Standard name | |
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Human Ortholog | |
Description | Ubiquitin conjugating enzyme; involved in the ER-associated protein degradation (ERAD) pathway and in the inner nuclear membrane-associated degradation (INMAD) pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 2 | 2 | 1 | 2 | 0 | 3 |
Bud | 0 | 1 | 0 | – | 0 | 0 | – | 3 | 3 | 1 | 4 | 6 | 0 | 0 | 0 | 1 | 1 | 0 | 7 | 3 | 0 | 2 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 1 | 0 | – | 0 | 1 | – | 2 | 0 | 0 | 2 | 3 | 1 | 2 | 2 | 10 | 11 | 8 | 1 | 2 | 1 | 0 | 1 | 1 |
Cytoplasm | 254 | 146 | 19 | – | 42 | 77 | – | 72 | 60 | 55 | 47 | 55 | 169 | 135 | 205 | 218 | 150 | 232 | 199 | 128 | 32 | 101 | 96 | 104 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 2 | 3 | – | 7 | 2 | 5 | 9 | 4 | 4 | 0 | 3 | 11 | 8 | 12 | 8 | 6 | 1 | 4 | 3 | 4 |
Endosome | 2 | 0 | 0 | – | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 6 | 9 | 2 | 3 | 1 | 0 | 2 | 1 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 3 | 1 |
Mitochondria | 11 | 2 | 2 | – | 5 | 40 | – | 68 | 57 | 41 | 79 | 86 | 1 | 0 | 2 | 8 | 14 | 12 | 19 | 16 | 12 | 13 | 11 | 9 |
Nucleus | 0 | 1 | 0 | – | 0 | 0 | – | 2 | 1 | 0 | 1 | 3 | 0 | 0 | 1 | 2 | 3 | 2 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 1 | – | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | – | 1 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 1 | – | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 7 | 5 | 0 | – | 3 | 6 | – | 1 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 43 | 21 | 24 | 8 | 3 | 4 | 5 | 7 | 5 |
Unique Cell Count | 268 | 152 | 19 | 51 | 106 | 127 | 104 | 83 | 121 | 119 | 175 | 135 | 209 | 279 | 194 | 274 | 261 | 179 | 57 | 138 | 136 | 145 | ||
Labelled Cell Count | 279 | 156 | 21 | 53 | 130 | 157 | 129 | 104 | 149 | 162 | 177 | 137 | 214 | 305 | 217 | 301 | 261 | 179 | 57 | 138 | 136 | 145 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 4.7 | 3.1 | – | 5.2 | 3.7 | – | 3.5 | 3.2 | 3.7 | 3.3 | 3.2 | 4.5 | 4.6 | 4.7 | 6.7 | 6.7 | 6.4 | 4.4 | 5.3 | 4.9 |
Std Deviation (1e-4) | 2.3 | 1.4 | 0.8 | – | 2.5 | 1.7 | – | 1.3 | 1.4 | 1.3 | 0.9 | 0.8 | 1.1 | 0.7 | 1.3 | 1.9 | 1.4 | 1.9 | 1.7 | 2.3 | 1.6 |
Intensity Change (Log2) | – | – | – | – | 0.77 | 0.27 | – | 0.18 | 0.07 | 0.28 | 0.12 | 0.07 | 0.56 | 0.59 | 0.63 | 1.13 | 1.13 | 1.07 | 0.54 | 0.78 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1445 | 0.113 | -0.0616 | -0.4108 | -0.215 | -0.0884 | -1.1983 | -1.1447 | -1.0965 | -0.7878 | -0.2736 | -0.9352 | 0.774 | 0.5409 | 0.6084 | 1.1263 | 1.4261 | 0.5784 |
Actin | 0.014 | 0.0001 | 0.0066 | 0.0408 | 0.0042 | 0.0047 | 0.0116 | 0.0003 | 0.0037 | 0.0013 | 0.0107 | 0.0153 | 0.0204 | 0.0002 | 0.0018 | 0.0279 | 0.0475 | 0.0014 |
Bud | 0.0014 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0017 | 0.0104 | 0.002 | 0.0007 | 0.0293 | 0.0038 | 0.0008 | 0.003 | 0.0006 | 0.0014 | 0.0004 | 0.0003 |
Bud Neck | 0.0021 | 0.0001 | 0.0001 | 0.0003 | 0.0006 | 0.0009 | 0.0048 | 0.0001 | 0.0011 | 0.0091 | 0.0045 | 0.0116 | 0.0117 | 0.0002 | 0.0018 | 0.0029 | 0.0008 | 0.0009 |
Bud Periphery | 0.0011 | 0 | 0.0001 | 0.0002 | 0.001 | 0.0009 | 0.0035 | 0.004 | 0.0014 | 0.0009 | 0.0161 | 0.0022 | 0.0009 | 0.0008 | 0.0007 | 0.0026 | 0.0012 | 0.0005 |
Bud Site | 0.0016 | 0.0036 | 0.0005 | 0.0008 | 0.0002 | 0.0002 | 0.0109 | 0.0169 | 0.0022 | 0.0085 | 0.0107 | 0.0003 | 0.0115 | 0.0042 | 0.0008 | 0.0111 | 0.0006 | 0.0001 |
Cell Periphery | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0011 | 0.0007 | 0.0002 | 0.0004 | 0.0009 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0001 |
Cytoplasm | 0.0702 | 0.1086 | 0.1286 | 0.1371 | 0.1376 | 0.2081 | 0.0601 | 0.0955 | 0.053 | 0.088 | 0.0693 | 0.1071 | 0.0694 | 0.0821 | 0.09 | 0.0811 | 0.0485 | 0.1193 |
Cytoplasmic Foci | 0.0297 | 0.002 | 0.0202 | 0.0149 | 0.0061 | 0.0076 | 0.0309 | 0.0059 | 0.0098 | 0.0223 | 0.0128 | 0.0142 | 0.0146 | 0.0034 | 0.0034 | 0.0152 | 0.0074 | 0.0074 |
Eisosomes | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0011 | 0.0001 |
Endoplasmic Reticulum | 0.0066 | 0.0019 | 0.0047 | 0.022 | 0.0114 | 0.0078 | 0.0094 | 0.0063 | 0.0022 | 0.0044 | 0.0054 | 0.0056 | 0.0095 | 0.0014 | 0.0145 | 0.0195 | 0.0059 | 0.0072 |
Endosome | 0.0231 | 0.0004 | 0.009 | 0.0016 | 0.0221 | 0.0121 | 0.017 | 0.001 | 0.0055 | 0.0428 | 0.0299 | 0.0093 | 0.0161 | 0.0005 | 0.002 | 0.0129 | 0.0251 | 0.0022 |
Golgi | 0.0073 | 0 | 0.0048 | 0.0029 | 0.0025 | 0.0086 | 0.0065 | 0.0001 | 0.007 | 0.0071 | 0.0147 | 0.0027 | 0.0042 | 0 | 0.0004 | 0.0036 | 0.0101 | 0.0003 |
Lipid Particles | 0.0187 | 0.0002 | 0.0086 | 0.0039 | 0.002 | 0.0111 | 0.0271 | 0.0004 | 0.0057 | 0.0086 | 0.0399 | 0.0129 | 0.0046 | 0.0002 | 0.0015 | 0.0051 | 0.025 | 0.0006 |
Mitochondria | 0.0082 | 0.0001 | 0.0007 | 0.0012 | 0.0362 | 0.0074 | 0.0124 | 0.0016 | 0.0058 | 0.0015 | 0.0161 | 0.0007 | 0.0019 | 0.0003 | 0.0011 | 0.001 | 0.0097 | 0.0004 |
None | 0.7792 | 0.8807 | 0.81 | 0.7485 | 0.7536 | 0.7183 | 0.7554 | 0.8508 | 0.8723 | 0.7917 | 0.6291 | 0.7984 | 0.7942 | 0.8936 | 0.8651 | 0.7871 | 0.7476 | 0.8455 |
Nuclear Periphery | 0.0046 | 0.0003 | 0.0005 | 0.001 | 0.0105 | 0.0019 | 0.0091 | 0.0009 | 0.0011 | 0.0009 | 0.0567 | 0.004 | 0.0156 | 0.0006 | 0.0021 | 0.0036 | 0.0471 | 0.0046 |
Nucleolus | 0.0015 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0015 | 0.0004 | 0.0009 | 0.0007 | 0.0014 | 0.0007 | 0.0014 | 0.001 | 0.0016 | 0.0009 | 0.0011 | 0.0002 |
Nucleus | 0.0145 | 0.0008 | 0.0005 | 0.0005 | 0.0034 | 0.0009 | 0.0022 | 0.0022 | 0.0038 | 0.0013 | 0.0161 | 0.0033 | 0.0054 | 0.004 | 0.0052 | 0.0014 | 0.0035 | 0.0024 |
Peroxisomes | 0.0071 | 0 | 0.0017 | 0.0059 | 0.0004 | 0.0053 | 0.0123 | 0.0001 | 0.0059 | 0.0033 | 0.0025 | 0.001 | 0.0059 | 0.0001 | 0.0034 | 0.0092 | 0.0068 | 0.0002 |
Punctate Nuclear | 0.0055 | 0.0005 | 0.0022 | 0.0172 | 0.0026 | 0.0021 | 0.0024 | 0.0013 | 0.0125 | 0.0026 | 0.0015 | 0.0049 | 0.0082 | 0.003 | 0.0024 | 0.0117 | 0.005 | 0.0053 |
Vacuole | 0.0022 | 0.0003 | 0.0006 | 0.0005 | 0.0025 | 0.0008 | 0.0158 | 0.0008 | 0.0028 | 0.0033 | 0.0138 | 0.0013 | 0.0017 | 0.0009 | 0.001 | 0.0008 | 0.0017 | 0.0007 |
Vacuole Periphery | 0.001 | 0 | 0.0001 | 0.0001 | 0.0017 | 0.0005 | 0.0037 | 0.0001 | 0.0006 | 0.0007 | 0.0182 | 0.0004 | 0.0011 | 0.0001 | 0.0002 | 0.0003 | 0.0038 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 67.578 | 21.7874 | 41.1529 | 50.959 | 60.327 | 44.2889 | 39.5525 | 31.764 | 54.3278 | 56.3066 |
Translational Efficiency | 2.2449 | 3.5565 | 1.7778 | 1.4897 | 1.8695 | 3.5031 | 2.4275 | 2.4678 | 1.6826 | 1.9604 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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