Standard name
Human Ortholog
Description Ubiquitin conjugating enzyme; involved in the ER-associated protein degradation (ERAD) pathway and in the inner nuclear membrane-associated degradation (INMAD) pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.95 0.96 1.0 – 0.82 0.73 – 0.57 0.58 0.66 0.39 0.46 0.97 1.0 0.98 0.78 0.77 0.85 0.76 0.72 0.57 0.73 0.71 0.72
Endoplasmic Reticulum 0 0 0 – 0 0 – 0.06 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.11 – 0.1 0.38 – 0.54 0.55 0.49 0.65 0.72 0 0 0 0 0.07 0 0.08 0.09 0.21 0.1 0.08 0.06
Nucleus 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 – 0.06 0.06 – 0 0 0 0 0 0 0 0 0.15 0.11 0.09 0 0 0.07 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 – 0 0 0 1 0 0 0 0 2 2 0 2 2 1 2 0 3
Bud 0 1 0 – 0 0 – 3 3 1 4 6 0 0 0 1 1 0 7 3 0 2 1 4
Bud Neck 0 0 0 – 0 0 – 1 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 1
Bud Site 0 0 0 – 0 0 – 0 1 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 1 0 – 0 1 – 2 0 0 2 3 1 2 2 10 11 8 1 2 1 0 1 1
Cytoplasm 254 146 19 – 42 77 – 72 60 55 47 55 169 135 205 218 150 232 199 128 32 101 96 104
Endoplasmic Reticulum 0 0 0 – 2 3 – 7 2 5 9 4 4 0 3 11 8 12 8 6 1 4 3 4
Endosome 2 0 0 – 0 1 – 0 0 0 0 0 0 0 0 7 6 9 2 3 1 0 2 1
Golgi 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1 3 1
Mitochondria 11 2 2 – 5 40 – 68 57 41 79 86 1 0 2 8 14 12 19 16 12 13 11 9
Nucleus 0 1 0 – 0 0 – 2 1 0 1 3 0 0 1 2 3 2 0 2 0 0 0 0
Nuclear Periphery 0 0 0 – 0 1 – 1 3 1 1 0 0 0 1 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 – 1 0 – 0 1 0 1 0 0 0 0 1 1 0 0 0 0 1 0 0
Peroxisomes 1 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 1 – 0 1 0 2 1 0 0 0 2 0 1 2 1 0 0 1 0
Vac/Vac Membrane 7 5 0 – 3 6 – 1 0 1 1 2 1 0 0 43 21 24 8 3 4 5 7 5
Unique Cell Count 268 152 19 51 106 127 104 83 121 119 175 135 209 279 194 274 261 179 57 138 136 145
Labelled Cell Count 279 156 21 53 130 157 129 104 149 162 177 137 214 305 217 301 261 179 57 138 136 145


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.7 3.1 – 5.2 3.7 – 3.5 3.2 3.7 3.3 3.2 4.5 4.6 4.7 6.7 6.7 6.4 4.4 5.3 4.9
Std Deviation (1e-4) 2.3 1.4 0.8 – 2.5 1.7 – 1.3 1.4 1.3 0.9 0.8 1.1 0.7 1.3 1.9 1.4 1.9 1.7 2.3 1.6
Intensity Change (Log2) – – – – 0.77 0.27 – 0.18 0.07 0.28 0.12 0.07 0.56 0.59 0.63 1.13 1.13 1.07 0.54 0.78 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1445 0.113 -0.0616 -0.4108 -0.215 -0.0884 -1.1983 -1.1447 -1.0965 -0.7878 -0.2736 -0.9352 0.774 0.5409 0.6084 1.1263 1.4261 0.5784
Actin 0.014 0.0001 0.0066 0.0408 0.0042 0.0047 0.0116 0.0003 0.0037 0.0013 0.0107 0.0153 0.0204 0.0002 0.0018 0.0279 0.0475 0.0014
Bud 0.0014 0 0.0001 0.0002 0.0004 0.0005 0.0017 0.0104 0.002 0.0007 0.0293 0.0038 0.0008 0.003 0.0006 0.0014 0.0004 0.0003
Bud Neck 0.0021 0.0001 0.0001 0.0003 0.0006 0.0009 0.0048 0.0001 0.0011 0.0091 0.0045 0.0116 0.0117 0.0002 0.0018 0.0029 0.0008 0.0009
Bud Periphery 0.0011 0 0.0001 0.0002 0.001 0.0009 0.0035 0.004 0.0014 0.0009 0.0161 0.0022 0.0009 0.0008 0.0007 0.0026 0.0012 0.0005
Bud Site 0.0016 0.0036 0.0005 0.0008 0.0002 0.0002 0.0109 0.0169 0.0022 0.0085 0.0107 0.0003 0.0115 0.0042 0.0008 0.0111 0.0006 0.0001
Cell Periphery 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0011 0.0007 0.0002 0.0004 0.0009 0.0002 0.0004 0.0003 0.0001 0.0003 0.0003 0.0001
Cytoplasm 0.0702 0.1086 0.1286 0.1371 0.1376 0.2081 0.0601 0.0955 0.053 0.088 0.0693 0.1071 0.0694 0.0821 0.09 0.0811 0.0485 0.1193
Cytoplasmic Foci 0.0297 0.002 0.0202 0.0149 0.0061 0.0076 0.0309 0.0059 0.0098 0.0223 0.0128 0.0142 0.0146 0.0034 0.0034 0.0152 0.0074 0.0074
Eisosomes 0.0002 0.0002 0.0002 0.0002 0.0003 0.0001 0.0005 0.0002 0.0004 0.0003 0.0003 0.0001 0.0003 0.0001 0.0001 0.0004 0.0011 0.0001
Endoplasmic Reticulum 0.0066 0.0019 0.0047 0.022 0.0114 0.0078 0.0094 0.0063 0.0022 0.0044 0.0054 0.0056 0.0095 0.0014 0.0145 0.0195 0.0059 0.0072
Endosome 0.0231 0.0004 0.009 0.0016 0.0221 0.0121 0.017 0.001 0.0055 0.0428 0.0299 0.0093 0.0161 0.0005 0.002 0.0129 0.0251 0.0022
Golgi 0.0073 0 0.0048 0.0029 0.0025 0.0086 0.0065 0.0001 0.007 0.0071 0.0147 0.0027 0.0042 0 0.0004 0.0036 0.0101 0.0003
Lipid Particles 0.0187 0.0002 0.0086 0.0039 0.002 0.0111 0.0271 0.0004 0.0057 0.0086 0.0399 0.0129 0.0046 0.0002 0.0015 0.0051 0.025 0.0006
Mitochondria 0.0082 0.0001 0.0007 0.0012 0.0362 0.0074 0.0124 0.0016 0.0058 0.0015 0.0161 0.0007 0.0019 0.0003 0.0011 0.001 0.0097 0.0004
None 0.7792 0.8807 0.81 0.7485 0.7536 0.7183 0.7554 0.8508 0.8723 0.7917 0.6291 0.7984 0.7942 0.8936 0.8651 0.7871 0.7476 0.8455
Nuclear Periphery 0.0046 0.0003 0.0005 0.001 0.0105 0.0019 0.0091 0.0009 0.0011 0.0009 0.0567 0.004 0.0156 0.0006 0.0021 0.0036 0.0471 0.0046
Nucleolus 0.0015 0.0001 0.0002 0.0001 0.0005 0.0001 0.0015 0.0004 0.0009 0.0007 0.0014 0.0007 0.0014 0.001 0.0016 0.0009 0.0011 0.0002
Nucleus 0.0145 0.0008 0.0005 0.0005 0.0034 0.0009 0.0022 0.0022 0.0038 0.0013 0.0161 0.0033 0.0054 0.004 0.0052 0.0014 0.0035 0.0024
Peroxisomes 0.0071 0 0.0017 0.0059 0.0004 0.0053 0.0123 0.0001 0.0059 0.0033 0.0025 0.001 0.0059 0.0001 0.0034 0.0092 0.0068 0.0002
Punctate Nuclear 0.0055 0.0005 0.0022 0.0172 0.0026 0.0021 0.0024 0.0013 0.0125 0.0026 0.0015 0.0049 0.0082 0.003 0.0024 0.0117 0.005 0.0053
Vacuole 0.0022 0.0003 0.0006 0.0005 0.0025 0.0008 0.0158 0.0008 0.0028 0.0033 0.0138 0.0013 0.0017 0.0009 0.001 0.0008 0.0017 0.0007
Vacuole Periphery 0.001 0 0.0001 0.0001 0.0017 0.0005 0.0037 0.0001 0.0006 0.0007 0.0182 0.0004 0.0011 0.0001 0.0002 0.0003 0.0038 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 67.578 21.7874 41.1529 50.959 60.327 44.2889 39.5525 31.764 54.3278 56.3066
Translational Efficiency 2.2449 3.5565 1.7778 1.4897 1.8695 3.5031 2.4275 2.4678 1.6826 1.9604

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1411 1209 167 645 1888 115 1880 1200 3299 1324 2047 1845

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 669.01 704.12 820.55 1090.07 618.80 738.03 770.52 783.96 640.28 707.07 774.60 890.97
Standard Deviation 75.92 87.87 93.69 1277.70 76.87 92.56 88.78 107.39 80.40 88.81 90.24 774.29
Intensity Change Log 2 — 0.073794 0.294563 0.704321 — 0.254206 0.316359 0.341307 — 0.163301 0.305078 0.541224

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000138 0.001485 0.000392 0.004832 0.000144 0.000158 0.000218 0.000751 0.000142 0.001369 0.000232 0.002177
Bud Neck 0.018138 0.067473 0.002356 0.007247 0.020976 0.038953 0.013282 0.009588 0.019762 0.064996 0.012391 0.008769
Bud Site 0.003287 0.029041 0.013356 0.019445 0.004376 0.004224 0.020469 0.040167 0.003910 0.026886 0.019888 0.032923
Cell Periphery 0.000229 0.000432 0.000268 0.000733 0.000217 0.000273 0.000142 0.000219 0.000222 0.000419 0.000152 0.000399
Cytoplasm 0.466656* 0.249482* 0.088195 0.340867* 0.396267* 0.281729* 0.357446* 0.457221* 0.426373* 0.252283* 0.335480* 0.416545*
Cytoplasmic Foci 0.152927 0.301103* 0.024741 0.043359 0.244366* 0.359325* 0.008301 0.024098 0.205257* 0.306160* 0.009643 0.030831
Eisosomes 0.000292 0.000367 0.000136 0.000363 0.000245 0.000378 0.000029 0.000053 0.000265 0.000368 0.000038 0.000161
Endoplasmic Reticulum 0.027897 0.003654 0.006977 0.001834 0.003879 0.000478 0.001897 0.001811 0.014152 0.003378 0.002311 0.001819
Endosome 0.010726 0.051586 0.003810 0.011594 0.013822 0.054546 0.001396 0.010598 0.012498 0.051843 0.001593 0.010946
Golgi 0.002548 0.018083 0.000095 0.011987 0.003486 0.008839 0.000054 0.006536 0.003085 0.017280 0.000058 0.008442
Lipid Particles 0.026313 0.025655 0.006365 0.002998 0.029559 0.021955 0.000932 0.001816 0.028171 0.025333 0.001375 0.002229
Mitochondria 0.003622 0.044977 0.000445 0.137006* 0.012387 0.014593 0.001027 0.005812 0.008638 0.042338 0.000979 0.051676
Mitotic Spindle 0.000625 0.012316 0.000051 0.034111 0.000730 0.001512 0.005772 0.025132 0.000685 0.011378 0.005306 0.028271
None 0.003865 0.004004 0.002597 0.005614 0.004098 0.000908 0.004023 0.014020 0.003998 0.003735 0.003907 0.011081
Nuclear Periphery 0.002211 0.001091 0.002204 0.001584 0.001734 0.000739 0.001656 0.001266 0.001938 0.001061 0.001700 0.001377
Nuclear Periphery Foci 0.001381 0.001142 0.002245 0.000864 0.000738 0.000307 0.000475 0.000692 0.001013 0.001069 0.000619 0.000752
Nucleolus 0.001013 0.002550 0.002079 0.001522 0.002402 0.002719 0.000415 0.001501 0.001808 0.002565 0.000550 0.001508
Nucleus 0.218630* 0.074530 0.784866* 0.242304* 0.206463* 0.123417 0.545839* 0.288838* 0.211667* 0.078776 0.565339* 0.272570*
Peroxisomes 0.004250 0.046817 0.000771 0.005007 0.008828 0.021976 0.000285 0.005044 0.006870 0.044659 0.000324 0.005031
Vacuole 0.054572 0.059578 0.057807 0.116392 0.043972 0.059427 0.036074 0.102623 0.048506 0.059564 0.037847 0.107436
Vacuole Periphery 0.000682 0.004634 0.000245 0.010336 0.001309 0.003546 0.000270 0.002215 0.001041 0.004540 0.000268 0.005054

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.33 -5.87 -2.44 -0.94 -2.10 -0.27 -2.08 -4.29 -3.80 -3.25 -2.32 -3.02 -3.12 0.21 -2.82
Bud Neck -11.29 10.25 7.42 14.65 -2.55 -1.83 4.81 8.60 3.23 2.84 -11.35 5.57 11.21 14.88 3.20
Bud Site -9.78 -1.30 -4.39 3.92 -0.60 0.14 -5.87 -5.76 -5.55 -1.42 -9.65 -6.27 -7.14 2.49 -1.13
Cell Periphery -5.11 -1.84 -3.20 -1.19 -2.62 -1.06 1.78 1.43 3.73 -0.84 -4.52 2.73 -1.55 2.45 -3.82
Cytoplasm 22.78 31.34 12.59 -3.98 -14.08 4.97 9.25 4.70 -2.89 -3.02 21.53 17.65 11.80 -8.48 -3.82
Cytoplasmic Foci -18.70 21.32 22.51 37.65 -1.80 -5.43 49.53 46.25 16.45 -6.55 -14.17 56.44 49.87 44.45 -9.38
Eisosomes -2.82 5.93 2.21 4.66 -2.29 -3.18 17.97 15.22 7.72 -11.06 -5.16 20.50 10.39 12.98 -8.01
Endoplasmic Reticulum 7.46 5.76 7.90 1.08 5.97 6.59 2.30 1.43 -11.09 -1.80 6.93 7.79 7.92 0.54 0.67
Endosome -13.79 5.53 2.31 14.39 -1.57 -3.36 14.65 7.35 4.09 -4.53 -13.93 16.53 6.99 16.23 -5.58
Golgi -10.60 7.78 -1.66 5.65 -3.12 -1.20 11.23 0.92 1.40 -2.33 -10.28 13.71 -0.46 8.39 -3.82
Lipid Particles 0.21 9.74 12.45 13.03 5.65 1.69 16.10 15.61 4.87 -3.67 1.31 20.57 20.03 14.21 -3.13
Mitochondria -12.91 11.17 -6.81 -1.99 -7.18 -0.34 9.19 6.35 2.10 -1.81 -11.06 10.51 -4.68 4.20 -7.17
Mitotic Spindle -5.36 1.14 -4.75 -1.92 -4.90 -0.54 -4.81 -6.56 -5.88 -4.22 -5.40 -4.87 -8.07 -2.72 -5.47
None -0.27 2.47 -0.11 0.19 -2.60 5.25 0.23 -2.33 -4.61 -2.57 0.18 0.37 -2.34 -2.06 -2.62
Nuclear Periphery 2.44 -0.85 -0.14 -3.83 1.24 3.47 -2.36 -2.98 -9.59 -1.39 2.76 -1.88 -2.16 -5.45 -0.63
Nuclear Periphery Foci 0.66 -2.37 1.06 0.22 3.12 3.52 1.49 -1.18 -7.04 -3.76 -0.19 2.00 0.25 0.37 -2.66
Nucleolus -3.46 -3.08 0.44 3.47 3.01 -0.73 5.70 3.31 2.60 -1.55 -1.70 6.19 3.17 3.68 -1.76
Nucleus 24.61 -26.97 -5.94 -18.51 20.89 5.41 -44.79 -16.24 -14.17 19.88 27.68 -51.58 -16.64 -34.13 27.02
Peroxisomes -14.59 5.88 1.28 14.61 -2.15 -3.56 11.98 6.24 5.40 -2.28 -14.08 13.64 5.76 15.62 -3.32
Vacuole -1.32 -3.05 -16.20 -15.54 -9.83 -1.59 -3.72 -20.85 -11.49 -18.57 -3.54 -3.34 -26.22 -22.78 -23.20
Vacuole Periphery -9.54 7.45 -3.52 -0.14 -3.89 -1.30 10.41 1.21 1.51 -2.26 -8.59 11.83 -2.68 3.71 -4.49
Standard name
Human Ortholog
Description Ubiquitin conjugating enzyme; involved in the ER-associated protein degradation (ERAD) pathway and in the inner nuclear membrane-associated degradation (INMAD) pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
Localization
Cell Percentages cytoplasm (20%), ER (12%), mixed (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ubc7

Ubc7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubc7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available