Standard name | |
---|---|
Human Ortholog | |
Description | Component of the eisosome required for proper eisosome assembly; similar to Uso1p; authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress; EIS1 has a paralog, YKL050C, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 161 | 296 | 208 | 94 | 74 | 193 | 171 | – | 136 | 157 | 118 | 128 | 291 | 338 | 301 | 208 | 71 | 106 | 71 | 211 | 91 | 137 | 202 | 243 |
Bud | 18 | 29 | 20 | 15 | 6 | 27 | 30 | – | 8 | 22 | 31 | 17 | 38 | 53 | 36 | 7 | 1 | 6 | 0 | 2 | 1 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 3 | 7 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 156 | 271 | 170 | 79 | 57 | 139 | 108 | – | 104 | 109 | 80 | 91 | 351 | 432 | 364 | 207 | 174 | 107 | 192 | 331 | 194 | 264 | 381 | 399 |
Cytoplasm | 19 | 31 | 22 | 4 | 11 | 25 | 16 | – | 10 | 7 | 15 | 10 | 72 | 41 | 29 | 44 | 25 | 12 | 3 | 5 | 1 | 1 | 4 | 11 |
Endoplasmic Reticulum | 15 | 57 | 19 | 10 | 9 | 52 | 36 | – | 32 | 48 | 31 | 27 | 60 | 85 | 68 | 13 | 15 | 10 | 5 | 13 | 8 | 6 | 17 | 20 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 7 | 1 | 0 | 5 |
Golgi | 7 | 35 | 65 | 3 | 15 | 51 | 36 | – | 22 | 21 | 20 | 37 | 7 | 11 | 9 | 147 | 106 | 61 | 5 | 9 | 5 | 4 | 5 | 8 |
Mitochondria | 147 | 119 | 61 | 26 | 19 | 104 | 111 | – | 186 | 187 | 180 | 155 | 15 | 6 | 51 | 32 | 13 | 22 | 2 | 11 | 6 | 5 | 10 | 7 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 26 | 20 | 35 | 19 | 12 | 29 | 31 | – | 17 | 10 | 4 | 12 | 23 | 17 | 31 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 5 |
SpindlePole | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 3 | 0 | 2 | 0 | 0 | – | 0 | 0 | 2 | 1 | 0 | 2 | 0 | 2 | 2 | 0 | 3 | 4 | 3 | 0 | 2 | 0 |
Unique Cell Count | 273 | 490 | 293 | 128 | 109 | 322 | 298 | 295 | 307 | 261 | 251 | 520 | 630 | 551 | 397 | 232 | 200 | 289 | 602 | 334 | 430 | 639 | 721 | |
Labelled Cell Count | 550 | 860 | 605 | 251 | 206 | 621 | 540 | 517 | 564 | 484 | 480 | 858 | 985 | 890 | 663 | 408 | 327 | 289 | 602 | 334 | 430 | 639 | 721 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.6 | 13.3 | 10.5 | 10.0 | 10.8 | 11.0 | 11.1 | – | 11.4 | 11.1 | 11.0 | 12.0 | 17.1 | 18.7 | 18.4 | 19.0 | 21.7 | 19.9 | 11.7 | 12.5 | 12.6 |
Std Deviation (1e-4) | 1.7 | 2.6 | 1.5 | 1.6 | 1.6 | 1.9 | 1.6 | – | 2.1 | 2.1 | 2.5 | 2.4 | 3.6 | 4.3 | 4.3 | 5.0 | 7.6 | 5.4 | 2.2 | 2.8 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.05 | 0.07 | 0.07 | – | 0.12 | 0.08 | 0.07 | 0.2 | 0.7 | 0.83 | 0.81 | 0.86 | 1.05 | 0.92 | 0.16 | 0.26 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.5 | -0.6 | -2.9 | -3.4 | – | -6.1 | -5.0 | -6.2 | -4.8 | -4.2 | -5.0 | -4.6 | -4.9 | -9.2 | -4.1 |
Bud | 1.7 | -0.5 | 0.7 | 1.4 | – | -2.3 | 0.2 | 2.1 | 0 | 0.3 | 0.8 | -0.2 | -3.4 | -3.7 | -1.9 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.7 | -1.0 | -3.7 | -5.3 | – | -5.5 | -5.5 | -6.5 | -5.1 | 2.7 | 3.1 | 2.3 | -1.5 | 4.1 | -1.0 |
Cytoplasm | -1.7 | 0.8 | 0.1 | -1.1 | – | -2.2 | -3.0 | -0.8 | -1.7 | 2.7 | -0.6 | -1.3 | 1.6 | 1.3 | -0.6 |
Endoplasmic Reticulum | 0.5 | 0.6 | 3.7 | 2.3 | – | 1.9 | 3.6 | 2.2 | 1.8 | 2.3 | 3.1 | 2.7 | -2.0 | 0 | -0.7 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -5.1 | -1.9 | -2.0 | -3.3 | – | -5.0 | -5.4 | -4.7 | -2.2 | -10.0 | -10.5 | -10.0 | 4.2 | 5.7 | 2.1 |
Mitochondria | -0.1 | -0.8 | 3.2 | 4.4 | – | 10.4 | 10.0 | 11.4 | 9.7 | -8.4 | -10.8 | -4.7 | -4.9 | -5.0 | -2.9 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.8 | -0.3 | -1.2 | -0.6 | – | -2.6 | -4.0 | -4.8 | -3.0 | -4.0 | -5.7 | -3.3 | -6.8 | -5.4 | -4.8 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.4464 | 17.292 | 15.3559 | 14.984 | 14.3439 | 16.2924 | 14.5043 | 19.6563 | 15.8383 | 14.314 | 15.3094 | 17.8246 | 15.5591 | 16.4867 | 13.9498 | 15.2904 | 14.2186 | 15.7704 |
Actin | 0.0956 | 0.0689 | 0.0279 | 0.0337 | 0.0054 | 0.0186 | 0.082 | 0.0322 | 0.0399 | 0.0662 | 0.0828 | 0.0215 | 0.0108 | 0.0105 | 0.0039 | 0.0008 | 0.0008 | 0.0048 |
Bud | 0.0007 | 0.0002 | 0.0002 | 0.0013 | 0.0001 | 0.0001 | 0.0011 | 0.0002 | 0.0004 | 0.0021 | 0.0008 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 |
Bud Neck | 0.0022 | 0.0032 | 0.0031 | 0.0064 | 0.0013 | 0.003 | 0.0024 | 0.003 | 0.004 | 0.0076 | 0.0065 | 0.0014 | 0.0007 | 0.0008 | 0.0008 | 0.0005 | 0.0008 | 0.0016 |
Bud Periphery | 0.0048 | 0.0008 | 0.0014 | 0.0099 | 0.0014 | 0.0015 | 0.0054 | 0.001 | 0.0031 | 0.0114 | 0.0043 | 0.0037 | 0.0005 | 0.0003 | 0.0005 | 0.0004 | 0.0007 | 0.0011 |
Bud Site | 0.0083 | 0.0156 | 0.0083 | 0.0066 | 0.0007 | 0.0003 | 0.0084 | 0.039 | 0.0143 | 0.015 | 0.0072 | 0.0007 | 0.0014 | 0.0042 | 0.0027 | 0.0002 | 0.0001 | 0.0021 |
Cell Periphery | 0.1083 | 0.1111 | 0.1945 | 0.203 | 0.1616 | 0.1454 | 0.1031 | 0.192 | 0.2338 | 0.1462 | 0.2799 | 0.2151 | 0.0802 | 0.092 | 0.1063 | 0.0778 | 0.1392 | 0.137 |
Cytoplasm | 0.0043 | 0.0056 | 0.0045 | 0.0148 | 0.0028 | 0.0012 | 0.0073 | 0.0054 | 0.0094 | 0.0128 | 0.0048 | 0.0032 | 0.0012 | 0.0023 | 0.0019 | 0.0013 | 0.0031 | 0.0027 |
Cytoplasmic Foci | 0.0104 | 0.0005 | 0.0008 | 0.0003 | 0.0056 | 0.0001 | 0.0062 | 0.0002 | 0.0068 | 0.0056 | 0.0026 | 0.0019 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Eisosomes | 0.7095 | 0.7754 | 0.733 | 0.6914 | 0.7375 | 0.8098 | 0.6976 | 0.7156 | 0.6523 | 0.6598 | 0.5179 | 0.7132 | 0.9009 | 0.8835 | 0.877 | 0.9078 | 0.8247 | 0.8374 |
Endoplasmic Reticulum | 0.0069 | 0.0037 | 0.0033 | 0.0079 | 0.0094 | 0.0054 | 0.0113 | 0.006 | 0.0034 | 0.0084 | 0.0106 | 0.01 | 0.0015 | 0.0027 | 0.0035 | 0.0078 | 0.0249 | 0.0067 |
Endosome | 0.0033 | 0.0002 | 0.0045 | 0.0002 | 0.0032 | 0.0003 | 0.0132 | 0.0001 | 0.0041 | 0.0115 | 0.0251 | 0.0029 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Golgi | 0.0112 | 0.0122 | 0.0049 | 0.0206 | 0.003 | 0.0043 | 0.0161 | 0.002 | 0.0024 | 0.0163 | 0.0092 | 0.0098 | 0.0007 | 0.0017 | 0.0004 | 0.0021 | 0.0018 | 0.0026 |
Lipid Particles | 0.0063 | 0.0006 | 0.0014 | 0.0007 | 0.0291 | 0.0003 | 0.0028 | 0.0002 | 0.0026 | 0.0049 | 0.0026 | 0.0016 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0012 | 0.0005 |
Mitochondria | 0.0064 | 0.0008 | 0.0021 | 0.0005 | 0.0086 | 0.008 | 0.0139 | 0.0015 | 0.0051 | 0.021 | 0.037 | 0.006 | 0.0009 | 0.0004 | 0.0013 | 0.0001 | 0.0002 | 0.0007 |
None | 0.004 | 0.0005 | 0.0012 | 0.0005 | 0.0176 | 0.0006 | 0.0047 | 0.0005 | 0.0031 | 0.0016 | 0.001 | 0.001 | 0.0005 | 0.0004 | 0.0005 | 0.0005 | 0.0005 | 0.0006 |
Nuclear Periphery | 0.009 | 0.0003 | 0.0014 | 0.0005 | 0.0009 | 0.0004 | 0.0112 | 0.0005 | 0.0015 | 0.0032 | 0.0016 | 0.0019 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0008 | 0.0003 |
Nucleolus | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0003 | 0 | 0.0007 | 0.0004 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0006 |
Nucleus | 0.0019 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0057 | 0.0001 | 0.0026 | 0.0008 | 0.0005 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0003 |
Peroxisomes | 0.0031 | 0.0001 | 0.0005 | 0.0001 | 0.0094 | 0.0001 | 0.0011 | 0.0001 | 0.0049 | 0.0011 | 0.0008 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0019 | 0 | 0.0007 | 0 | 0.0007 | 0 | 0.003 | 0 | 0.0037 | 0.0009 | 0.0001 | 0.004 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.001 | 0.0002 | 0.0047 | 0.0014 | 0.0009 | 0.0003 | 0.0017 | 0.0006 | 0.0016 | 0.0026 | 0.0035 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0009 | 0.0006 |
Vacuole Periphery | 0.0006 | 0 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0015 | 0 | 0.0003 | 0.0008 | 0.0011 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.6377 | 17.5304 | 41.6266 | 43.7125 | 12.1478 | 16.9862 | 31.511 | 52.6378 | 56.6877 | 16.7971 |
Translational Efficiency | 2.9844 | 1.9266 | 2.4399 | 2.8151 | 1.9623 | 2.9567 | 1.8852 | 2.0397 | 1.8844 | 1.9657 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2544 | 1467 | 1915 | 1555 | 2157 | 2480 | 2510 | 2706 | 4701 | 3947 | 4425 | 4261 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1039.74 | 1042.18 | 1631.19 | 1822.72 | 1022.13 | 1052.01 | 1569.11 | 2001.67 | 1031.66 | 1048.36 | 1595.98 | 1936.36 |
Standard Deviation | 137.75 | 182.53 | 278.72 | 324.10 | 163.22 | 148.11 | 249.20 | 348.10 | 150.23 | 161.83 | 264.18 | 350.29 |
Intensity Change Log 2 | — | 0.003382 | 0.649702 | 0.809870 | — | 0.041570 | 0.618368 | 0.969625 | — | 0.022439 | 0.634254 | 0.891276 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.066716 | 0.136463* | 0.043584 | 0.080413 | 0.065192 | 0.094117 | 0.069869 | 0.030204 | 0.066017 | 0.109856* | 0.058493 | 0.048527 |
Bud Neck | 0.001865 | 0.005258 | 0.008392 | 0.005834 | 0.002668 | 0.002754 | 0.003571 | 0.001545 | 0.002234 | 0.003685 | 0.005657 | 0.003110 |
Bud Site | 0.000500 | 0.003662 | 0.003568 | 0.011235 | 0.001036 | 0.002294 | 0.002473 | 0.002209 | 0.000746 | 0.002802 | 0.002946 | 0.005503 |
Cell Periphery | 0.072190 | 0.171776* | 0.333320* | 0.497031* | 0.202754* | 0.339429* | 0.206111* | 0.751673* | 0.132098* | 0.277117* | 0.261163* | 0.658745* |
Cytoplasm | 0.000082 | 0.000473 | 0.000076 | 0.000846 | 0.000135 | 0.000715 | 0.000349 | 0.000819 | 0.000106 | 0.000625 | 0.000231 | 0.000829 |
Cytoplasmic Foci | 0.001601 | 0.001304 | 0.000224 | 0.000576 | 0.001653 | 0.001364 | 0.001341 | 0.000187 | 0.001625 | 0.001342 | 0.000858 | 0.000329 |
Eisosomes | 0.841894* | 0.624350* | 0.591707* | 0.383118* | 0.705025* | 0.508004* | 0.580518* | 0.207771* | 0.779093* | 0.551247* | 0.585360* | 0.271762* |
Endoplasmic Reticulum | 0.001662 | 0.017313 | 0.009790 | 0.006813 | 0.003417 | 0.004688 | 0.027725 | 0.000776 | 0.002468 | 0.009380 | 0.019963 | 0.002979 |
Endosome | 0.001257 | 0.007601 | 0.001804 | 0.002194 | 0.002691 | 0.003224 | 0.008922 | 0.000732 | 0.001915 | 0.004850 | 0.005841 | 0.001266 |
Golgi | 0.002971 | 0.011103 | 0.000651 | 0.000946 | 0.003289 | 0.002911 | 0.029002 | 0.000340 | 0.003117 | 0.005956 | 0.016733 | 0.000562 |
Lipid Particles | 0.001862 | 0.007121 | 0.002663 | 0.003417 | 0.002119 | 0.003879 | 0.042518 | 0.000279 | 0.001980 | 0.005084 | 0.025270 | 0.001424 |
Mitochondria | 0.000504 | 0.001632 | 0.000932 | 0.002500 | 0.000634 | 0.001455 | 0.000600 | 0.000888 | 0.000564 | 0.001521 | 0.000744 | 0.001476 |
Mitotic Spindle | 0.000797 | 0.001365 | 0.000982 | 0.001439 | 0.001298 | 0.001062 | 0.000742 | 0.000270 | 0.001027 | 0.001175 | 0.000846 | 0.000697 |
None | 0.001314 | 0.000539 | 0.000204 | 0.000173 | 0.000808 | 0.000536 | 0.000278 | 0.000116 | 0.001082 | 0.000537 | 0.000246 | 0.000137 |
Nuclear Periphery | 0.000226 | 0.000371 | 0.000658 | 0.000396 | 0.000402 | 0.000367 | 0.000603 | 0.000198 | 0.000306 | 0.000369 | 0.000627 | 0.000270 |
Nuclear Periphery Foci | 0.000004 | 0.000032 | 0.000035 | 0.000323 | 0.000015 | 0.000015 | 0.000032 | 0.000005 | 0.000009 | 0.000021 | 0.000033 | 0.000121 |
Nucleolus | 0.000003 | 0.000007 | 0.000020 | 0.000026 | 0.000004 | 0.000004 | 0.000005 | 0.000007 | 0.000003 | 0.000005 | 0.000011 | 0.000014 |
Nucleus | 0.000084 | 0.000148 | 0.000140 | 0.000166 | 0.000110 | 0.000123 | 0.000103 | 0.000053 | 0.000096 | 0.000132 | 0.000119 | 0.000094 |
Peroxisomes | 0.004428 | 0.009260 | 0.000691 | 0.000723 | 0.006637 | 0.032953 | 0.024582 | 0.001836 | 0.005442 | 0.024147 | 0.014243 | 0.001430 |
Vacuole | 0.000025 | 0.000144 | 0.000508 | 0.001384 | 0.000077 | 0.000065 | 0.000401 | 0.000061 | 0.000049 | 0.000094 | 0.000447 | 0.000543 |
Vacuole Periphery | 0.000013 | 0.000078 | 0.000054 | 0.000446 | 0.000035 | 0.000040 | 0.000254 | 0.000032 | 0.000023 | 0.000054 | 0.000167 | 0.000183 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.69 | 5.16 | -2.44 | 7.14 | -6.75 | -5.18 | -0.91 | 7.95 | 13.73 | 9.58 | -10.17 | 2.18 | 5.25 | 14.77 | 3.07 |
Bud Neck | -11.46 | -14.24 | -11.97 | -1.39 | 4.70 | -0.43 | -3.27 | 5.81 | 6.71 | 7.75 | -8.97 | -13.54 | -5.22 | 2.95 | 9.26 |
Bud Site | -5.05 | -6.95 | -7.42 | -4.83 | -5.10 | -3.01 | -3.09 | -2.58 | 0.15 | 0.45 | -6.00 | -6.87 | -7.93 | -4.00 | -3.85 |
Cell Periphery | -14.40 | -34.50* | -46.71* | -30.49* | -14.73 | -16.45 | -0.43 | -69.52* | -50.15* | -70.88* | -25.70 | -23.80 | -88.05 | -55.39* | -59.25* |
Cytoplasm | -2.83 | 0.16 | -4.34 | -1.70 | -4.36 | -5.32 | -2.55 | -3.00 | -0.42 | -1.95 | -6.08 | -2.52 | -4.58 | -1.16 | -3.66 |
Cytoplasmic Foci | 0.95 | 6.51 | 3.23 | 2.27 | -1.55 | 0.87 | 0.87 | 4.58 | 11.84 | 7.08 | 1.33 | 3.71 | 6.32 | 7.61 | 4.12 |
Eisosomes | 22.27* | 29.87 | 50.06* | 20.46 | 19.57 | 21.75* | 13.37 | 61.23* | 37.64* | 45.06* | 34.87* | 30.83 | 85.12* | 40.91* | 47.56* |
Endoplasmic Reticulum | -8.90 | -8.55 | -6.18 | 5.53 | 2.49 | -2.07 | -12.92 | 6.11 | 7.62 | 14.57 | -9.14 | -15.24 | -1.30 | 8.19 | 14.58 |
Endosome | -9.66 | -3.80 | -3.12 | 7.57 | -1.31 | -1.73 | -10.08 | 6.92 | 16.19 | 14.66 | -10.04 | -11.22 | 3.60 | 12.63 | 13.34 |
Golgi | -5.72 | 4.28 | 3.30 | 7.52 | -1.00 | 0.65 | -12.68 | 5.93 | 5.32 | 14.32 | -4.33 | -11.37 | 6.36 | 9.14 | 13.91 |
Lipid Particles | -5.95 | -1.27 | -1.78 | 3.16 | -0.77 | -2.63 | -16.26 | 4.20 | 6.97 | 17.25 | -6.03 | -15.93 | 1.38 | 6.77 | 16.20 |
Mitochondria | -3.40 | -6.46 | -3.61 | -1.35 | -2.83 | -2.46 | 0.27 | -1.45 | 1.58 | -1.55 | -3.97 | -2.46 | -3.99 | 0.13 | -3.14 |
Mitotic Spindle | -4.12 | -2.92 | -1.25 | -0.34 | -0.96 | 2.62 | 6.48 | 13.20 | 16.30* | 11.64 | -2.07 | 3.46 | 1.20 | 1.81 | 0.42 |
None | 7.50 | 11.54 | 11.85 | 9.43 | 2.93 | 5.40 | 11.52 | 16.55 | 14.36 | 7.91 | 9.08 | 14.75 | 17.00 | 17.14 | 8.76 |
Nuclear Periphery | -6.96 | -15.71 | -7.83 | -0.99 | 8.05 | 1.41 | -5.88 | 9.56 | 9.06 | 13.30 | -3.96 | -14.44 | 2.51 | 6.32 | 16.17 |
Nuclear Periphery Foci | -7.28 | -8.71 | -1.33 | -1.24 | -1.23 | -0.14 | -5.14 | 3.73 | 9.44 | 12.76 | -6.24 | -10.63 | -1.29 | -1.17 | -1.06 |
Nucleolus | -1.91 | -2.11 | -2.52 | -2.01 | -0.48 | -0.28 | -2.46 | -7.58 | -7.17 | -5.80 | -2.13 | -2.26 | -3.09 | -2.49 | -0.49 |
Nucleus | -8.13 | -8.76 | -3.38 | -0.73 | -1.09 | -2.31 | 1.31 | 11.95 | 16.47 | 13.52 | -8.24 | -5.87 | 0.13 | 3.90 | 2.59 |
Peroxisomes | -4.36 | 7.39 | 8.25 | 8.32 | -0.11 | -12.57 | -10.39 | 6.23 | 15.55 | 14.11 | -13.67 | -8.78 | 8.91 | 17.13 | 13.57 |
Vacuole | -6.88 | -10.71 | -3.02 | -2.76 | -1.96 | 0.99 | -2.69 | 0.97 | 0.31 | 2.83 | -5.02 | -5.62 | -3.00 | -2.73 | -0.58 |
Vacuole Periphery | -4.03 | -7.82 | -2.05 | -1.74 | -1.86 | -0.51 | -1.68 | 0.19 | 0.75 | 1.70 | -3.92 | -1.96 | -2.07 | -1.67 | -0.15 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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