ORF
Human Ortholog
Description A metal-dependent phosphatase, part of the DUF89 protein family; dephosphorylates fructose-1-phosphate; human ortholog, C6orf211 is involved in response to DNA damage; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 0.97 0.98 0.97 0.93 0.84 0.91 0.85 0.8 0.8 0.99 1.0 1.0 0.96 0.98 0.95 0.97 0.96 0.97 0.98 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0.06 0.1 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 2 0 2 1 2 7 1 7 10 9 1 3 2 3 9 5 0 0 0 0 0 0
Cytoplasm 62 464 146 75 131 124 167 153 102 144 126 129 359 452 490 353 338 200 86 437 133 225 314 313
Endoplasmic Reticulum 0 3 1 0 1 0 7 4 3 6 5 3 1 1 0 13 12 6 0 1 1 0 0 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Golgi 0 2 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0
Mitochondria 0 1 0 2 0 3 0 8 6 11 15 23 0 0 1 2 1 1 0 3 0 0 1 0
Nucleus 0 2 0 1 0 1 2 5 1 4 2 2 0 0 1 4 0 1 0 3 0 1 2 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 0 2 15 1 5 9 6 1 0 0 15 13 8 0 4 0 0 1 3
Unique Cell Count 62 468 146 77 134 128 179 183 112 170 157 162 361 454 490 368 345 210 89 456 138 230 327 330
Labelled Cell Count 62 477 149 78 136 129 181 193 116 177 168 173 362 457 494 391 374 222 89 456 138 230 327 330


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.4 19.1 17.1 15.8 17.4 16.0 15.8 14.8 15.0 14.3 14.2 14.3 21.9 22.3 22.0 20.7 23.6 25.1 15.7 15.9 17.4
Std Deviation (1e-4) 2.4 3.5 2.9 5.0 4.1 4.6 4.3 4.6 3.8 3.8 3.5 4.0 4.1 4.0 4.0 5.2 6.8 7.1 3.4 3.3 3.7
Intensity Change (Log2) – – – -0.12 0.02 -0.1 -0.12 -0.21 -0.19 -0.26 -0.28 -0.26 0.35 0.38 0.36 0.27 0.46 0.55 -0.13 -0.11 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 2.2 2.0 0 0 0 0 0 0
Cytoplasm -2.0 -1.8 -2.2 -3.2 -5.1 -3.7 -4.9 -5.7 -5.8 -0.9 -0.8 0 -2.5 -1.7 -2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.1 3.8 4.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.5 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.8516 16.552 15.2838 14.2652 13.731 14.5072 11.3937 16.0642 13.122 13.008 12.746 13.8649 14.9117 18.1033 16.3767 17.3415 15.6625 16.6507
Actin 0.0121 0.0004 0.0012 0.0007 0.0016 0.0058 0.0391 0.0039 0.0217 0.0176 0.0368 0.0065 0.0181 0.0003 0.0019 0.0004 0.0155 0.0004
Bud 0.0009 0.0001 0.0001 0.0002 0.0001 0.0002 0.0005 0.0069 0.0004 0.0005 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0022 0.0002 0.0003 0.0242 0.0014 0.001 0.0013 0.0002 0.0007 0.0025 0.0006 0.0009 0.0026 0.0002 0.0004 0.001 0.0006 0.0011
Bud Periphery 0.0015 0 0 0.0006 0 0.0001 0.0003 0.0016 0.0005 0.0006 0.0001 0.0001 0.0002 0 0.0001 0 0 0
Bud Site 0.0016 0.0003 0.0002 0.0014 0.0002 0.0002 0.0019 0.0095 0.0109 0.007 0.0003 0.0001 0.0012 0.0001 0.0001 0 0.0001 0
Cell Periphery 0.0011 0 0 0.003 0.0001 0.0001 0.0001 0.0001 0.0005 0.0008 0 0 0.0002 0 0 0 0 0
Cytoplasm 0.8863 0.9825 0.9754 0.9428 0.8802 0.9351 0.7858 0.9571 0.9168 0.8949 0.9251 0.9508 0.8919 0.9914 0.9522 0.9832 0.9482 0.9763
Cytoplasmic Foci 0.0036 0.0007 0.0022 0.0008 0.0017 0.0021 0.0314 0.0016 0.0107 0.0129 0.0048 0.0029 0.0069 0.0006 0.0092 0.0034 0.0119 0.0016
Eisosomes 0.0004 0 0 0.0006 0 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.0072 0.0009 0.0008 0.0005 0.0074 0.0101 0.0062 0.0014 0.0041 0.0015 0.0009 0.0018 0.0059 0.0005 0.0014 0.0004 0.0018 0.0013
Endosome 0.0102 0.0003 0.0008 0.0007 0.0129 0.005 0.0412 0.0027 0.0111 0.0175 0.002 0.0086 0.0103 0.0002 0.0076 0.0002 0.0023 0.0006
Golgi 0.0024 0 0.0003 0.0002 0.0008 0.0006 0.0216 0.0006 0.003 0.0083 0.0046 0.0038 0.0027 0 0.001 0 0.0018 0
Lipid Particles 0.0018 0 0.0015 0.0004 0.0019 0.0005 0.0133 0.0006 0.0021 0.0069 0.0015 0.0021 0.0085 0 0.0007 0 0.0042 0
Mitochondria 0.005 0.0001 0.0011 0.0004 0.0003 0.0012 0.0037 0.003 0.001 0.0028 0.0016 0.0012 0.0012 0.0001 0.0005 0.0001 0.0002 0.0001
None 0.004 0.0004 0.0008 0.0009 0.0011 0.0012 0.011 0.0016 0.0029 0.0015 0.0006 0.0013 0.0116 0.0006 0.0021 0.0008 0.001 0.001
Nuclear Periphery 0.0105 0.0015 0.0013 0.0018 0.0225 0.0086 0.0063 0.002 0.0045 0.0012 0.0017 0.0049 0.0096 0.0006 0.0028 0.0009 0.0011 0.0017
Nucleolus 0.0035 0 0 0.0008 0.0002 0.0001 0.0003 0.0003 0.0001 0.0026 0 0 0.0014 0 0.0002 0 0 0
Nucleus 0.0345 0.0116 0.0115 0.0183 0.0614 0.0246 0.0179 0.0044 0.0051 0.0077 0.0106 0.0118 0.0153 0.0052 0.0105 0.0087 0.0086 0.0148
Peroxisomes 0.0006 0 0.0014 0.0003 0.0001 0.0002 0.0078 0.0005 0.0004 0.0015 0.0077 0.0014 0.0018 0 0.0006 0.0001 0.0013 0
Punctate Nuclear 0.005 0.0003 0.0003 0.0002 0.0032 0.0011 0.003 0.001 0.0018 0.0019 0.0005 0.0012 0.0086 0.0002 0.008 0.0005 0.0011 0.0007
Vacuole 0.0041 0.0004 0.0004 0.0011 0.0023 0.0018 0.0055 0.0005 0.0012 0.0089 0.0002 0.0004 0.001 0.0001 0.0004 0.0001 0.0002 0.0002
Vacuole Periphery 0.0015 0.0001 0.0001 0.0002 0.0006 0.0006 0.0015 0.0002 0.0006 0.0008 0.0001 0.0003 0.0005 0 0.0001 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 74.185 45.3747 72.52 76.5408 75.9348 80.3876 82.0516 86.9395 103.9428 89.7997
Translational Efficiency 1.5319 2.1454 1.2229 1.5484 1.2857 1.7825 1.5044 1.3066 1.3169 1.4749

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1537 1358 1928 2038 1868 1794 117 1770 3405 3152 2045 3808

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1231.16 1350.71 1999.01 1538.85 1535.99 1350.78 2037.60 1462.42 1398.39 1350.75 2001.22 1503.32
Standard Deviation 186.82 249.01 321.11 301.37 265.54 263.89 320.79 207.95 278.29 257.58 321.21 264.88
Intensity Change Log 2 — 0.133700 0.699267 0.321834 — -0.185376 0.407702 -0.070811 — -0.034646 0.544743 0.117172

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000070 0.000371 0.000108 0.000349 0.000117 0.000247 0.000772 0.000088 0.000096 0.000300 0.000146 0.000228
Bud Neck 0.000241 0.000263 0.000940 0.000686 0.000189 0.000338 0.002859 0.000465 0.000213 0.000306 0.001050 0.000584
Bud Site 0.001258 0.001834 0.001079 0.003286 0.000553 0.002568 0.009307 0.001521 0.000871 0.002252 0.001549 0.002466
Cell Periphery 0.000039 0.000044 0.000086 0.000096 0.000050 0.000052 0.000313 0.000036 0.000045 0.000048 0.000099 0.000068
Cytoplasm 0.870881* 0.864342* 0.674345* 0.850459* 0.814639* 0.870499* 0.573348* 0.866928* 0.840027* 0.867846* 0.668566* 0.858114*
Cytoplasmic Foci 0.012377 0.006004 0.004404 0.007238 0.003569 0.013044 0.017275 0.006442 0.007545 0.010011 0.005141 0.006868
Eisosomes 0.000002 0.000001 0.000001 0.000002 0.000001 0.000003 0.000009 0.000001 0.000001 0.000002 0.000002 0.000002
Endoplasmic Reticulum 0.000301 0.000124 0.000235 0.000123 0.000192 0.000441 0.000883 0.000127 0.000241 0.000305 0.000272 0.000125
Endosome 0.000308 0.000205 0.000441 0.000698 0.000092 0.001384 0.004101 0.001012 0.000189 0.000876 0.000650 0.000844
Golgi 0.000246 0.000193 0.000241 0.001097 0.000215 0.003347 0.005690 0.000636 0.000229 0.001988 0.000553 0.000883
Lipid Particles 0.000139 0.000122 0.000309 0.000465 0.000117 0.000446 0.001762 0.000214 0.000127 0.000307 0.000392 0.000348
Mitochondria 0.000101 0.000043 0.000614 0.000077 0.000091 0.000733 0.000749 0.000133 0.000096 0.000436 0.000621 0.000103
Mitotic Spindle 0.000957 0.001263 0.002996 0.004366 0.000351 0.004737 0.034477 0.005850 0.000624 0.003241 0.004797 0.005056
None 0.020045 0.016513 0.011280 0.005405 0.013661 0.011964 0.003759 0.004267 0.016543 0.013924 0.010849 0.004876
Nuclear Periphery 0.000045 0.000044 0.000317 0.000077 0.000042 0.000054 0.000789 0.000141 0.000043 0.000050 0.000344 0.000107
Nuclear Periphery Foci 0.001975 0.002185 0.000947 0.001637 0.001348 0.002427 0.005770 0.001339 0.001631 0.002323 0.001223 0.001499
Nucleolus 0.000079 0.000087 0.000138 0.000072 0.000116 0.000291 0.000169 0.000108 0.000099 0.000203 0.000140 0.000089
Nucleus 0.084939 0.099085 0.291203* 0.113098 0.161609* 0.076993 0.252613* 0.095366 0.127000 0.086511 0.288995* 0.104856
Peroxisomes 0.000070 0.000043 0.000080 0.000106 0.000037 0.000603 0.000135 0.000048 0.000052 0.000361 0.000083 0.000079
Vacuole 0.005839 0.007115 0.010020 0.010507 0.002902 0.008892 0.083452 0.014996 0.004228 0.008126 0.014221 0.012594
Vacuole Periphery 0.000087 0.000120 0.000215 0.000156 0.000109 0.000933 0.001767 0.000282 0.000099 0.000583 0.000304 0.000214

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.25 -7.98 -3.55 -0.26 -2.15 -2.52 -6.06 1.07 2.93 6.20 -1.94 -7.95 -3.24 0.21 -0.42
Bud Neck -1.75 -9.57 -8.69 -7.80 4.14 -4.16 -9.32 -10.01 -6.21 8.44 -4.23 -11.63 -12.49 -9.76 6.92
Bud Site -1.37 -2.57 -6.40 -3.32 -5.06 -7.53 -7.48 -6.54 2.05 6.28 -5.55 -7.58 -8.79 -1.99 -2.67
Cell Periphery -2.39 -12.06 -9.63 -8.04 1.89 0.14 -8.06 2.15 1.42 8.24 -0.87 -13.01 -7.36 -5.83 8.18
Cytoplasm 2.96 45.33 13.19 9.66 -32.15 -10.32 16.76 -3.63 6.73 -17.55 -6.50 43.60 5.62 11.82 -39.45
Cytoplasmic Foci 5.56 6.52 3.24 -3.42 -4.68 -9.53 -7.25 -8.02 5.38 5.19 -4.07 1.14 -1.90 2.94 -3.01
Eisosomes 2.85 -3.15 -2.49 -4.42 -0.22 -5.08 -9.61 -3.79 3.31 9.09 -3.99 -8.75 -4.60 0.74 4.86
Endoplasmic Reticulum 3.19 0.50 3.38 0.44 2.74 -1.46 -4.82 1.59 1.93 5.42 -0.52 -0.99 3.62 1.99 3.51
Endosome 1.41 -1.43 -1.05 -1.58 -0.14 -4.20 -4.63 -2.76 1.08 2.62 -3.82 -3.64 -2.92 0.63 -0.42
Golgi 0.61 -0.39 -1.49 -1.71 -1.42 -3.65 -1.54 -1.24 3.23 1.25 -3.58 -1.71 -1.92 2.50 -1.24
Lipid Particles -0.60 -6.74 -8.38 -8.39 -1.18 -3.01 -7.52 -5.31 1.14 7.11 -3.07 -8.68 -9.61 -4.16 2.85
Mitochondria 1.25 -1.60 0.42 -7.10 1.71 -2.25 -3.18 -1.13 2.12 2.76 -2.05 -1.71 -0.17 2.04 1.70
Mitotic Spindle -0.72 -3.57 -2.85 -2.42 0.49 -3.34 -2.66 -5.40 -2.33 1.59 -3.26 -5.21 -6.15 -2.84 -0.48
None 2.54 6.90 10.46 8.02 15.06 2.12 8.41 11.40 7.75 -0.04 3.39 8.27 14.98 11.02 17.18
Nuclear Periphery -1.31 -5.24 -14.67 -11.78 4.34 -2.61 -12.48 -14.91 -12.42 10.85 -2.87 -6.08 -20.31 -16.34 4.69
Nuclear Periphery Foci -0.86 2.20 0.23 1.03 -1.94 -3.10 -8.02 -0.81 2.72 7.79 -2.86 -0.62 -0.50 2.53 0.13
Nucleolus -1.14 -5.65 -0.93 0.57 5.54 -1.27 -1.59 -0.23 1.49 4.49 -1.39 -1.81 -0.21 1.65 4.48
Nucleus -5.00 -48.33 -12.47 -6.54 37.70 21.48 -4.03 13.67 -9.09 7.33 14.04 -39.11 3.48 -11.80 42.75
Peroxisomes 0.73 -0.07 -0.51 -1.42 -0.37 -2.19 -2.97 -0.42 2.15 4.74 -2.08 -0.50 -0.74 1.91 0.04
Vacuole -4.15 -12.15 -14.66 -10.93 -1.01 -9.72 -11.14 -16.11 -9.87 9.75 -10.01 -15.42 -21.91 -14.61 3.54
Vacuole Periphery -0.38 -1.27 -0.58 -0.08 0.72 -2.44 -1.43 -1.33 2.04 1.04 -2.47 -1.43 -1.37 2.06 0.39
ORF
Human Ortholog
Description A metal-dependent phosphatase, part of the DUF89 protein family; dephosphorylates fructose-1-phosphate; human ortholog, C6orf211 is involved in response to DNA damage; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

YMR027W

YMR027W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr027w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available