Standard name
Human Ortholog
Description Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex; subunit of Gtr1-Gtr2, a GTPase that activates TORC1 in response to amino acid stimulation; subunit of EGO/GSE, a vacuolar/endosomal membrane complex that regulates exit from rapamycin-induced growth arrest and sorting of Gap1p from the endosome to the plasma membrane; involved in phosphate transport and telomeric chromatin silencing; activated by the the Iml1p (GAP) subunit of the SEACIT complex; similar to human RagA and RagB
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.05 0 0.07 0.11 0.16 0.15 0.2 0.22 0.24 0.27 0.3 0 0 0.05 0.06 0.07 0.09 0.18 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.05 0 0 0.05 0.05 0 0 0 0 0 0 0.07 0 0 0 0 0 0.12 0.06 0.07 0.09 0.11 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.09 0 0 0 0 0 0
Mitochondria 0.28 0 0.1 0.08 0.08 0.25 0.36 0.21 0.49 0.34 0.5 0.27 0 0 0 0 0 0.07 0 0 0 0 0 0
Nucleus 0.08 0.11 0.1 0 0 0.05 0 0 0 0 0 0 0.09 0.05 0.07 0 0 0 0 0.06 0 0 0.05 0
Nuclear Periphery 0.11 0.14 0.24 0.13 0 0 0 0.05 0 0 0 0 0.07 0.05 0.09 0.2 0.12 0.16 0.07 0 0.16 0 0.06 0.06
Nucleolus 0.16 0.11 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.51 0.75 0.69 0.85 0.81 0.67 0.64 0.51 0.51 0.54 0.38 0.45 0.86 0.9 0.87 0.86 0.84 0.83 0.53 0.81 0.72 0.8 0.72 0.72
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 8 0 0 0 3 0 0 1 0 0 2 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 3 0 3 15 8 0 0 0 0 0 0 0 0 1 0 0 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 3 11 9 4 4 6 6 0 0 1 1 2 0 0 0 0 0 0 0
Cytoplasm 34 4 1 20 41 61 76 88 81 104 93 108 12 20 26 8 15 20 33 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 1 5 2 8 36 8 7 7 12 2 3 3 4 9 8 0 0 0 0 0 1
Endosome 4 4 2 8 20 19 17 11 6 10 1 8 29 20 17 1 1 7 22 6 5 12 44 57
Golgi 0 1 0 5 14 5 2 13 0 2 0 3 1 1 3 10 14 20 0 1 0 0 1 2
Mitochondria 47 2 5 23 32 100 178 96 179 144 173 99 9 2 1 5 9 14 6 0 0 4 9 7
Nucleus 13 9 5 8 17 19 12 10 6 12 2 9 36 22 39 5 2 4 5 6 1 4 19 18
Nuclear Periphery 19 11 12 39 4 11 12 24 9 7 4 6 27 21 50 27 26 34 12 2 12 5 24 25
Nucleolus 27 9 4 2 2 2 0 1 0 0 0 0 18 15 9 4 8 8 4 1 0 1 3 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 2 2 0 1 0 0 1 0 0 2 2 2 0 1 1 0 0 0 0 3 3
Vac/Vac Membrane 86 59 34 252 311 263 315 228 188 229 130 163 346 407 471 114 183 176 99 85 55 118 287 339
Unique Cell Count 170 79 49 295 385 393 492 451 369 425 343 362 401 454 540 132 219 213 187 106 77 149 398 471
Labelled Cell Count 231 99 63 360 448 486 632 527 481 523 431 425 482 513 623 179 270 294 187 106 77 149 398 471


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.3 9.3 7.3 8.5 8.4 7.0 6.7 7.4 6.5 6.9 6.3 7.1 9.5 9.8 9.8 15.8 17.0 16.8 9.2 9.5 10.5
Std Deviation (1e-4) 9.3 2.1 1.3 1.7 1.5 1.6 1.3 1.3 1.2 1.2 1.2 1.3 1.7 1.7 1.7 4.1 4.7 4.5 2.0 2.0 2.6
Intensity Change (Log2) – – – 0.23 0.21 -0.04 -0.11 0.04 -0.16 -0.07 -0.2 -0.04 0.38 0.43 0.43 1.12 1.23 1.21 0.34 0.39 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 2.6 2.6 3.0 3.3 3.6 3.8 4.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.4 3.7 1.8 5.1 3.4 5.3 2.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.1 0 0 0 0 0 0 0 0 0 0 -3.3 -0.6 -2.3 -1.4
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.8 1.9 -0.3 -0.7 -2.5 -2.4 -2.1 -4.2 -3.2 3.1 4.1 3.4 2.6 2.3 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0059 3.4384 3.2791 3.0297 2.707 3.2001 3.1516 4.2493 3.892 3.5486 3.2139 3.8099 2.284 3.1778 2.8166 2.2688 2.0659 2.8571
Actin 0.0029 0 0.0087 0 0.0008 0 0.0151 0 0.0088 0 0.0084 0.0129 0.0124 0 0.0127 0.0152 0 0
Bud 0.0003 0.001 0.0004 0 0.0001 0.0002 0.0005 0.0002 0.0003 0.0004 0.0003 0.0001 0.0006 0.0003 0.0089 0.0004 0 0
Bud Neck 0.0004 0.0001 0.0002 0 0.0001 0.0001 0.0012 0.0001 0.0005 0.001 0.0003 0.0002 0.0007 0.0002 0.0019 0.0073 0 0
Bud Periphery 0.0017 0.0013 0.001 0.0002 0.0003 0.0006 0.0006 0.0003 0.0005 0.0006 0.0008 0.0002 0.0005 0.0004 0.0019 0.0006 0.0001 0.0003
Bud Site 0.0004 0.0006 0.0006 0 0.0001 0.0001 0.0023 0.0006 0.0013 0.0225 0.001 0.0001 0.0017 0.0006 0.0028 0.0154 0 0
Cell Periphery 0.0006 0.0004 0.0005 0.0001 0.0001 0.0002 0.0005 0.0005 0.0006 0.0005 0.0004 0.0003 0.001 0.0003 0.001 0.0005 0.0001 0.0001
Cytoplasm 0.003 0.0029 0.0018 0.0002 0.0014 0.0006 0.0098 0.0021 0.0013 0.0008 0.0007 0.0005 0.007 0.0064 0.0017 0.0007 0.0005 0.0001
Cytoplasmic Foci 0.0057 0.0017 0.0029 0.0004 0.0011 0.0014 0.02 0.003 0.0063 0.0009 0.0024 0.0049 0.0296 0.0037 0.0076 0.007 0.0002 0.0001
Eisosomes 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0003 0 0.0001 0 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0036 0.0026 0.0025 0.0006 0.0014 0.001 0.0036 0.0008 0.0039 0.0004 0.0019 0.0008 0.002 0.0009 0.001 0.0003 0.0008 0.0008
Endosome 0.0941 0.0599 0.1012 0.0673 0.092 0.0727 0.1021 0.0424 0.0761 0.0587 0.0825 0.0452 0.0945 0.0992 0.0717 0.1066 0.0609 0.016
Golgi 0.006 0.001 0.0036 0.001 0.0018 0.0021 0.0167 0.0009 0.0081 0.0009 0.0128 0.0067 0.0065 0.0025 0.0085 0.0032 0.0007 0.001
Lipid Particles 0.0162 0.0081 0.0057 0.0036 0.0025 0.0034 0.0241 0.0023 0.0076 0.0008 0.0087 0.014 0.0187 0.0059 0.0029 0.0046 0.0004 0.0038
Mitochondria 0.0253 0.0096 0.0164 0.001 0.0043 0.0059 0.0208 0.0027 0.0281 0.001 0.0421 0.0076 0.0229 0.0068 0.0365 0.0023 0.0009 0.0169
None 0.0088 0.0002 0.0003 0 0.0037 0.0001 0.0077 0.0002 0.0004 0.0002 0.0005 0.0002 0.0094 0.0002 0.0003 0.0001 0 0
Nuclear Periphery 0.0303 0.024 0.0147 0.0098 0.0195 0.0199 0.0158 0.0259 0.0123 0.0026 0.0107 0.0096 0.0204 0.005 0.0129 0.0023 0.0071 0.0405
Nucleolus 0.0069 0.0006 0.0012 0.0002 0.0015 0.0004 0.0043 0.0013 0.0011 0.0025 0.0009 0.0018 0.004 0.0018 0.0026 0.0006 0.0001 0.0002
Nucleus 0.0081 0.0036 0.0051 0.0005 0.0053 0.0014 0.0095 0.0056 0.0017 0.002 0.0017 0.0007 0.0027 0.0013 0.0018 0.0005 0.0004 0.0006
Peroxisomes 0.0036 0.0001 0.0005 0 0.0003 0.0007 0.0096 0.0002 0.0042 0.0002 0.0012 0.0026 0.0078 0.0003 0.0015 0.0067 0 0.0002
Punctate Nuclear 0.002 0.0001 0.0006 0 0.0037 0 0.0139 0.0002 0.0016 0.0001 0.0004 0.0003 0.003 0 0 0.0001 0 0
Vacuole 0.2971 0.3436 0.3303 0.3161 0.2656 0.2792 0.2849 0.4088 0.3533 0.3817 0.2979 0.2247 0.3161 0.3089 0.2853 0.3233 0.25 0.1667
Vacuole Periphery 0.4832 0.5386 0.5016 0.5988 0.5943 0.61 0.4368 0.5019 0.482 0.522 0.5241 0.6666 0.4383 0.5552 0.5363 0.5021 0.6777 0.7527

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9657 32.7735 17.2589 19.975 26.0669 16.5478 22.4478 26.3335 28.2449 24.5213
Translational Efficiency 1.1844 0.5385 0.9965 1.0367 0.7414 1.1393 0.8452 0.5869 0.7415 0.7379

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex; subunit of Gtr1-Gtr2, a GTPase that activates TORC1 in response to amino acid stimulation; subunit of EGO/GSE, a vacuolar/endosomal membrane complex that regulates exit from rapamycin-induced growth arrest and sorting of Gap1p from the endosome to the plasma membrane; involved in phosphate transport and telomeric chromatin silencing; activated by the the Iml1p (GAP) subunit of the SEACIT complex; similar to human RagA and RagB
Localization
Cell Percentages vacuolar membrane (97%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Gtr1

Gtr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gtr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available