Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 4 | 5 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 4 | 2 | 0 | 2 | 2 | 1 | 1 | 5 | 1 | 7 | 13 | 11 |
Bud | 2 | 4 | 7 | 6 | 1 | 12 | 11 | – | 11 | 18 | 11 | 12 | 10 | 10 | 5 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Bud Site | 1 | 0 | 0 | 1 | 0 | 3 | 1 | – | 8 | 4 | 10 | 4 | 0 | 0 | 0 | 3 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 4 | 9 | 5 | 2 | 1 | 6 | – | 3 | 8 | 7 | 5 | 3 | 3 | 2 | 7 | 2 | 8 | 0 | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | 2 | 6 | 4 | 10 | 13 | 9 | 23 | – | 5 | 12 | 6 | 4 | 3 | 10 | 11 | 15 | 5 | 6 | 0 | 0 | 1 | 0 | 0 | 3 |
Endoplasmic Reticulum | 1 | 15 | 2 | 1 | 0 | 2 | 1 | – | 2 | 1 | 0 | 2 | 1 | 0 | 1 | 1 | 3 | 2 | 0 | 0 | 0 | 1 | 1 | 1 |
Endosome | 8 | 3 | 23 | 37 | 87 | 19 | 84 | – | 13 | 24 | 6 | 4 | 30 | 66 | 86 | 31 | 11 | 5 | 23 | 29 | 23 | 17 | 34 | 33 |
Golgi | 5 | 1 | 11 | 2 | 2 | 0 | 0 | – | 1 | 0 | 0 | 0 | 6 | 6 | 10 | 29 | 20 | 12 | 7 | 19 | 8 | 9 | 21 | 20 |
Mitochondria | 124 | 228 | 112 | 163 | 165 | 239 | 291 | – | 391 | 375 | 333 | 378 | 137 | 132 | 126 | 9 | 7 | 3 | 95 | 182 | 162 | 77 | 82 | 85 |
Nucleus | 0 | 0 | 2 | 0 | 5 | 7 | 17 | – | 6 | 8 | 4 | 4 | 0 | 0 | 1 | 2 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 7 | 1 | 0 | 0 | 3 | 3 | 11 | – | 20 | 22 | 12 | 16 | 1 | 0 | 4 | 6 | 2 | 6 | 2 | 2 | 2 | 1 | 1 | 1 |
Nucleolus | 2 | 1 | 0 | 2 | 2 | 3 | 5 | – | 12 | 16 | 11 | 15 | 2 | 3 | 3 | 5 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 0 |
Peroxisomes | 6 | 8 | 35 | 45 | 48 | 8 | 40 | – | 13 | 25 | 7 | 11 | 44 | 118 | 96 | 37 | 21 | 17 | 26 | 20 | 40 | 42 | 73 | 64 |
SpindlePole | 3 | 5 | 38 | 27 | 33 | 13 | 47 | – | 20 | 31 | 13 | 20 | 12 | 25 | 20 | 18 | 14 | 1 | 2 | 2 | 2 | 1 | 3 | 3 |
Vac/Vac Membrane | 6 | 16 | 11 | 7 | 42 | 13 | 40 | – | 22 | 22 | 10 | 13 | 9 | 5 | 11 | 6 | 4 | 6 | 0 | 7 | 0 | 1 | 1 | 2 |
Unique Cell Count | 135 | 252 | 189 | 230 | 301 | 271 | 424 | 421 | 452 | 356 | 414 | 180 | 246 | 262 | 131 | 78 | 56 | 163 | 275 | 248 | 168 | 241 | 234 | |
Labelled Cell Count | 168 | 295 | 258 | 311 | 403 | 333 | 577 | 527 | 567 | 431 | 488 | 262 | 380 | 376 | 174 | 97 | 70 | 163 | 275 | 248 | 168 | 241 | 234 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 4.9 | 6.3 | 5.7 | 6.8 | 4.7 | 6.0 | – | 5.3 | 5.5 | 5.3 | 5.4 | 6.0 | 6.4 | 6.4 | 7.7 | 7.9 | 8.6 | 5.5 | 5.9 | 5.9 |
Std Deviation (1e-4) | 1.0 | 0.7 | 1.7 | 1.1 | 2.0 | 1.4 | 1.5 | – | 1.4 | 1.4 | 1.2 | 1.1 | 1.1 | 1.7 | 1.6 | 1.7 | 1.8 | 2.2 | 1.6 | 1.8 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.14 | 0.11 | -0.43 | -0.08 | – | -0.24 | -0.18 | -0.25 | -0.22 | -0.07 | 0.04 | 0.02 | 0.29 | 0.33 | 0.45 | -0.18 | -0.09 | -0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | -0.5 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0074 | 0.772 | 0.6874 | 0.6768 | 0.4751 | 0.9017 | 2.2939 | 3.3857 | 3.1285 | 3.4054 | 3.1179 | 2.6809 | 4.9197 | 4.8912 | 4.7352 | 4.5272 | 4.5712 | 4.961 |
Actin | 0.0511 | 0.0102 | 0.0462 | 0.0183 | 0.0289 | 0.0191 | 0.0461 | 0.0215 | 0.0302 | 0.0098 | 0.0274 | 0.0241 | 0.0121 | 0.0894 | 0.1639 | 0.0008 | 0.0085 | 0.0875 |
Bud | 0.0008 | 0.0005 | 0.001 | 0.0002 | 0.0005 | 0.0004 | 0.0005 | 0.0005 | 0.0009 | 0.0019 | 0.0005 | 0.0002 | 0.0014 | 0.0011 | 0.0014 | 0.0001 | 0.0007 | 0.0003 |
Bud Neck | 0.0009 | 0.0006 | 0.002 | 0.0024 | 0.0002 | 0.0015 | 0.0004 | 0.0006 | 0.0006 | 0.0002 | 0.0004 | 0.0004 | 0.0007 | 0.0004 | 0.0009 | 0.0001 | 0.0002 | 0.0016 |
Bud Periphery | 0.0014 | 0.001 | 0.0013 | 0.0004 | 0.0013 | 0.0008 | 0.0009 | 0.001 | 0.0024 | 0.0041 | 0.002 | 0.0005 | 0.0013 | 0.0013 | 0.0029 | 0.0002 | 0.0018 | 0.0008 |
Bud Site | 0.0027 | 0.0092 | 0.0197 | 0.0004 | 0.0008 | 0.0009 | 0.0013 | 0.0042 | 0.0032 | 0.0007 | 0.0008 | 0.0004 | 0.0027 | 0.0042 | 0.0033 | 0.0002 | 0.0017 | 0.0007 |
Cell Periphery | 0.0004 | 0.0014 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0072 | 0.0017 | 0.0001 | 0.0009 | 0.0003 | 0.0001 | 0.0016 | 0.0016 | 0.0001 | 0.0002 | 0.0008 |
Cytoplasm | 0.0041 | 0.0101 | 0.0014 | 0.0003 | 0.0003 | 0.0004 | 0.0014 | 0.0145 | 0.0031 | 0.0012 | 0.001 | 0.0007 | 0.0237 | 0.0055 | 0.0025 | 0.0001 | 0.0002 | 0.0019 |
Cytoplasmic Foci | 0.0373 | 0.038 | 0.0413 | 0.0251 | 0.0139 | 0.0262 | 0.0285 | 0.0217 | 0.0201 | 0.0134 | 0.0263 | 0.0244 | 0.0475 | 0.0369 | 0.027 | 0.0155 | 0.0072 | 0.0249 |
Eisosomes | 0.0006 | 0.0008 | 0.0008 | 0.0002 | 0.0006 | 0.0003 | 0.0012 | 0.0027 | 0.0008 | 0.0001 | 0.0004 | 0.0006 | 0.0001 | 0.0025 | 0.0018 | 0.0001 | 0.0003 | 0.0019 |
Endoplasmic Reticulum | 0.0017 | 0.0028 | 0.0006 | 0.0001 | 0.0006 | 0.0002 | 0.0028 | 0.0007 | 0.0006 | 0.0004 | 0.001 | 0.0009 | 0.0014 | 0.0019 | 0.0011 | 0.0001 | 0.0002 | 0.0005 |
Endosome | 0.0709 | 0.0667 | 0.047 | 0.0302 | 0.0309 | 0.0399 | 0.0888 | 0.0543 | 0.0605 | 0.0859 | 0.0912 | 0.0373 | 0.0843 | 0.0506 | 0.0358 | 0.0397 | 0.0437 | 0.0326 |
Golgi | 0.1047 | 0.1293 | 0.0444 | 0.0251 | 0.0335 | 0.082 | 0.1138 | 0.1035 | 0.1003 | 0.0848 | 0.0956 | 0.1064 | 0.27 | 0.0657 | 0.0691 | 0.0675 | 0.0702 | 0.087 |
Lipid Particles | 0.0873 | 0.0449 | 0.0576 | 0.0253 | 0.1289 | 0.0555 | 0.0412 | 0.0264 | 0.0168 | 0.0258 | 0.013 | 0.017 | 0.0025 | 0.0018 | 0.0474 | 0.0042 | 0.0073 | 0.0188 |
Mitochondria | 0.4329 | 0.5434 | 0.5856 | 0.5646 | 0.6397 | 0.6256 | 0.5813 | 0.6303 | 0.6878 | 0.7354 | 0.6456 | 0.6902 | 0.0682 | 0.6651 | 0.5167 | 0.8375 | 0.8045 | 0.6632 |
None | 0.0025 | 0.0008 | 0.0077 | 0.0002 | 0.0012 | 0.0007 | 0.0137 | 0.0067 | 0.0012 | 0.0002 | 0.0002 | 0.0005 | 0.0084 | 0.0011 | 0.0013 | 0.0001 | 0.0002 | 0.0038 |
Nuclear Periphery | 0.0093 | 0.0009 | 0.0016 | 0.0004 | 0.0028 | 0.0001 | 0.0008 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0397 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0001 |
Nucleolus | 0.0018 | 0.0001 | 0.0015 | 0.0001 | 0.0003 | 0.0004 | 0.0005 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0054 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 |
Nucleus | 0.0099 | 0.0002 | 0.0007 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.3234 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 |
Peroxisomes | 0.1684 | 0.1344 | 0.1346 | 0.3049 | 0.1108 | 0.1413 | 0.0639 | 0.0939 | 0.0598 | 0.0236 | 0.0828 | 0.0919 | 0.014 | 0.0678 | 0.1088 | 0.0313 | 0.0497 | 0.0716 |
Punctate Nuclear | 0.0019 | 0.0002 | 0.0012 | 0.0005 | 0.0007 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0902 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 |
Vacuole | 0.0048 | 0.0019 | 0.0018 | 0.0005 | 0.0013 | 0.0017 | 0.0073 | 0.0062 | 0.0063 | 0.0066 | 0.0046 | 0.0013 | 0.0015 | 0.0011 | 0.0076 | 0.0008 | 0.001 | 0.0007 |
Vacuole Periphery | 0.0046 | 0.0026 | 0.0019 | 0.0006 | 0.002 | 0.0024 | 0.0045 | 0.0026 | 0.0029 | 0.0053 | 0.006 | 0.0022 | 0.0014 | 0.0013 | 0.0053 | 0.0016 | 0.002 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.1654 | 21.4789 | 8.5821 | 6.0608 | 13.1798 | 12.9306 | 16.7056 | 9.5839 | 8.2398 | 12.9706 |
Translational Efficiency | 1.2669 | 1.068 | 0.6235 | 0.6678 | 0.9177 | 0.898 | 1.3486 | 1.4187 | 1.1659 | 1.1577 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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