Standard name
Human Ortholog
Description TFIID subunit (65 kDa); involved in RNA polymerase II transcription initiation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0.09 0 0.05 0 0.05 0.05 0.06 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.13 0.05 0.05 0 0.05 0.07 0 0.08 0 0.07 0.12 0.16 0.06 0.05 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0.07 0.47 0.35 0.38 0.54 0.72 0.6 0.67 0.73 0.63 0.72 0.07 0 0 0.08 0 0.07 0 0 0 0 0 0
Nucleus 0.9 0.89 0.91 0.78 0.74 0.69 0.63 0.74 0.54 0.76 0.59 0.61 0.88 0.83 0.8 0.85 0.83 0.8 0.75 0.82 0.84 0.72 0.72 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.31 0.2 0.13 0 0.21 0.1 0.2 0.17 0.11 0.15 0.14 0.25 0.13 0.13 0.1 0.1 0.08 0.13 0.16 0.1 0.08 0.2 0.18 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 0 2 1 1 0 1 0 0 0 1 0 1 0 3 0 2 0 6
Bud Neck 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 1 3
Bud Site 5 1 1 2 1 3 3 8 5 7 3 2 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 3 7 2 3 2 3 3 8 7 1 4 1 29 56 69 34 18 24 0 0 0 0 2 4
Endoplasmic Reticulum 0 2 0 0 0 0 0 0 0 0 0 0 1 0 1 7 5 8 0 1 0 0 1 4
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 2 4 10 9 1 5 4 0 0 8 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 5 0 1 2 0
Mitochondria 60 32 42 8 16 32 71 96 64 91 31 51 26 13 12 45 17 19 1 3 0 2 3 4
Nucleus 269 398 82 18 31 41 62 118 51 94 29 43 346 393 351 455 320 207 221 377 82 188 366 258
Nuclear Periphery 1 6 0 1 0 1 2 0 3 5 1 1 1 1 1 7 7 1 0 0 0 0 1 1
Nucleolus 94 91 12 1 9 6 20 27 10 19 7 18 51 62 44 51 30 34 48 44 8 51 89 50
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 1 1 0 0 0 0 0 0 0
SpindlePole 1 2 0 1 1 0 1 6 0 0 0 0 4 4 2 21 14 4 3 2 1 2 7 4
Vac/Vac Membrane 1 5 0 0 0 1 0 0 0 0 0 0 3 3 0 9 12 5 8 17 1 8 19 23
Unique Cell Count 300 445 90 23 42 59 98 160 95 124 49 71 395 476 440 534 385 259 296 462 98 262 508 375
Labelled Cell Count 434 545 139 34 62 87 162 265 141 218 75 117 461 537 489 641 434 307 296 462 98 262 508 375


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.6 4.3 5.2 4.2 3.9 3.3 3.7 3.6 3.5 3.7 3.6 6.4 6.2 6.2 6.6 8.3 7.9 5.8 5.9 6.5
Std Deviation (1e-4) 0.9 1.2 0.6 2.7 1.4 1.2 0.8 1.1 1.0 0.9 1.8 1.0 1.5 1.6 1.7 1.6 2.0 1.9 1.2 1.3 1.2
Intensity Change (Log2) – – – 0.3 -0.03 -0.11 -0.35 -0.19 -0.23 -0.29 -0.19 -0.25 0.58 0.54 0.55 0.64 0.96 0.9 0.44 0.46 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 1.8 2.7 3.4 1.6 0 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.0 0 0 0 0 0 0 0 0 -9.9 -12.9 -12.6 -9.7 -10.9 -8.5
Nucleus -1.7 -2.6 -3.4 -4.5 -3.3 -5.7 -2.9 -4.5 -4.6 -0.9 -2.0 -2.5 -1.5 -1.9 -2.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 -0.1 -0.1 -0.9 -1.1 -1.7 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0003 0 0.0001 0 0 0 0.0025 0.0024 0.0167 0 0.0071 0.0064 – – – – – –
Bud 0.0001 0 0.0001 0 0 0 0.0006 0 0.0013 0 0.0001 0.0004 – – – – – –
Bud Neck 0.001 0.0001 0.0003 0.0001 0.0004 0.002 0.0031 0.0004 0.0014 0 0.0005 0.0033 – – – – – –
Bud Periphery 0.0002 0 0.0001 0.0001 0 0 0.0012 0 0.0012 0 0.0002 0.0005 – – – – – –
Bud Site 0.0003 0.0001 0.004 0.0001 0.0001 0.0001 0.0032 0.0008 0.0029 0 0.0008 0.0012 – – – – – –
Cell Periphery 0.0001 0 0.0001 0 0 0.0001 0.0004 0 0.0001 0 0.0001 0.0001 – – – – – –
Cytoplasm 0.001 0.0001 0.0111 0.0001 0.0001 0 0.0077 0.0099 0.0029 0 0.0019 0.0006 – – – – – –
Cytoplasmic Foci 0.0008 0 0.0042 0 0 0 0.0117 0.0047 0.0019 0 0.0128 0.0021 – – – – – –
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0 – – – – – –
Endoplasmic Reticulum 0.001 0 0.0003 0 0 0 0.004 0.0008 0.0003 0 0.0059 0.0004 – – – – – –
Endosome 0.0039 0 0.0068 0 0 0 0.0123 0.001 0.0035 0 0.0327 0.0021 – – – – – –
Golgi 0.0002 0 0.0008 0 0 0 0.002 0.0004 0.0043 0 0.0111 0.0017 – – – – – –
Lipid Particles 0.0015 0 0.0004 0 0 0 0.01 0.0004 0.0011 0 0.0453 0.0007 – – – – – –
Mitochondria 0.0006 0.0002 0.0002 0.0005 0.0001 0.0001 0.0046 0.0013 0.0069 0.0001 0.0058 0.0025 – – – – – –
None 0.0004 0 0.0002 0 0 0 0.0083 0.0011 0.0002 0 0.0009 0.0003 – – – – – –
Nuclear Periphery 0.0357 0.0003 0.0006 0.0005 0.0004 0.0001 0.0291 0.0008 0.0019 0.0013 0.0031 0.0026 – – – – – –
Nucleolus 0.046 0.0247 0.028 0.0152 0.1183 0.0896 0.0598 0.0191 0.0192 0.0115 0.0806 0.1027 – – – – – –
Nucleus 0.8849 0.9741 0.9402 0.9831 0.8783 0.9067 0.8181 0.9551 0.9303 0.9869 0.7632 0.8611 – – – – – –
Peroxisomes 0.0001 0 0.0001 0 0 0 0.0025 0.0002 0.0019 0 0.0244 0.0034 – – – – – –
Punctate Nuclear 0.0194 0.0003 0.0003 0.0001 0.002 0.001 0.0129 0.0012 0.0009 0.0001 0.0017 0.0072 – – – – – –
Vacuole 0.0015 0.0001 0.0018 0.0001 0.0001 0.0002 0.0042 0.0001 0.0005 0 0.001 0.0005 – – – – – –
Vacuole Periphery 0.001 0 0.0002 0.0001 0 0 0.0019 0.0001 0.0004 0 0.0007 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.8733 18.016 15.7559 21.1454 15.8646 5.755 20.8182 17.7832 19.253 20.4726
Translational Efficiency 0.6768 0.8207 0.9242 0.801 0.8346 2.1747 0.6388 0.9001 0.7868 0.7815

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2537 1079 98 1702 1831 1304 116 135 4368 2383 214 1837

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 785.31 854.78 1065.59 1015.84 813.26 852.50 1153.52 1103.19 797.03 853.53 1113.25 1022.26
Standard Deviation 95.14 134.74 163.26 143.16 97.81 118.82 135.80 160.90 97.25 126.28 155.31 146.33
Intensity Change Log 2 — 0.122291 0.440318 0.371339 — 0.067983 0.504254 0.439893 — 0.094918 0.473199 0.406622

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000163 0.000992 0.002232 0.001477 0.000280 0.000699 0.002355 0.001369 0.000212 0.000831 0.002299 0.001469
Bud Neck 0.008109 0.013621 0.013220 0.043063 0.006052 0.009519 0.013118 0.020789 0.007247 0.011376 0.013165 0.041426
Bud Site 0.000542 0.002718 0.002131 0.010732 0.000440 0.001461 0.002267 0.004861 0.000499 0.002030 0.002204 0.010301
Cell Periphery 0.000058 0.000159 0.000067 0.000155 0.000037 0.000047 0.000076 0.000151 0.000049 0.000098 0.000072 0.000155
Cytoplasm 0.003548 0.002286 0.003414 0.004811 0.000719 0.002332 0.000642 0.013811 0.002362 0.002311 0.001912 0.005473
Cytoplasmic Foci 0.000641 0.000833 0.000241 0.001179 0.000167 0.001305 0.000067 0.001937 0.000442 0.001091 0.000147 0.001235
Eisosomes 0.000017 0.000036 0.000068 0.000041 0.000019 0.000030 0.000075 0.000043 0.000018 0.000033 0.000072 0.000041
Endoplasmic Reticulum 0.001478 0.004562 0.006349 0.003377 0.002333 0.003122 0.007364 0.002700 0.001836 0.003774 0.006899 0.003327
Endosome 0.000287 0.000706 0.000225 0.002012 0.000199 0.001052 0.000276 0.000298 0.000250 0.000895 0.000253 0.001886
Golgi 0.000355 0.000690 0.000221 0.001293 0.000096 0.001154 0.000206 0.000287 0.000247 0.000944 0.000213 0.001219
Lipid Particles 0.000385 0.000678 0.000264 0.000125 0.000301 0.001063 0.000243 0.000088 0.000350 0.000889 0.000253 0.000123
Mitochondria 0.001183 0.002588 0.001646 0.003802 0.000933 0.004001 0.001841 0.001895 0.001078 0.003361 0.001752 0.003662
Mitotic Spindle 0.001075 0.004923 0.007608 0.022620 0.003023 0.013023 0.000837 0.025458 0.001891 0.009355 0.003938 0.022828
None 0.004011 0.004694 0.001245 0.004305 0.001903 0.002526 0.001185 0.013974 0.003127 0.003508 0.001213 0.005015
Nuclear Periphery 0.000543 0.000462 0.000372 0.001809 0.000658 0.000553 0.000411 0.000523 0.000591 0.000512 0.000393 0.001715
Nuclear Periphery Foci 0.000191 0.000494 0.000951 0.001588 0.000290 0.000392 0.000204 0.000626 0.000232 0.000438 0.000546 0.001517
Nucleolus 0.058843 0.069568 0.044274 0.025965 0.057476 0.063948 0.056422 0.019372 0.058270 0.066493 0.050859 0.025480
Nucleus 0.917131* 0.887553* 0.909152* 0.865196* 0.923548* 0.891000* 0.907033* 0.889247* 0.919821* 0.889439* 0.908004* 0.866964*
Peroxisomes 0.000428 0.001207 0.002081 0.000485 0.000360 0.001195 0.001653 0.001024 0.000400 0.001201 0.001849 0.000524
Vacuole 0.000434 0.000787 0.003981 0.004016 0.000868 0.000912 0.003479 0.001347 0.000616 0.000855 0.003709 0.003820
Vacuole Periphery 0.000577 0.000446 0.000258 0.001948 0.000299 0.000667 0.000246 0.000199 0.000460 0.000567 0.000252 0.001819

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.40 -9.45 -15.00 -3.90 3.08 -3.58 -10.90 -9.34 -5.18 4.54 -7.29 -14.13 -15.64 -5.65 6.40
Bud Neck -5.85 -4.74 -22.61 -17.38 -11.12 -4.86 -6.52 -8.72 -7.15 -3.67 -7.14 -7.82 -24.10 -20.39 -13.53
Bud Site -3.15 -7.05 -6.49 -3.89 -4.08 -3.38 -7.63 -2.91 -2.50 -1.48 -4.43 -10.01 -6.98 -5.18 -3.64
Cell Periphery -2.40 -1.09 -3.86 0.79 -3.30 -1.81 -6.13 -5.25 -4.57 -0.49 -2.52 -4.74 -5.46 -1.20 -1.70
Cytoplasm 1.59 -0.11 -0.97 -2.49 -0.16 -2.03 -1.30 -1.67 -1.15 -1.50 -0.04 -0.09 -2.70 -2.50 -1.21
Cytoplasmic Foci -0.39 1.34 0.36 0.78 -1.04 -2.49 0.68 -0.99 -0.09 -1.03 -2.01 1.62 -0.55 1.35 -1.79
Eisosomes -11.71 -9.39 -20.98 -5.71 4.38 -6.97 -11.21 -7.91 -5.43 4.19 -12.82 -14.43 -23.15 -8.96 7.97
Endoplasmic Reticulum -13.23 -5.12 -14.52 2.39 2.10 -3.12 -7.70 -3.70 -1.50 5.66 -11.01 -9.24 -12.48 -0.37 5.92
Endosome -1.86 0.63 -3.16 -1.26 -3.32 -2.39 -3.36 -2.57 1.50 0.30 -2.92 -2.39 -3.40 -0.56 -2.71
Golgi -0.85 0.36 -0.89 -0.09 -1.38 -1.79 -5.41 -4.20 1.26 -0.93 -1.83 -0.38 -1.35 0.52 -1.30
Lipid Particles -0.48 1.18 1.85 1.24 1.18 -1.55 -1.26 1.27 1.89 3.90 -1.49 0.12 2.24 2.27 3.78
Mitochondria -2.39 -2.45 -7.01 -1.82 -5.32 -2.94 -6.72 -6.74 1.48 -0.61 -3.60 -5.60 -8.54 -0.39 -5.24
Mitotic Spindle -2.10 -0.95 -8.06 -5.86 -2.93 -3.74 -0.64 -2.33 -1.14 -2.01 -4.49 -1.23 -8.11 -4.60 -4.63
None -0.72 5.13 -0.33 0.40 -5.38 -1.98 2.50 -2.42 -2.14 -2.69 -0.79 5.66 -2.29 -1.72 -6.06
Nuclear Periphery 1.01 1.00 -3.80 -4.12 -3.98 0.61 -0.73 -0.85 -1.48 -0.10 0.81 -0.19 -3.63 -3.90 -3.42
Nuclear Periphery Foci -3.09 -1.09 -2.12 -1.43 -0.54 -1.28 -1.08 -0.76 0.20 0.02 -2.86 -1.40 -2.07 -1.46 -1.05
Nucleolus -2.85 0.88 12.24 12.26 2.82 -2.08 1.46 6.23 7.24 1.91 -3.34 1.74 14.64 15.68 3.17
Nucleus 5.71 1.62 10.17 4.09 2.58 6.35 2.90 2.87 0.75 0.88 8.25 3.09 11.56 4.62 3.50
Peroxisomes -2.23 -4.56 -0.10 2.40 4.78 -2.84 -5.79 -2.38 0.54 1.63 -3.54 -6.80 -0.92 3.37 6.89
Vacuole -1.67 -1.63 -5.44 -4.69 0.24 -0.15 -3.05 -1.86 -1.86 1.60 -1.03 -3.47 -5.24 -5.00 1.91
Vacuole Periphery 0.51 1.21 -1.04 -1.56 -2.09 -1.81 -0.40 0.49 2.15 1.78 -0.58 1.04 -1.43 -1.15 -2.02
Standard name
Human Ortholog
Description TFIID subunit (65 kDa); involved in RNA polymerase II transcription initiation
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Taf8

Taf8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Taf8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available