.15 | -3.45 | -2.41 | -4.05 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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