Standard name
Human Ortholog
Description Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Cytoplasm 0.97 1.0 0.98 0.98 0.98 0.94 0.93 0.9 0.81 0.84 0.73 0.73 0.99 0.97 0.99 0.96 0.94 0.97 0.96 0.85 0.86 0.85 0.9 0.7
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Mitochondria 0 0 0.09 0.09 0 0.22 0.2 0.26 0.45 0.41 0.52 0.53 0 0 0 0 0 0 0 0 0.08 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 2 1 0 0 0 0 0 0 5
Bud 0 0 7 6 4 8 3 8 8 11 7 7 0 3 9 0 0 0 0 0 0 0 0 9
Bud Neck 0 0 0 0 2 1 1 3 1 3 0 3 0 0 0 0 0 1 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 1 2 0 1 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 1 6 2 8 3 4 7 2 5 0 2 5 20 14 16 0 0 0 0 0 0
Cytoplasm 96 22 119 240 445 315 520 678 289 459 209 269 111 209 264 421 176 415 101 34 111 70 183 194
Endoplasmic Reticulum 2 1 0 0 1 0 1 1 0 1 0 1 1 1 1 21 11 18 0 0 0 0 1 4
Endosome 0 0 0 0 3 0 1 1 0 0 0 1 0 0 0 9 3 2 0 0 2 0 0 13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 12
Mitochondria 2 0 11 21 17 75 111 194 160 221 148 196 2 3 12 15 3 9 1 0 10 2 3 14
Nucleus 0 0 0 2 0 1 0 5 1 1 0 1 1 0 2 1 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5
SpindlePole 0 0 0 0 1 0 1 4 2 2 0 1 0 1 0 5 1 1 0 0 0 0 0 2
Vac/Vac Membrane 2 0 0 0 0 2 2 2 0 2 0 0 0 0 1 3 7 7 0 1 2 3 6 8
Unique Cell Count 99 22 122 246 455 335 559 755 358 544 287 371 112 215 268 439 188 430 105 40 129 83 203 279
Labelled Cell Count 103 23 137 270 480 405 649 902 467 709 367 488 115 220 294 497 217 471 105 40 129 83 203 279


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.6 3.8 4.3 4.3 3.8 3.4 3.4 2.9 3.0 3.1 2.8 6.0 5.9 4.9 5.9 6.5 7.0 5.8 5.7 6.1
Std Deviation (1e-4) 0.6 0.9 0.5 1.0 0.8 0.9 1.0 0.9 0.9 0.8 0.9 0.8 1.0 1.0 1.0 1.0 1.4 1.1 1.3 1.1 1.2
Intensity Change (Log2) – – – 0.16 0.18 -0.02 -0.19 -0.16 -0.42 -0.33 -0.33 -0.44 0.66 0.62 0.36 0.63 0.75 0.86 0.61 0.57 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 -1.1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1 0
Cytoplasm 0 0.2 -1.5 -1.9 -2.8 -4.5 -3.9 -5.7 -5.9 0.9 -0.2 0.7 -0.8 -1.6 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -2.4 -3.4 -1.8 -2.6 -3.1 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7197 2.2436 1.7676 1.4613 1.4114 1.8177 2.288 2.818 2.6628 2.3098 2.1012 2.5944 0.218 0.7222 0.3376 0.6954 0.1758 0.4553
Actin 0.0526 0.0011 0.012 0.0242 0.015 0.0085 0.0137 0.0001 0.0007 0.001 0.0109 0.0042 0.0519 0.0005 0.0068 0.0112 0.0001 0.0078
Bud 0.001 0.0035 0.0007 0.0028 0.0002 0.0005 0.0025 0.0001 0.0004 0.0004 0.0005 0.003 0.006 0.0001 0.0008 0.0074 0.0001 0.0003
Bud Neck 0.0072 0.0004 0.0017 0.0016 0.0005 0.0041 0.0029 0.0001 0.0006 0.0004 0.0011 0.0013 0.0195 0.0001 0.0004 0.0003 0.0002 0.01
Bud Periphery 0.0018 0.0025 0.001 0.0024 0.0003 0.0005 0.0017 0 0.0002 0.001 0.0005 0.0026 0.0018 0.0001 0.0029 0.002 0.0001 0.0002
Bud Site 0.0021 0.026 0.0085 0.0016 0.0003 0.0004 0.0131 0.0003 0.0016 0.0004 0.0019 0.0009 0.008 0.0008 0.0016 0.0039 0 0.0004
Cell Periphery 0.0014 0.002 0.0013 0.0003 0.0002 0.0003 0.0006 0.0001 0.0001 0.0002 0.0002 0.0003 0.0005 0.0003 0.0003 0.0004 0.0001 0.0002
Cytoplasm 0.664 0.7884 0.7501 0.7734 0.7772 0.8349 0.5595 0.8683 0.8109 0.8028 0.9079 0.7933 0.478 0.7516 0.6614 0.5786 0.7823 0.6998
Cytoplasmic Foci 0.0283 0.014 0.0114 0.0336 0.0181 0.0268 0.0231 0.0041 0.0228 0.0078 0.0073 0.0113 0.0112 0.0077 0.0134 0.0331 0.011 0.009
Eisosomes 0.0008 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0 0.0007 0.0001 0.0003 0.0001 0 0.0001
Endoplasmic Reticulum 0.0154 0.0051 0.007 0.006 0.0049 0.0049 0.0251 0.0013 0.0038 0.0035 0.0041 0.0015 0.0054 0.0017 0.0035 0.0039 0.002 0.0046
Endosome 0.0317 0.0146 0.014 0.0408 0.0241 0.0259 0.0359 0.0019 0.0205 0.0096 0.0123 0.0237 0.0195 0.0034 0.0116 0.0363 0.0072 0.0104
Golgi 0.0095 0.0014 0.0029 0.0155 0.0086 0.0034 0.0084 0.0001 0.0045 0.001 0.0152 0.0094 0.0041 0.0007 0.0046 0.0026 0.0003 0.0064
Lipid Particles 0.0081 0.0026 0.0036 0.0072 0.0129 0.0022 0.0087 0.0001 0.003 0.0019 0.0021 0.0011 0.0022 0.0002 0.0047 0.0058 0.0001 0.0026
Mitochondria 0.0038 0.0005 0.0109 0.0028 0.0015 0.0016 0.0044 0.0001 0.0012 0.0102 0.007 0.0171 0.0097 0.0002 0.0071 0.0011 0.0001 0.0041
None 0.1265 0.1296 0.1532 0.0632 0.1138 0.0719 0.2216 0.1215 0.1143 0.1436 0.0244 0.1123 0.3649 0.2298 0.2581 0.2602 0.1938 0.2309
Nuclear Periphery 0.0125 0.0006 0.003 0.0017 0.0058 0.0039 0.0216 0.0002 0.0032 0.0049 0.0006 0.0007 0.0011 0.0002 0.0006 0.0011 0.0003 0.0014
Nucleolus 0.0008 0.0002 0.0003 0.0001 0.0002 0.0003 0.0012 0 0.0002 0.0003 0.0001 0.0011 0.0014 0.0001 0.0002 0.001 0 0.0001
Nucleus 0.0062 0.0011 0.0014 0.0016 0.0011 0.0017 0.0206 0.0004 0.0019 0.0013 0.0007 0.0017 0.0029 0.0005 0.0007 0.0019 0.0005 0.0009
Peroxisomes 0.0093 0.0002 0.0089 0.0111 0.0036 0.0008 0.0072 0 0.003 0.0002 0.0007 0.0016 0.0021 0.0001 0.0163 0.0373 0.0001 0.0085
Punctate Nuclear 0.0064 0.0004 0.0013 0.0014 0.0081 0.0037 0.0193 0.0001 0.0034 0.0003 0.0002 0.0003 0.0016 0.0002 0.0004 0.0047 0.0002 0.0005
Vacuole 0.009 0.0051 0.0053 0.0069 0.0028 0.0031 0.0072 0.001 0.003 0.0053 0.0018 0.0113 0.0062 0.0014 0.0039 0.0059 0.0013 0.0014
Vacuole Periphery 0.0018 0.0006 0.0012 0.0017 0.0007 0.0006 0.0017 0.0001 0.0005 0.0038 0.0005 0.0013 0.0012 0.0001 0.0005 0.0013 0.0002 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.375 21.4197 23.0468 28.1822 17.6043 13.1713 23.1012 21.1951 20.792 27.6221
Translational Efficiency 0.9518 0.6695 0.594 0.4966 0.7343 0.9877 0.5832 0.5966 0.6785 0.5853

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bul2

Bul2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bul2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available