Standard name
Human Ortholog
Description Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0 0.05 0.05 0 0 0.05 0 0 0 0.09 0.1 0.09 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.27 0 0 0 0.06 0.07 0.08 0.33 0.13 0.26 0.2 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.95 0.94 0.95 0.92 0.95 0.85 0.93 0.9 0.8 0.84 0.83 0.87 0.96 0.88 0.85 0.85 0.87 0.86 0.96 0.94 0.92 0.82 0.86 0.71
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0.09 0 0 0.11 0.08 0.16 0.1 0 0 0 0 0 0 0 0 0 0 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0.06 0.06 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 0 0 1 2 2 2 0 0 0 1 1 1 0 1 0 5 0 1
Bud Neck 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 1 1 0 10 5 2 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 4 8 12 9 5 9 9 3 15 2 2 5 24 31 26 15 10 0 0 3 1 1 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 6 4 5 0 0 0 0 1 0
Endosome 0 0 0 1 2 0 0 0 0 0 0 1 0 2 3 2 0 2 0 2 2 1 0 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 1 0 0
Mitochondria 4 92 1 1 1 7 12 17 90 38 57 59 2 4 3 13 2 2 0 1 0 1 2 4
Nucleus 69 319 200 155 258 93 170 199 218 237 182 259 162 247 260 241 213 195 86 318 203 156 196 162
Nuclear Periphery 0 0 0 0 1 0 0 3 5 4 1 1 0 2 2 4 2 2 0 0 0 1 1 0
Nucleolus 4 5 0 2 3 10 6 8 31 23 36 31 3 3 7 4 8 5 0 1 1 3 6 16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 0 0 3 0 2 0 3 0 3 6 2 0 1 1 0 0 1 1 2
Vac/Vac Membrane 0 2 2 2 4 1 2 4 2 3 4 3 0 3 4 8 16 14 0 8 3 11 14 30
Unique Cell Count 73 340 211 168 273 109 183 221 272 282 220 297 169 280 305 283 245 226 90 338 221 190 229 230
Labelled Cell Count 79 422 213 173 281 117 200 246 360 329 287 372 172 290 319 307 261 238 90 338 221 190 229 230


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 5.2 7.9 7.7 8.0 6.1 6.1 6.5 5.4 6.1 5.2 5.6 6.8 7.3 7.2 6.5 8.4 8.5 7.6 8.3 8.4
Std Deviation (1e-4) 1.1 0.8 1.3 1.7 1.5 1.3 1.2 1.4 1.5 1.8 1.0 1.1 1.4 1.5 1.8 1.8 2.4 2.6 1.6 1.5 1.7
Intensity Change (Log2) – – – -0.03 0.02 -0.38 -0.36 -0.27 -0.54 -0.37 -0.6 -0.5 -0.22 -0.11 -0.13 -0.27 0.09 0.11 -0.05 0.08 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 -0.3 0 0.5 0.2 0 0.8 0 0 -0.4 2.1 2.7 2.3 1.1 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.4 3.8 9.1 5.3 7.7 6.7 0 0 0 2.7 0 0
Nucleus -1.0 -0.1 -2.9 -0.8 -1.9 -4.7 -3.7 -3.9 -2.9 0.5 -2.5 -3.4 -3.4 -2.9 -3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 5.1 4.2 6.1 4.8 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.4747 6.1738 6.6766 5.8774 5.6319 6.3118 5.7097 6.8806 5.8929 6.8236 5.496 5.8395 2.8806 3.4857 3.0929 3.2214 2.223 2.9407
Actin 0.023 0.0001 0.0131 0.0001 0.004 0.0004 0.0021 0.0009 0.0011 0.0435 0.0001 0.0115 0.0424 0 0.0061 0 0.0009 0.0017
Bud 0.0005 0 0.0003 0 0.0009 0.0002 0.0001 0.0001 0.0001 0.0003 0 0.0001 0.0005 0 0.0001 0 0.0001 0.0001
Bud Neck 0.0034 0.0001 0.0016 0.0001 0.0008 0.0016 0.0002 0.0001 0.0023 0.0001 0.0002 0.0006 0.0032 0 0.0042 0.0001 0.002 0.0009
Bud Periphery 0.0005 0 0.0006 0 0.0023 0.0007 0.0003 0.0001 0.0003 0.0004 0.0001 0.0001 0.0014 0 0.0002 0 0.0001 0.0003
Bud Site 0.0063 0.0004 0.002 0 0.0009 0.0004 0.0002 0.0005 0.0128 0.0005 0.0001 0.0001 0.0045 0 0.0007 0 0.0002 0.0001
Cell Periphery 0.0001 0 0.0004 0 0.0002 0.0002 0.0001 0 0.0007 0 0 0 0.0007 0 0.0002 0 0 0.0001
Cytoplasm 0.0045 0.0061 0.0008 0.0002 0.0022 0.0002 0.0048 0.0314 0.0015 0.0001 0.0246 0.0074 0.0113 0 0.0001 0 0.0008 0.0004
Cytoplasmic Foci 0.0133 0.0002 0.0036 0.0088 0.0046 0.0004 0.0161 0.0064 0.0004 0.0044 0.0111 0.0041 0.0152 0 0.0017 0.0016 0.013 0.0015
Eisosomes 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0.0002 0 0 0.0004 0 0 0 0 0
Endoplasmic Reticulum 0.0013 0.0008 0.0006 0 0.0016 0.0002 0.0044 0.0002 0.0007 0.0002 0.0031 0.0005 0.0029 0 0.0002 0 0.0006 0.0007
Endosome 0.0149 0.0003 0.0048 0.0002 0.0046 0.0017 0.0244 0.0016 0.0029 0.0064 0.0234 0.0044 0.0226 0 0.005 0.0022 0.0292 0.0061
Golgi 0.0106 0 0.0042 0.0003 0.0016 0.0007 0.0023 0.0018 0.0002 0.0251 0.001 0.0014 0.0065 0 0.0076 0.0017 0.0153 0.0027
Lipid Particles 0.0099 0.0001 0.0027 0.0005 0.002 0.001 0.0164 0.0054 0.0003 0.0059 0.0009 0.0034 0.0172 0 0.0091 0.0142 0.0156 0.0027
Mitochondria 0.0048 0.0001 0.0039 0.0002 0.0052 0.0073 0.008 0.0003 0.0004 0.025 0.0007 0.0007 0.0146 0.0001 0.0026 0.0012 0.0014 0.0033
None 0.0019 0.0001 0.0002 0.0001 0.0002 0 0.0051 0.0008 0.0012 0.0002 0.0093 0.0029 0.0123 0 0 0 0.0001 0.0002
Nuclear Periphery 0.0052 0.0019 0.0033 0.0013 0.0098 0.0029 0.0548 0.0004 0.0075 0.0006 0.0015 0.0007 0.0088 0.0003 0.0021 0.0008 0.0017 0.0033
Nucleolus 0.0072 0.0054 0.007 0.0039 0.0209 0.0273 0.0051 0.0048 0.0083 0.0011 0.0233 0.0145 0.0092 0.0048 0.0041 0.0066 0.0284 0.0233
Nucleus 0.8604 0.9835 0.9435 0.95 0.8789 0.9493 0.8463 0.9301 0.9572 0.8734 0.8984 0.9349 0.796 0.9947 0.9534 0.9674 0.8863 0.9461
Peroxisomes 0.0103 0 0.0035 0.0336 0.0471 0.0012 0.0021 0.0129 0.0001 0.012 0.0003 0.0021 0.0082 0 0.0014 0.0031 0.0021 0.0002
Punctate Nuclear 0.0194 0.0006 0.0022 0.0003 0.0007 0.0004 0.0033 0.0022 0.001 0.0003 0.0012 0.0101 0.0092 0.0001 0.0003 0.0002 0.0008 0.0043
Vacuole 0.0015 0.0003 0.0012 0.0002 0.0081 0.003 0.0011 0.0001 0.0007 0.0001 0.0005 0.0002 0.0083 0 0.0006 0.0002 0.0007 0.001
Vacuole Periphery 0.0006 0 0.0004 0.0001 0.0032 0.0008 0.0028 0 0.0003 0.0002 0.0003 0.0001 0.0046 0 0.0003 0.0004 0.0006 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.0031 8.8564 4.9288 13.2954 9.6338 12.377 13.1521 15.4872 11.6333 13.4311
Translational Efficiency 1.0436 2.0107 2.7234 0.8241 1.1516 1.1209 1.5129 1.0566 1.2465 0.9352

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1877 911 1807 99 1720 1872 331 1624 3597 2783 2138 1723

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 877.30 935.20 1019.82 1192.06 773.92 947.49 1158.71 1087.57 827.87 943.47 1041.32 1093.57
Standard Deviation 119.89 178.32 151.37 154.96 115.02 125.18 131.48 170.02 128.43 144.85 156.74 170.93
Intensity Change Log 2 — 0.092205 0.217172 0.442315 — 0.291926 0.582263 0.490852 — 0.189263 0.399823 0.465267

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000620 0.000753 0.000462 0.000906 0.000270 0.000373 0.001190 0.000657 0.000453 0.000497 0.000574 0.000672
Bud Neck 0.004018 0.013897 0.019367 0.015781 0.005319 0.009320 0.011166 0.020618 0.004640 0.010818 0.018098 0.020340
Bud Site 0.000507 0.002828 0.000932 0.001401 0.000533 0.001352 0.001086 0.002347 0.000520 0.001835 0.000956 0.002293
Cell Periphery 0.000123 0.000338 0.000056 0.000079 0.000163 0.000140 0.000048 0.000113 0.000142 0.000205 0.000054 0.000111
Cytoplasm 0.001591 0.001697 0.000338 0.001183 0.003192 0.001480 0.000254 0.002365 0.002357 0.001551 0.000325 0.002297
Cytoplasmic Foci 0.000223 0.000301 0.000007 0.000017 0.000132 0.000940 0.000022 0.000049 0.000179 0.000731 0.000009 0.000047
Eisosomes 0.000022 0.000036 0.000035 0.000036 0.000019 0.000028 0.000061 0.000027 0.000020 0.000031 0.000039 0.000028
Endoplasmic Reticulum 0.005241 0.007679 0.002517 0.003182 0.004349 0.005533 0.005739 0.003693 0.004815 0.006236 0.003016 0.003663
Endosome 0.000324 0.000580 0.000076 0.000151 0.000161 0.000879 0.000105 0.000228 0.000246 0.000781 0.000080 0.000223
Golgi 0.000298 0.000185 0.000031 0.000047 0.000061 0.000566 0.000060 0.000101 0.000184 0.000442 0.000036 0.000098
Lipid Particles 0.000191 0.000164 0.000017 0.000054 0.000093 0.000448 0.000085 0.000025 0.000145 0.000355 0.000027 0.000027
Mitochondria 0.001230 0.001530 0.001103 0.001740 0.000573 0.004160 0.001163 0.001591 0.000916 0.003299 0.001113 0.001600
Mitotic Spindle 0.001745 0.002637 0.000117 0.001004 0.000838 0.003122 0.000076 0.001485 0.001311 0.002963 0.000110 0.001457
None 0.001799 0.005378 0.000768 0.002990 0.004032 0.002118 0.000866 0.001858 0.002867 0.003185 0.000783 0.001923
Nuclear Periphery 0.000357 0.000318 0.000426 0.000403 0.000207 0.000483 0.000203 0.000511 0.000285 0.000429 0.000392 0.000504
Nuclear Periphery Foci 0.000381 0.000239 0.000035 0.000095 0.000144 0.000458 0.000024 0.000093 0.000268 0.000386 0.000033 0.000093
Nucleolus 0.007287 0.010729 0.003742 0.002918 0.008122 0.009604 0.006980 0.003859 0.007686 0.009972 0.004243 0.003805
Nucleus 0.972646* 0.948983* 0.969233* 0.966162* 0.971079* 0.955914* 0.968743* 0.958184* 0.971896* 0.953645* 0.969157* 0.958643*
Peroxisomes 0.000569 0.000425 0.000100 0.000188 0.000139 0.000727 0.000453 0.000091 0.000363 0.000628 0.000155 0.000097
Vacuole 0.000599 0.000943 0.000540 0.001570 0.000450 0.001585 0.001591 0.001571 0.000528 0.001375 0.000702 0.001571
Vacuole Periphery 0.000230 0.000360 0.000098 0.000095 0.000124 0.000771 0.000085 0.000534 0.000179 0.000637 0.000096 0.000509

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.36 0.25 -1.52 -2.77 -4.20 -2.17 -14.44 -4.24 -2.86 5.83 -0.26 -0.98 -1.21 -2.22 -0.75
Bud Neck -7.27 -14.26 -4.68 -1.82 0.00 -4.45 -7.75 -12.77 -9.33 -6.59 -8.44 -14.85 -14.06 -8.83 -2.74
Bud Site -2.65 -4.52 -3.18 0.13 -2.38 -3.41 -7.45 -3.61 -1.73 -1.74 -4.07 -6.52 -3.87 -0.99 -2.67
Cell Periphery -1.42 3.19 0.80 1.58 -2.06 0.56 3.66 1.55 1.08 -2.30 -1.17 4.81 1.32 1.79 -2.43
Cytoplasm -0.08 2.54 -0.40 -0.37 -1.50 1.46 3.98 0.84 -0.59 -3.09 0.97 4.72 -0.01 -0.78 -3.27
Cytoplasmic Foci -0.32 2.71 2.35 1.50 -2.32 -2.70 2.09 1.70 3.06 -0.87 -2.56 3.50 2.76 3.34 -1.80
Eisosomes -5.05 -6.22 -3.17 -1.71 -1.69 -4.65 -14.79 -6.71 -0.11 11.58 -5.84 -12.56 -5.83 0.53 6.78
Endoplasmic Reticulum -3.47 8.90 2.07 4.41 -2.53 -3.46 -4.06 2.13 5.24 5.44 -4.30 7.99 4.01 7.20 -2.23
Endosome -0.98 3.42 -0.08 0.80 -1.57 -2.94 0.90 -0.89 2.62 -1.21 -2.86 3.98 0.29 2.87 -2.14
Golgi 0.56 1.34 0.94 0.88 -2.41 -1.89 -1.05 -0.93 1.73 -0.40 -1.21 1.37 0.81 1.86 -1.62
Lipid Particles 0.37 2.87 0.85 0.49 -2.60 -2.06 -0.72 2.54 2.43 4.70 -1.74 3.21 3.30 2.73 0.22
Mitochondria -0.56 0.10 -1.50 -1.36 -2.00 -3.76 -6.36 -6.20 2.45 -2.36 -3.42 -0.95 -2.94 2.22 -2.89
Mitotic Spindle -0.58 1.73 -0.84 -0.58 -1.22 -2.06 -0.20 -1.61 0.53 -1.13 -1.82 1.61 -1.42 0.31 -2.65
None -2.44 3.18 -1.15 1.67 -2.71 2.51 5.01 3.13 0.59 -2.79 -0.52 6.06 2.11 2.08 -3.56
Nuclear Periphery 0.51 -0.39 -1.73 -1.88 -0.96 -2.80 -2.23 -3.73 -1.02 -3.06 -2.07 -0.74 -3.24 -1.65 -1.16
Nuclear Periphery Foci 0.74 1.87 0.28 -0.21 -0.94 -1.60 2.46 0.73 1.78 -1.69 -0.81 2.28 1.53 1.95 -1.53
Nucleolus -3.00 4.69 3.61 5.36 0.65 -1.48 1.06 4.20 5.12 2.54 -2.99 5.60 4.77 6.56 0.76
Nucleus 5.60 2.69 2.61 0.19 1.94 4.39 2.86 5.95 0.86 3.36 6.74 3.37 6.98 0.48 4.77
Peroxisomes 0.41 1.20 0.81 1.75 -2.29 -3.39 -6.71 2.13 3.61 7.60 -1.10 0.97 1.29 4.35 4.22
Vacuole -1.87 -0.56 -1.92 -1.79 -1.87 -2.46 -6.09 -3.52 -0.26 1.15 -2.68 -2.40 -3.83 -1.23 -2.51
Vacuole Periphery -1.11 1.96 1.31 2.11 -1.11 -2.81 0.74 -1.50 0.97 -1.64 -2.85 1.94 -1.24 0.74 -1.62
Standard name
Human Ortholog
Description Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ctk3

Ctk3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ctk3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available