Standard name | |
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Human Ortholog | |
Description | Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; relocalizes to the cytosol in response to hypoxia; SOK2 has a paralog, PHD1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 2 | 4 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 1 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 2 | 3 | 7 | 3 | 6 | 7 | 11 | 13 | 2 | 0 | 1 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 2 | 4 | 3 | 5 | 10 | 5 | 12 | 5 | 6 | 3 | 4 | 10 | 33 | 33 | 44 | 28 | 29 | 1 | 0 | 1 | 1 | 0 | 1 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 5 | 3 | 0 | 0 | 0 | 1 | 1 | 4 |
Endosome | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 5 | 5 | 7 | 2 | 0 | 2 | 0 | 0 | 6 | 5 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 2 |
Mitochondria | 3 | 8 | 0 | 3 | 0 | 20 | 41 | 18 | 40 | 48 | 68 | 28 | 4 | 0 | 1 | 5 | 2 | 5 | 0 | 2 | 0 | 1 | 2 | 3 |
Nucleus | 117 | 334 | 174 | 97 | 180 | 211 | 255 | 255 | 138 | 157 | 167 | 187 | 183 | 217 | 177 | 300 | 269 | 246 | 120 | 343 | 164 | 64 | 136 | 151 |
Nuclear Periphery | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 2 | 1 | 4 | 2 | 1 | 0 | 3 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 44 | 83 | 21 | 18 | 14 | 53 | 62 | 52 | 79 | 98 | 103 | 87 | 54 | 60 | 51 | 39 | 27 | 23 | 10 | 36 | 11 | 30 | 57 | 50 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 5 | 1 | 0 | 2 | 1 | 3 | 1 | 0 | 0 | 3 | 5 | 2 | 3 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 3 | 4 |
Vac/Vac Membrane | 0 | 6 | 2 | 0 | 1 | 1 | 1 | 9 | 1 | 3 | 1 | 3 | 2 | 4 | 2 | 13 | 6 | 4 | 2 | 8 | 4 | 10 | 25 | 28 |
Unique Cell Count | 130 | 376 | 183 | 111 | 197 | 250 | 298 | 311 | 195 | 233 | 249 | 253 | 217 | 285 | 242 | 384 | 332 | 293 | 138 | 402 | 186 | 118 | 242 | 268 |
Labelled Cell Count | 168 | 437 | 207 | 125 | 205 | 303 | 374 | 355 | 273 | 327 | 360 | 330 | 265 | 326 | 278 | 416 | 348 | 316 | 138 | 402 | 186 | 118 | 242 | 268 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.0 | 9.8 | 8.2 | 7.3 | 8.2 | 6.7 | 6.3 | 7.1 | 6.0 | 5.8 | 5.7 | 6.1 | 9.1 | 8.7 | 8.7 | 10.5 | 11.4 | 10.6 | 8.2 | 7.9 | 8.0 |
Std Deviation (1e-4) | 2.0 | 2.0 | 1.3 | 1.3 | 1.3 | 1.5 | 1.2 | 1.8 | 1.4 | 1.4 | 1.2 | 1.3 | 2.4 | 2.6 | 2.6 | 2.2 | 2.2 | 2.3 | 2.1 | 1.6 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.0 | -0.31 | -0.38 | -0.22 | -0.46 | -0.51 | -0.54 | -0.43 | 0.14 | 0.07 | 0.08 | 0.35 | 0.47 | 0.36 | -0.01 | -0.06 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 1.1 | 0 | 1.0 | 0 | 0 | 0 | 0 | 1.3 | 3.7 | 4.1 | 3.7 | 2.8 | 3.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.9 | 5.2 | 3.3 | 6.5 | 6.5 | 7.7 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -2.4 | -1.4 | -3.5 | -3.3 | -4.2 | -6.2 | -7.0 | -7.1 | -5.8 | -3.5 | -5.4 | -5.9 | -5.1 | -4.4 | -3.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.2 | -1.5 | 2.7 | 2.6 | 1.6 | 6.4 | 6.9 | 6.8 | 5.5 | 3.4 | 2.7 | 2.6 | -0.5 | -1.2 | -1.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.1571 | 3.927 | 3.4455 | 3.2046 | 3.1001 | 3.7732 | 6.4517 | 6.5098 | 6.1855 | 5.5789 | 5.1576 | 6.2155 | 5.3825 | 5.5136 | 5.0643 | 5.2747 | 4.7086 | 5.1244 |
Actin | 0.0132 | 0 | 0.0103 | 0.0012 | 0.0088 | 0.0089 | 0.0279 | 0.0003 | 0.0089 | 0.0253 | 0.0017 | 0.0003 | 0.0392 | 0 | 0.0007 | 0.0026 | 0.0295 | 0.0002 |
Bud | 0.0007 | 0.0006 | 0.0002 | 0.0004 | 0.0004 | 0.0001 | 0.0011 | 0.0001 | 0.0003 | 0.0006 | 0.0035 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0023 | 0 |
Bud Neck | 0.0014 | 0.0001 | 0.0004 | 0.0189 | 0.003 | 0.0018 | 0.0028 | 0.0003 | 0.0017 | 0.0026 | 0.0034 | 0.0004 | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0159 | 0.0007 |
Bud Periphery | 0.0016 | 0.0006 | 0.0003 | 0.0006 | 0.0009 | 0.0003 | 0.0026 | 0.0001 | 0.0008 | 0.0006 | 0.0072 | 0 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0032 | 0.0001 |
Bud Site | 0.0026 | 0.0011 | 0.0022 | 0.0024 | 0.0014 | 0.0002 | 0.0062 | 0.007 | 0.0015 | 0.0027 | 0.0629 | 0.0001 | 0.0057 | 0.0002 | 0.0006 | 0.0001 | 0.0081 | 0.0001 |
Cell Periphery | 0.0006 | 0.0001 | 0.0003 | 0.0005 | 0.0004 | 0.0001 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0017 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0006 | 0 |
Cytoplasm | 0.0052 | 0.0003 | 0.0046 | 0.0003 | 0.0011 | 0.0002 | 0.006 | 0.0001 | 0.0006 | 0.0006 | 0.0004 | 0.0007 | 0.0098 | 0 | 0.0024 | 0.0001 | 0.0034 | 0.0001 |
Cytoplasmic Foci | 0.0346 | 0.0001 | 0.0199 | 0.0079 | 0.0731 | 0.0006 | 0.0237 | 0.0001 | 0.0048 | 0.0137 | 0.0006 | 0.0023 | 0.0583 | 0 | 0.0095 | 0.0079 | 0.0259 | 0.0027 |
Eisosomes | 0.0013 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0046 | 0.0001 | 0.0028 | 0.0001 | 0.0015 | 0.0012 | 0.0048 | 0 | 0.0006 | 0.0003 | 0.0002 | 0.0041 | 0.0152 | 0 | 0.0003 | 0 | 0.0012 | 0 |
Endosome | 0.0363 | 0.0001 | 0.0174 | 0.0111 | 0.0388 | 0.0017 | 0.0302 | 0 | 0.0037 | 0.0247 | 0.0011 | 0.0026 | 0.0226 | 0 | 0.0131 | 0.0051 | 0.0094 | 0.0018 |
Golgi | 0.0081 | 0 | 0.0035 | 0.0096 | 0.0215 | 0.002 | 0.0101 | 0 | 0.0026 | 0.0396 | 0.0007 | 0.0005 | 0.0084 | 0 | 0.0107 | 0.0129 | 0.0032 | 0.0007 |
Lipid Particles | 0.0231 | 0 | 0.024 | 0.0143 | 0.0527 | 0.0008 | 0.0265 | 0 | 0.0023 | 0.0119 | 0.0006 | 0.001 | 0.0177 | 0.0001 | 0.0065 | 0.0018 | 0.012 | 0.0045 |
Mitochondria | 0.0261 | 0.0008 | 0.02 | 0.005 | 0.0325 | 0.0012 | 0.0113 | 0.0011 | 0.0013 | 0.0178 | 0.0031 | 0.0095 | 0.0077 | 0.0001 | 0.0025 | 0.0032 | 0.0137 | 0.0001 |
None | 0.0124 | 0.0002 | 0.004 | 0.0007 | 0.0016 | 0.0001 | 0.0075 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0013 | 0.0043 | 0 | 0.0002 | 0 | 0.0011 | 0 |
Nuclear Periphery | 0.0246 | 0.0007 | 0.0114 | 0.0017 | 0.0047 | 0.018 | 0.0196 | 0.0006 | 0.003 | 0.0019 | 0.0017 | 0.0041 | 0.0174 | 0.0007 | 0.0007 | 0.0004 | 0.0109 | 0.0001 |
Nucleolus | 0.0545 | 0.0306 | 0.0363 | 0.0497 | 0.2289 | 0.1198 | 0.0361 | 0.0278 | 0.0254 | 0.0273 | 0.3234 | 0.0774 | 0.0277 | 0.0269 | 0.021 | 0.0142 | 0.1714 | 0.1195 |
Nucleus | 0.6717 | 0.9628 | 0.8269 | 0.8584 | 0.4422 | 0.8397 | 0.7352 | 0.96 | 0.9358 | 0.7945 | 0.5799 | 0.889 | 0.7294 | 0.9705 | 0.9223 | 0.9436 | 0.6241 | 0.867 |
Peroxisomes | 0.0222 | 0 | 0.0042 | 0.0051 | 0.0681 | 0.0009 | 0.0095 | 0.0001 | 0.0022 | 0.0187 | 0.0004 | 0.0003 | 0.0193 | 0 | 0.002 | 0.0075 | 0.0036 | 0.0014 |
Punctate Nuclear | 0.0413 | 0.0005 | 0.0069 | 0.0064 | 0.0065 | 0.0008 | 0.0294 | 0.0015 | 0.0005 | 0.0149 | 0.0031 | 0.006 | 0.0118 | 0.001 | 0.0065 | 0.0002 | 0.0356 | 0.0009 |
Vacuole | 0.0085 | 0.0009 | 0.0026 | 0.0041 | 0.0067 | 0.0007 | 0.0062 | 0.0001 | 0.0023 | 0.0011 | 0.0034 | 0.0003 | 0.0016 | 0 | 0.0007 | 0 | 0.0091 | 0.0001 |
Vacuole Periphery | 0.0053 | 0.0003 | 0.0015 | 0.0017 | 0.0052 | 0.0009 | 0.0022 | 0.0001 | 0.001 | 0.0006 | 0.0004 | 0.0003 | 0.0012 | 0 | 0.0002 | 0.0001 | 0.0157 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.7592 | 13.8042 | 13.9682 | 20.831 | 13.6509 | 18.2396 | 25.8002 | 26.4055 | 19.8184 | 22.8629 |
Translational Efficiency | 1.5679 | 1.9379 | 1.5372 | 0.7387 | 1.3912 | 2.2593 | 0.8407 | 1.0033 | 1.2894 | 1.256 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1621 | 1436 | 224 | 1341 | 2164 | 1796 | 205 | 126 | 3785 | 3232 | 429 | 1467 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 819.27 | 979.63 | 1136.99 | 1118.79 | 914.73 | 903.74 | 1114.46 | 1149.50 | 873.85 | 937.46 | 1126.22 | 1121.43 |
Standard Deviation | 122.20 | 179.76 | 207.95 | 160.75 | 144.24 | 181.07 | 173.55 | 157.75 | 143.25 | 184.38 | 192.61 | 160.73 |
Intensity Change Log 2 | — | 0.257898 | 0.472809 | 0.449528 | — | -0.017438 | 0.284927 | 0.329589 | — | 0.119213 | 0.376751 | 0.387501 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000391 | 0.000642 | 0.001298 | 0.001288 | 0.000377 | 0.000549 | 0.001164 | 0.001045 | 0.000383 | 0.000590 | 0.001234 | 0.001267 |
Bud Neck | 0.005243 | 0.008438 | 0.010931 | 0.037112 | 0.006906 | 0.008746 | 0.006602 | 0.010998 | 0.006194 | 0.008610 | 0.008862 | 0.034869 |
Bud Site | 0.001085 | 0.001100 | 0.001147 | 0.007645 | 0.000679 | 0.001377 | 0.000915 | 0.002040 | 0.000853 | 0.001254 | 0.001036 | 0.007163 |
Cell Periphery | 0.000061 | 0.000057 | 0.000041 | 0.000151 | 0.000044 | 0.000157 | 0.000061 | 0.000071 | 0.000051 | 0.000113 | 0.000051 | 0.000145 |
Cytoplasm | 0.002257 | 0.000523 | 0.000804 | 0.005193 | 0.000143 | 0.001431 | 0.003173 | 0.002286 | 0.001049 | 0.001028 | 0.001936 | 0.004944 |
Cytoplasmic Foci | 0.000363 | 0.000080 | 0.000073 | 0.002095 | 0.000125 | 0.000254 | 0.000135 | 0.000059 | 0.000227 | 0.000176 | 0.000103 | 0.001920 |
Eisosomes | 0.000033 | 0.000045 | 0.000067 | 0.000038 | 0.000048 | 0.000047 | 0.000058 | 0.000031 | 0.000042 | 0.000046 | 0.000063 | 0.000037 |
Endoplasmic Reticulum | 0.001583 | 0.001714 | 0.002552 | 0.002869 | 0.001721 | 0.002571 | 0.003560 | 0.001521 | 0.001662 | 0.002191 | 0.003033 | 0.002753 |
Endosome | 0.000575 | 0.000206 | 0.000072 | 0.002918 | 0.000342 | 0.000744 | 0.000127 | 0.000124 | 0.000442 | 0.000505 | 0.000098 | 0.002678 |
Golgi | 0.000272 | 0.000133 | 0.000137 | 0.003435 | 0.000098 | 0.000228 | 0.000108 | 0.000113 | 0.000172 | 0.000186 | 0.000123 | 0.003149 |
Lipid Particles | 0.000292 | 0.000030 | 0.000070 | 0.000213 | 0.000028 | 0.000242 | 0.000200 | 0.000128 | 0.000141 | 0.000148 | 0.000132 | 0.000206 |
Mitochondria | 0.000555 | 0.001099 | 0.001864 | 0.005632 | 0.000788 | 0.001474 | 0.001143 | 0.001332 | 0.000688 | 0.001307 | 0.001520 | 0.005263 |
Mitotic Spindle | 0.000926 | 0.001161 | 0.001738 | 0.014352 | 0.000173 | 0.003119 | 0.000350 | 0.002813 | 0.000496 | 0.002249 | 0.001075 | 0.013361 |
None | 0.004086 | 0.001294 | 0.001004 | 0.004356 | 0.001029 | 0.006402 | 0.008847 | 0.004153 | 0.002338 | 0.004132 | 0.004752 | 0.004339 |
Nuclear Periphery | 0.000097 | 0.000079 | 0.000164 | 0.001961 | 0.000066 | 0.000373 | 0.000190 | 0.000187 | 0.000079 | 0.000242 | 0.000177 | 0.001809 |
Nuclear Periphery Foci | 0.000265 | 0.000076 | 0.000086 | 0.001319 | 0.000113 | 0.000627 | 0.000133 | 0.000265 | 0.000178 | 0.000382 | 0.000108 | 0.001228 |
Nucleolus | 0.072686 | 0.068962 | 0.051935 | 0.020935 | 0.073473 | 0.085180 | 0.037487 | 0.025702 | 0.073136 | 0.077974 | 0.045031 | 0.021344 |
Nucleus | 0.907975* | 0.912277* | 0.921939* | 0.878784* | 0.912785* | 0.884377* | 0.932696* | 0.945208* | 0.910725* | 0.896773* | 0.927079* | 0.884489* |
Peroxisomes | 0.000518 | 0.000666 | 0.002864 | 0.000526 | 0.000420 | 0.000611 | 0.001090 | 0.000839 | 0.000462 | 0.000635 | 0.002016 | 0.000553 |
Vacuole | 0.000496 | 0.000752 | 0.001026 | 0.005391 | 0.000414 | 0.000954 | 0.001843 | 0.000959 | 0.000450 | 0.000864 | 0.001416 | 0.005011 |
Vacuole Periphery | 0.000239 | 0.000667 | 0.000188 | 0.003787 | 0.000227 | 0.000536 | 0.000119 | 0.000126 | 0.000232 | 0.000594 | 0.000155 | 0.003473 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.27 | -11.35 | -12.03 | -7.89 | -1.75 | -3.54 | -9.32 | -2.97 | -2.68 | -0.41 | -4.72 | -13.66 | -11.91 | -9.77 | -0.38 |
Bud Neck | -5.47 | -6.08 | -20.88 | -18.81 | -15.77 | -2.71 | -3.90 | -5.59 | -4.71 | -3.50 | -5.35 | -6.90 | -21.05 | -19.26 | -16.76 |
Bud Site | -0.13 | -0.68 | -5.19 | -5.17 | -5.14 | -2.46 | -4.29 | -2.26 | -1.93 | -1.60 | -1.78 | -3.79 | -5.83 | -5.40 | -5.02 |
Cell Periphery | 0.24 | 1.65 | -4.43 | -3.88 | -5.85 | -2.23 | -2.13 | -3.15 | 1.04 | -1.63 | -2.11 | -0.82 | -5.49 | -0.65 | -5.19 |
Cytoplasm | 2.41 | 1.93 | -1.83 | -3.70 | -3.37 | -4.16 | -2.21 | -1.93 | -0.69 | 0.10 | 0.15 | -0.71 | -3.22 | -3.36 | -2.65 |
Cytoplasmic Foci | 1.85 | 1.89 | -1.13 | -1.95 | -1.96 | -0.85 | 0.14 | 0.27 | 1.15 | 0.27 | 0.56 | 1.68 | -1.50 | -1.67 | -1.91 |
Eisosomes | -3.31 | -6.67 | -3.99 | -0.77 | 4.89 | 0.39 | -5.78 | -0.62 | -0.78 | 2.73 | -1.93 | -8.55 | -2.46 | -0.59 | 7.18 |
Endoplasmic Reticulum | -0.90 | -3.20 | -10.73 | -8.70 | -3.39 | -4.55 | -8.22 | -2.49 | -0.81 | 2.24 | -4.27 | -7.74 | -10.76 | -7.16 | -1.15 |
Endosome | 1.19 | 1.69 | -1.64 | -2.92 | -3.34 | -1.25 | 0.94 | -0.06 | 1.25 | -1.14 | -0.27 | 1.97 | -2.16 | -2.03 | -3.27 |
Golgi | 0.84 | 0.72 | -2.16 | -2.50 | -2.46 | -1.99 | -2.67 | -3.53 | -1.26 | -1.94 | -0.17 | -0.12 | -2.43 | -2.43 | -2.44 |
Lipid Particles | 1.11 | 0.93 | 0.38 | -4.48 | -3.29 | -1.17 | -6.77 | -1.50 | -0.57 | -0.75 | 0.11 | 0.12 | -0.67 | -0.95 | -2.08 |
Mitochondria | -3.04 | -9.32 | -8.53 | -7.04 | -5.52 | -1.83 | -3.84 | -4.61 | -2.02 | -1.94 | -2.78 | -7.50 | -8.31 | -6.66 | -5.89 |
Mitotic Spindle | -0.31 | -0.82 | -7.42 | -7.28 | -3.93 | -3.03 | -2.55 | -2.06 | -1.72 | -1.85 | -2.75 | -1.32 | -7.94 | -7.08 | -5.67 |
None | 4.98 | 5.62 | -0.20 | -4.81 | -5.38 | -4.84 | -2.24 | -4.27 | 1.42 | 0.75 | -2.65 | -1.04 | -3.19 | -0.24 | -0.58 |
Nuclear Periphery | 0.45 | -1.66 | -9.19 | -9.43 | -8.91 | -3.20 | -6.69 | -4.78 | -1.78 | -2.52 | -2.87 | -5.76 | -9.63 | -8.55 | -8.75 |
Nuclear Periphery Foci | 1.67 | 1.53 | -2.13 | -3.74 | -3.64 | -2.62 | -0.88 | -1.05 | -0.78 | -1.02 | -1.56 | 0.85 | -2.88 | -1.97 | -3.20 |
Nucleolus | 0.98 | 2.03 | 14.37 | 13.13 | 5.36 | -3.26 | 5.49 | 7.62 | 9.26 | 1.67 | -1.90 | 5.15 | 18.49 | 19.38 | 5.39 |
Nucleus | -0.92 | -0.30 | 7.15 | 7.82 | 5.34 | 6.43 | -0.77 | 0.64 | -1.23 | 0.95 | 4.49 | -0.67 | 7.95 | 5.34 | 6.60 |
Peroxisomes | -1.41 | -5.98 | 0.01 | 2.05 | 6.07 | -1.91 | -5.21 | -1.96 | -1.44 | 0.93 | -2.39 | -7.43 | -1.37 | 0.98 | 7.06 |
Vacuole | -0.90 | -2.27 | -5.91 | -5.69 | -5.46 | -1.40 | -1.82 | -2.45 | -2.05 | 0.33 | -1.67 | -2.09 | -6.17 | -5.81 | -3.20 |
Vacuole Periphery | -1.73 | 0.55 | -2.53 | -1.60 | -2.63 | -2.55 | 0.68 | 0.27 | 3.21 | -0.81 | -2.79 | 0.83 | -2.55 | -1.78 | -2.65 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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