Standard name | |
---|---|
Human Ortholog | |
Description | C-22 sterol desaturase; a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 3 | 16 | 23 | 28 | 14 | 21 | 12 | 33 | 40 | 8 | 22 | 8 | 15 | 3 | 23 | 14 | 8 | 4 | 3 | 5 | 2 | 2 | 5 |
Bud | 0 | 0 | 2 | 3 | 5 | 2 | 8 | 4 | 5 | 11 | 2 | 3 | 1 | 0 | 1 | 2 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 12 | 21 | 9 | 15 | 13 | 18 | 10 | 27 | 27 | 9 | 19 | 19 | 55 | 43 | 34 | 26 | 23 | 1 | 0 | 1 | 1 | 3 | 3 |
Cytoplasm | 20 | 21 | 20 | 15 | 21 | 18 | 41 | 27 | 41 | 40 | 24 | 27 | 11 | 26 | 54 | 78 | 54 | 37 | 3 | 8 | 2 | 5 | 6 | 4 |
Endoplasmic Reticulum | 216 | 253 | 200 | 162 | 198 | 138 | 229 | 142 | 196 | 210 | 52 | 90 | 125 | 246 | 166 | 6 | 3 | 1 | 267 | 270 | 230 | 163 | 199 | 188 |
Endosome | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Golgi | 4 | 1 | 1 | 9 | 15 | 3 | 5 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 8 | 27 | 18 | 0 | 0 | 2 | 2 | 2 | 2 |
Mitochondria | 5 | 1 | 6 | 21 | 22 | 14 | 30 | 20 | 37 | 60 | 19 | 34 | 4 | 0 | 0 | 10 | 2 | 5 | 0 | 1 | 5 | 2 | 1 | 4 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 4 | 5 | 7 | 9 | 10 | 7 | 12 | 4 | 20 | 25 | 4 | 7 | 1 | 4 | 1 | 1 | 0 | 0 | 2 | 1 | 3 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 39 | 4 | 6 | 12 | 35 | 10 | 35 | 39 | 12 | 38 | 15 | 9 | 10 | 4 | 5 | 53 | 44 | 29 | 1 | 2 | 1 | 1 | 2 | 8 |
Unique Cell Count | 261 | 274 | 237 | 199 | 277 | 184 | 345 | 225 | 295 | 353 | 112 | 162 | 155 | 302 | 232 | 203 | 160 | 110 | 289 | 293 | 258 | 187 | 222 | 226 |
Labelled Cell Count | 300 | 301 | 279 | 265 | 350 | 219 | 402 | 261 | 371 | 455 | 135 | 213 | 181 | 350 | 273 | 237 | 180 | 128 | 289 | 293 | 258 | 187 | 222 | 226 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 22.1 | 24.0 | 14.1 | 14.9 | 16.3 | 14.5 | 12.1 | 12.2 | 12.1 | 11.8 | 10.9 | 11.2 | 16.5 | 15.5 | 10.7 | 12.2 | 15.1 | 16.5 | 19.1 | 20.7 | 21.6 |
Std Deviation (1e-4) | 4.9 | 5.2 | 2.9 | 3.9 | 4.0 | 3.0 | 3.0 | 2.9 | 2.7 | 2.7 | 2.9 | 2.5 | 6.1 | 3.1 | 2.2 | 3.1 | 3.6 | 4.0 | 5.3 | 4.7 | 4.9 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.21 | 0.04 | -0.22 | -0.21 | -0.22 | -0.26 | -0.38 | -0.33 | 0.23 | 0.13 | -0.4 | -0.21 | 0.09 | 0.23 | 0.43 | 0.55 | 0.61 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.8 | 1.4 | 0.3 | -0.3 | -0.6 | 1.8 | 1.9 | 0.1 | 2.3 | -0.6 | -0.9 | -3.0 | 1.7 | 0.7 | 0.2 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.8 | -1.5 | -0.7 | -1.7 | -1.9 | 0.1 | -0.5 | -0.3 | 0.9 | 1.1 | 3.1 | 3.1 | 2.5 | 2.2 | 3.1 |
Cytoplasm | -0.3 | -0.4 | 0.5 | 1.3 | 1.3 | 2.0 | 1.1 | 3.4 | 2.5 | -0.5 | 0.1 | 4.4 | 7.5 | 6.4 | 5.9 |
Endoplasmic Reticulum | -0.8 | -3.5 | -2.4 | -4.8 | -5.2 | -4.7 | -6.4 | -7.4 | -6.3 | -1.0 | -0.9 | -3.4 | -17.1 | -16.1 | -14.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.3 | 6.1 |
Mitochondria | 3.5 | 2.7 | 2.4 | 3.0 | 3.0 | 4.2 | 5.5 | 4.9 | 6.0 | 0 | 0 | 0 | 1.3 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.9 | 0.4 | 0.5 | 0.3 | -0.8 | 2.0 | 2.2 | 0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.8 | 4.2 | 1.5 | 3.5 | 5.4 | 1.0 | 3.7 | 4.0 | 1.6 | 1.9 | 0 | -0.3 | 7.2 | 7.4 | 6.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 20.6955 | 23.9802 | 21.4878 | 21.6558 | 22.1997 | 22.063 | 17.2831 | 21.655 | 19.7989 | 20.1821 | 19.4634 | 19.9841 | 5.68 | 21.4743 | 18.7623 | 19.877 | 21.6775 | 20.558 |
Actin | 0.011 | 0.0027 | 0.0122 | 0.0002 | 0.0001 | 0.0015 | 0.0612 | 0.0003 | 0.0064 | 0.0008 | 0.0253 | 0.0005 | 0.2709 | 0.0001 | 0.0075 | 0.0198 | 0 | 0.0002 |
Bud | 0.0004 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.001 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0008 | 0 | 0.0001 | 0.0006 | 0 | 0 |
Bud Neck | 0.0008 | 0.0006 | 0.0005 | 0.0005 | 0.0004 | 0.0008 | 0.0016 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0006 | 0.0008 | 0 | 0.0006 | 0.0004 | 0.0001 | 0.0002 |
Bud Periphery | 0.0008 | 0.0002 | 0.0005 | 0 | 0 | 0.0002 | 0.001 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0 | 0.0014 | 0 | 0.0002 | 0.0016 | 0 | 0 |
Bud Site | 0.0022 | 0.0013 | 0.001 | 0 | 0 | 0 | 0.0086 | 0.0002 | 0.0002 | 0 | 0.0007 | 0 | 0.0017 | 0 | 0.0005 | 0.0002 | 0 | 0 |
Cell Periphery | 0.0011 | 0.0064 | 0.0084 | 0.0004 | 0.0015 | 0.0036 | 0.0023 | 0.01 | 0.0016 | 0.0016 | 0.0004 | 0.0005 | 0.0007 | 0.0003 | 0.0013 | 0.0035 | 0.0002 | 0.0003 |
Cytoplasm | 0.0149 | 0.049 | 0.0196 | 0.0117 | 0.0021 | 0.0052 | 0.0277 | 0.0374 | 0.0246 | 0.0017 | 0.008 | 0.0034 | 0.0115 | 0.0019 | 0.0143 | 0.0068 | 0.0006 | 0.0018 |
Cytoplasmic Foci | 0.0139 | 0.0009 | 0.0054 | 0.0001 | 0.0001 | 0.0007 | 0.0044 | 0.0004 | 0.0024 | 0.0001 | 0.0122 | 0.0012 | 0.007 | 0 | 0.0049 | 0.0004 | 0 | 0 |
Eisosomes | 0.0004 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0006 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0025 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.7467 | 0.855 | 0.861 | 0.9706 | 0.9861 | 0.9364 | 0.7286 | 0.9167 | 0.9116 | 0.9708 | 0.8473 | 0.96 | 0.497 | 0.97 | 0.8961 | 0.9098 | 0.9881 | 0.9739 |
Endosome | 0.0112 | 0.0027 | 0.0111 | 0.0007 | 0.0004 | 0.0025 | 0.028 | 0.0012 | 0.0094 | 0.0033 | 0.0294 | 0.009 | 0.0717 | 0.0003 | 0.0181 | 0.018 | 0.0004 | 0.0007 |
Golgi | 0.0027 | 0.0011 | 0.0037 | 0.0002 | 0.0001 | 0.0037 | 0.0059 | 0.0003 | 0.0058 | 0.0015 | 0.0284 | 0.0044 | 0.024 | 0 | 0.002 | 0.0147 | 0.0001 | 0.0001 |
Lipid Particles | 0.0121 | 0.0098 | 0.0123 | 0.0005 | 0.0006 | 0.0016 | 0.0053 | 0.0065 | 0.006 | 0.0011 | 0.0038 | 0.0019 | 0.0325 | 0.0001 | 0.0038 | 0.0008 | 0.0001 | 0.0001 |
Mitochondria | 0.0091 | 0.0005 | 0.0196 | 0 | 0 | 0.0023 | 0.0083 | 0.0007 | 0.0007 | 0.002 | 0.0169 | 0.0005 | 0.0064 | 0.0001 | 0.0008 | 0.0083 | 0 | 0 |
None | 0.0249 | 0.0001 | 0.0019 | 0 | 0 | 0 | 0.0086 | 0.0024 | 0.0014 | 0.0004 | 0.0002 | 0.0001 | 0.0018 | 0 | 0.004 | 0.0002 | 0 | 0 |
Nuclear Periphery | 0.0982 | 0.0523 | 0.0274 | 0.0124 | 0.006 | 0.026 | 0.0802 | 0.0114 | 0.0246 | 0.0109 | 0.0205 | 0.0144 | 0.0449 | 0.0246 | 0.0306 | 0.0102 | 0.0083 | 0.0206 |
Nucleolus | 0.0033 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0009 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 |
Nucleus | 0.0128 | 0.0065 | 0.0013 | 0.0007 | 0.0002 | 0.0015 | 0.0114 | 0.0004 | 0.0013 | 0.0004 | 0.0005 | 0.0004 | 0.0012 | 0.0006 | 0.003 | 0.0005 | 0.0002 | 0.0006 |
Peroxisomes | 0.0075 | 0 | 0.0008 | 0 | 0 | 0.0003 | 0.0012 | 0 | 0.0004 | 0 | 0.0017 | 0 | 0.0011 | 0 | 0.0008 | 0 | 0 | 0 |
Punctate Nuclear | 0.0134 | 0.0002 | 0.0012 | 0.0001 | 0 | 0.0001 | 0.0037 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0009 | 0 | 0.007 | 0.0001 | 0 | 0 |
Vacuole | 0.0078 | 0.0086 | 0.0092 | 0.0017 | 0.0022 | 0.0129 | 0.0059 | 0.0105 | 0.002 | 0.0025 | 0.0018 | 0.0025 | 0.0189 | 0.0012 | 0.0028 | 0.0023 | 0.0014 | 0.0009 |
Vacuole Periphery | 0.0048 | 0.0018 | 0.0023 | 0.0002 | 0.0002 | 0.0007 | 0.0036 | 0.0011 | 0.0011 | 0.0015 | 0.0016 | 0.0005 | 0.0023 | 0.0007 | 0.0011 | 0.0016 | 0.0003 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 56.9785 | 68.9304 | 88.7192 | 97.9357 | 64.5855 | 36.828 | 78.0219 | 81.3829 | 89.0911 | 81.8082 |
Translational Efficiency | 1.3728 | 1.641 | 1.5258 | 1.3679 | 1.5085 | 1.7026 | 1.3169 | 1.3584 | 1.5064 | 1.394 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1973 | 924 | 1498 | 1398 | 733 | 1262 | 443 | 92 | 2706 | 2186 | 1941 | 1490 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1535.88 | 1511.42 | 2569.54 | 1801.68 | 1484.46 | 1701.38 | 1930.98 | 1949.89 | 1521.95 | 1621.09 | 2423.80 | 1810.83 |
Standard Deviation | 280.74 | 384.88 | 759.42 | 402.31 | 259.57 | 338.81 | 320.61 | 522.17 | 276.11 | 371.07 | 735.11 | 412.27 |
Intensity Change Log 2 | — | -0.023161 | 0.742445 | 0.230277 | — | 0.196767 | 0.379395 | 0.393455 | — | 0.089120 | 0.575381 | 0.312784 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000268 | 0.000993 | 0.001377 | 0.002936 | 0.001189 | 0.001336 | 0.004125 | 0.005667 | 0.000517 | 0.001191 | 0.002004 | 0.003104 |
Bud Neck | 0.000191 | 0.001009 | 0.008669 | 0.016489 | 0.000155 | 0.000308 | 0.003469 | 0.022253 | 0.000181 | 0.000604 | 0.007482 | 0.016845 |
Bud Site | 0.002431 | 0.010395 | 0.012430 | 0.035124 | 0.001281 | 0.005157 | 0.005613 | 0.036066 | 0.002119 | 0.007371 | 0.010874 | 0.035183 |
Cell Periphery | 0.004549 | 0.006463 | 0.012739 | 0.019380 | 0.001759 | 0.002116 | 0.007617 | 0.012201 | 0.003793 | 0.003954 | 0.011570 | 0.018937 |
Cytoplasm | 0.003771 | 0.018817 | 0.031359 | 0.019588 | 0.001233 | 0.004862 | 0.006821 | 0.009681 | 0.003083 | 0.010761 | 0.025758 | 0.018976 |
Cytoplasmic Foci | 0.000652 | 0.013501 | 0.002944 | 0.010581 | 0.000157 | 0.002329 | 0.001555 | 0.024537 | 0.000518 | 0.007052 | 0.002627 | 0.011443 |
Eisosomes | 0.000101 | 0.000067 | 0.000236 | 0.000182 | 0.000027 | 0.000055 | 0.000192 | 0.000241 | 0.000081 | 0.000060 | 0.000226 | 0.000186 |
Endoplasmic Reticulum | 0.975892* | 0.809088* | 0.747500* | 0.644699* | 0.983627* | 0.954391* | 0.891176* | 0.467846* | 0.977987* | 0.892973* | 0.780291* | 0.633779* |
Endosome | 0.002826 | 0.026084 | 0.018565 | 0.026078 | 0.002961 | 0.007550 | 0.009743 | 0.041137 | 0.002862 | 0.015384 | 0.016551 | 0.027008 |
Golgi | 0.000734 | 0.021773 | 0.002475 | 0.006688 | 0.000433 | 0.003396 | 0.001251 | 0.010989 | 0.000653 | 0.011164 | 0.002196 | 0.006954 |
Lipid Particles | 0.003078 | 0.016380 | 0.019583 | 0.141791* | 0.000780 | 0.002441 | 0.021643 | 0.115677* | 0.002455 | 0.008333 | 0.020053 | 0.140179* |
Mitochondria | 0.000569 | 0.001577 | 0.001186 | 0.000773 | 0.000147 | 0.000556 | 0.000160 | 0.002313 | 0.000454 | 0.000988 | 0.000952 | 0.000868 |
Mitotic Spindle | 0.000047 | 0.000412 | 0.003674 | 0.005958 | 0.000019 | 0.000554 | 0.001734 | 0.054819 | 0.000039 | 0.000494 | 0.003231 | 0.008975 |
None | 0.001597 | 0.006193 | 0.002570 | 0.001874 | 0.000195 | 0.000843 | 0.000041 | 0.000055 | 0.001218 | 0.003105 | 0.001993 | 0.001761 |
Nuclear Periphery | 0.000329 | 0.002690 | 0.031626 | 0.002599 | 0.001086 | 0.000837 | 0.003858 | 0.009317 | 0.000534 | 0.001620 | 0.025289 | 0.003014 |
Nuclear Periphery Foci | 0.001363 | 0.029854 | 0.072448 | 0.043624 | 0.002058 | 0.005014 | 0.027704 | 0.124326 | 0.001552 | 0.015514 | 0.062236 | 0.048607 |
Nucleolus | 0.000007 | 0.000218 | 0.000040 | 0.000033 | 0.000002 | 0.000143 | 0.000007 | 0.000127 | 0.000006 | 0.000175 | 0.000032 | 0.000039 |
Nucleus | 0.000203 | 0.001194 | 0.006037 | 0.001825 | 0.000072 | 0.000497 | 0.000933 | 0.002081 | 0.000167 | 0.000792 | 0.004872 | 0.001841 |
Peroxisomes | 0.000258 | 0.000221 | 0.000030 | 0.000288 | 0.000044 | 0.000173 | 0.000029 | 0.002294 | 0.000200 | 0.000193 | 0.000030 | 0.000412 |
Vacuole | 0.000878 | 0.023573 | 0.023323 | 0.018192 | 0.002422 | 0.005829 | 0.012141 | 0.054929 | 0.001296 | 0.013330 | 0.020771 | 0.020461 |
Vacuole Periphery | 0.000258 | 0.009499 | 0.001189 | 0.001296 | 0.000352 | 0.001609 | 0.000188 | 0.003443 | 0.000283 | 0.004944 | 0.000961 | 0.001428 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.62 | -4.97 | -6.31 | -4.81 | -3.56 | -0.16 | -1.56 | -1.90 | -1.95 | -0.23 | -1.82 | -3.10 | -5.73 | -4.54 | -2.03 |
Bud Neck | -5.51 | -10.95 | -13.76 | -13.10 | -5.07 | -2.24 | -3.67 | -4.70 | -4.67 | -3.97 | -5.28 | -11.52 | -14.52 | -14.18 | -6.79 |
Bud Site | -4.72 | -6.18 | -12.06 | -8.82 | -7.44 | -3.01 | -3.13 | -4.16 | -3.74 | -3.64 | -4.81 | -6.81 | -12.84 | -10.75 | -8.68 |
Cell Periphery | -1.99 | -7.19 | -10.25 | -8.15 | -3.08 | -0.64 | -3.14 | -2.29 | -2.21 | -0.82 | -0.28 | -7.99 | -11.16 | -10.96 | -3.91 |
Cytoplasm | -7.16 | -11.78 | -8.37 | -0.95 | 4.35 | -4.24 | -3.76 | -2.25 | -1.67 | -1.13 | -7.15 | -12.27 | -9.04 | -4.75 | 2.98 |
Cytoplasmic Foci | -7.14 | -4.06 | -7.05 | 0.73 | -5.04 | -4.16 | -1.96 | -3.97 | -3.66 | -3.74 | -7.88 | -4.55 | -7.98 | -3.30 | -6.12 |
Eisosomes | 0.79 | -1.59 | -1.95 | -4.67 | 0.65 | -1.15 | -2.68 | -2.16 | -1.82 | -0.16 | 0.65 | -2.21 | -3.12 | -5.51 | 0.65 |
Endoplasmic Reticulum | 16.86 | 27.60 | 33.63 | 12.99 | 7.13 | 6.36 | 8.80 | 12.91 | 12.19 | 10.25 | 16.30 | 28.46 | 35.93 | 24.93 | 11.63 |
Endosome | -14.85 | -17.51 | -20.55 | -0.11 | -4.27 | -6.42 | -6.32 | -6.11 | -5.31 | -4.86 | -15.56 | -18.51 | -21.46 | -8.57 | -6.88 |
Golgi | -8.35 | -6.78 | -10.08 | 5.85 | -6.56 | -3.88 | -3.43 | -3.17 | -2.08 | -2.88 | -8.98 | -7.56 | -10.76 | 3.34 | -7.62 |
Lipid Particles | -6.42 | -8.56 | -21.67 | -18.87 | -17.83 | -3.21 | -5.26 | -5.68 | -5.59 | -4.46 | -5.80 | -10.12 | -22.49 | -21.38 | -18.44 |
Mitochondria | -2.04 | -4.75 | -1.95 | 1.43 | 3.46 | -3.71 | -0.87 | -1.50 | -1.17 | -1.46 | -2.33 | -5.02 | -3.29 | 0.17 | 1.47 |
Mitotic Spindle | -3.54 | -4.85 | -5.57 | -5.28 | -2.00 | -1.84 | -2.16 | -4.02 | -4.00 | -3.92 | -2.62 | -5.28 | -6.53 | -6.26 | -4.25 |
None | -2.80 | -1.51 | -0.45 | 2.50 | 0.87 | -2.10 | 1.21 | 0.91 | 2.60 | -0.99 | -2.50 | -1.59 | -0.91 | 1.42 | 0.36 |
Nuclear Periphery | -3.81 | -15.57 | -11.71 | 0.01 | 14.34 | 0.65 | -3.89 | -2.15 | -2.23 | -1.29 | -3.80 | -15.66 | -8.33 | -3.63 | 13.88 |
Nuclear Periphery Foci | -8.59 | -18.45 | -16.40 | -3.52 | 6.36 | -3.53 | -6.05 | -4.88 | -4.75 | -3.67 | -9.30 | -19.23 | -16.41 | -10.32 | 3.54 |
Nucleolus | -1.55 | -5.77 | -6.98 | 1.31 | 1.03 | -1.18 | -1.57 | -2.43 | -0.11 | -2.33 | -1.87 | -5.98 | -6.94 | 1.41 | -0.99 |
Nucleus | -1.45 | -9.03 | -5.63 | -1.64 | 5.95 | -1.89 | -4.43 | -1.93 | -1.74 | -1.47 | -1.99 | -9.32 | -6.02 | -3.54 | 5.05 |
Peroxisomes | 0.42 | 3.07 | -0.43 | -0.99 | -4.48 | -1.73 | 0.61 | -2.04 | -1.92 | -2.05 | 0.04 | 3.11 | -2.16 | -2.22 | -4.34 |
Vacuole | -10.28 | -16.98 | -14.35 | 1.47 | 3.17 | -2.79 | -4.47 | -4.29 | -4.03 | -3.39 | -10.53 | -16.89 | -13.65 | -4.75 | 0.46 |
Vacuole Periphery | -7.10 | -6.66 | -6.84 | 6.26 | -0.41 | -3.84 | 1.35 | -1.93 | -0.98 | -2.04 | -7.96 | -6.14 | -6.79 | 5.86 | -2.24 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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