Standard name
Human Ortholog
Description Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0 0.09 0.09 0.12 0.05 0 0 0.06 0.05 0.08 0.08 0.05 0.05 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.07 0 0.05 0 0 0.05 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0.06 0.05 0.08 0.09 0 0 0 0.06 0 0.05 0.08 0.11 0.15 0.19 0.28 0.28 0.12 0 0 0 0 0 0
Cytoplasm 0.23 0.23 0.25 0.2 0.43 0.55 0.58 0.64 0.59 0.6 0.58 0.53 0.08 0.1 0.1 0.62 0.64 0.94 0 0 0 0 0 0
Endoplasmic Reticulum 0.66 0.79 0.65 0.68 0.41 0.37 0.32 0.28 0.25 0.2 0.27 0.23 0.84 0.84 0.83 0 0 0 0.85 0.87 0.86 0.92 0.86 0.88
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.11 0.09 0.08 0.07 0.06 0.06 0.09 0.14 0.07 0.1 0 0 0 0.05 0.07 0 0 0 0.05 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 4 26 26 50 20 14 19 23 23 33 30 12 8 6 2 2 0 3 6 4 1 1 1
Bud 3 0 11 4 30 5 24 22 14 22 25 25 0 0 0 1 1 0 0 5 1 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 9 23 16 21 36 10 21 22 23 21 20 30 27 27 42 17 21 2 0 0 0 0 0 0
Cytoplasm 39 85 73 55 174 232 280 334 209 282 233 200 20 18 22 38 47 16 8 9 5 0 4 5
Endoplasmic Reticulum 110 295 189 187 168 158 152 146 89 94 107 86 208 147 180 2 2 0 176 344 298 122 187 163
Endosome 0 0 3 1 6 0 2 5 1 4 2 0 0 0 0 0 0 0 1 1 3 0 0 0
Golgi 1 2 5 11 2 0 0 0 0 0 0 0 3 3 1 2 4 0 0 0 0 0 2 0
Mitochondria 0 0 6 1 3 4 2 4 8 14 25 24 1 0 0 1 1 0 2 2 4 0 1 3
Nucleus 1 0 1 1 0 2 1 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 1 2 2 5 0 0 3 3 2 5 2 3 3 7 5 0 0 0 1 2 5 0 2 0
Nucleolus 2 1 0 0 1 0 3 0 0 3 0 1 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 7 5 33 24 34 31 31 33 31 65 28 37 7 2 1 3 5 0 6 14 17 4 10 6
Unique Cell Count 166 373 293 276 407 422 482 524 356 469 402 376 247 176 217 61 74 17 208 397 347 133 218 187
Labelled Cell Count 185 417 365 336 504 462 533 588 401 533 477 437 282 212 257 66 84 18 208 397 347 133 218 187


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.0 19.7 14.5 15.5 13.5 11.9 10.7 10.1 9.1 8.8 8.6 8.8 19.7 17.2 17.0 27.4 25.5 25.1 21.2 21.0 21.6
Std Deviation (1e-4) 4.8 3.9 4.0 2.7 2.3 2.2 2.1 1.8 1.9 2.1 1.8 2.2 3.6 2.9 2.9 6.3 7.9 6.4 4.7 4.2 5.0
Intensity Change (Log2) – – – 0.1 -0.1 -0.28 -0.44 -0.52 -0.67 -0.71 -0.74 -0.72 0.45 0.25 0.23 0.92 0.82 0.79 0.55 0.54 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.2 1.4 -2.2 -3.6 -3.2 -1.2 -2.2 -0.3 -0.4 -1.8 -1.8 -2.8 0 -1.8 0
Bud -1.7 2.0 -2.3 0.8 0.3 0.1 0.6 1.4 1.6 -3.1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.0 1.7 -2.2 -0.7 -0.8 0.5 -0.6 -0.3 1.3 2.3 3.6 4.9 5.5 5.9 0
Cytoplasm -1.4 4.9 8.0 9.0 10.6 8.6 9.5 8.7 7.4 -5.2 -3.9 -4.2 5.7 6.3 0
Endoplasmic Reticulum 0.8 -6.1 -7.1 -9.0 -10.2 -10.1 -12.4 -10.0 -10.9 5.2 4.4 4.6 -8.7 -9.5 -5.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1.6 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.2 0.8 2.6 2.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.0 -1.3 -1.8 -2.4 -2.5 -1.1 1.0 -2.0 -0.6 -3.7 -4.0 -4.8 -1.5 -1.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.4896 16.7715 16.7266 16.2795 14.9361 15.563 18.0287 18.0088 18.1966 16.9 18.8116 17.7136 20.8056 22.1424 21.0058 21.7658 20.746 20.7524
Actin 0.0002 0.0005 0.0001 0 0.0004 0.0004 0.0003 0.0001 0.0004 0.0004 0.0004 0.0003 0.0001 0.0001 0 0 0.0001 0.0002
Bud 0.0003 0.0001 0 0 0 0 0 0.0002 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.0002 0.0005 0.0001 0.0001 0.0003 0.0003 0 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0002 0.0002 0 0.0002 0.0002
Bud Periphery 0.0003 0.0001 0 0 0.0002 0 0.0001 0.0005 0 0 0 0 0 0 0 0 0 0
Bud Site 0.001 0.0006 0 0 0.0001 0 0 0.0064 0.0002 0 0 0 0 0.0001 0 0 0 0
Cell Periphery 0.0023 0.0095 0.0019 0.0009 0.0025 0.0011 0.0012 0.0036 0.0033 0.0017 0.0022 0.0018 0.0023 0.0086 0.0036 0.0009 0.0027 0.0014
Cytoplasm 0.0142 0.0333 0.0204 0.0029 0.0387 0.0101 0.0063 0.0126 0.0156 0.0047 0.0062 0.009 0.0057 0.0179 0.0135 0.001 0.0112 0.0024
Cytoplasmic Foci 0.0002 0.0012 0.0004 0.0003 0.0013 0.0002 0.0001 0.002 0.0006 0.0021 0.001 0.0005 0.0001 0.0002 0.0001 0 0.0003 0
Eisosomes 0 0.0001 0 0 0.0003 0.0001 0 0 0.0001 0 0.0001 0 0 0.0001 0 0 0 0
Endoplasmic Reticulum 0.9613 0.9011 0.9653 0.9652 0.9367 0.9728 0.9701 0.951 0.9617 0.9434 0.9663 0.974 0.9697 0.9528 0.9719 0.9954 0.9684 0.9835
Endosome 0.0006 0.0043 0.0011 0.001 0.0027 0.0018 0.0008 0.0014 0.0023 0.0036 0.0038 0.001 0.0005 0.0004 0.0002 0 0.0011 0.0005
Golgi 0.0001 0.0012 0.0002 0.0004 0.0007 0.0003 0.0001 0.0004 0.0014 0.0018 0.0033 0.0003 0.0001 0.0003 0.0001 0 0.0004 0.0016
Lipid Particles 0.002 0.013 0.0029 0.0245 0.0032 0.0011 0.0008 0.0124 0.0047 0.0383 0.0061 0.0051 0.0018 0.0121 0.0041 0.0004 0.0056 0.0012
Mitochondria 0.0001 0.0002 0 0 0.0003 0.0001 0.0001 0.0001 0.0002 0 0.0008 0 0 0 0 0 0 0
None 0.0004 0.0005 0.0002 0 0.0011 0.0001 0.0012 0.0006 0.0008 0 0.0002 0.0001 0.0002 0 0 0 0 0
Nuclear Periphery 0.0108 0.0185 0.0044 0.0028 0.0065 0.0074 0.0158 0.003 0.0055 0.0024 0.0068 0.0045 0.0156 0.0041 0.0035 0.0017 0.0042 0.0067
Nucleolus 0.0002 0.0002 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0
Nucleus 0.002 0.0056 0.0003 0.0002 0.0008 0.0006 0.0008 0.0004 0.0008 0.0001 0.0001 0.0003 0.0014 0.0001 0.0002 0 0.0002 0.0005
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Punctate Nuclear 0.0001 0.0008 0.0001 0 0.0004 0.0001 0.0001 0.0005 0.0002 0 0 0 0 0 0 0 0 0.0001
Vacuole 0.0033 0.0081 0.0023 0.0013 0.0037 0.0033 0.0017 0.0045 0.0018 0.001 0.0017 0.0024 0.0022 0.0028 0.0025 0.0006 0.0054 0.0016
Vacuole Periphery 0.0002 0.0004 0.0001 0.0001 0.0002 0.0002 0.0005 0.0001 0.0002 0.0001 0.0009 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 227.9664 212.1917 178.5014 106.0988 83.7952 197.3049 207.6431 191.4002 137.8898 118.3245
Translational Efficiency 1.0577 1.2616 1.0738 1.1919 1.3723 0.9938 1.1067 0.8335 0.9038 0.8708

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
376 1536 75 1308 1923 1610 203 1595 2299 3146 278 2903

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1301.13 1289.82 1756.53 2074.13 1397.60 1459.53 2088.29 2121.70 1381.82 1376.67 1998.79 2100.27
Standard Deviation 200.51 265.86 233.47 334.58 257.41 245.98 254.19 352.02 251.54 269.58 289.08 345.09
Intensity Change Log 2 — -0.012595 0.432963 0.672741 — 0.062552 0.579371 0.602269 — 0.026810 0.510635 0.636676

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000337 0.001368 0.001182 0.000749 0.000349 0.000734 0.000314 0.000650 0.000347 0.001043 0.000548 0.000695
Bud Neck 0.000271 0.001015 0.001294 0.003955 0.000320 0.000230 0.000418 0.001212 0.000312 0.000614 0.000654 0.002448
Bud Site 0.000722 0.010317 0.000443 0.007477 0.004253 0.002308 0.001025 0.003656 0.003675 0.006218 0.000868 0.005378
Cell Periphery 0.003549 0.004464 0.005816 0.013504 0.003114 0.003600 0.013182 0.013398 0.003185 0.004021 0.011195 0.013446
Cytoplasm 0.002850 0.027260 0.000192 0.010656 0.016945 0.002740 0.000601 0.008876 0.014640 0.014712 0.000491 0.009678
Cytoplasmic Foci 0.001206 0.012075 0.000051 0.000748 0.009872 0.002015 0.000053 0.000782 0.008454 0.006927 0.000053 0.000766
Eisosomes 0.000022 0.000081 0.001093 0.000085 0.000044 0.000097 0.000078 0.000083 0.000040 0.000089 0.000352 0.000084
Endoplasmic Reticulum 0.975010* 0.866291* 0.971065* 0.875111* 0.927108* 0.972359* 0.965980* 0.907952* 0.934943* 0.920573* 0.967352* 0.893155*
Endosome 0.003215 0.018192 0.005104 0.009246 0.007911 0.004446 0.003861 0.008408 0.007143 0.011158 0.004196 0.008786
Golgi 0.002204 0.009569 0.002113 0.003087 0.004678 0.002372 0.002247 0.003686 0.004274 0.005886 0.002211 0.003416
Lipid Particles 0.002128 0.012250 0.002606 0.024173 0.004133 0.003234 0.003096 0.015788 0.003805 0.007636 0.002964 0.019566
Mitochondria 0.000884 0.001574 0.000011 0.000350 0.001117 0.000355 0.000029 0.000484 0.001079 0.000950 0.000024 0.000424
Mitotic Spindle 0.000029 0.000372 0.000047 0.000669 0.000120 0.000566 0.000095 0.000662 0.000105 0.000472 0.000082 0.000666
None 0.000063 0.004425 0.000001 0.000821 0.002355 0.000479 0.000010 0.000628 0.001980 0.002405 0.000007 0.000715
Nuclear Periphery 0.000288 0.000678 0.000700 0.002400 0.000584 0.000185 0.000733 0.001128 0.000536 0.000426 0.000724 0.001702
Nuclear Periphery Foci 0.001150 0.013160 0.004554 0.015874 0.006285 0.001360 0.002467 0.010473 0.005445 0.007121 0.003030 0.012907
Nucleolus 0.000003 0.000097 0.000001 0.000024 0.000025 0.000005 0.000001 0.000013 0.000021 0.000050 0.000001 0.000018
Nucleus 0.000039 0.000567 0.000023 0.001074 0.000252 0.000074 0.000067 0.000536 0.000217 0.000315 0.000055 0.000779
Peroxisomes 0.000352 0.000650 0.000009 0.000035 0.000518 0.000264 0.000004 0.000095 0.000491 0.000453 0.000006 0.000068
Vacuole 0.005063 0.012209 0.003560 0.028944 0.007040 0.001904 0.005386 0.020096 0.006716 0.006935 0.004893 0.024082
Vacuole Periphery 0.000615 0.003385 0.000132 0.001016 0.002978 0.000672 0.000353 0.001393 0.002592 0.001997 0.000293 0.001223

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.73 -0.86 -1.78 0.86 0.17 -1.19 -0.00 -1.09 0.13 -0.90 -2.99 -0.71 -2.13 0.73 -0.68
Bud Neck -3.31 -1.13 -6.20 -5.08 -3.13 1.06 -0.69 -4.66 -5.09 -3.67 -2.64 -1.30 -7.42 -6.42 -5.26
Bud Site -7.26 0.82 -5.65 0.23 -5.85 2.24 3.70 0.05 -1.91 -3.19 -2.91 4.05 -3.08 -0.47 -6.21
Cell Periphery -0.91 -1.45 -7.31 -7.37 -4.59 -0.84 -2.90 -8.40 -7.64 -0.06 -1.79 -3.11 -11.87 -10.52 -0.88
Cytoplasm -10.69 2.56 -5.30 5.64 -7.96 9.58 9.94 3.71 -5.50 -6.22 -0.16 10.46 2.18 2.54 -9.34
Cytoplasmic Foci -8.08 1.96 0.50 9.14 -4.62 6.39 8.60 7.74 2.21 -4.23 1.33 8.74 7.78 9.18 -6.11
Eisosomes -5.04 -1.02 -5.45 -0.50 0.96 -1.76 -1.47 -3.18 0.41 -0.20 -2.94 -1.10 -5.00 0.17 0.94
Endoplasmic Reticulum 13.42 0.39 13.01 0.76 10.02 -8.86 -4.46 3.67 11.62 6.95 2.88 -4.59 9.14 6.75 11.58
Endosome -12.93 -0.97 -6.91 7.69 -2.45 4.61 2.74 -0.98 -5.33 -3.24 -5.72 2.39 -3.32 2.90 -4.23
Golgi -4.09 0.06 -0.55 4.96 -0.92 2.50 2.64 0.95 -1.31 -1.45 -1.94 2.59 1.00 3.09 -1.62
Lipid Particles -6.39 -0.28 -8.12 -4.47 -7.51 0.96 0.52 -6.82 -7.09 -6.01 -4.31 0.55 -10.30 -7.58 -9.32
Mitochondria -0.86 1.24 0.66 2.88 -7.30 3.72 5.60 2.72 -1.07 -3.93 0.44 5.30 2.93 2.33 -6.21
Mitotic Spindle -3.81 -0.54 -3.04 -1.89 -2.98 -1.22 -0.21 -2.36 -0.50 -2.10 -1.92 -0.16 -3.67 -1.47 -3.36
None -4.38 1.71 -1.91 3.36 -2.08 2.82 3.62 2.57 -0.69 -3.75 -0.57 3.64 2.18 3.16 -3.54
Nuclear Periphery -2.78 -2.09 -5.66 -4.72 -4.42 2.49 -0.84 -3.08 -9.11 -3.02 0.79 -1.23 -5.54 -7.20 -5.27
Nuclear Periphery Foci -8.50 -1.27 -8.28 -1.82 -3.95 4.95 2.93 -2.73 -6.23 -4.86 -1.60 1.92 -5.99 -4.89 -7.02
Nucleolus -1.71 2.74 -5.22 1.26 -5.75 5.16 6.35 2.41 -3.93 -6.00 -1.08 6.49 0.12 1.10 -7.85
Nucleus -7.41 1.65 -5.72 -3.38 -5.79 6.34 2.72 -2.64 -3.76 -3.49 -2.49 3.25 -5.64 -4.81 -6.48
Peroxisomes -1.35 1.94 1.79 4.66 -3.31 2.02 5.05 4.09 2.23 -4.68 0.32 5.40 4.67 5.10 -5.66
Vacuole -3.77 0.85 -9.25 -7.37 -10.73 6.28 1.02 -7.22 -10.30 -7.22 -0.25 1.48 -11.73 -11.84 -12.03
Vacuole Periphery -5.63 2.05 -1.92 4.92 -6.65 4.51 5.33 2.85 -2.55 -4.12 1.27 5.57 2.99 2.57 -6.16
Standard name
Human Ortholog
Description Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Pga3

Pga3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pga3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available