Standard name
Human Ortholog
Description Protein that regulates actin cytoskeleton organization; required for cytokinesis, actin cable organization, and secretory vesicle trafficking; localized to bud neck; phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; contains an SH3 domain; N terminus controls cell size and levels of actin cables, while C terminus controls actin cable organization via direct regulation of the formin Bnr1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.12 0 0 0.05 0.08 0 0 0
Bud 0 0 0 0.07 0 0 0 0 0.06 0 0.07 0 0 0 0 – 0 0 0.13 0.15 0.18 0.09 0 0
Bud Neck 0.06 0.1 0.06 0 0.09 0.1 0.12 0.13 0.06 0.08 0 0.05 0 0.06 0.09 – 0.12 0.06 0.24 0.3 0.21 0.18 0 0
Bud Site 0.08 0.09 0.06 0 0 0.09 0 0 0 0 0 0 0 0 0 – 0.18 0.19 – – – – – –
Cell Periphery 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.64 0.58 0.82 0.83 0.75 0.66 0.74 0.7 0.63 0.71 0.43 0.6 0.91 0.81 0.69 – 0.47 0.19 0.37 0.28 0.26 0.52 0.78 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0.06 0 0 0.07 0.07 0.09 0.31 0.2 0.56 0.36 0 0 0 – 0.18 0.12 0.07 0.05 0.16 0 0 0.05
Nucleus 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Peroxisomes 0.08 0.06 0 0 0 0.07 0 0 0 0 0 0 0 0 0.08 – 0.06 0.12 0 0 0 0 0 0
SpindlePole 0.12 0.08 0 0.1 0.11 0.13 0.12 0.14 0.05 0.08 0 0.11 0 0.11 0.17 – 0.18 0.31 0.09 0.07 0 0.06 0 0
Vac/Vac Membrane 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 7 0 0 6 3 2 8 1 0 1 0 4 8 1 – 2 0 4 10 6 2 6 5
Bud 2 7 0 2 4 1 8 6 10 7 6 7 0 3 3 – 0 0 18 32 14 11 8 8
Bud Neck 7 18 1 1 16 9 24 26 9 14 4 8 3 11 18 – 2 1 34 66 16 23 6 0
Bud Site 9 16 1 0 3 8 8 2 2 3 0 5 2 6 2 – 3 3 – – – – – –
Cell Periphery 0 2 0 2 2 1 0 0 0 1 0 0 0 0 1 – 0 0 0 0 0 0 0 0
Cytoplasm 75 104 14 25 129 57 153 139 98 119 38 97 108 151 145 – 8 3 53 62 20 66 198 171
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 – 1 0 1 0 0 0 0 1
Endosome 5 9 0 0 1 1 2 3 1 0 0 3 2 3 7 – 0 1 2 5 0 3 2 3
Golgi 0 0 0 1 0 0 0 0 0 0 0 2 0 0 2 – 0 0 0 0 0 1 0 1
Mitochondria 9 3 1 0 4 6 14 18 48 34 50 59 0 1 0 – 3 2 9 11 12 3 10 11
Nucleus 2 2 1 0 4 1 3 4 4 1 0 4 2 0 0 – 0 1 0 1 0 0 0 1
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0
Nucleolus 3 3 0 1 1 1 3 5 1 1 3 1 0 0 0 – 1 0 0 2 0 0 0 0
Peroxisomes 9 10 0 0 3 6 1 2 1 0 1 4 0 2 17 – 1 2 0 0 1 0 0 0
SpindlePole 14 15 0 3 18 11 24 28 7 14 4 18 2 20 36 – 3 5 12 15 2 7 5 3
Vac/Vac Membrane 2 10 1 0 2 0 3 0 5 3 0 3 0 1 2 – 0 1 3 7 0 3 3 1
Unique Cell Count 118 178 17 30 171 86 207 199 155 168 89 163 119 186 211 17 16 144 220 78 126 255 216
Labelled Cell Count 141 207 19 35 193 105 245 241 187 197 108 211 123 207 235 24 19 144 220 78 126 255 216


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.9 5.0 5.9 5.5 4.7 4.4 4.8 3.6 3.8 3.4 4.1 5.6 5.1 5.3 – 6.3 6.1 5.5 5.4 5.8
Std Deviation (1e-4) 0.8 1.1 0.9 2.2 2.0 2.4 1.6 1.5 0.8 0.9 0.6 2.7 1.5 1.2 1.9 – 1.6 1.9 1.6 1.6 2.1
Intensity Change (Log2) – – – 0.23 0.14 -0.1 -0.18 -0.07 -0.49 -0.38 -0.57 -0.28 0.15 0.03 0.07 – 0.32 0.27 0.14 0.1 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cytoplasm 0.1 -0.6 -1.3 -0.8 -1.1 -1.6 -1.0 -3.0 -1.8 1.1 -0.1 -1.2 – -2.2 -3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0353 0.0024 0.0119 0.0015 0.0137 0.005 0.0097 0.0038 0.0067 0.0002 0.0006 0.0017 0.0765 0.0205 0.0443 0.0001 0.0006 0.0008
Bud 0.0064 0.0132 0.0076 0.0003 0.0025 0.0036 0.0002 0.0143 0.0009 0 0.0003 0.0003 0.0039 0.0046 0.0036 0 0.0002 0.0023
Bud Neck 0.0608 0.0154 0.2357 0.8977 0.7159 0.7668 0.0006 0.012 0.151 0.5455 0.7947 0.657 0.1008 0.0073 0.1937 0.5984 0.9901 0.7799
Bud Periphery 0.0102 0.0196 0.011 0.0004 0.0034 0.0029 0.0002 0.0131 0.0014 0.0001 0.0006 0.0009 0.0056 0.0035 0.0068 0.0001 0.0001 0.0015
Bud Site 0.099 0.1659 0.0686 0.007 0.0146 0.0005 0.0012 0.1682 0.0735 0.0005 0.0015 0.0004 0.2082 0.2026 0.1187 0.0006 0.0069 0.0008
Cell Periphery 0.002 0.0016 0.0022 0.0003 0.0019 0.0003 0.0002 0.0036 0.002 0.0002 0.0006 0.0004 0.0083 0.0016 0.0043 0.0001 0.0003 0.0002
Cytoplasm 0.0313 0.0921 0.0895 0.0045 0.0138 0.0522 0.0266 0.0956 0.0499 0.0028 0.028 0.0055 0.0449 0.1846 0.1661 0.0019 0.0004 0.1367
Cytoplasmic Foci 0.1231 0.0291 0.0311 0.0088 0.1053 0.0276 0.0515 0.0173 0.0201 0.0003 0.0011 0.0244 0.1659 0.0416 0.0822 0.0112 0.0009 0.0412
Eisosomes 0.0009 0.0004 0.0003 0 0.0002 0.0001 0.0002 0.0003 0.0005 0.0001 0 0 0.0014 0.0002 0.0008 0 0 0
Endoplasmic Reticulum 0.0054 0.0022 0.0019 0.0019 0.0004 0.0002 0.0079 0.0042 0.0028 0.004 0.0126 0.0008 0.0111 0.0033 0.0014 0.0005 0 0.0013
Endosome 0.0273 0.0051 0.0082 0.0155 0.0128 0.0063 0.0983 0.0097 0.0166 0.0001 0.0015 0.0143 0.0994 0.0046 0.0078 0.1593 0 0.0093
Golgi 0.0077 0.0018 0.0052 0.0011 0.0105 0.0013 0.0203 0.0011 0.0038 0 0.0001 0.0097 0.0296 0.0078 0.0046 0.0233 0.0001 0.0016
Lipid Particles 0.0202 0.0018 0.0075 0.0044 0.0054 0.003 0.0905 0.0059 0.0069 0 0 0.0141 0.0369 0.0011 0.0025 0.0017 0 0.0035
Mitochondria 0.0095 0.0039 0.0023 0.0005 0.0042 0.0018 0.0039 0.0017 0.0022 0.0001 0.0012 0.006 0.0038 0.0041 0.0009 0.001 0 0.0009
None 0.4615 0.6111 0.4757 0.0021 0.0208 0.1177 0.3137 0.541 0.4773 0.0865 0.0554 0.0359 0.0919 0.5008 0.3479 0.1987 0 0.0123
Nuclear Periphery 0.034 0.0011 0.0052 0.0096 0.0002 0.0002 0.326 0.0805 0.1531 0.3474 0.0691 0.2178 0.0152 0.0021 0.0002 0.0002 0 0.0004
Nucleolus 0.007 0.0028 0.0032 0.0311 0.0012 0.0009 0.0004 0.0049 0.0012 0.0001 0.0003 0.0003 0.0105 0.0006 0.0006 0.0001 0.0001 0.0006
Nucleus 0.0134 0.0036 0.0059 0.0031 0.002 0.0038 0.003 0.0041 0.0034 0.0023 0.029 0.0008 0.0128 0.0019 0.0012 0.0001 0.0001 0.0007
Peroxisomes 0.021 0.0098 0.0075 0.0015 0.0521 0.0014 0.019 0.0047 0.0118 0 0 0.0029 0.0149 0.0028 0.009 0.0002 0 0.002
Punctate Nuclear 0.0179 0.0142 0.0145 0.0073 0.0183 0.0028 0.0125 0.0055 0.0015 0 0.0006 0.0005 0.0432 0.0023 0.0026 0.0001 0.0002 0.0021
Vacuole 0.0042 0.0022 0.0042 0.0008 0.0007 0.0012 0.0028 0.0038 0.0025 0.0003 0.0012 0.0017 0.0129 0.0018 0.0007 0.0014 0 0.0016
Vacuole Periphery 0.0019 0.0003 0.0007 0.0005 0.0001 0.0003 0.0114 0.0045 0.011 0.0095 0.0017 0.0045 0.0022 0.0004 0.0001 0.0011 0 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.6469 14.722 32.7731 22.5388 6.0499 3.8406 22.8243 37.0052 26.8332 8.1135
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Protein that regulates actin cytoskeleton organization; required for cytokinesis, actin cable organization, and secretory vesicle trafficking; localized to bud neck; phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; contains an SH3 domain; N terminus controls cell size and levels of actin cables, while C terminus controls actin cable organization via direct regulation of the formin Bnr1p
Localization
Cell Percentages ER (48%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Hof1

Hof1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hof1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available