Standard name
Human Ortholog
Description Protein involved in recovery from arrest in response to pheromone; acts in a cell cycle arrest recovery pathway independent from Far1p; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.13 0.15 0.19 0.13 0.08 0 0 0.05 0 0 0.05 0 – – – 0.3 0.36 0.22 0.1 0.07 0.22 0.07 0.06 0.11
Bud 0 0.05 0 0.05 0 0.05 0.06 0.05 0 0.05 0.09 0.06 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0.09 0.05 0 0 0 0 0 0.05 0.05 – – – 0.25 0.23 0.24 0 0 0 0 0 0.05
Cytoplasm 0.27 0.47 0.18 0.27 0.37 0.28 0.24 0.17 0.16 0.18 0.17 0.15 – – – 0.33 0.28 0.44 0.06 0.17 0 0 0 0
Endoplasmic Reticulum 0.59 0.29 0.44 0.28 0.22 0.19 0.12 0.16 0.24 0.17 0.24 0.18 – – – 0.06 0 0 0.47 0.37 0.5 0.36 0.39 0.4
Endosome 0 0 0 0 0 0.06 0.05 0.09 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0.06 0 0 0
Mitochondria 0.07 0.24 0.37 0.47 0.36 0.55 0.62 0.6 0.68 0.61 0.7 0.64 – – – 0.1 0.09 0.09 0.22 0.19 0.1 0.35 0.29 0.25
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.11 0.1 0.11 0.08 0.14 0.05 0.13 – – – 0 0.05 0 0 0.1 0 0.07 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 17 36 42 25 22 4 3 31 16 21 25 23 – – – 28 40 24 18 19 58 19 23 54
Bud 2 12 8 9 7 21 25 30 22 24 46 32 – – – 2 4 4 1 1 0 1 1 3
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 3 8 2 5 4 5
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 5 7 16 17 15 12 10 8 16 19 28 27 – – – 23 26 26 2 7 10 4 15 25
Cytoplasm 36 111 38 53 107 115 108 113 78 93 86 87 – – – 31 31 47 10 48 4 10 14 11
Endoplasmic Reticulum 80 69 95 55 65 79 52 104 118 88 124 105 – – – 6 3 4 84 101 132 93 143 196
Endosome 1 0 6 5 13 26 23 57 12 18 7 23 – – – 1 4 2 6 3 5 3 8 13
Golgi 1 0 3 0 2 0 0 1 1 1 1 0 – – – 1 4 1 5 3 17 9 11 15
Mitochondria 9 57 79 91 105 226 279 395 335 315 367 367 – – – 9 10 10 40 51 28 91 106 122
Nucleus 2 1 0 0 0 0 3 1 0 4 1 1 – – – 1 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 4 3 1 3 5 9 – – – 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 1 0 0 3 0 3 2 1 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 3 1 3 1 1 2 0 2 1 0 – – – 0 0 0 0 0 3 1 2 2
SpindlePole 1 0 0 0 1 0 1 1 0 0 0 1 – – – 1 3 1 0 0 0 0 1 0
Vac/Vac Membrane 2 1 6 6 21 44 46 75 39 73 26 72 – – – 4 6 3 5 27 3 17 26 28
Unique Cell Count 135 235 216 193 291 408 452 659 490 520 521 575 93 111 107 182 278 268 260 365 488
Labelled Cell Count 157 294 296 262 362 529 555 824 638 664 719 748 107 131 123 182 278 268 260 365 488


Ambiguous, Cytoplasm, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 5.5 6.7 6.5 6.5 5.8 5.9 6.3 6.1 6.1 6.1 6.1 – – – 7.7 7.7 7.7 6.5 6.9 6.8
Std Deviation (1e-4) 0.8 0.9 1.7 1.3 1.9 1.5 1.6 1.2 1.2 1.2 1.2 1.3 – – – 1.1 1.1 1.2 1.0 1.7 1.2
Intensity Change (Log2) – – – -0.05 -0.04 -0.21 -0.2 -0.09 -0.14 -0.14 -0.15 -0.14 – – – 0.19 0.19 0.2 -0.05 0.03 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.8 -4.0 -8.4 -9.1 -6.8 -7.2 -6.8 -6.3 -7.0 – – – 2.1 3.3 0.6
Bud 0.5 -0.9 0.8 1.0 0.5 0.5 0.6 2.4 1.1 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0.5 -1.0 -2.6 -3.2 -4.8 -2.4 -2.2 -1.1 -1.5 – – – 4.2 4.1 4.2
Cytoplasm 2.4 4.7 2.9 1.8 -0.2 -0.6 0.1 -0.4 -0.8 – – – 3.0 2.2 5.1
Endoplasmic Reticulum -3.2 -5.2 -6.5 -9.5 -8.6 -5.3 -7.7 -5.5 -7.4 – – – -6.5 -7.7 -7.4
Endosome -0.1 1.0 1.9 1.4 2.9 -0.3 0.5 0 0.8 – – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Mitochondria 2.2 -0.1 4.5 6.1 6.0 7.9 5.9 8.6 6.9 – – – -4.8 -5.3 -5.2
Nucleus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole 0.2 2.2 3.5 3.3 3.8 2.6 4.5 1.3 4.1 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9832 1.4294 1.3321 1.2015 0.8108 1.4802 1.7482 2.2368 2.1805 1.8791 2.354 2.0246 1.4747 2.441 1.6785 2.09 1.3297 1.6438
Actin 0.0508 0.0701 0.0726 0.0694 0.1102 0.0401 0.0772 0.0719 0.0959 0.1242 0.1176 0.0429 0.0491 0.0335 0.0574 0.0439 0.1593 0.068
Bud 0.0008 0.0019 0.0032 0.0031 0.0017 0.0034 0.0012 0.003 0.0057 0.0092 0.0012 0.0009 0.0009 0.0006 0.0025 0.0012 0.0042 0.0015
Bud Neck 0.0028 0.0024 0.0068 0.0111 0.0159 0.0353 0.0035 0.0031 0.0063 0.0193 0.0054 0.0279 0.004 0.0024 0.0047 0.0109 0.0194 0.0304
Bud Periphery 0.0016 0.0031 0.007 0.0268 0.0033 0.0031 0.0032 0.0043 0.0087 0.0218 0.0097 0.0031 0.0023 0.001 0.0037 0.0043 0.0109 0.0022
Bud Site 0.0103 0.0492 0.0223 0.0148 0.0016 0.0029 0.0099 0.0715 0.015 0.0043 0.0014 0.0015 0.0069 0.0445 0.021 0.0032 0.0031 0.0024
Cell Periphery 0.0101 0.0137 0.0082 0.0087 0.0035 0.0054 0.0124 0.0084 0.0107 0.0041 0.0071 0.0059 0.008 0.0104 0.005 0.0087 0.0104 0.0035
Cytoplasm 0.3104 0.3693 0.325 0.2401 0.3143 0.3425 0.2403 0.2713 0.3276 0.2912 0.2858 0.3237 0.248 0.4672 0.3626 0.3153 0.1711 0.3642
Cytoplasmic Foci 0.0551 0.0509 0.0692 0.0573 0.0639 0.0783 0.0754 0.0716 0.0702 0.058 0.0562 0.074 0.1002 0.0492 0.0674 0.0502 0.0312 0.0813
Eisosomes 0.0023 0.002 0.0029 0.0013 0.001 0.001 0.0026 0.0012 0.0017 0.0005 0.0016 0.0009 0.0014 0.0006 0.0008 0.0009 0.0025 0.0009
Endoplasmic Reticulum 0.2366 0.1186 0.1661 0.2546 0.1463 0.1696 0.1587 0.1654 0.1186 0.1799 0.0879 0.1877 0.1944 0.1479 0.1627 0.2243 0.1774 0.1744
Endosome 0.0971 0.1198 0.0979 0.1499 0.1462 0.1203 0.1159 0.1242 0.1095 0.1344 0.0848 0.1259 0.0949 0.0668 0.0743 0.0878 0.0848 0.0758
Golgi 0.069 0.061 0.0794 0.0558 0.1066 0.0812 0.0659 0.0941 0.0877 0.0637 0.0735 0.0716 0.0544 0.0403 0.0457 0.0418 0.0846 0.052
Lipid Particles 0.0283 0.0192 0.0183 0.0113 0.0248 0.0134 0.0464 0.0207 0.02 0.013 0.0352 0.0177 0.0582 0.061 0.0265 0.0285 0.027 0.0253
Mitochondria 0.011 0.0129 0.0293 0.0522 0.0279 0.0443 0.0242 0.0204 0.0416 0.016 0.0543 0.0343 0.0234 0.0079 0.0062 0.0601 0.1746 0.0359
None 0.089 0.0744 0.0653 0.0054 0.0133 0.0396 0.1144 0.0436 0.0501 0.0418 0.1409 0.0591 0.1066 0.0536 0.139 0.0925 0.0131 0.062
Nuclear Periphery 0.008 0.006 0.0054 0.0063 0.0034 0.0033 0.0096 0.0062 0.0044 0.0046 0.0035 0.004 0.0108 0.0048 0.0046 0.005 0.0025 0.0025
Nucleolus 0.0003 0.0005 0.0004 0.0002 0.0003 0.0002 0.0008 0.0004 0.0004 0.0001 0.0002 0.0002 0.0004 0.0001 0.0002 0.0021 0.0003 0.0002
Nucleus 0.0016 0.0097 0.0037 0.002 0.0012 0.0014 0.0044 0.0018 0.0032 0.0016 0.0009 0.0018 0.002 0.0011 0.0011 0.0016 0.0013 0.0015
Peroxisomes 0.0049 0.0019 0.0069 0.0031 0.0031 0.0058 0.0124 0.0028 0.0118 0.0013 0.0249 0.0056 0.0199 0.0008 0.0066 0.0042 0.0054 0.0074
Punctate Nuclear 0.0012 0.002 0.0022 0.0012 0.0035 0.0017 0.0046 0.0036 0.0021 0.001 0.0035 0.0017 0.0044 0.0007 0.0025 0.0011 0.0004 0.0014
Vacuole 0.0071 0.0093 0.0057 0.0187 0.0061 0.0051 0.0142 0.0085 0.0068 0.0074 0.0034 0.0073 0.0075 0.0047 0.0048 0.0101 0.0116 0.0056
Vacuole Periphery 0.0017 0.0018 0.002 0.0069 0.0019 0.0021 0.0029 0.0021 0.0021 0.0026 0.0013 0.0024 0.0022 0.0009 0.0009 0.0023 0.0049 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.3936 16.9416 18.6243 22.954 21.3864 21.7472 18.2399 18.8407 19.4459 31.3464
Translational Efficiency 1.0337 0.9463 0.8527 0.9521 1.0194 1.6071 0.8602 1.0706 1.1838 0.8664

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in recovery from arrest in response to pheromone; acts in a cell cycle arrest recovery pathway independent from Far1p; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
Localization
Cell Percentages ER (88%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Far8

Far8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Far8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available